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The map label for this gene is slt [H]

Identifier: 220917161

GI number: 220917161

Start: 2298161

End: 2300413

Strand: Direct

Name: slt [H]

Synonym: A2cp1_2058

Alternate gene names: 220917161

Gene position: 2298161-2300413 (Clockwise)

Preceding gene: 220917158

Following gene: 220917162

Centisome position: 45.7

GC content: 79.54

Gene sequence:

>2253_bases
ATGCTCACCGCCGCGCTCACCGCCTGCCTCCTCGCCGCCGCCCCGACCCCCACGCCCCCGCCGCTCCGCGAGGCCGACCT
CGCGCCGCTGTTCCGCAAGGGCCCCGCCGCGGAGGGAAAGGCCGCCTACGACGCCGGGCGCCACGCCGACGCCGCCGCGC
GGCTCGCGAGGGCGCCCGAGCCGGAGGCGGCCTACATCCGCGCGCTGGCGCTCCTGGAGGTCCCGCGGCCCGAGGAGGCG
CTGAAGGTCCTCGCCGGCCTCGAGGAGAAGGTGCCCGACCTCGCCGACCGCGTGGCGTTCCTGCGCGGCGCGGCGCTGTC
CGCGGCGGGACGCCCGCGCGAGGCGATGGCGGCGTGGGCGCAGGTGCCGGACCGGTCGCTGCTCGCCGCCGAGGCGCGGC
TCTCGCGGGCGCGCGCCGCGTCGGCGCTCGGCGAGGGCGCGGCCGCGCTCGAGGCGCTCGCGCCGCTGCTGCGCGACGGC
GCGCCGGACGATCTCTCCCGGCCCGACCTCGCCGCCTCCGCGCTGCTCCTCGCCGGCCGGATCCGCGGCGCAGCGCGGCC
CCCCGATCCCGCCGGCGCGCGGCGCGACCTGATGGCGTGCTGGACGGCGCACCCGCTCGCGCCGGAGTCCTCGGAGTGCC
TCTCCGCGGCGGCCGCGCTGCCGGCGCCGCACGGCGGCCCGCCCTCGGCCGAGGAGACGCTGCGGCGCGCGGAGGGGCTG
CTCGACGCGAACCGCAACGGGCAGGCGCTGGCGCTGCTCGAGCCCGCGGTGGCCGCGCTCCGCCCCGACGACCTCGGCGC
CGCCGCGTGCCGCGCCCGGGCGGCGCTGGGCCGCGGCTACCGCAAGGAGCGCAGCTACCGGCAGGCGATCGACACGCTGC
GGCCGGTGGTGGCGGGCTGCGAGGACGAGGGGCTGCGGGTCCGCTCGCTGTACCTGCTCGCCGGCGCGTCGTCCATCTCC
GGGGATCGCGACGAGGCGGTGGCGCTGTACCGCCAGCTGGCGCGCGACTTCGCCGGCCACGCGTTCGCCGACGACGCGCT
GTTCTTCGCCGCAGACCTCCTGGCGCGCGCCGGCAAGTCGCAGGAGGCCCGCGAGGCGCTCGCGGCGCTGGTGCGGGATC
ACCCGGGCGGCGACTACCGCGAGGAGGCGCGCTTCCGGCTCGCGTGGCTGCTGAAGCAGGCGGGCGACCTGGATGGCGCG
GTCGCGCAGCTCCTCGCGGTCGAGGAGGAGCAGGCGGGCCGCGACGGCTACGAGCACGCCCGGGCGGCCTACTGGCGCGC
GCGGCTGCTGGCCGGGCGCGGCGAGGACGGGCGCCGGGCCGCCGAGGCGGTGTTCACCGAGCTCGCCACGCGCTACCCCA
CCGACTACTACGGCCTGCTCGCCCGGGCCCGGCTCGACGGCCACGGCGGCCAGAGCCTCCCCGCGCGGCGGGCCGCGCCG
GCCGGCGGCACCCCCGAGGCCGCCTACGACCCCGGCCCGCTGCGCGAGGAGCCGCACTTCCGCGCCGGCCTGCTGCTCCT
GCGCATGGGCCTGCCGCGCGCCGCGTCGGAGGAGCTGTCGGCGATCCCGGCCGCGCGGCTGCGCGCGGCCGGCGACGCGC
CCGAGCCGGTGCTGCTGGTGGCCGACCTGCTCGACCGCGCCGGCGACCACCGCGCCGCGCACAACCTGCTGCGCACCCGC
GCCCGCGGCGCGCTCCGCAAGCCGCCCGAGGGCGAGAACCTGCGCGCCTGGCGCATCGCGTACCCGCCGGCCTACCGCGA
CGACGTGCGCCGCTGGGCGCCGCCCGCGGGCGTGCCGGTGGAGCTGGTGCAGGCGCTCATGCGCGAGGAGAGCGCGCTCG
ACCCGCGCGCCATGTCCGGCGCCGGCGCGGTCGGCCTCACCCAGCTCATGCTGCCCACCGCGCAGTCGGTGGCGAAGAGC
CTGAAGCTCTCCCGCCCGACGCGCACCGACCTCATGCGCGCGTCGCTCAACATCCGACTCGGCTCGCACTACCTGGGCGA
GCTGGTGCGCCGGTTCGACGGCTCGCTCCCGCTCGCGCTCGCAGCGTACAACGCCGGCGGCGGGGCGGTCCGGCGCTGGC
TGGGCGGGCGCGACGCGCTCGACGTGGACGAGTTCGTGGAGGAGATCCCGGTGGAGGAGACGCGCGGCTACGTGAAGCGC
GTGCTCCGGTCCTACGCCGCGTACCGGCTGCTCTACGGCGCGCCCGAGGAGGCGTCCCTCGGGCTGCTCCGCCGCGCCGG
GGGCAAGGGCTGA

Upstream 100 bases:

>100_bases
TTCCTCCCGGTGCGCCCGGGCGGCACGCCCCCGCTCGCCCCTGCGGGCGAACGGTCCCTTGACCGCGTCTCCCCCGGGTC
CCACTCTGTCCCCCGGACCG

Downstream 100 bases:

>100_bases
CCCGGAGGAGGGGCGCACCGCGACCCACCCGACCCGCGGCGATCCGCGCCCCCGGGCCCGCGTGCGGCCAGCGGGCCGGC
CGCGTATGATCTTCCGAACA

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MLTAALTACLLAAAPTPTPPPLREADLAPLFRKGPAAEGKAAYDAGRHADAAARLARAPEPEAAYIRALALLEVPRPEEA
LKVLAGLEEKVPDLADRVAFLRGAALSAAGRPREAMAAWAQVPDRSLLAAEARLSRARAASALGEGAAALEALAPLLRDG
APDDLSRPDLAASALLLAGRIRGAARPPDPAGARRDLMACWTAHPLAPESSECLSAAAALPAPHGGPPSAEETLRRAEGL
LDANRNGQALALLEPAVAALRPDDLGAAACRARAALGRGYRKERSYRQAIDTLRPVVAGCEDEGLRVRSLYLLAGASSIS
GDRDEAVALYRQLARDFAGHAFADDALFFAADLLARAGKSQEAREALAALVRDHPGGDYREEARFRLAWLLKQAGDLDGA
VAQLLAVEEEQAGRDGYEHARAAYWRARLLAGRGEDGRRAAEAVFTELATRYPTDYYGLLARARLDGHGGQSLPARRAAP
AGGTPEAAYDPGPLREEPHFRAGLLLLRMGLPRAASEELSAIPAARLRAAGDAPEPVLLVADLLDRAGDHRAAHNLLRTR
ARGALRKPPEGENLRAWRIAYPPAYRDDVRRWAPPAGVPVELVQALMREESALDPRAMSGAGAVGLTQLMLPTAQSVAKS
LKLSRPTRTDLMRASLNIRLGSHYLGELVRRFDGSLPLALAAYNAGGGAVRRWLGGRDALDVDEFVEEIPVEETRGYVKR
VLRSYAAYRLLYGAPEEASLGLLRRAGGKG

Sequences:

>Translated_750_residues
MLTAALTACLLAAAPTPTPPPLREADLAPLFRKGPAAEGKAAYDAGRHADAAARLARAPEPEAAYIRALALLEVPRPEEA
LKVLAGLEEKVPDLADRVAFLRGAALSAAGRPREAMAAWAQVPDRSLLAAEARLSRARAASALGEGAAALEALAPLLRDG
APDDLSRPDLAASALLLAGRIRGAARPPDPAGARRDLMACWTAHPLAPESSECLSAAAALPAPHGGPPSAEETLRRAEGL
LDANRNGQALALLEPAVAALRPDDLGAAACRARAALGRGYRKERSYRQAIDTLRPVVAGCEDEGLRVRSLYLLAGASSIS
GDRDEAVALYRQLARDFAGHAFADDALFFAADLLARAGKSQEAREALAALVRDHPGGDYREEARFRLAWLLKQAGDLDGA
VAQLLAVEEEQAGRDGYEHARAAYWRARLLAGRGEDGRRAAEAVFTELATRYPTDYYGLLARARLDGHGGQSLPARRAAP
AGGTPEAAYDPGPLREEPHFRAGLLLLRMGLPRAASEELSAIPAARLRAAGDAPEPVLLVADLLDRAGDHRAAHNLLRTR
ARGALRKPPEGENLRAWRIAYPPAYRDDVRRWAPPAGVPVELVQALMREESALDPRAMSGAGAVGLTQLMLPTAQSVAKS
LKLSRPTRTDLMRASLNIRLGSHYLGELVRRFDGSLPLALAAYNAGGGAVRRWLGGRDALDVDEFVEEIPVEETRGYVKR
VLRSYAAYRLLYGAPEEASLGLLRRAGGKG
>Mature_750_residues
MLTAALTACLLAAAPTPTPPPLREADLAPLFRKGPAAEGKAAYDAGRHADAAARLARAPEPEAAYIRALALLEVPRPEEA
LKVLAGLEEKVPDLADRVAFLRGAALSAAGRPREAMAAWAQVPDRSLLAAEARLSRARAASALGEGAAALEALAPLLRDG
APDDLSRPDLAASALLLAGRIRGAARPPDPAGARRDLMACWTAHPLAPESSECLSAAAALPAPHGGPPSAEETLRRAEGL
LDANRNGQALALLEPAVAALRPDDLGAAACRARAALGRGYRKERSYRQAIDTLRPVVAGCEDEGLRVRSLYLLAGASSIS
GDRDEAVALYRQLARDFAGHAFADDALFFAADLLARAGKSQEAREALAALVRDHPGGDYREEARFRLAWLLKQAGDLDGA
VAQLLAVEEEQAGRDGYEHARAAYWRARLLAGRGEDGRRAAEAVFTELATRYPTDYYGLLARARLDGHGGQSLPARRAAP
AGGTPEAAYDPGPLREEPHFRAGLLLLRMGLPRAASEELSAIPAARLRAAGDAPEPVLLVADLLDRAGDHRAAHNLLRTR
ARGALRKPPEGENLRAWRIAYPPAYRDDVRRWAPPAGVPVELVQALMREESALDPRAMSGAGAVGLTQLMLPTAQSVAKS
LKLSRPTRTDLMRASLNIRLGSHYLGELVRRFDGSLPLALAAYNAGGGAVRRWLGGRDALDVDEFVEEIPVEETRGYVKR
VLRSYAAYRLLYGAPEEASLGLLRRAGGKG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=157, Percent_Identity=39.4904458598726, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79736; Mature: 79736

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAALTACLLAAAPTPTPPPLREADLAPLFRKGPAAEGKAAYDAGRHADAAARLARAPE
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCHHHHHHHHCCCC
PEAAYIRALALLEVPRPEEALKVLAGLEEKVPDLADRVAFLRGAALSAAGRPREAMAAWA
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QVPDRSLLAAEARLSRARAASALGEGAAALEALAPLLRDGAPDDLSRPDLAASALLLAGR
HCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IRGAARPPDPAGARRDLMACWTAHPLAPESSECLSAAAALPAPHGGPPSAEETLRRAEGL
HCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LDANRNGQALALLEPAVAALRPDDLGAAACRARAALGRGYRKERSYRQAIDTLRPVVAGC
HCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
EDEGLRVRSLYLLAGASSISGDRDEAVALYRQLARDFAGHAFADDALFFAADLLARAGKS
CCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
QEAREALAALVRDHPGGDYREEARFRLAWLLKQAGDLDGAVAQLLAVEEEQAGRDGYEHA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH
RAAYWRARLLAGRGEDGRRAAEAVFTELATRYPTDYYGLLARARLDGHGGQSLPARRAAP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCC
AGGTPEAAYDPGPLREEPHFRAGLLLLRMGLPRAASEELSAIPAARLRAAGDAPEPVLLV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHCCCCCCHHHHH
ADLLDRAGDHRAAHNLLRTRARGALRKPPEGENLRAWRIAYPPAYRDDVRRWAPPAGVPV
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHH
ELVQALMREESALDPRAMSGAGAVGLTQLMLPTAQSVAKSLKLSRPTRTDLMRASLNIRL
HHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEE
GSHYLGELVRRFDGSLPLALAAYNAGGGAVRRWLGGRDALDVDEFVEEIPVEETRGYVKR
CHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHH
VLRSYAAYRLLYGAPEEASLGLLRRAGGKG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTAALTACLLAAAPTPTPPPLREADLAPLFRKGPAAEGKAAYDAGRHADAAARLARAPE
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCHHHHHHHHCCCC
PEAAYIRALALLEVPRPEEALKVLAGLEEKVPDLADRVAFLRGAALSAAGRPREAMAAWA
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QVPDRSLLAAEARLSRARAASALGEGAAALEALAPLLRDGAPDDLSRPDLAASALLLAGR
HCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IRGAARPPDPAGARRDLMACWTAHPLAPESSECLSAAAALPAPHGGPPSAEETLRRAEGL
HCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LDANRNGQALALLEPAVAALRPDDLGAAACRARAALGRGYRKERSYRQAIDTLRPVVAGC
HCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
EDEGLRVRSLYLLAGASSISGDRDEAVALYRQLARDFAGHAFADDALFFAADLLARAGKS
CCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
QEAREALAALVRDHPGGDYREEARFRLAWLLKQAGDLDGAVAQLLAVEEEQAGRDGYEHA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH
RAAYWRARLLAGRGEDGRRAAEAVFTELATRYPTDYYGLLARARLDGHGGQSLPARRAAP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCC
AGGTPEAAYDPGPLREEPHFRAGLLLLRMGLPRAASEELSAIPAARLRAAGDAPEPVLLV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHCCCCCCHHHHH
ADLLDRAGDHRAAHNLLRTRARGALRKPPEGENLRAWRIAYPPAYRDDVRRWAPPAGVPV
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHH
ELVQALMREESALDPRAMSGAGAVGLTQLMLPTAQSVAKSLKLSRPTRTDLMRASLNIRL
HHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEE
GSHYLGELVRRFDGSLPLALAAYNAGGGAVRRWLGGRDALDVDEFVEEIPVEETRGYVKR
CHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHH
VLRSYAAYRLLYGAPEEASLGLLRRAGGKG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]