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The map label for this gene is cysU [H]

Identifier: 220917299

GI number: 220917299

Start: 2440552

End: 2441406

Strand: Reverse

Name: cysU [H]

Synonym: A2cp1_2199

Alternate gene names: 220917299

Gene position: 2441406-2440552 (Counterclockwise)

Preceding gene: 220917300

Following gene: 220917298

Centisome position: 48.54

GC content: 74.85

Gene sequence:

>855_bases
ATGACCGCGGTCGGCGCGGCGACGCTCCCGGGCCGGGCGCGCCGGGTGCTGCCCGGCTTCGGGCTCTCCACCGGGCTCAC
CGTCGCCTGGCTCTCCGCGGTGGTGCTCGTCCCGCTCTCCACGCTGGCGCTGCGCGCGTCCACGCTCTCCTGGGCGCAGG
CCTGGGCCGTCGTGGCCTCGCCGCGCGCGGTCGCCTCCTACCGCCTGAGCTTCGGCGCCTCGCTCGCCGGCGCCGCGGTG
AACGTGGTGTTCGGCGTGCTGGTGGCGTGGGTGCTGGCGCGGTACCGCTTCCCCGGCCGCTCGCTGGTGGACGCGGTGGT
GGACCTTCCGTTCGCGCTGCCCACCGCGGTGAGCGGCATCGCCCTCACGGCGGTGTACGCGCGGAACGGGTGGCTGGGCG
CGCTGCTCGAGCCGCTGGGCGTGAAGGCGACGTTCTCTCCCCTCGGCGTCACGCTGGCGCTCACCTTCATCGGCCTGCCG
TTCGTGATCCGCACCGTCCAGCCGGTGCTGGAGGACCTCGACCCCGAGCTCGAGGAGGCGGCCGCGACGCTCGGCGCGGG
GCGCTGGGCCACCTTCCGCCGGGTCATCCTCCCCGCGCTCGTGCCCGCCGCCGTCACCGGCTTCGCGCTCGCGTTCGCGC
GCGGCCTCGGCGAGTACGGCTCGGTCGTCTTCATCTCGGGGAACATGCCGCTGCGGACCGAGATCGCGCCGCTGCTCATC
ATGACCCGCCTCGAGCAGTACGACTACGCGGGCGCCTCGGCGATCGCGTGCGTGCTGCTGGGCACCTCGTTCGCGCTGCT
CCTCGGGATCAACCGGCTGCAGGCCTGGACCCGCGTGCGCACGGGGACGGCATGA

Upstream 100 bases:

>100_bases
GCCCGGTGCGGCCCGGCGAGGATCCGCGCGCCGGGCGCTGGCGCGGGCGCGAGAAGACCGAGTGCGGGCTGCACCGGCTC
GGGCCGGGCGGGGAGCGGCG

Downstream 100 bases:

>100_bases
GCGCGCGCGTCGCCAACCAGGATCCGGCCGCGGTGCGGTGGGCGCTCACCGGCCTCGCGCTCGCGTTCCTGGCGGCGTTC
GTGCTGGTCCCGGCCGCGGC

Product: sulfate ABC transporter, inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAVGAATLPGRARRVLPGFGLSTGLTVAWLSAVVLVPLSTLALRASTLSWAQAWAVVASPRAVASYRLSFGASLAGAAV
NVVFGVLVAWVLARYRFPGRSLVDAVVDLPFALPTAVSGIALTAVYARNGWLGALLEPLGVKATFSPLGVTLALTFIGLP
FVIRTVQPVLEDLDPELEEAAATLGAGRWATFRRVILPALVPAAVTGFALAFARGLGEYGSVVFISGNMPLRTEIAPLLI
MTRLEQYDYAGASAIACVLLGTSFALLLGINRLQAWTRVRTGTA

Sequences:

>Translated_284_residues
MTAVGAATLPGRARRVLPGFGLSTGLTVAWLSAVVLVPLSTLALRASTLSWAQAWAVVASPRAVASYRLSFGASLAGAAV
NVVFGVLVAWVLARYRFPGRSLVDAVVDLPFALPTAVSGIALTAVYARNGWLGALLEPLGVKATFSPLGVTLALTFIGLP
FVIRTVQPVLEDLDPELEEAAATLGAGRWATFRRVILPALVPAAVTGFALAFARGLGEYGSVVFISGNMPLRTEIAPLLI
MTRLEQYDYAGASAIACVLLGTSFALLLGINRLQAWTRVRTGTA
>Mature_283_residues
TAVGAATLPGRARRVLPGFGLSTGLTVAWLSAVVLVPLSTLALRASTLSWAQAWAVVASPRAVASYRLSFGASLAGAAVN
VVFGVLVAWVLARYRFPGRSLVDAVVDLPFALPTAVSGIALTAVYARNGWLGALLEPLGVKATFSPLGVTLALTFIGLPF
VIRTVQPVLEDLDPELEEAAATLGAGRWATFRRVILPALVPAAVTGFALAFARGLGEYGSVVFISGNMPLRTEIAPLLIM
TRLEQYDYAGASAIACVLLGTSFALLLGINRLQAWTRVRTGTA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=263, Percent_Identity=50.5703422053232, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082099, Length=226, Percent_Identity=34.070796460177, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1786980, Length=192, Percent_Identity=35.9375, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786254, Length=213, Percent_Identity=32.3943661971831, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787369, Length=155, Percent_Identity=30.3225806451613, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787368, Length=199, Percent_Identity=29.1457286432161, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29675; Mature: 29544

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVGAATLPGRARRVLPGFGLSTGLTVAWLSAVVLVPLSTLALRASTLSWAQAWAVVAS
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRAVASYRLSFGASLAGAAVNVVFGVLVAWVLARYRFPGRSLVDAVVDLPFALPTAVSGI
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
ALTAVYARNGWLGALLEPLGVKATFSPLGVTLALTFIGLPFVIRTVQPVLEDLDPELEEA
HHHHHHHCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AATLGAGRWATFRRVILPALVPAAVTGFALAFARGLGEYGSVVFISGNMPLRTEIAPLLI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHH
MTRLEQYDYAGASAIACVLLGTSFALLLGINRLQAWTRVRTGTA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAVGAATLPGRARRVLPGFGLSTGLTVAWLSAVVLVPLSTLALRASTLSWAQAWAVVAS
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRAVASYRLSFGASLAGAAVNVVFGVLVAWVLARYRFPGRSLVDAVVDLPFALPTAVSGI
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
ALTAVYARNGWLGALLEPLGVKATFSPLGVTLALTFIGLPFVIRTVQPVLEDLDPELEEA
HHHHHHHCCCCHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AATLGAGRWATFRRVILPALVPAAVTGFALAFARGLGEYGSVVFISGNMPLRTEIAPLLI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHH
MTRLEQYDYAGASAIACVLLGTSFALLLGINRLQAWTRVRTGTA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]