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The map label for this gene is fixL [H]

Identifier: 220917425

GI number: 220917425

Start: 2576764

End: 2577909

Strand: Reverse

Name: fixL [H]

Synonym: A2cp1_2325

Alternate gene names: 220917425

Gene position: 2577909-2576764 (Counterclockwise)

Preceding gene: 220917427

Following gene: 220917424

Centisome position: 51.26

GC content: 75.31

Gene sequence:

>1146_bases
GTGGACTTCTCCCTGCTCGACTCGGTGCCGGACGCCATGGTCATCGCCGACGACGCCGGCGCGATCGTCTTCGTGAACGC
GCACGCCGAGCGCCTCTTCGGCTACCGGTGCCAGGACCTGGTGGGCAGGCCGGTGGAGCTGCTGCTCCCGGCGCGCTACC
GGACCATGCACCAGGTGCACCGGACGGCGTACCAGGCGGCGCCGCGCACGCGCCCCATGGGGCTCGGCCTCGATCTCTCC
GGGCTGCGTCACGACGGGGCCGAGTTCCCGGCCGAGATCAGCCTGTCGCCGATCCTGGTGGACGGGCGCCCCTGCGTGAT
CGCGGCCGTGCGCGACGCGACCGAGCGGCGGAAGATCGAGGAGCGCGCGCGGCTGTGGCGGCGGGCGCAGGAGGAGGTGC
GCGAGCGCGACGAGTTCCTGTCGGTGGCCTCGCACGAGCTGCGCACCCCGGTGACCGCGCTCCAGCTCCAGCTCCAGCTG
CTCCACCGCGCCGCGCTCCGCTCGGGCGAGGGCCTGCCGGCGGTGGTGGTGGAGCGGCTGGAGACGCTCGAGCGGCAGAC
GCGCCGCCTGGGCGCGCTGGTCGGAGAGCTGCTCGACGTCTCGCGCATGCGCCTCGGCAAGATCGAGCTCCGGCGCGAGC
CGCTCGATCTGGCCGAGGTGGCGCGCGAGGCGGCGGGCCACGCGGTGGGCGATCTGGAGCGCTCCGGGTCGAAGCTCGCG
CTCGACCTGCAGCCGGTGACCGGCGCCTGGGACCGCACCCGGCTCGAGCAGGTGATCGCGAACCTGCTGGTGAACGCCGC
CAAGTTCGGGCAGGGGCGGCCCATCGCGCTGCACGTCGCCGGCGACGACGGCACCGCGCGGCTGCGCGTCAGCGACCAGG
GCATCGGCATCGCGCAGGACCAGCAGGCGCGGGTGTTCGACCGCTTCGCGCGCGGCGTGCCGGCCCAGAACTTCGGCGGG
CTCGGGCTGGGGCTCTACATCGCGCGGCAGATCGTGGAGGCGCACGGCGGCACCATCGACGTGACGAGCGAGCCCGGCGC
CGGCGCGACGTTCACCGTGGACCTGCCGCGCCAGCCGCCGGCGCCCGGTCCCGAGGCCCGCCCGGACGCCGGCGCGAAGG
AGCCGCAGCCCGGCGACCTCCATTGA

Upstream 100 bases:

>100_bases
GCCGGCCCGCGGGGGCGGGGCGGGCGGGCGAGCGAGGGCTCGCACCGGGAGGGCGCGGGACTTCACGGAGCGCGCCGGAC
GCCGCATCTCAGAGGGCGGC

Downstream 100 bases:

>100_bases
GCGCGATCGCGGCGGCGCCGCGCTTGACTCCTTGGACCGGGCGCGCGACGGTCGCGGGCGTCGCCATGCGCTGGCTGATC
GTGAACGCCGACGACCTCGG

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MDFSLLDSVPDAMVIADDAGAIVFVNAHAERLFGYRCQDLVGRPVELLLPARYRTMHQVHRTAYQAAPRTRPMGLGLDLS
GLRHDGAEFPAEISLSPILVDGRPCVIAAVRDATERRKIEERARLWRRAQEEVRERDEFLSVASHELRTPVTALQLQLQL
LHRAALRSGEGLPAVVVERLETLERQTRRLGALVGELLDVSRMRLGKIELRREPLDLAEVAREAAGHAVGDLERSGSKLA
LDLQPVTGAWDRTRLEQVIANLLVNAAKFGQGRPIALHVAGDDGTARLRVSDQGIGIAQDQQARVFDRFARGVPAQNFGG
LGLGLYIARQIVEAHGGTIDVTSEPGAGATFTVDLPRQPPAPGPEARPDAGAKEPQPGDLH

Sequences:

>Translated_381_residues
MDFSLLDSVPDAMVIADDAGAIVFVNAHAERLFGYRCQDLVGRPVELLLPARYRTMHQVHRTAYQAAPRTRPMGLGLDLS
GLRHDGAEFPAEISLSPILVDGRPCVIAAVRDATERRKIEERARLWRRAQEEVRERDEFLSVASHELRTPVTALQLQLQL
LHRAALRSGEGLPAVVVERLETLERQTRRLGALVGELLDVSRMRLGKIELRREPLDLAEVAREAAGHAVGDLERSGSKLA
LDLQPVTGAWDRTRLEQVIANLLVNAAKFGQGRPIALHVAGDDGTARLRVSDQGIGIAQDQQARVFDRFARGVPAQNFGG
LGLGLYIARQIVEAHGGTIDVTSEPGAGATFTVDLPRQPPAPGPEARPDAGAKEPQPGDLH
>Mature_381_residues
MDFSLLDSVPDAMVIADDAGAIVFVNAHAERLFGYRCQDLVGRPVELLLPARYRTMHQVHRTAYQAAPRTRPMGLGLDLS
GLRHDGAEFPAEISLSPILVDGRPCVIAAVRDATERRKIEERARLWRRAQEEVRERDEFLSVASHELRTPVTALQLQLQL
LHRAALRSGEGLPAVVVERLETLERQTRRLGALVGELLDVSRMRLGKIELRREPLDLAEVAREAAGHAVGDLERSGSKLA
LDLQPVTGAWDRTRLEQVIANLLVNAAKFGQGRPIALHVAGDDGTARLRVSDQGIGIAQDQQARVFDRFARGVPAQNFGG
LGLGLYIARQIVEAHGGTIDVTSEPGAGATFTVDLPRQPPAPGPEARPDAGAKEPQPGDLH

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=257, Percent_Identity=33.4630350194552, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=36.4341085271318, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789149, Length=280, Percent_Identity=30.3571428571429, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI48994928, Length=251, Percent_Identity=31.0756972111554, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788713, Length=262, Percent_Identity=27.4809160305344, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786600, Length=360, Percent_Identity=29.1666666666667, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788393, Length=225, Percent_Identity=32.4444444444444, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788549, Length=374, Percent_Identity=25.9358288770053, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790346, Length=224, Percent_Identity=30.8035714285714, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87081816, Length=246, Percent_Identity=29.6747967479675, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790436, Length=251, Percent_Identity=28.2868525896414, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786783, Length=236, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789808, Length=228, Percent_Identity=27.1929824561404, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787894, Length=233, Percent_Identity=27.4678111587983, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41528; Mature: 41528

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSLLDSVPDAMVIADDAGAIVFVNAHAERLFGYRCQDLVGRPVELLLPARYRTMHQVH
CCCHHHHCCCCCEEEECCCCEEEEEECCHHHHHCCHHHHHCCCCHHHHCCHHHHHHHHHH
RTAYQAAPRTRPMGLGLDLSGLRHDGAEFPAEISLSPILVDGRPCVIAAVRDATERRKIE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEHHHHHHHHHHH
ERARLWRRAQEEVRERDEFLSVASHELRTPVTALQLQLQLLHRAALRSGEGLPAVVVERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ETLERQTRRLGALVGELLDVSRMRLGKIELRREPLDLAEVAREAAGHAVGDLERSGSKLA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCHHHHHHCCCCEEE
LDLQPVTGAWDRTRLEQVIANLLVNAAKFGQGRPIALHVAGDDGTARLRVSDQGIGIAQD
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCC
QQARVFDRFARGVPAQNFGGLGLGLYIARQIVEAHGGTIDVTSEPGAGATFTVDLPRQPP
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCC
APGPEARPDAGAKEPQPGDLH
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDFSLLDSVPDAMVIADDAGAIVFVNAHAERLFGYRCQDLVGRPVELLLPARYRTMHQVH
CCCHHHHCCCCCEEEECCCCEEEEEECCHHHHHCCHHHHHCCCCHHHHCCHHHHHHHHHH
RTAYQAAPRTRPMGLGLDLSGLRHDGAEFPAEISLSPILVDGRPCVIAAVRDATERRKIE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEHHHHHHHHHHH
ERARLWRRAQEEVRERDEFLSVASHELRTPVTALQLQLQLLHRAALRSGEGLPAVVVERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ETLERQTRRLGALVGELLDVSRMRLGKIELRREPLDLAEVAREAAGHAVGDLERSGSKLA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCHHHHHHCCCCEEE
LDLQPVTGAWDRTRLEQVIANLLVNAAKFGQGRPIALHVAGDDGTARLRVSDQGIGIAQD
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCC
QQARVFDRFARGVPAQNFGGLGLGLYIARQIVEAHGGTIDVTSEPGAGATFTVDLPRQPP
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCCCC
APGPEARPDAGAKEPQPGDLH
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]