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The map label for this gene is cls1 [H]

Identifier: 220917004

GI number: 220917004

Start: 2132559

End: 2134019

Strand: Reverse

Name: cls1 [H]

Synonym: A2cp1_1900

Alternate gene names: 220917004

Gene position: 2134019-2132559 (Counterclockwise)

Preceding gene: 220917006

Following gene: 220917002

Centisome position: 42.43

GC content: 75.29

Gene sequence:

>1461_bases
ATGTGGACCCCGGACCTCGCGCTCCTCGACGCGCTGCCCTCGCCGGGCGTGCTCGGCGTCCTCGAGGCGGCCTGGGTGGT
TGCGGCGAGCTGCTGGATCCTGCTGGAGCGCCGCTCCCCCACCGCCACGCTGGCCTGGATCCTGGCGCTCGCCGCCATGC
CGGTGGTGGGCGTGGCGGTGTACCTCGTGGTCGGGCCCCGGCGGCTCGACCGCAAGAAGCTGCGCCTGAACGTGCTGAAG
CGCGCCCGCGGCGCGTACCTCGAGGCGTGGGATCGGGCCACCGCGCGCGATCTCTCCAGCATGGGCCAGCTCGCCCGGCT
CGCGATCCGGCTCCAGGCGCCGCCGCCCGAGACCGCGCGATCCGTCGTCCTCTTCTCGGAGGGCGACGACCACTTCGAGG
CGCTGGAGCGAGACCTCGCCGCCGCCCGGCACCACGTCCACGTCGAGATGTACATCTTCGAGCGCGACCGGACCGGCGCG
CGCGTGCTCGCCGCCCTGGCCGCCGCCGCCCGCCGCGGCGTGCAGGTGCGCCTGCTGGTGGACGACGCGGGGTCGCCGCG
GCTCGGCGCCGCGCTGCTCGCGCCGCTCCGTGAGGCGGGCGGCGAGGTGGAGCGCTTCAACCCGATGCTCGTGTCGCGGC
TGCGCGGGGGCTCGGCGAACTTCCGCACCCACCGGAAGATCGTGGTGTGCGACGGGCGGGTGGGCTTCACCGGCGGGATG
AACGTGTCGGACGACCAGAGCGCCGCGGCGGTGGGCCGGTCCGCCTGGCGGGACACCACGGTGCGCATCGAGGGGGCCGC
GGTCCACGGCCTGCAGCTCACCTTCCTCGAGGACTGGGCCTTCGCCACCGGCAAGACCGGCTCGCCGCTGCCGGCGGGCG
GGCTCCGGCCGTACTTCCCCGACGAGCCGCCCGGGTCGCACGAGGTGCAGATCGTGCCGTCCGGCCCGGACCAGGACACG
TCCGCGGTCGGCCAGCTCCACGTGGCCGCGATCGGCGGCGCGCGCGAGCGCGTGTGGATCACCACCCCGTACTTCGTGCC
CGACGAGGCCCTGCTCGCGGCGCTGACCAGCGCCGCGCTCCGCGGCGTCGACGTGCGCCTGCTGCTGCCGCGCCGCACCG
ACTCGCGGGTGGTGGACGCCGCCTCGCGCACGTACCACGACGCGCTGCTCGCGGCCGGCGCGCGGATCTGGCTCTACGGA
CCGCGCATGCTCCACGCCAAGACCTGCCTCGTGGACCAGGACGTCGGGATGATCGGCACCGCCAACCTCGACAACCGAAG
CCTGCGCCTCAACTTCGAGGTCACGGCCGTCCTGTACGGCGCCCCCGCGGTGCAGGCGCTCGCGGACCTGTTCGAGCGCG
ATCTGGAGGTGGCCCGGCCCAAGACCACGCGCGAGGCCGACGCGCCGTTCCTGTCGCGCCTGGGCGCCAGCGCGGCGCGG
CTGCTGGCACCGCAGCTGTAA

Upstream 100 bases:

>100_bases
CCATGCCGCGGGCGGGTGGGTACCGCGCGGTCGGCCGCGCGGCCGCACCGGCCCGGCGGGCGCCGGCACGCCCGCCCGCT
GCGTGTGTAGAATCGCCAAG

Downstream 100 bases:

>100_bases
CGAGGGCTGCGGCCGCTGCCTCCCCAGCCATGCGGCTGGGGCCGCTCCCCGGAAGCCGCGTCAGGCGCCCGCGGCGCCGC
CGCGGTGCCGCGGGTTGCGC

Product: phospholipase D/Transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MWTPDLALLDALPSPGVLGVLEAAWVVAASCWILLERRSPTATLAWILALAAMPVVGVAVYLVVGPRRLDRKKLRLNVLK
RARGAYLEAWDRATARDLSSMGQLARLAIRLQAPPPETARSVVLFSEGDDHFEALERDLAAARHHVHVEMYIFERDRTGA
RVLAALAAAARRGVQVRLLVDDAGSPRLGAALLAPLREAGGEVERFNPMLVSRLRGGSANFRTHRKIVVCDGRVGFTGGM
NVSDDQSAAAVGRSAWRDTTVRIEGAAVHGLQLTFLEDWAFATGKTGSPLPAGGLRPYFPDEPPGSHEVQIVPSGPDQDT
SAVGQLHVAAIGGARERVWITTPYFVPDEALLAALTSAALRGVDVRLLLPRRTDSRVVDAASRTYHDALLAAGARIWLYG
PRMLHAKTCLVDQDVGMIGTANLDNRSLRLNFEVTAVLYGAPAVQALADLFERDLEVARPKTTREADAPFLSRLGASAAR
LLAPQL

Sequences:

>Translated_486_residues
MWTPDLALLDALPSPGVLGVLEAAWVVAASCWILLERRSPTATLAWILALAAMPVVGVAVYLVVGPRRLDRKKLRLNVLK
RARGAYLEAWDRATARDLSSMGQLARLAIRLQAPPPETARSVVLFSEGDDHFEALERDLAAARHHVHVEMYIFERDRTGA
RVLAALAAAARRGVQVRLLVDDAGSPRLGAALLAPLREAGGEVERFNPMLVSRLRGGSANFRTHRKIVVCDGRVGFTGGM
NVSDDQSAAAVGRSAWRDTTVRIEGAAVHGLQLTFLEDWAFATGKTGSPLPAGGLRPYFPDEPPGSHEVQIVPSGPDQDT
SAVGQLHVAAIGGARERVWITTPYFVPDEALLAALTSAALRGVDVRLLLPRRTDSRVVDAASRTYHDALLAAGARIWLYG
PRMLHAKTCLVDQDVGMIGTANLDNRSLRLNFEVTAVLYGAPAVQALADLFERDLEVARPKTTREADAPFLSRLGASAAR
LLAPQL
>Mature_486_residues
MWTPDLALLDALPSPGVLGVLEAAWVVAASCWILLERRSPTATLAWILALAAMPVVGVAVYLVVGPRRLDRKKLRLNVLK
RARGAYLEAWDRATARDLSSMGQLARLAIRLQAPPPETARSVVLFSEGDDHFEALERDLAAARHHVHVEMYIFERDRTGA
RVLAALAAAARRGVQVRLLVDDAGSPRLGAALLAPLREAGGEVERFNPMLVSRLRGGSANFRTHRKIVVCDGRVGFTGGM
NVSDDQSAAAVGRSAWRDTTVRIEGAAVHGLQLTFLEDWAFATGKTGSPLPAGGLRPYFPDEPPGSHEVQIVPSGPDQDT
SAVGQLHVAAIGGARERVWITTPYFVPDEALLAALTSAALRGVDVRLLLPRRTDSRVVDAASRTYHDALLAAGARIWLYG
PRMLHAKTCLVDQDVGMIGTANLDNRSLRLNFEVTAVLYGAPAVQALADLFERDLEVARPKTTREADAPFLSRLGASAAR
LLAPQL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=465, Percent_Identity=34.1935483870968, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1787007, Length=331, Percent_Identity=30.2114803625378, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87081826, Length=391, Percent_Identity=27.8772378516624, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 52419; Mature: 52419

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTPDLALLDALPSPGVLGVLEAAWVVAASCWILLERRSPTATLAWILALAAMPVVGVAV
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHH
YLVVGPRRLDRKKLRLNVLKRARGAYLEAWDRATARDLSSMGQLARLAIRLQAPPPETAR
HEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
SVVLFSEGDDHFEALERDLAAARHHVHVEMYIFERDRTGARVLAALAAAARRGVQVRLLV
EEEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEE
DDAGSPRLGAALLAPLREAGGEVERFNPMLVSRLRGGSANFRTHRKIVVCDGRVGFTGGM
ECCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCEECCEEEEECCCCCCCCCC
NVSDDQSAAAVGRSAWRDTTVRIEGAAVHGLQLTFLEDWAFATGKTGSPLPAGGLRPYFP
CCCCCCHHHHHHHHHCCCCEEEEECEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
DEPPGSHEVQIVPSGPDQDTSAVGQLHVAAIGGARERVWITTPYFVPDEALLAALTSAAL
CCCCCCCEEEEEECCCCCCCHHHCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHH
RGVDVRLLLPRRTDSRVVDAASRTYHDALLAAGARIWLYGPRMLHAKTCLVDQDVGMIGT
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCEEEEHEEEEEECCCCEEEE
ANLDNRSLRLNFEVTAVLYGAPAVQALADLFERDLEVARPKTTREADAPFLSRLGASAAR
CCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHH
LLAPQL
HHCCCC
>Mature Secondary Structure
MWTPDLALLDALPSPGVLGVLEAAWVVAASCWILLERRSPTATLAWILALAAMPVVGVAV
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHH
YLVVGPRRLDRKKLRLNVLKRARGAYLEAWDRATARDLSSMGQLARLAIRLQAPPPETAR
HEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
SVVLFSEGDDHFEALERDLAAARHHVHVEMYIFERDRTGARVLAALAAAARRGVQVRLLV
EEEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEE
DDAGSPRLGAALLAPLREAGGEVERFNPMLVSRLRGGSANFRTHRKIVVCDGRVGFTGGM
ECCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHCCCCCCCEECCEEEEECCCCCCCCCC
NVSDDQSAAAVGRSAWRDTTVRIEGAAVHGLQLTFLEDWAFATGKTGSPLPAGGLRPYFP
CCCCCCHHHHHHHHHCCCCEEEEECEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
DEPPGSHEVQIVPSGPDQDTSAVGQLHVAAIGGARERVWITTPYFVPDEALLAALTSAAL
CCCCCCCEEEEEECCCCCCCHHHCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHH
RGVDVRLLLPRRTDSRVVDAASRTYHDALLAAGARIWLYGPRMLHAKTCLVDQDVGMIGT
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCEEEEHEEEEEECCCCEEEE
ANLDNRSLRLNFEVTAVLYGAPAVQALADLFERDLEVARPKTTREADAPFLSRLGASAAR
CCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCHHHHH
LLAPQL
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]