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The map label for this gene is truA [H]

Identifier: 220917510

GI number: 220917510

Start: 2671064

End: 2671882

Strand: Direct

Name: truA [H]

Synonym: A2cp1_2410

Alternate gene names: 220917510

Gene position: 2671064-2671882 (Clockwise)

Preceding gene: 220917505

Following gene: 220917520

Centisome position: 53.11

GC content: 78.27

Gene sequence:

>819_bases
ATGGAACGGCGCTCCTACGCACTGCGGCTCGCCTACGACGGCGGCCGGTTCCGGGGATTCCAGCGGCAGCCCGGGCTGCC
GACCGTGCAGCAGGCGCTGGAGGAGGCGCTGGAGCGGCTCGGGGTGCGGGCCCAGCTCCACGTCGCGGCCCGCACCGACG
CCGGCGTGCACGCCCTCGCGCAGGTCGTCAGCTTCTCCGCGCGCGCCGCGCTCGACCCGGTGGCGCTGCGGGGGGGACTG
AACGAGGGCCTGCCCGAGGGGATCCTGGTGCTGGACGCGGCGCGGGTCGGCCCGTCGTTCCACGCGCGCGGCTCGGCGAT
CGCGCGCACCTACGTCTACCTCGTGGGCGCTCCGCCGCCGGTGGGGCTGCGCCCGTACGCGTGGACGCTGCCCGACGCGC
GCGCGTTCCCGGACCTCGCCGAGGTCCCGCCCCTCGACGCCGCGGCCATGCGCGAGGCGCTCGGGCACGCGGTCGGCGAG
CACGACTTCGTCGGCTTCGCCCGCCCCGGCGAGCAGCGCGGCACGGTGCGGACCCTGCTCCGCGCCGAGGTGATCGAGGC
GGGCTGGGCACCGCTCGTGGCGGTGGTGCTGGAGGGCAAGGGGTTCCTGCGCGCGATGGTGCGGAACCTGGTGGGCACCG
CGGTCGCGGCCGGCCTCGGCCGCGCCCCGCCGGACGTGATCCGCACCCTGCTCGCGACGCGCGGCCGCTACCGCGGCGTC
CGCGCGCCGGGCTGGGGGCTCACGCTCGCAGCGGTGCGCTATCCGGACGGTACGTGGCCGTCGCGCGCGGCGCCGCCGGA
CCCGGCAGCGCCTCGATGA

Upstream 100 bases:

>100_bases
TCGGTGCCTGGCGCCATGCGGTGCGGGGACGTGGGGTGAGGTCGGAGGCGCGCGGCCGGCTCGCGCCGGCGCGGCGGGCG
TGGGAACTTAGCACGTCGGC

Downstream 100 bases:

>100_bases
TCCCGTCGGCGCGGCGCACCACCGCGACGCGGACCAGGCGCAGGCGGGCCAGGGTCCGGACGGGCAGCGGCGGCGGGCGG
GCCGACTCGGCGCGCTCGCG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MERRSYALRLAYDGGRFRGFQRQPGLPTVQQALEEALERLGVRAQLHVAARTDAGVHALAQVVSFSARAALDPVALRGGL
NEGLPEGILVLDAARVGPSFHARGSAIARTYVYLVGAPPPVGLRPYAWTLPDARAFPDLAEVPPLDAAAMREALGHAVGE
HDFVGFARPGEQRGTVRTLLRAEVIEAGWAPLVAVVLEGKGFLRAMVRNLVGTAVAAGLGRAPPDVIRTLLATRGRYRGV
RAPGWGLTLAAVRYPDGTWPSRAAPPDPAAPR

Sequences:

>Translated_272_residues
MERRSYALRLAYDGGRFRGFQRQPGLPTVQQALEEALERLGVRAQLHVAARTDAGVHALAQVVSFSARAALDPVALRGGL
NEGLPEGILVLDAARVGPSFHARGSAIARTYVYLVGAPPPVGLRPYAWTLPDARAFPDLAEVPPLDAAAMREALGHAVGE
HDFVGFARPGEQRGTVRTLLRAEVIEAGWAPLVAVVLEGKGFLRAMVRNLVGTAVAAGLGRAPPDVIRTLLATRGRYRGV
RAPGWGLTLAAVRYPDGTWPSRAAPPDPAAPR
>Mature_272_residues
MERRSYALRLAYDGGRFRGFQRQPGLPTVQQALEEALERLGVRAQLHVAARTDAGVHALAQVVSFSARAALDPVALRGGL
NEGLPEGILVLDAARVGPSFHARGSAIARTYVYLVGAPPPVGLRPYAWTLPDARAFPDLAEVPPLDAAAMREALGHAVGE
HDFVGFARPGEQRGTVRTLLRAEVIEAGWAPLVAVVLEGKGFLRAMVRNLVGTAVAAGLGRAPPDVIRTLLATRGRYRGV
RAPGWGLTLAAVRYPDGTWPSRAAPPDPAAPR

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=287, Percent_Identity=34.8432055749129, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI199559785, Length=291, Percent_Identity=27.4914089347079, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70166645, Length=271, Percent_Identity=26.1992619926199, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI70166634, Length=271, Percent_Identity=26.1992619926199, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI70166599, Length=271, Percent_Identity=26.1992619926199, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788657, Length=253, Percent_Identity=36.3636363636364, Blast_Score=121, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6325044, Length=133, Percent_Identity=31.5789473684211, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648455, Length=271, Percent_Identity=27.6752767527675, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281362146, Length=271, Percent_Identity=27.6752767527675, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI116007752, Length=304, Percent_Identity=28.6184210526316, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28914; Mature: 28914

Theoretical pI: Translated: 11.04; Mature: 11.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRSYALRLAYDGGRFRGFQRQPGLPTVQQALEEALERLGVRAQLHVAARTDAGVHALA
CCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHH
QVVSFSARAALDPVALRGGLNEGLPEGILVLDAARVGPSFHARGSAIARTYVYLVGAPPP
HHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEHHHCCCCCCCCCHHEEEEEEEEEECCCC
VGLRPYAWTLPDARAFPDLAEVPPLDAAAMREALGHAVGEHDFVGFARPGEQRGTVRTLL
CCCCCCEEECCCHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
RAEVIEAGWAPLVAVVLEGKGFLRAMVRNLVGTAVAAGLGRAPPDVIRTLLATRGRYRGV
HHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
RAPGWGLTLAAVRYPDGTWPSRAAPPDPAAPR
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MERRSYALRLAYDGGRFRGFQRQPGLPTVQQALEEALERLGVRAQLHVAARTDAGVHALA
CCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHH
QVVSFSARAALDPVALRGGLNEGLPEGILVLDAARVGPSFHARGSAIARTYVYLVGAPPP
HHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEHHHCCCCCCCCCHHEEEEEEEEEECCCC
VGLRPYAWTLPDARAFPDLAEVPPLDAAAMREALGHAVGEHDFVGFARPGEQRGTVRTLL
CCCCCCEEECCCHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
RAEVIEAGWAPLVAVVLEGKGFLRAMVRNLVGTAVAAGLGRAPPDVIRTLLATRGRYRGV
HHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
RAPGWGLTLAAVRYPDGTWPSRAAPPDPAAPR
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA