Click here to switch to the map view.

The map label for this gene is purU [H]

Identifier: 220917055

GI number: 220917055

Start: 2204869

End: 2205729

Strand: Reverse

Name: purU [H]

Synonym: A2cp1_1951

Alternate gene names: 220917055

Gene position: 2205729-2204869 (Counterclockwise)

Preceding gene: 220917071

Following gene: 220917054

Centisome position: 43.86

GC content: 70.03

Gene sequence:

>861_bases
GTGACCCAGCCCCGCGCCATCCTCCTCGTCCAGTGCCCCGACCGCCCCGGCATCGTGGCGGCCATCTCCAGCTTCCTCTA
CCGCCACGGCGCGAACATCCTCGACTTCGACCAGCACACCTCGGTGGACAACGGCGGCGCCTACTTCACCCGCCTCGAGT
TCCAGACCGACCGGCTGGACCTCCCGGTGGAGGACCTGGAGCGCGCGTTCGCCCTCGACGTCGCCAGGCCGTTCGGCATG
GAGTGGCGGCTCACCCTCTCCTCGCAGCGCAAGAAGGTCGCGATCCTGGTCTCGAAGCACGACCACGCGCTCCTGGAGCT
GCTGTGGAACTGGGATCGCGGCGACCTGCACGCGGACGTCTCCACGGTGATCTCCAACCACCCCGACCTGCGCGAGTCGG
TGGAGTCGTTCGGCGTCCCGTTCGTCCACGTGCCCAACAGCCGCGACACCCGCGCGCAGGCCGAGGCCCGCATGCTGGAG
CTGCTCGAGGGGAAGGCCGACCTGGTGGTGCTCGCGCGCTACATGCAGATCGTCTCGCCGGAGCTGGTGGCGCGCTGGCC
GAACCGCATCATCAACATCCATCACTCCTTCCTCCCCGCGTTCGTGGGCGCCGACCCGTACCGGCAGGCGTACGACCGCG
GGGTGAAGATCGTCGGCGCCACCGCCCACTACGTCACCGCCGAGCTCGACGCCGGGCCCATCATCGACCAGGACGTGGGC
CGGGTCTCGCACCGCGACGCGGTGGACGACCTGAAGCGGCTCGGGCGCGACCTGGAGCGGCGGGTGCTGGCGCGCGCGGT
GCGCTGGCACTGCGAGGACCGCGTCATCGTGAACGGCAACAAGACCGTCGTGTTCAGCTGA

Upstream 100 bases:

>100_bases
CCTCGCCGGCGGCCCCGGCGCGCCGCCCCCGGCCGCGGGTGCGCCCGGGCGCGCAGGGGATTTGATCCCGGGCACGCCTC
CGTCTACCCTGTCGGGTCCC

Downstream 100 bases:

>100_bases
CGCGCGGCGAGGCGCCCGCCCGGCCGCTGCGCCCGGCCCGTGCCCCGGGCCGCGGCCGGTCACACCTTCCGGCGAGGGAG
GTGCGCCATGGCGGCCCGGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTQPRAILLVQCPDRPGIVAAISSFLYRHGANILDFDQHTSVDNGGAYFTRLEFQTDRLDLPVEDLERAFALDVARPFGM
EWRLTLSSQRKKVAILVSKHDHALLELLWNWDRGDLHADVSTVISNHPDLRESVESFGVPFVHVPNSRDTRAQAEARMLE
LLEGKADLVVLARYMQIVSPELVARWPNRIINIHHSFLPAFVGADPYRQAYDRGVKIVGATAHYVTAELDAGPIIDQDVG
RVSHRDAVDDLKRLGRDLERRVLARAVRWHCEDRVIVNGNKTVVFS

Sequences:

>Translated_286_residues
MTQPRAILLVQCPDRPGIVAAISSFLYRHGANILDFDQHTSVDNGGAYFTRLEFQTDRLDLPVEDLERAFALDVARPFGM
EWRLTLSSQRKKVAILVSKHDHALLELLWNWDRGDLHADVSTVISNHPDLRESVESFGVPFVHVPNSRDTRAQAEARMLE
LLEGKADLVVLARYMQIVSPELVARWPNRIINIHHSFLPAFVGADPYRQAYDRGVKIVGATAHYVTAELDAGPIIDQDVG
RVSHRDAVDDLKRLGRDLERRVLARAVRWHCEDRVIVNGNKTVVFS
>Mature_285_residues
TQPRAILLVQCPDRPGIVAAISSFLYRHGANILDFDQHTSVDNGGAYFTRLEFQTDRLDLPVEDLERAFALDVARPFGME
WRLTLSSQRKKVAILVSKHDHALLELLWNWDRGDLHADVSTVISNHPDLRESVESFGVPFVHVPNSRDTRAQAEARMLEL
LEGKADLVVLARYMQIVSPELVARWPNRIINIHHSFLPAFVGADPYRQAYDRGVKIVGATAHYVTAELDAGPIIDQDVGR
VSHRDAVDDLKRLGRDLERRVLARAVRWHCEDRVIVNGNKTVVFS

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=30.5263157894737, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=30.5263157894737, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=30.5263157894737, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=43.5087719298246, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=27.4611398963731, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=196, Percent_Identity=34.1836734693878, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24582400, Length=207, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32367; Mature: 32236

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPRAILLVQCPDRPGIVAAISSFLYRHGANILDFDQHTSVDNGGAYFTRLEFQTDRLD
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEEECCCCC
LPVEDLERAFALDVARPFGMEWRLTLSSQRKKVAILVSKHDHALLELLWNWDRGDLHADV
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHH
STVISNHPDLRESVESFGVPFVHVPNSRDTRAQAEARMLELLEGKADLVVLARYMQIVSP
HHHHCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCH
ELVARWPNRIINIHHSFLPAFVGADPYRQAYDRGVKIVGATAHYVTAELDAGPIIDQDVG
HHHHHCCHHHEEHHHHHHHHHHCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCHHHH
RVSHRDAVDDLKRLGRDLERRVLARAVRWHCEDRVIVNGNKTVVFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEC
>Mature Secondary Structure 
TQPRAILLVQCPDRPGIVAAISSFLYRHGANILDFDQHTSVDNGGAYFTRLEFQTDRLD
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEEEECCCCC
LPVEDLERAFALDVARPFGMEWRLTLSSQRKKVAILVSKHDHALLELLWNWDRGDLHADV
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHH
STVISNHPDLRESVESFGVPFVHVPNSRDTRAQAEARMLELLEGKADLVVLARYMQIVSP
HHHHCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCH
ELVARWPNRIINIHHSFLPAFVGADPYRQAYDRGVKIVGATAHYVTAELDAGPIIDQDVG
HHHHHCCHHHEEHHHHHHHHHHCCCHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCHHHH
RVSHRDAVDDLKRLGRDLERRVLARAVRWHCEDRVIVNGNKTVVFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]