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The map label for this gene is cysD [H]

Identifier: 220917302

GI number: 220917302

Start: 2443369

End: 2444295

Strand: Reverse

Name: cysD [H]

Synonym: A2cp1_2202

Alternate gene names: 220917302

Gene position: 2444295-2443369 (Counterclockwise)

Preceding gene: 220917318

Following gene: 220917301

Centisome position: 48.6

GC content: 72.17

Gene sequence:

>927_bases
ATGACGGACATCCATCCAGCAGAGCTCCGGCGCCCCCTGGCGCACCTCGACTGGCTCGAGGCCGAGGCGATCCACGTGCT
GCGCGAGGTGGCCGGGCAGTGCGCGAACCCCGCGCTGCTGTTCTCCGGCGGCAAGGACTCGGTCTGCCTGCTGCGCCTCG
CCGAGAAGGCGTTCCGGCCCGCGCGCTTCCCCTTCCCGCTGCTCCACATCGACACCGGCCACAACTACGCCGAGGTGATC
GCGTTCCGCGACCGCCGCGCCGCGGAGCTCGGCGAGCGGCTGGTGGTGCGCTCGGTGGAGGACTCGATCCGCGAGGGCAA
GGTTTTGCTGGCCCGGCCGGACGAGCCGCGCAACCGGCACCAGTCGGTCACGCTGCTCGACGCGATCGCCGAGCACGGGT
TCGACGCCTGCATCGGCGGGGCGCGCCGCGACGAGGAGAAGGCCCGCGCGAAGGAGCGGGTCTTCTCGTTCCGCGACGAC
CTCGGCCAGTGGGATCCCAAGCACCAGCGGCCCGAGCTGTGGAGCCTCTACAACGCGCGGATCCGCAGGGGCGAGCACAT
CCGCGCGTTCCCCATCTCCAACTGGACCGAGCTCGACGTCTGGCAGTACATCGCGCGCGAGCGGCTGGAGCTGCCGTCGA
TCTACTTCGCGCACCGCCGCCCGGTGGTGCGGCGGGGCGGCGCGCTCGTCCCGGTGACCGAAGTCACGCCGCCGCGCCCC
GGCGAGCGGGCCGAGGTCGTCTCGGTCCGGTTCCGCACCGTCGGCGACATCACCTGCACCGCGCCGGTCGAGTCCACCGC
CGCGACGGTGGAGGAGATCGTCGCCGAGACCGCCACCACCACCGTCACCGAGCGCGGCGCGACGCGCCTCGACGACCAGA
CCTCGGACGCCTCGATGGAGCAGCGCAAGAAGGAGGGGTACTTCTAA

Upstream 100 bases:

>100_bases
CACGCCCGGTTCATAGCACGCGAGCGCCCGGCCGGCGGGCCGCGGACGGCGCGTCCGCTTGACGGGATGTTCGTCCGTTA
ACTAGGATGCGCTTTCCGCT

Downstream 100 bases:

>100_bases
TGTCGCGCGAGGACGGGCTGCTGCGCTTCCTCACCTGCGGGAGCGTGGACGACGGCAAGAGCACGCTCATCGGCCGCCTG
CTGCACGACACCCGCTCGAT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTDIHPAELRRPLAHLDWLEAEAIHVLREVAGQCANPALLFSGGKDSVCLLRLAEKAFRPARFPFPLLHIDTGHNYAEVI
AFRDRRAAELGERLVVRSVEDSIREGKVLLARPDEPRNRHQSVTLLDAIAEHGFDACIGGARRDEEKARAKERVFSFRDD
LGQWDPKHQRPELWSLYNARIRRGEHIRAFPISNWTELDVWQYIARERLELPSIYFAHRRPVVRRGGALVPVTEVTPPRP
GERAEVVSVRFRTVGDITCTAPVESTAATVEEIVAETATTTVTERGATRLDDQTSDASMEQRKKEGYF

Sequences:

>Translated_308_residues
MTDIHPAELRRPLAHLDWLEAEAIHVLREVAGQCANPALLFSGGKDSVCLLRLAEKAFRPARFPFPLLHIDTGHNYAEVI
AFRDRRAAELGERLVVRSVEDSIREGKVLLARPDEPRNRHQSVTLLDAIAEHGFDACIGGARRDEEKARAKERVFSFRDD
LGQWDPKHQRPELWSLYNARIRRGEHIRAFPISNWTELDVWQYIARERLELPSIYFAHRRPVVRRGGALVPVTEVTPPRP
GERAEVVSVRFRTVGDITCTAPVESTAATVEEIVAETATTTVTERGATRLDDQTSDASMEQRKKEGYF
>Mature_307_residues
TDIHPAELRRPLAHLDWLEAEAIHVLREVAGQCANPALLFSGGKDSVCLLRLAEKAFRPARFPFPLLHIDTGHNYAEVIA
FRDRRAAELGERLVVRSVEDSIREGKVLLARPDEPRNRHQSVTLLDAIAEHGFDACIGGARRDEEKARAKERVFSFRDDL
GQWDPKHQRPELWSLYNARIRRGEHIRAFPISNWTELDVWQYIARERLELPSIYFAHRRPVVRRGGALVPVTEVTPPRPG
ERAEVVSVRFRTVGDITCTAPVESTAATVEEIVAETATTTVTERGATRLDDQTSDASMEQRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=298, Percent_Identity=46.6442953020134, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34943; Mature: 34812

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIHPAELRRPLAHLDWLEAEAIHVLREVAGQCANPALLFSGGKDSVCLLRLAEKAFRP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCC
ARFPFPLLHIDTGHNYAEVIAFRDRRAAELGERLVVRSVEDSIREGKVLLARPDEPRNRH
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHH
QSVTLLDAIAEHGFDACIGGARRDEEKARAKERVFSFRDDLGQWDPKHQRPELWSLYNAR
HHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
IRRGEHIRAFPISNWTELDVWQYIARERLELPSIYFAHRRPVVRRGGALVPVTEVTPPRP
HHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCCEEEECCCCCCCC
GERAEVVSVRFRTVGDITCTAPVESTAATVEEIVAETATTTVTERGATRLDDQTSDASME
CCCCCEEEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
QRKKEGYF
HHHHCCCC
>Mature Secondary Structure 
TDIHPAELRRPLAHLDWLEAEAIHVLREVAGQCANPALLFSGGKDSVCLLRLAEKAFRP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCC
ARFPFPLLHIDTGHNYAEVIAFRDRRAAELGERLVVRSVEDSIREGKVLLARPDEPRNRH
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHH
QSVTLLDAIAEHGFDACIGGARRDEEKARAKERVFSFRDDLGQWDPKHQRPELWSLYNAR
HHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
IRRGEHIRAFPISNWTELDVWQYIARERLELPSIYFAHRRPVVRRGGALVPVTEVTPPRP
HHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCCEEEECCCCCCCC
GERAEVVSVRFRTVGDITCTAPVESTAATVEEIVAETATTTVTERGATRLDDQTSDASME
CCCCCEEEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
QRKKEGYF
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA