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The map label for this gene is pyrB

Identifier: 220917430

GI number: 220917430

Start: 2581744

End: 2582685

Strand: Reverse

Name: pyrB

Synonym: A2cp1_2330

Alternate gene names: 220917430

Gene position: 2582685-2581744 (Counterclockwise)

Preceding gene: 220917431

Following gene: 220917429

Centisome position: 51.35

GC content: 71.02

Gene sequence:

>942_bases
GTGATCGGTCGACACAAGCACTGCATCGCGCTGGAGGACTTCTCCCGCGAGGAGATCCTCGAGGTCATCGACCTCGCCGC
CTCCATGAAGGAGGTCCTGCAGCGGCCGATCAAGAAGGTCCCGAGCCTGCGCGGCAAGATGGTGGTGAACCTCTTCTTCG
AGGCCTCCACCCGCACCCGCAGCTCGTTCGAGACCGCCGCCAAGATCCTGTCCGCCGACGCGCTCAACTGGACGTCCTCC
TCCTCGTCGGTCACCAAGGGCGAGACGCTGGTGGACACGGCGAAGAACCTCGAGGCGATGCGCCCGGACGTGCTGGTGAT
CCGGCACTCGGCCGGCGGCGCGCCGCGGCTGGTCGCGGAGCACGTGGGCTGCTCGGTGGTCTCCGCCGGCGACGGCGCGC
ACGAGCACCCGAGCCAGGGGCTCCTCGACTGCTTCACGCTGCGGGAGAAGCTCGGCACGCTCGAGGGCAAGACCGTCGCC
ATCGTGGGCGACGTCAGCCACTCGCGCGTGGCCCGCTCCGACCTGCACGCGTTCCCGAAGCTGGGGGCGAAGGTCCGGCT
GTGCGGGCCGCCCACCATGATGCCGGCCGGGGTGGAGCGGCTCGGCGCCACGGTCCACACCGATCTGCGCGAGGCGGTGG
ACGGCGCGGACGCGGTCATCATGCTGCGCATCCAGCACGAGCGCATCGGCGACCCGCTCATCCCCGGCACGCGCGAGTAC
TCGAAGGTCTGGGGCCTGAACGCGAAGAAGGCGGCGGACTGGCTGAAGCCCTCCTGCGTGATCCTGCACCCCGGCCCCAT
CAACCGCGGCGTGGAGCTGTCGCCCGAGGTGGCGGACGGGCCCCGCTCGGTGATCCTGGACCAGGTGCAGAACGGGGTGG
CGGTCCGGATGGCGATCCTGTACCTGCTCGCGGGCGGCGCGGGCGAGGAGGCCAGGGCGTGA

Upstream 100 bases:

>100_bases
GCGCGCGGCTGGAGGTCTCGGACCGGGACGACGTGCAGGTGCGCCTGGTGGAGTCGGGCGCGCCCGAGGACGCGGTGGTG
GTCAAGCCGAGGAGGGCGCC

Downstream 100 bases:

>100_bases
CGGCGCGGCTCGGGACGGGAGGCAGGCGCGATGTCTGACGTGCTCTTCATCGAGGGCGGCCGGGTCATCGACCCGGCGAG
CGGCGTGGACGGCGTCCGCA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIGRHKHCIALEDFSREEILEVIDLAASMKEVLQRPIKKVPSLRGKMVVNLFFEASTRTRSSFETAAKILSADALNWTSS
SSSVTKGETLVDTAKNLEAMRPDVLVIRHSAGGAPRLVAEHVGCSVVSAGDGAHEHPSQGLLDCFTLREKLGTLEGKTVA
IVGDVSHSRVARSDLHAFPKLGAKVRLCGPPTMMPAGVERLGATVHTDLREAVDGADAVIMLRIQHERIGDPLIPGTREY
SKVWGLNAKKAADWLKPSCVILHPGPINRGVELSPEVADGPRSVILDQVQNGVAVRMAILYLLAGGAGEEARA

Sequences:

>Translated_313_residues
MIGRHKHCIALEDFSREEILEVIDLAASMKEVLQRPIKKVPSLRGKMVVNLFFEASTRTRSSFETAAKILSADALNWTSS
SSSVTKGETLVDTAKNLEAMRPDVLVIRHSAGGAPRLVAEHVGCSVVSAGDGAHEHPSQGLLDCFTLREKLGTLEGKTVA
IVGDVSHSRVARSDLHAFPKLGAKVRLCGPPTMMPAGVERLGATVHTDLREAVDGADAVIMLRIQHERIGDPLIPGTREY
SKVWGLNAKKAADWLKPSCVILHPGPINRGVELSPEVADGPRSVILDQVQNGVAVRMAILYLLAGGAGEEARA
>Mature_313_residues
MIGRHKHCIALEDFSREEILEVIDLAASMKEVLQRPIKKVPSLRGKMVVNLFFEASTRTRSSFETAAKILSADALNWTSS
SSSVTKGETLVDTAKNLEAMRPDVLVIRHSAGGAPRLVAEHVGCSVVSAGDGAHEHPSQGLLDCFTLREKLGTLEGKTVA
IVGDVSHSRVARSDLHAFPKLGAKVRLCGPPTMMPAGVERLGATVHTDLREAVDGADAVIMLRIQHERIGDPLIPGTREY
SKVWGLNAKKAADWLKPSCVILHPGPINRGVELSPEVADGPRSVILDQVQNGVAVRMAILYLLAGGAGEEARA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=34.3042071197411, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=24.2718446601942, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=33.974358974359, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790703, Length=332, Percent_Identity=26.5060240963855, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786469, Length=335, Percent_Identity=27.4626865671642, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=34.5454545454545, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322331, Length=318, Percent_Identity=30.5031446540881, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24642586, Length=322, Percent_Identity=31.9875776397516, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_ANAD2 (B8JAE9)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492734.1
- ProteinModelPortal:   B8JAE9
- GeneID:   7297584
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2330
- HOGENOM:   HBG579429
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33560; Mature: 33560

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRHKHCIALEDFSREEILEVIDLAASMKEVLQRPIKKVPSLRGKMVVNLFFEASTRTR
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCH
SSFETAAKILSADALNWTSSSSSVTKGETLVDTAKNLEAMRPDVLVIRHSAGGAPRLVAE
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
HVGCSVVSAGDGAHEHPSQGLLDCFTLREKLGTLEGKTVAIVGDVSHSRVARSDLHAFPK
HCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
LGAKVRLCGPPTMMPAGVERLGATVHTDLREAVDGADAVIMLRIQHERIGDPLIPGTREY
CCCEEEECCCCCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEHHHCCCCCCCCCHHH
SKVWGLNAKKAADWLKPSCVILHPGPINRGVELSPEVADGPRSVILDQVQNGVAVRMAIL
HHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
YLLAGGAGEEARA
HHHCCCCCCCCCC
>Mature Secondary Structure
MIGRHKHCIALEDFSREEILEVIDLAASMKEVLQRPIKKVPSLRGKMVVNLFFEASTRTR
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCH
SSFETAAKILSADALNWTSSSSSVTKGETLVDTAKNLEAMRPDVLVIRHSAGGAPRLVAE
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
HVGCSVVSAGDGAHEHPSQGLLDCFTLREKLGTLEGKTVAIVGDVSHSRVARSDLHAFPK
HCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
LGAKVRLCGPPTMMPAGVERLGATVHTDLREAVDGADAVIMLRIQHERIGDPLIPGTREY
CCCEEEECCCCCCCHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEHHHCCCCCCCCCHHH
SKVWGLNAKKAADWLKPSCVILHPGPINRGVELSPEVADGPRSVILDQVQNGVAVRMAIL
HHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
YLLAGGAGEEARA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA