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The map label for this gene is tgs1 [H]

Identifier: 220916993

GI number: 220916993

Start: 2120509

End: 2121927

Strand: Reverse

Name: tgs1 [H]

Synonym: A2cp1_1889

Alternate gene names: 220916993

Gene position: 2121927-2120509 (Counterclockwise)

Preceding gene: 220916994

Following gene: 220916987

Centisome position: 42.19

GC content: 79.92

Gene sequence:

>1419_bases
ATGACCCGCGAGCCGATGAGCCGGGTGGACGCCGCCTGGCTGCGCATGGACCGCCCCGAGAACACGGCCGACATCGTGGT
GCTGCTGCGCCTCGCGGGTCGCCTCACCCCGCCGCGGCTGCGCACGCTGCTGGAGCAGCGGCTGCTGCCGCACGAGCGGT
TCCGCGCGCGGGTGGCGGACGCCGGCGCGCTGGGCGGCCCGGCCTGGGAGCCGGATCCGCGCTTCTCCATCGCGCGACAC
CTCAGCGCGCGGCCGCTGCGCGAGGCGCACCCGGAGGCGCTCGAGGCGGCCGTGTCCGAGGCGGCCACGCAGCCGCTCCC
GGCCGGCCGCCCGCCCTGGCGCGCGCTGCTGCTCGAGGGCGCCGAGGAGAGCGCGCTGGTGGTGAAGCTCCACCACTGCA
TGGCGGACGGGGTCGCGCTCGTGTCGGTGCTGCTCGGCCTGTCCGACGAGCACGCCGGCCGCGTCGAGGCGCCGGCGCCG
GACGTGCCCGGAGGCGACCCGCTCCGCCGCGGCGCCCGCGCGCTGCTCCGGGATCCGCTCGCCGCCGCGGCCGCGCTCTG
GAAGATGGCCACGCTCCCCACCGCGACCGGCGCGCTCGCGCCGCCGGCGCTCTCCGGCCGCCGGACCGTCGCGTGGTCGC
GGTCGTGGCCGCTCGAGGACGTGCGCGCCGCCGCCCGGGCCGGCGGGGGCACCGTGAACGACGCGCTGCTCGCGGCGCTC
TCCGGCGCGCTCTCGCGCGCCCTCCGCGCCGGCCCCGTCCCGCCGGGACCGCTGCCCGACGACGTGCGCGCGCTGGTGCC
GGTGAACCTGCGCGCGGCGCCCGCGGCGGGGGTGGCCGGGGCGGGCAACCAGTTCGGCCTCGTGTTCCTCGACCTGCCGG
TCGAACCGCTCGCGCCGGACGAGCGGCTGGAGATCGTGCGCGGCCGCACCGCCGCGCTGAAGCGCAGCCCGGACGCCTGG
GTGGCGCTCGGGGTGCTGGGCGCCCTCGGCTTCGCGCCGCCCGCGCTGGAGCGGCTCGGCACCGCGTTCTTCTCGCGGAA
GGCGTCGCTGGTGGTGACCAACGTCCCCGGCCCGCAGCGGCGGCTCCACCTCGCCGGCCGGCGGGTGGACGAGCTGCTGT
TCTGGGTCCCGCACCCGGCCGCGCTCGGGCTGGGCGTGAGCGTCTTCAGCTACGCCGGCAAGGTGACGATGGGCGTGCGC
GCCGACGCGGCGTTCCCGATCGACCCGCGCGCGCTGGCGGGCGCGCTGGACGAGGAGCTGGCGGCGTTCGCGCCGCGGCC
CCCCGCGCGCCGCGCCCGGCCCCCGGCCGCCGCGGCGGCCCCGGAACAGGACGGCCGGCAGGCGGCCTTCGCGGGCGCGC
CTGCCGGCCGGGGGGTGCGGGTGATCGAGGCGGGGCCGGCTACATCCCCAGCTTCTTGA

Upstream 100 bases:

>100_bases
CGGCCGGCCGGCTGCCCTCCCCTGCGTCACGTCGCCCGGGCTCCCTCCCGGTCCCTCCCTCCGCGCGCCGATCCGCGCAG
GATCCGGCCCAGGAGCCGAC

Downstream 100 bases:

>100_bases
AGAAGTCGTTGCCCTTGTCGTCCACCAGGATGAACGCGGGGAAGTTCTCGACCTCGATCTTCCAGACGGCTTCCATGCCG
AGCTCGGCGAAGTCGATGCA

Product: hypothetical protein

Products: NA

Alternate protein names: TGS1; Probable triacylglycerol synthase tgs1 [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTREPMSRVDAAWLRMDRPENTADIVVLLRLAGRLTPPRLRTLLEQRLLPHERFRARVADAGALGGPAWEPDPRFSIARH
LSARPLREAHPEALEAAVSEAATQPLPAGRPPWRALLLEGAEESALVVKLHHCMADGVALVSVLLGLSDEHAGRVEAPAP
DVPGGDPLRRGARALLRDPLAAAAALWKMATLPTATGALAPPALSGRRTVAWSRSWPLEDVRAAARAGGGTVNDALLAAL
SGALSRALRAGPVPPGPLPDDVRALVPVNLRAAPAAGVAGAGNQFGLVFLDLPVEPLAPDERLEIVRGRTAALKRSPDAW
VALGVLGALGFAPPALERLGTAFFSRKASLVVTNVPGPQRRLHLAGRRVDELLFWVPHPAALGLGVSVFSYAGKVTMGVR
ADAAFPIDPRALAGALDEELAAFAPRPPARRARPPAAAAAPEQDGRQAAFAGAPAGRGVRVIEAGPATSPAS

Sequences:

>Translated_472_residues
MTREPMSRVDAAWLRMDRPENTADIVVLLRLAGRLTPPRLRTLLEQRLLPHERFRARVADAGALGGPAWEPDPRFSIARH
LSARPLREAHPEALEAAVSEAATQPLPAGRPPWRALLLEGAEESALVVKLHHCMADGVALVSVLLGLSDEHAGRVEAPAP
DVPGGDPLRRGARALLRDPLAAAAALWKMATLPTATGALAPPALSGRRTVAWSRSWPLEDVRAAARAGGGTVNDALLAAL
SGALSRALRAGPVPPGPLPDDVRALVPVNLRAAPAAGVAGAGNQFGLVFLDLPVEPLAPDERLEIVRGRTAALKRSPDAW
VALGVLGALGFAPPALERLGTAFFSRKASLVVTNVPGPQRRLHLAGRRVDELLFWVPHPAALGLGVSVFSYAGKVTMGVR
ADAAFPIDPRALAGALDEELAAFAPRPPARRARPPAAAAAPEQDGRQAAFAGAPAGRGVRVIEAGPATSPAS
>Mature_471_residues
TREPMSRVDAAWLRMDRPENTADIVVLLRLAGRLTPPRLRTLLEQRLLPHERFRARVADAGALGGPAWEPDPRFSIARHL
SARPLREAHPEALEAAVSEAATQPLPAGRPPWRALLLEGAEESALVVKLHHCMADGVALVSVLLGLSDEHAGRVEAPAPD
VPGGDPLRRGARALLRDPLAAAAALWKMATLPTATGALAPPALSGRRTVAWSRSWPLEDVRAAARAGGGTVNDALLAALS
GALSRALRAGPVPPGPLPDDVRALVPVNLRAAPAAGVAGAGNQFGLVFLDLPVEPLAPDERLEIVRGRTAALKRSPDAWV
ALGVLGALGFAPPALERLGTAFFSRKASLVVTNVPGPQRRLHLAGRRVDELLFWVPHPAALGLGVSVFSYAGKVTMGVRA
DAAFPIDPRALAGALDEELAAFAPRPPARRARPPAAAAAPEQDGRQAAFAGAPAGRGVRVIEAGPATSPAS

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Prefers C(26:0)-CoA over C(18:1)-CoA. TG synthesis activity increases in M.tuberculosis upon oxygen depletion and NO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 49296; Mature: 49165

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREPMSRVDAAWLRMDRPENTADIVVLLRLAGRLTPPRLRTLLEQRLLPHERFRARVAD
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHC
AGALGGPAWEPDPRFSIARHLSARPLREAHPEALEAAVSEAATQPLPAGRPPWRALLLEG
CCCCCCCCCCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
AEESALVVKLHHCMADGVALVSVLLGLSDEHAGRVEAPAPDVPGGDPLRRGARALLRDPL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAAAALWKMATLPTATGALAPPALSGRRTVAWSRSWPLEDVRAAARAGGGTVNDALLAAL
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHH
SGALSRALRAGPVPPGPLPDDVRALVPVNLRAAPAAGVAGAGNQFGLVFLDLPVEPLAPD
HHHHHHHHHCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCH
ERLEIVRGRTAALKRSPDAWVALGVLGALGFAPPALERLGTAFFSRKASLVVTNVPGPQR
HHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCHHH
RLHLAGRRVDELLFWVPHPAALGLGVSVFSYAGKVTMGVRADAAFPIDPRALAGALDEEL
HHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCEEECEECCCCCCCCHHHHHHHHHHHH
AAFAPRPPARRARPPAAAAAPEQDGRQAAFAGAPAGRGVRVIEAGPATSPAS
HHCCCCCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
TREPMSRVDAAWLRMDRPENTADIVVLLRLAGRLTPPRLRTLLEQRLLPHERFRARVAD
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHC
AGALGGPAWEPDPRFSIARHLSARPLREAHPEALEAAVSEAATQPLPAGRPPWRALLLEG
CCCCCCCCCCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
AEESALVVKLHHCMADGVALVSVLLGLSDEHAGRVEAPAPDVPGGDPLRRGARALLRDPL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAAAALWKMATLPTATGALAPPALSGRRTVAWSRSWPLEDVRAAARAGGGTVNDALLAAL
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHH
SGALSRALRAGPVPPGPLPDDVRALVPVNLRAAPAAGVAGAGNQFGLVFLDLPVEPLAPD
HHHHHHHHHCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCH
ERLEIVRGRTAALKRSPDAWVALGVLGALGFAPPALERLGTAFFSRKASLVVTNVPGPQR
HHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCHHH
RLHLAGRRVDELLFWVPHPAALGLGVSVFSYAGKVTMGVRADAAFPIDPRALAGALDEEL
HHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCEEECEECCCCCCCCHHHHHHHHHHHH
AAFAPRPPARRARPPAAAAAPEQDGRQAAFAGAPAGRGVRVIEAGPATSPAS
HHCCCCCCHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]