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The map label for this gene is rpoA

Identifier: 220917148

GI number: 220917148

Start: 2286382

End: 2287404

Strand: Direct

Name: rpoA

Synonym: A2cp1_2045

Alternate gene names: 220917148

Gene position: 2286382-2287404 (Clockwise)

Preceding gene: 220917147

Following gene: 220917149

Centisome position: 45.46

GC content: 65.98

Gene sequence:

>1023_bases
ATGGTCGATCCGATCGTCACGAAGAACTGGCGGGACCTCATCAAGCCCCGCGGCCTGGTCGTCGACCAGGACAGCCTCTC
GAACACCTACGGGAAGTTCGTCGCGGAGCCGCTCGAGCGGGGCTTCGGCATCACCCTCGGCAACTCGCTCCGGCGCGTGC
TGCTCTCGAGCCTGCAGGGCGCCGCGATCACCTCGGTGAAGGTCGAGGGCGTCGAGCACGAGTTCATGACCATCCCCGAG
GTGGCCGAGGACGTCACCGACATCATCCTCAACCTCAAGGAAGTGCTGCTCCAGATCCACACCAACGACGTGAAGACGAT
CCGGATCGAGGCGGACGGCCCGAAGGAGATCAAGGCCGGCGACCTCATCACGGACGCGCAGGTCGAGGTGCTGAACCCGG
GCCACCACATCCTCACCATCAGCGAGGGCGGGCGGGTCCGCGCCGAGATGACCGCGCGCCGCGGCCGCGGCTACGTGCCG
GCCGAGCGGAACAAGATCCCCGGCTCGCCGATCGGCACCATCCCCATCGACGCGCTCTTCAGCCCCATCCGCAAGGTGAA
CTACCAGGTCACCAACGCGCGCGTCGGGCAGCAGACCGACTACGACAAGCTGACGCTCGAGGTCTGGACCGACGGCTCGG
TCGCCCCGGCCGACGCGGTCGCCTTCGCCGCGAAGATCGTGAAGGAGCAGCTCTCGATCTTCATCAACTTCGACGAGGCG
GAGGAGCCGGCCGAGGAGATCAAGCCGGTCGAGGAGCAGAAGCTGAACGAGAACCTCTTCCGCTCGGTGGACGAGCTGGA
GCTCTCGGTCCGCAGCGCGAACTGCCTGCAGAACGCGAACATCAAGACCATCGGCGACCTCGTTCAGAAGACCGAGGCCG
AGATGCTCAAGACGAAGAATTTCGGCCGGAAGTCGCTGAAGGAGATCAAGGAGATCCTGGCCGAGATGGGCCTCTCCCTC
GGCATGAAGCTCGAGAACTGGCCGCCGAAGGCGGCGCCGCAGGGCGGCGCGCCCAAGGTCTAG

Upstream 100 bases:

>100_bases
GGCCCGCACCGCACCATCCCAAGCAGTCGCGCGTCCCACGGTGCTCCCGCCGCATGGCTGAGCGGGCCGGGAGAGGGGAC
GGCGGCCGAGGAGACAGCCC

Downstream 100 bases:

>100_bases
CGCGCCCGCACAGGGACAGGAGATCACCAGATGAAGCACCGCGTCGTCGGCCGCCGGCTCGATCGCACCACCGAGCACCG
GACCGCAATGTTCAAGAACA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MVDPIVTKNWRDLIKPRGLVVDQDSLSNTYGKFVAEPLERGFGITLGNSLRRVLLSSLQGAAITSVKVEGVEHEFMTIPE
VAEDVTDIILNLKEVLLQIHTNDVKTIRIEADGPKEIKAGDLITDAQVEVLNPGHHILTISEGGRVRAEMTARRGRGYVP
AERNKIPGSPIGTIPIDALFSPIRKVNYQVTNARVGQQTDYDKLTLEVWTDGSVAPADAVAFAAKIVKEQLSIFINFDEA
EEPAEEIKPVEEQKLNENLFRSVDELELSVRSANCLQNANIKTIGDLVQKTEAEMLKTKNFGRKSLKEIKEILAEMGLSL
GMKLENWPPKAAPQGGAPKV

Sequences:

>Translated_340_residues
MVDPIVTKNWRDLIKPRGLVVDQDSLSNTYGKFVAEPLERGFGITLGNSLRRVLLSSLQGAAITSVKVEGVEHEFMTIPE
VAEDVTDIILNLKEVLLQIHTNDVKTIRIEADGPKEIKAGDLITDAQVEVLNPGHHILTISEGGRVRAEMTARRGRGYVP
AERNKIPGSPIGTIPIDALFSPIRKVNYQVTNARVGQQTDYDKLTLEVWTDGSVAPADAVAFAAKIVKEQLSIFINFDEA
EEPAEEIKPVEEQKLNENLFRSVDELELSVRSANCLQNANIKTIGDLVQKTEAEMLKTKNFGRKSLKEIKEILAEMGLSL
GMKLENWPPKAAPQGGAPKV
>Mature_340_residues
MVDPIVTKNWRDLIKPRGLVVDQDSLSNTYGKFVAEPLERGFGITLGNSLRRVLLSSLQGAAITSVKVEGVEHEFMTIPE
VAEDVTDIILNLKEVLLQIHTNDVKTIRIEADGPKEIKAGDLITDAQVEVLNPGHHILTISEGGRVRAEMTARRGRGYVP
AERNKIPGSPIGTIPIDALFSPIRKVNYQVTNARVGQQTDYDKLTLEVWTDGSVAPADAVAFAAKIVKEQLSIFINFDEA
EEPAEEIKPVEEQKLNENLFRSVDELELSVRSANCLQNANIKTIGDLVQKTEAEMLKTKNFGRKSLKEIKEILAEMGLSL
GMKLENWPPKAAPQGGAPKV

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=324, Percent_Identity=45.0617283950617, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_ANAD2 (B8J887)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002492452.1
- GeneID:   7300172
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_2045
- HOGENOM:   HBG430844
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37435; Mature: 37435

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDPIVTKNWRDLIKPRGLVVDQDSLSNTYGKFVAEPLERGFGITLGNSLRRVLLSSLQG
CCCCCCCCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCC
AAITSVKVEGVEHEFMTIPEVAEDVTDIILNLKEVLLQIHTNDVKTIRIEADGPKEIKAG
CEEEEEEECCCCCHHEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
DLITDAQVEVLNPGHHILTISEGGRVRAEMTARRGRGYVPAERNKIPGSPIGTIPIDALF
CCEECCEEEEECCCCEEEEECCCCEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
SPIRKVNYQVTNARVGQQTDYDKLTLEVWTDGSVAPADAVAFAAKIVKEQLSIFINFDEA
HHHHHHCEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEECCCC
EEPAEEIKPVEEQKLNENLFRSVDELELSVRSANCLQNANIKTIGDLVQKTEAEMLKTKN
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
FGRKSLKEIKEILAEMGLSLGMKLENWPPKAAPQGGAPKV
CCHHHHHHHHHHHHHCCHHCCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVDPIVTKNWRDLIKPRGLVVDQDSLSNTYGKFVAEPLERGFGITLGNSLRRVLLSSLQG
CCCCCCCCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCC
AAITSVKVEGVEHEFMTIPEVAEDVTDIILNLKEVLLQIHTNDVKTIRIEADGPKEIKAG
CEEEEEEECCCCCHHEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
DLITDAQVEVLNPGHHILTISEGGRVRAEMTARRGRGYVPAERNKIPGSPIGTIPIDALF
CCEECCEEEEECCCCEEEEECCCCEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
SPIRKVNYQVTNARVGQQTDYDKLTLEVWTDGSVAPADAVAFAAKIVKEQLSIFINFDEA
HHHHHHCEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEECCCC
EEPAEEIKPVEEQKLNENLFRSVDELELSVRSANCLQNANIKTIGDLVQKTEAEMLKTKN
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
FGRKSLKEIKEILAEMGLSLGMKLENWPPKAAPQGGAPKV
CCHHHHHHHHHHHHHCCHHCCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA