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The map label for this gene is rlmN [H]

Identifier: 220917417

GI number: 220917417

Start: 2568070

End: 2569188

Strand: Reverse

Name: rlmN [H]

Synonym: A2cp1_2317

Alternate gene names: 220917417

Gene position: 2569188-2568070 (Counterclockwise)

Preceding gene: 220917418

Following gene: 220917416

Centisome position: 51.08

GC content: 73.1

Gene sequence:

>1119_bases
ATGGACGTCCTGCACGACGACACGCCCGACCTCCGGTCGCTCCCGCAGGAGCGGCTCGCCGCCCTGATCGCCGGCCTGGG
CGAGAAGCCGTTCCGCGCCCGCCAGGTCTACCGCTGGCTGCACCTGCGCGGCGCCGCCTCGCTCGAGGAGCTGACCGACG
TACCGCGCGCGCTGCGCGAGCGGCTGGCGGAGGGCACCCGCCTCACCACGCTCGAGCGCGCCACCGAGCAGCGCTCGGCC
GACGGCACCATCAAGTGGACCTGGCGCACGGGCGACGGGAAGCTGATCGAGTCCGTCTACATGCCCGAGACCGATCGCAA
GACGCTGTGCGTCTCGACGCAGGTGGGCTGCGCGGTGGGCTGCACCTTCTGCATGACCGGCACCATGGGCCTCGCGCGCA
ACCTCACGCCGGGCGAGATCGTGGATCAGGTCCACCGCGCCAACCGCCGGCTGATCGAGCTGGGGGAGGGGGAGGGGCCC
CGGCCGCTCACCAACCTCGTGTTCATGGGCATGGGCGAGCCGCTCGCGAACTACCGCAGCCTGAAGGTCGCGCTCGACCT
GCTCCTGTCCGAGGACGGTCCCAACTTCTCGCACCGCCACGTGACGGTCTCGACGTCCGGGCTGGTGCCGGTGATGCGCC
GGCTGGGCGAGGAGACGCAGGTGAAGCTGGCCGTGTCGCTGAACGCCACCACCGACGCGCAGCGCGACGCGATCATGCCC
ATCAACCGGCGCTACCCGCTCGCCGAGCTGCTCCGCGCCTGCCGCGAGTTCCCGATGAAGCAGGGGCGGCGGATCACGTT
CGAGTACGTGATGCTGGGCGGCGTGAACGACGCGCCGGAGGACGCCGAGCGCCTGGCGCGGCTGCTGCGCGGCATCCCGG
CCAAGGTGAACCTCATCCCGTACAACGAGAACCCGGGCCTCGGGTTCGCCGCCCCCGCGCCGTCCGCGGTGGAGCGGTTC
CGCGACCTGCTCGTCGCCCGGAACGTGACCGCGGTGGTCCGCAAGAACCGCGGCACCGACATCGCCGCCGCCTGCGGCCA
GCTCGCCGCGGAGGGTGGACCGGGCGATCCGCGCCGGCGGGCCGCTGCGCCGTTGACCGGGACGCCCGCGGCGGGCTAG

Upstream 100 bases:

>100_bases
CGACGAGCGGCGCGTCACCCGGGTCGAGATCGCGCGGCGGGGCGAGGTGGCCGCACCCGAGGTCGCGCCGGCCGCGGCCC
GGTCTTAGGAACAGGCGCGC

Downstream 100 bases:

>100_bases
GTACGGGTCGCCCGCCGCTCCCGGCGGGCGGAAAGGCTCGATCCCGGATGGCCCTGCTCGTCGTCGGCTCCGTCGCGCTC
GACTCGCTCGAGACCCCCTT

Product: radical SAM enzyme, Cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MDVLHDDTPDLRSLPQERLAALIAGLGEKPFRARQVYRWLHLRGAASLEELTDVPRALRERLAEGTRLTTLERATEQRSA
DGTIKWTWRTGDGKLIESVYMPETDRKTLCVSTQVGCAVGCTFCMTGTMGLARNLTPGEIVDQVHRANRRLIELGEGEGP
RPLTNLVFMGMGEPLANYRSLKVALDLLLSEDGPNFSHRHVTVSTSGLVPVMRRLGEETQVKLAVSLNATTDAQRDAIMP
INRRYPLAELLRACREFPMKQGRRITFEYVMLGGVNDAPEDAERLARLLRGIPAKVNLIPYNENPGLGFAAPAPSAVERF
RDLLVARNVTAVVRKNRGTDIAAACGQLAAEGGPGDPRRRAAAPLTGTPAAG

Sequences:

>Translated_372_residues
MDVLHDDTPDLRSLPQERLAALIAGLGEKPFRARQVYRWLHLRGAASLEELTDVPRALRERLAEGTRLTTLERATEQRSA
DGTIKWTWRTGDGKLIESVYMPETDRKTLCVSTQVGCAVGCTFCMTGTMGLARNLTPGEIVDQVHRANRRLIELGEGEGP
RPLTNLVFMGMGEPLANYRSLKVALDLLLSEDGPNFSHRHVTVSTSGLVPVMRRLGEETQVKLAVSLNATTDAQRDAIMP
INRRYPLAELLRACREFPMKQGRRITFEYVMLGGVNDAPEDAERLARLLRGIPAKVNLIPYNENPGLGFAAPAPSAVERF
RDLLVARNVTAVVRKNRGTDIAAACGQLAAEGGPGDPRRRAAAPLTGTPAAG
>Mature_372_residues
MDVLHDDTPDLRSLPQERLAALIAGLGEKPFRARQVYRWLHLRGAASLEELTDVPRALRERLAEGTRLTTLERATEQRSA
DGTIKWTWRTGDGKLIESVYMPETDRKTLCVSTQVGCAVGCTFCMTGTMGLARNLTPGEIVDQVHRANRRLIELGEGEGP
RPLTNLVFMGMGEPLANYRSLKVALDLLLSEDGPNFSHRHVTVSTSGLVPVMRRLGEETQVKLAVSLNATTDAQRDAIMP
INRRYPLAELLRACREFPMKQGRRITFEYVMLGGVNDAPEDAERLARLLRGIPAKVNLIPYNENPGLGFAAPAPSAVERF
RDLLVARNVTAVVRKNRGTDIAAACGQLAAEGGPGDPRRRAAAPLTGTPAAG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=350, Percent_Identity=45.1428571428571, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40690; Mature: 40690

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLHDDTPDLRSLPQERLAALIAGLGEKPFRARQVYRWLHLRGAASLEELTDVPRALRE
CCCCCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RLAEGTRLTTLERATEQRSADGTIKWTWRTGDGKLIESVYMPETDRKTLCVSTQVGCAVG
HHHCCCEEHHHHHHHHHCCCCCEEEEEEECCCCCEEHHCCCCCCCCCEEEEECCCCHHHH
CTFCMTGTMGLARNLTPGEIVDQVHRANRRLIELGEGEGPRPLTNLVFMGMGEPLANYRS
HHHHHHCCHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHH
LKVALDLLLSEDGPNFSHRHVTVSTSGLVPVMRRLGEETQVKLAVSLNATTDAQRDAIMP
HHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
INRRYPLAELLRACREFPMKQGRRITFEYVMLGGVNDAPEDAERLARLLRGIPAKVNLIP
CCCCCCHHHHHHHHHHCHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEE
YNENPGLGFAAPAPSAVERFRDLLVARNVTAVVRKNRGTDIAAACGQLAAEGGPGDPRRR
ECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHH
AAAPLTGTPAAG
CCCCCCCCCCCC
>Mature Secondary Structure
MDVLHDDTPDLRSLPQERLAALIAGLGEKPFRARQVYRWLHLRGAASLEELTDVPRALRE
CCCCCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RLAEGTRLTTLERATEQRSADGTIKWTWRTGDGKLIESVYMPETDRKTLCVSTQVGCAVG
HHHCCCEEHHHHHHHHHCCCCCEEEEEEECCCCCEEHHCCCCCCCCCEEEEECCCCHHHH
CTFCMTGTMGLARNLTPGEIVDQVHRANRRLIELGEGEGPRPLTNLVFMGMGEPLANYRS
HHHHHHCCHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHH
LKVALDLLLSEDGPNFSHRHVTVSTSGLVPVMRRLGEETQVKLAVSLNATTDAQRDAIMP
HHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
INRRYPLAELLRACREFPMKQGRRITFEYVMLGGVNDAPEDAERLARLLRGIPAKVNLIP
CCCCCCHHHHHHHHHHCHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEE
YNENPGLGFAAPAPSAVERFRDLLVARNVTAVVRKNRGTDIAAACGQLAAEGGPGDPRRR
ECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHH
AAAPLTGTPAAG
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA