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The map label for this gene is gdhA [H]

Identifier: 220917267

GI number: 220917267

Start: 2406264

End: 2407790

Strand: Reverse

Name: gdhA [H]

Synonym: A2cp1_2166

Alternate gene names: 220917267

Gene position: 2407790-2406264 (Counterclockwise)

Preceding gene: 220917269

Following gene: 220917264

Centisome position: 47.87

GC content: 67.39

Gene sequence:

>1527_bases
ATGGCCGGACGCAACGGGAAGACGAGCTTCTACGACGAGGCGATGGAGACGTTTCACCGGGCCGCGGACCTCATCAAGAT
GGACCCGCGCGTCCGCATGGAGCTGGAGGAGCCGGACTTCGAGCACATCTTCTACGTCACCGTCGAGACGAGGGACCGGC
TGGTCCCGCTGTCGCCGGAGGAGCAGGCGAAGTTCCGCGATCTCCCCGCGTCTGACGTCAAGGCGGCGGACGCGTTCGAG
CCGCTCGCGAACGGGAACTACGTGCTGCACCGCCGCGCGCTGCTCAAGGCGGACATCACCATGCGCTCGGGCGTGATGAA
CATCCCGGGCAAGGGCTTCTTCCGGATCGAGAAGGGCGGGCCGAAGAAGTTCAAGGCGTACCGGATCCAGCACAACCAGG
TCCGCGGGCCGTACAAGGGCGGCATCCGCTACCACAAGGACGTCTCGCTCGACCTGTTCAAGACGCTCGCGGCCGACATG
ACGTGGAAGACGGCCATCGCCGAGATCCCGTTCGGCGGCGCCAAGGGCGGCATCAAGATCGACCCCTTCAACTACTCGAA
GGAGGAGATCGAGCACCTCACGCTGCGCTTCATCTACAAGATGAAGGGGCTCATCGGGCCCTACATCGACATCCCGGCGC
CGGACGTGAACACCAACGGCGAGATCATGGCGATGATGATGCGCCAGTTCACGGACGGTGAGCGCGAGCACCACCGCCTC
CGCGGCGTGGTCACCGGCAAGGACGTGCGCATCGGCGGCTCGGAGGGGCGCGTCCGCGCCACCGGCCAGGGCGTCGTCTA
CTGCATCGAGGAGTGGGCGCGCGAGCACAAGTTCGACCTGAAGGGCGCGCGCGTCATCGTGCAGGGCTTCGGCAACGTGG
GCTCGAACGCGGCCGAGATCCTCGCCGCCATGGGCGCGAAGATCGTCGCGGTGAACGACGCGTTCGGCACCATCCACGCC
GACGCGGGCCTCGACGTCCATGCGCTGGCCCAGCACGTCCACCAGAACCCGAAGAACCTGAAGAAGACGGTGGCCGGGTT
CACCGGCGGCACCGCCATCTCCAAGGACGACTTCTGGGGCGTGGACGCCGACATCTGCATCCCGGCCGCGCTCGGCGAGG
AGATCGACGGCCCGGTCGCGGAGCGGCTCAAGGTGCGCCTGGTGGCCGAGGCCGCGAACGGCCCGACCACGCGCGAGGGC
GACCGCGTGCTCGCCTCGCGCAAGATCGAGGTCATCCCCGACATCATCTGCAACGCGGGCGGCGTGACCGTCTCGTACTA
CGAGTGGCTGCAGAACCAGCGCCTCGAGCACTGGTCGGAGGCCGAGGTGAACGCCCGCCTCGAGCAGGCCATCAAGAAGA
ACTACGCCATCATCCGCGACATCTCGCTGAACCGCCCGCAGCGCACGCCGCTCTACGACTCGCGGCCGTACTGCGTCGGC
CAGCAGGTGGACGTGCGCACCGCCGCCATGGTGCTCGCGCTGAAGCGCATCGAGGCGCACTACCTGCTGGAAGGCTTCTC
GCAGTAG

Upstream 100 bases:

>100_bases
CTGGCGTTTTCGGGCAAGACGGAACATTGATTAACGGGGAAGCGAAGGCTACCATTCGCCCGCCTTCGGAACTCCCTCAT
GACGCGAGGCAATCACCGAC

Downstream 100 bases:

>100_bases
CGCCCTGCCCCGCCCTGCCCGCTCCCCCGGCGCGTCCGCGGGAGCGGGCGGGTCACTCCTCGTCCCCCGCCACCGCCGCG
AGCAGCGCCTCGCGCCGCGC

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MAGRNGKTSFYDEAMETFHRAADLIKMDPRVRMELEEPDFEHIFYVTVETRDRLVPLSPEEQAKFRDLPASDVKAADAFE
PLANGNYVLHRRALLKADITMRSGVMNIPGKGFFRIEKGGPKKFKAYRIQHNQVRGPYKGGIRYHKDVSLDLFKTLAADM
TWKTAIAEIPFGGAKGGIKIDPFNYSKEEIEHLTLRFIYKMKGLIGPYIDIPAPDVNTNGEIMAMMMRQFTDGEREHHRL
RGVVTGKDVRIGGSEGRVRATGQGVVYCIEEWAREHKFDLKGARVIVQGFGNVGSNAAEILAAMGAKIVAVNDAFGTIHA
DAGLDVHALAQHVHQNPKNLKKTVAGFTGGTAISKDDFWGVDADICIPAALGEEIDGPVAERLKVRLVAEAANGPTTREG
DRVLASRKIEVIPDIICNAGGVTVSYYEWLQNQRLEHWSEAEVNARLEQAIKKNYAIIRDISLNRPQRTPLYDSRPYCVG
QQVDVRTAAMVLALKRIEAHYLLEGFSQ

Sequences:

>Translated_508_residues
MAGRNGKTSFYDEAMETFHRAADLIKMDPRVRMELEEPDFEHIFYVTVETRDRLVPLSPEEQAKFRDLPASDVKAADAFE
PLANGNYVLHRRALLKADITMRSGVMNIPGKGFFRIEKGGPKKFKAYRIQHNQVRGPYKGGIRYHKDVSLDLFKTLAADM
TWKTAIAEIPFGGAKGGIKIDPFNYSKEEIEHLTLRFIYKMKGLIGPYIDIPAPDVNTNGEIMAMMMRQFTDGEREHHRL
RGVVTGKDVRIGGSEGRVRATGQGVVYCIEEWAREHKFDLKGARVIVQGFGNVGSNAAEILAAMGAKIVAVNDAFGTIHA
DAGLDVHALAQHVHQNPKNLKKTVAGFTGGTAISKDDFWGVDADICIPAALGEEIDGPVAERLKVRLVAEAANGPTTREG
DRVLASRKIEVIPDIICNAGGVTVSYYEWLQNQRLEHWSEAEVNARLEQAIKKNYAIIRDISLNRPQRTPLYDSRPYCVG
QQVDVRTAAMVLALKRIEAHYLLEGFSQ
>Mature_507_residues
AGRNGKTSFYDEAMETFHRAADLIKMDPRVRMELEEPDFEHIFYVTVETRDRLVPLSPEEQAKFRDLPASDVKAADAFEP
LANGNYVLHRRALLKADITMRSGVMNIPGKGFFRIEKGGPKKFKAYRIQHNQVRGPYKGGIRYHKDVSLDLFKTLAADMT
WKTAIAEIPFGGAKGGIKIDPFNYSKEEIEHLTLRFIYKMKGLIGPYIDIPAPDVNTNGEIMAMMMRQFTDGEREHHRLR
GVVTGKDVRIGGSEGRVRATGQGVVYCIEEWAREHKFDLKGARVIVQGFGNVGSNAAEILAAMGAKIVAVNDAFGTIHAD
AGLDVHALAQHVHQNPKNLKKTVAGFTGGTAISKDDFWGVDADICIPAALGEEIDGPVAERLKVRLVAEAANGPTTREGD
RVLASRKIEVIPDIICNAGGVTVSYYEWLQNQRLEHWSEAEVNARLEQAIKKNYAIIRDISLNRPQRTPLYDSRPYCVGQ
QVDVRTAAMVLALKRIEAHYLLEGFSQ

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=364, Percent_Identity=39.2857142857143, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI4885281, Length=364, Percent_Identity=39.010989010989, Blast_Score=234, Evalue=2e-61,
Organism=Escherichia coli, GI1788059, Length=332, Percent_Identity=32.8313253012048, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17544676, Length=446, Percent_Identity=32.9596412556054, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324951, Length=364, Percent_Identity=28.8461538461538, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6319256, Length=356, Percent_Identity=29.4943820224719, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45553475, Length=366, Percent_Identity=38.2513661202186, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI45549226, Length=360, Percent_Identity=38.0555555555556, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24649283, Length=369, Percent_Identity=37.1273712737127, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24649460, Length=314, Percent_Identity=37.5796178343949, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 56587; Mature: 56456

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRNGKTSFYDEAMETFHRAADLIKMDPRVRMELEEPDFEHIFYVTVETRDRLVPLSPE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCCEECCCCC
EQAKFRDLPASDVKAADAFEPLANGNYVLHRRALLKADITMRSGVMNIPGKGFFRIEKGG
HHHHHCCCCCHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCEECCCCCEEEEECCC
PKKFKAYRIQHNQVRGPYKGGIRYHKDVSLDLFKTLAADMTWKTAIAEIPFGGAKGGIKI
CCCEEEEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEE
DPFNYSKEEIEHLTLRFIYKMKGLIGPYIDIPAPDVNTNGEIMAMMMRQFTDGEREHHRL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
RGVVTGKDVRIGGSEGRVRATGQGVVYCIEEWAREHKFDLKGARVIVQGFGNVGSNAAEI
HEEEECCEEEECCCCCEEEECCCCEEEEHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHH
LAAMGAKIVAVNDAFGTIHADAGLDVHALAQHVHQNPKNLKKTVAGFTGGTAISKDDFWG
HHHCCCEEEEEECCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCC
VDADICIPAALGEEIDGPVAERLKVRLVAEAANGPTTREGDRVLASRKIEVIPDIICNAG
CCCCEEECHHHCCCCCCCHHHHHEEEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHCCC
GVTVSYYEWLQNQRLEHWSEAEVNARLEQAIKKNYAIIRDISLNRPQRTPLYDSRPYCVG
CCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCC
QQVDVRTAAMVLALKRIEAHYLLEGFSQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGRNGKTSFYDEAMETFHRAADLIKMDPRVRMELEEPDFEHIFYVTVETRDRLVPLSPE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCCEECCCCC
EQAKFRDLPASDVKAADAFEPLANGNYVLHRRALLKADITMRSGVMNIPGKGFFRIEKGG
HHHHHCCCCCHHCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCEECCCCCEEEEECCC
PKKFKAYRIQHNQVRGPYKGGIRYHKDVSLDLFKTLAADMTWKTAIAEIPFGGAKGGIKI
CCCEEEEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEE
DPFNYSKEEIEHLTLRFIYKMKGLIGPYIDIPAPDVNTNGEIMAMMMRQFTDGEREHHRL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
RGVVTGKDVRIGGSEGRVRATGQGVVYCIEEWAREHKFDLKGARVIVQGFGNVGSNAAEI
HEEEECCEEEECCCCCEEEECCCCEEEEHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHH
LAAMGAKIVAVNDAFGTIHADAGLDVHALAQHVHQNPKNLKKTVAGFTGGTAISKDDFWG
HHHCCCEEEEEECCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCC
VDADICIPAALGEEIDGPVAERLKVRLVAEAANGPTTREGDRVLASRKIEVIPDIICNAG
CCCCEEECHHHCCCCCCCHHHHHEEEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHCCC
GVTVSYYEWLQNQRLEHWSEAEVNARLEQAIKKNYAIIRDISLNRPQRTPLYDSRPYCVG
CCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCC
QQVDVRTAAMVLALKRIEAHYLLEGFSQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]