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The map label for this gene is pleD [H]

Identifier: 220917497

GI number: 220917497

Start: 2654919

End: 2656223

Strand: Direct

Name: pleD [H]

Synonym: A2cp1_2397

Alternate gene names: 220917497

Gene position: 2654919-2656223 (Clockwise)

Preceding gene: 220917495

Following gene: 220917500

Centisome position: 52.79

GC content: 77.85

Gene sequence:

>1305_bases
ATGAGCTCCGTGAACGGACACCCCGAGGGCCCGCCCTCCGACGTCCCCGCCTCCGACGCGCTGGCGCGCCTCGCGGACGC
GCTCGCGGCGGCGGGGGGCGGCGCGCCCGCCGAGATCGCGCGCGCGCTCCGGGCCGCGCTGCCGGCCGCGGTGGCCGAGC
TCGCCGATGCCCAGCGCCATCTGGCCGAGTCGGCGGCGCGGCGCGACGCGCTCGTGGCCACCGCCGCGGACGAGCTTCGG
GCCGCGGCCCGCCTGCTCGAGCCCGACGGCGGGCCCGCCCGCGCCGAGCAGGTGCGCCGGCTGCTCGAGCTCGCCGACGA
GCTGGATCGCGCCCGCTCGCCCGAGGCCGCCGAGGGCCACGCGGCCCCGAGGCGCCGCGGCGAGCGCCCGCTCCTGCTGG
TGGCCGACGACGAGCCCGACGCCCGCGAGGTGCTGTCGCAGGTGCTCGCGCCCGAGTACGAGGTCGCGTCCGCCTCCGAC
GGCCAGGAGGCGCTGGACCTCGCCCGCGCCGAGCACCCGGACCTGGTGCTGCTCGACATCGGCATGCCGCGGCTGGACGG
CTTCGAGGTGCTGCAGCGGCTCCGCCAGGACCCGGCCACGGCCGACATCCCGGTGATCTTCGTGTCGGGGCGCGGCGACG
ACGCGGTGAAGGTGCGCAGCCTCGATCTCGGCGCGGTCGACTACCTCCAGAAGCCGTTCTCCGAGCGCGAGCTGCGCGCG
CGGGTGGAGCGGACGCTGCGCCTGGTCCGCTCCCAGATCGCGCTCCGGGAGCTCGCCCAGACCGACGCGCTCACCGGCCT
CGCGAACCTGCGCGCGTTCCGCGCCCGGGTGGACGAGGAGGTGAAGCGGGCCCGCCGCTACCGGACGCCGCTCACCTGCG
TCATGGCGGACATGGACAACCTGAAGCCCATCAACGACGCGCTGGGCCACGCCGCCGGCGACCGCGCCATCGCCGCCGTG
GCGGCGGTGATCCGGGAGGAGCTGCGCGAGACCGACTTCGGCGCGCGCTACGGCGGCGACGAGTTCGTGCTCCTGCTCCC
GCACACCACCGGCGAGGAGGGGCACGTGTTCGCCGAGCGCGTCTGCACGCGCCTGCACGAGACCGTGCTGGAGGTGTCCG
GGGAGCGGGTCCCGCTGGGCGCCTCCTTCGGCGTGGCCTGCCTGCCGGACGAGGACGGCGACGACGGGGGCGCGGAAGCC
CTGGTGCGCGCGGCCGACGCGGCGCTGTACGTGGCGAAGCGGGCCGGGCGCGGGCGGGTGGCGCTCGCGGCCACCGAGCC
CCACGCCTCGGCCAGCGCCGGCTAG

Upstream 100 bases:

>100_bases
GGCGGGGCTGCTGCCCGGGCGAGCGCCCGAAGGCTGCCCGGAAGGCCGACTGCTGGGCGACGGCGGCCCCCCTAGATTCA
AGGGGATCCCCGGTTGCCGC

Downstream 100 bases:

>100_bases
GCCTTCGCGCCTCCCGCCGCGGCGGGATCGCCCTCCGACGCCTCGCCGCGCGACCGGGCCTCGGCCGCGTAGCGGTTCAG
CTTGTTGTAGAGCGTCTTCT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSSVNGHPEGPPSDVPASDALARLADALAAAGGGAPAEIARALRAALPAAVAELADAQRHLAESAARRDALVATAADELR
AAARLLEPDGGPARAEQVRRLLELADELDRARSPEAAEGHAAPRRRGERPLLLVADDEPDAREVLSQVLAPEYEVASASD
GQEALDLARAEHPDLVLLDIGMPRLDGFEVLQRLRQDPATADIPVIFVSGRGDDAVKVRSLDLGAVDYLQKPFSERELRA
RVERTLRLVRSQIALRELAQTDALTGLANLRAFRARVDEEVKRARRYRTPLTCVMADMDNLKPINDALGHAAGDRAIAAV
AAVIREELRETDFGARYGGDEFVLLLPHTTGEEGHVFAERVCTRLHETVLEVSGERVPLGASFGVACLPDEDGDDGGAEA
LVRAADAALYVAKRAGRGRVALAATEPHASASAG

Sequences:

>Translated_434_residues
MSSVNGHPEGPPSDVPASDALARLADALAAAGGGAPAEIARALRAALPAAVAELADAQRHLAESAARRDALVATAADELR
AAARLLEPDGGPARAEQVRRLLELADELDRARSPEAAEGHAAPRRRGERPLLLVADDEPDAREVLSQVLAPEYEVASASD
GQEALDLARAEHPDLVLLDIGMPRLDGFEVLQRLRQDPATADIPVIFVSGRGDDAVKVRSLDLGAVDYLQKPFSERELRA
RVERTLRLVRSQIALRELAQTDALTGLANLRAFRARVDEEVKRARRYRTPLTCVMADMDNLKPINDALGHAAGDRAIAAV
AAVIREELRETDFGARYGGDEFVLLLPHTTGEEGHVFAERVCTRLHETVLEVSGERVPLGASFGVACLPDEDGDDGGAEA
LVRAADAALYVAKRAGRGRVALAATEPHASASAG
>Mature_433_residues
SSVNGHPEGPPSDVPASDALARLADALAAAGGGAPAEIARALRAALPAAVAELADAQRHLAESAARRDALVATAADELRA
AARLLEPDGGPARAEQVRRLLELADELDRARSPEAAEGHAAPRRRGERPLLLVADDEPDAREVLSQVLAPEYEVASASDG
QEALDLARAEHPDLVLLDIGMPRLDGFEVLQRLRQDPATADIPVIFVSGRGDDAVKVRSLDLGAVDYLQKPFSERELRAR
VERTLRLVRSQIALRELAQTDALTGLANLRAFRARVDEEVKRARRYRTPLTCVMADMDNLKPINDALGHAAGDRAIAAVA
AVIREELRETDFGARYGGDEFVLLLPHTTGEEGHVFAERVCTRLHETVLEVSGERVPLGASFGVACLPDEDGDDGGAEAL
VRAADAALYVAKRAGRGRVALAATEPHASASAG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=206, Percent_Identity=35.9223300970874, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=35.8823529411765, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=35.0282485875706, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788381, Length=185, Percent_Identity=34.0540540540541, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=34.1040462427746, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786599, Length=135, Percent_Identity=34.8148148148148, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081974, Length=178, Percent_Identity=29.2134831460674, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=36.9230769230769, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788394, Length=135, Percent_Identity=36.2962962962963, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=30.4093567251462, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=35.0877192982456, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=41.025641025641, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790860, Length=126, Percent_Identity=36.5079365079365, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790863, Length=126, Percent_Identity=32.5396825396825, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=40.1709401709402, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786784, Length=133, Percent_Identity=33.8345864661654, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=31.2849162011173, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693157, Length=105, Percent_Identity=37.1428571428571, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788192, Length=108, Percent_Identity=36.1111111111111, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=122, Percent_Identity=35.2459016393443, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI2367329, Length=117, Percent_Identity=42.7350427350427, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=35.042735042735, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI87081977, Length=242, Percent_Identity=27.6859504132231, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46129; Mature: 45998

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVNGHPEGPPSDVPASDALARLADALAAAGGGAPAEIARALRAALPAAVAELADAQRH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAESAARRDALVATAADELRAAARLLEPDGGPARAEQVRRLLELADELDRARSPEAAEGH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AAPRRRGERPLLLVADDEPDAREVLSQVLAPEYEVASASDGQEALDLARAEHPDLVLLDI
CCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC
GMPRLDGFEVLQRLRQDPATADIPVIFVSGRGDDAVKVRSLDLGAVDYLQKPFSERELRA
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHCCCHHHHHHH
RVERTLRLVRSQIALRELAQTDALTGLANLRAFRARVDEEVKRARRYRTPLTCVMADMDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
LKPINDALGHAAGDRAIAAVAAVIREELRETDFGARYGGDEFVLLLPHTTGEEGHVFAER
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHH
VCTRLHETVLEVSGERVPLGASFGVACLPDEDGDDGGAEALVRAADAALYVAKRAGRGRV
HHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
ALAATEPHASASAG
EEEECCCCCCCCCC
>Mature Secondary Structure 
SSVNGHPEGPPSDVPASDALARLADALAAAGGGAPAEIARALRAALPAAVAELADAQRH
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAESAARRDALVATAADELRAAARLLEPDGGPARAEQVRRLLELADELDRARSPEAAEGH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AAPRRRGERPLLLVADDEPDAREVLSQVLAPEYEVASASDGQEALDLARAEHPDLVLLDI
CCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC
GMPRLDGFEVLQRLRQDPATADIPVIFVSGRGDDAVKVRSLDLGAVDYLQKPFSERELRA
CCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHCCCHHHHHHH
RVERTLRLVRSQIALRELAQTDALTGLANLRAFRARVDEEVKRARRYRTPLTCVMADMDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
LKPINDALGHAAGDRAIAAVAAVIREELRETDFGARYGGDEFVLLLPHTTGEEGHVFAER
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHHH
VCTRLHETVLEVSGERVPLGASFGVACLPDEDGDDGGAEALVRAADAALYVAKRAGRGRV
HHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
ALAATEPHASASAG
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA