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The map label for this gene is yitJ [H]

Identifier: 220917256

GI number: 220917256

Start: 2393547

End: 2394407

Strand: Reverse

Name: yitJ [H]

Synonym: A2cp1_2154

Alternate gene names: 220917256

Gene position: 2394407-2393547 (Counterclockwise)

Preceding gene: 220917257

Following gene: 220917253

Centisome position: 47.61

GC content: 83.51

Gene sequence:

>861_bases
ATGACGCCCGCCTCGCCGCGCCCGTTCGACGGCCCCACCCTGCTCGACGGGGCCATGGGCACCGCGCTCCTCGCCGGCGG
CCTCCCCGCCGGCACGCTGCCCGAGGCCTGGGTGCTGGAGCGGCCGGAGGCGGTCGCCGCGGTCCACGCCGCCCACGCGG
CGGCGGGCGCCGAGGTGGTGCTCACCTGCACGTTCAACGCGGCCGGTCCGCGCCTCGACCCGCTCGTCCCGCCGGACCGG
GTCGGCGCGCTCTGCGCCGCCGCGGTGCGCCTCGCGCGCCGGGCCGCCCCCGGCGCGCGCGTGGCCGGCGACCTCGGGCC
CACCGCGCTGTACGGCCCCGGACGCCCGCCCCCCGACGGCGCGGCGGTGCGCGCCCGGTACGCGCGGGCCGCCGGCGCGC
TCGCGGCGGCCGGCGCGGATCTGCTGTGGGCGGAGTCGCAGTGGGACCTCGCCGAGGCGCGGCTCGCGCTCGACGCCGCG
CGCGCCACCGGCCTTCCGGTGGTGGTGACGTTCGCGCTGCGCGAGGCGGCCGGCCGGCTCGCGGCGCCGGACGGCACGCC
GGCCGAGGAGCTCCTGGAGGCGGTCGCGGACGCCGGCGCCGCGGCGGCGGGGGTGAACTGCGTGCCGCCGGGACCGGCGC
TCGCCGCGCTCGCCGCCTGGGCCGCGCGGCGGCTGGCGGTGCGGTTCGTGGCGAAGCCAAGCCCCGGGCTGCCCGGCGAG
GTGCTCGGGCCGGACGCGTTCGCGGAGGCGCTCCGCCCCGCCGTCCATGCGGGCCTGGGGGTCGCCGGAGGCTGCTGCGG
CGCCGGGCCCGGGCACCTCGCGGCGCTCCGGCGGCTGCTCGCGGCCGCCCGGGCCGGCTAG

Upstream 100 bases:

>100_bases
CCGACGAGCTCATCGCGCTCGGCCACGCCGACGCGCGGCAGCGCCGCGACGCCCTGCTCGCCTTCTTCGCCGAGCCCGTC
GCCGCCGGCGCGCGCACCGC

Downstream 100 bases:

>100_bases
ACCGGCGCGCCGGTGCGCGGGTACGAGCCCAGGAGGTGCAGCTCGTGCACCTGCTCGCGCACGCCCTCGAGCGCGCTCGC
GCCGGCGGGGTCCGCGCGGT

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTPASPRPFDGPTLLDGAMGTALLAGGLPAGTLPEAWVLERPEAVAAVHAAHAAAGAEVVLTCTFNAAGPRLDPLVPPDR
VGALCAAAVRLARRAAPGARVAGDLGPTALYGPGRPPPDGAAVRARYARAAGALAAAGADLLWAESQWDLAEARLALDAA
RATGLPVVVTFALREAAGRLAAPDGTPAEELLEAVADAGAAAAGVNCVPPGPALAALAAWAARRLAVRFVAKPSPGLPGE
VLGPDAFAEALRPAVHAGLGVAGGCCGAGPGHLAALRRLLAAARAG

Sequences:

>Translated_286_residues
MTPASPRPFDGPTLLDGAMGTALLAGGLPAGTLPEAWVLERPEAVAAVHAAHAAAGAEVVLTCTFNAAGPRLDPLVPPDR
VGALCAAAVRLARRAAPGARVAGDLGPTALYGPGRPPPDGAAVRARYARAAGALAAAGADLLWAESQWDLAEARLALDAA
RATGLPVVVTFALREAAGRLAAPDGTPAEELLEAVADAGAAAAGVNCVPPGPALAALAAWAARRLAVRFVAKPSPGLPGE
VLGPDAFAEALRPAVHAGLGVAGGCCGAGPGHLAALRRLLAAARAG
>Mature_285_residues
TPASPRPFDGPTLLDGAMGTALLAGGLPAGTLPEAWVLERPEAVAAVHAAHAAAGAEVVLTCTFNAAGPRLDPLVPPDRV
GALCAAAVRLARRAAPGARVAGDLGPTALYGPGRPPPDGAAVRARYARAAGALAAAGADLLWAESQWDLAEARLALDAAR
ATGLPVVVTFALREAAGRLAAPDGTPAEELLEAVADAGAAAAGVNCVPPGPALAALAAWAARRLAVRFVAKPSPGLPGEV
LGPDAFAEALRPAVHAGLGVAGGCCGAGPGHLAALRRLLAAARAG

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790450, Length=308, Percent_Identity=28.8961038961039, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535405, Length=313, Percent_Identity=25.5591054313099, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 28029; Mature: 27898

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPASPRPFDGPTLLDGAMGTALLAGGLPAGTLPEAWVLERPEAVAAVHAAHAAAGAEVV
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEE
LTCTFNAAGPRLDPLVPPDRVGALCAAAVRLARRAAPGARVAGDLGPTALYGPGRPPPDG
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
AAVRARYARAAGALAAAGADLLWAESQWDLAEARLALDAARATGLPVVVTFALREAAGRL
HHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
AAPDGTPAEELLEAVADAGAAAAGVNCVPPGPALAALAAWAARRLAVRFVAKPSPGLPGE
CCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
VLGPDAFAEALRPAVHAGLGVAGGCCGAGPGHLAALRRLLAAARAG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPASPRPFDGPTLLDGAMGTALLAGGLPAGTLPEAWVLERPEAVAAVHAAHAAAGAEVV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEE
LTCTFNAAGPRLDPLVPPDRVGALCAAAVRLARRAAPGARVAGDLGPTALYGPGRPPPDG
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
AAVRARYARAAGALAAAGADLLWAESQWDLAEARLALDAARATGLPVVVTFALREAAGRL
HHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
AAPDGTPAEELLEAVADAGAAAAGVNCVPPGPALAALAAWAARRLAVRFVAKPSPGLPGE
CCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
VLGPDAFAEALRPAVHAGLGVAGGCCGAGPGHLAALRRLLAAARAG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]