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The map label for this gene is grpE [H]

Identifier: 220917262

GI number: 220917262

Start: 2400156

End: 2400794

Strand: Direct

Name: grpE [H]

Synonym: A2cp1_2160

Alternate gene names: 220917262

Gene position: 2400156-2400794 (Clockwise)

Preceding gene: 220917261

Following gene: 220917263

Centisome position: 47.72

GC content: 77.15

Gene sequence:

>639_bases
ATGGCAGACGAACGCCGCGACACCTCTGCGCCGGGCGCGGAGGGTCAGGTGAAGGTGGTGGATCGACGCCGGTTCCGGCA
GGACGGGGAGCCCCTCGACCCGGCCGGCGCCGACGCGCCGCCGGAGGAGGCGGGTGGGCCGCCCCCGGGCGCGGCCGTCG
AGGCGCGCCTCGCCGAGCAGGCGGCCCGCATCGACGAGCTGACCCGCGCGTACGCCGCCCTGGTCGAGGACAACAGGGCA
TTCCGGCAGCGCCTCGAGCGCGAGCGGACCCGCGTCGTGGACGCCGAGCGGGCCGCGGTGGCGCAGACGCTGCTCGAGGC
CACCGACGACCTGGAGCGCGCCCTCGCGGCCGCGTCCGCGCCCGGTGAGCCCACCGACGAGCGCCTGGCGAACCTGCTGG
AGGGCGTGCGTCTTTCACTGAGCGTGCTGCACCGGCGCATCGCCGCGCTCGGGGCGGAGCGGATCCCGACGCTCGGCCAG
CCGTTCGACCCGCACGTGGCCGAGGCGGTGGACACGGTGCCGGTCGGCGACGCGTCGCAGGACGGCATGGTGGTGCAGGA
GATCCGCGCCGGGTATCGCGTCGGCGACCGCGTGCTCCGCCCCGCCAGGGTCCGCGTGGGGAAGGTGGCGCGCGCGTGA

Upstream 100 bases:

>100_bases
ACCGCCCTGAGGGCGGGCGCCGCAGGCTCGGACGCCCCCGGGGCCTTGCTCGTTTGACACGACCTTTCCGCTGGTTAGCT
TGCCTGCCGGAGGTCGTGAC

Downstream 100 bases:

>100_bases
TGCCGCGCCCCACGCCGTGCCCGCCGGGCGCCGGCGCGGGGAATAGAATCCGGGCCCGAGCGTCCTTGGAACGTGACTGA
CCGTCTCTACATGACCAACC

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MADERRDTSAPGAEGQVKVVDRRRFRQDGEPLDPAGADAPPEEAGGPPPGAAVEARLAEQAARIDELTRAYAALVEDNRA
FRQRLERERTRVVDAERAAVAQTLLEATDDLERALAAASAPGEPTDERLANLLEGVRLSLSVLHRRIAALGAERIPTLGQ
PFDPHVAEAVDTVPVGDASQDGMVVQEIRAGYRVGDRVLRPARVRVGKVARA

Sequences:

>Translated_212_residues
MADERRDTSAPGAEGQVKVVDRRRFRQDGEPLDPAGADAPPEEAGGPPPGAAVEARLAEQAARIDELTRAYAALVEDNRA
FRQRLERERTRVVDAERAAVAQTLLEATDDLERALAAASAPGEPTDERLANLLEGVRLSLSVLHRRIAALGAERIPTLGQ
PFDPHVAEAVDTVPVGDASQDGMVVQEIRAGYRVGDRVLRPARVRVGKVARA
>Mature_211_residues
ADERRDTSAPGAEGQVKVVDRRRFRQDGEPLDPAGADAPPEEAGGPPPGAAVEARLAEQAARIDELTRAYAALVEDNRAF
RQRLERERTRVVDAERAAVAQTLLEATDDLERALAAASAPGEPTDERLANLLEGVRLSLSVLHRRIAALGAERIPTLGQP
FDPHVAEAVDTVPVGDASQDGMVVQEIRAGYRVGDRVLRPARVRVGKVARA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI40255109, Length=180, Percent_Identity=28.8888888888889, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=202, Percent_Identity=27.2277227722772, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22787; Mature: 22656

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADERRDTSAPGAEGQVKVVDRRRFRQDGEPLDPAGADAPPEEAGGPPPGAAVEARLAEQ
CCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHH
AARIDELTRAYAALVEDNRAFRQRLERERTRVVDAERAAVAQTLLEATDDLERALAAASA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGEPTDERLANLLEGVRLSLSVLHRRIAALGAERIPTLGQPFDPHVAEAVDTVPVGDASQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCC
DGMVVQEIRAGYRVGDRVLRPARVRVGKVARA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADERRDTSAPGAEGQVKVVDRRRFRQDGEPLDPAGADAPPEEAGGPPPGAAVEARLAEQ
CCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHH
AARIDELTRAYAALVEDNRAFRQRLERERTRVVDAERAAVAQTLLEATDDLERALAAASA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGEPTDERLANLLEGVRLSLSVLHRRIAALGAERIPTLGQPFDPHVAEAVDTVPVGDASQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCC
DGMVVQEIRAGYRVGDRVLRPARVRVGKVARA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500908 [H]