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The map label for this gene is prpB [C]

Identifier: 220916793

GI number: 220916793

Start: 1879245

End: 1880084

Strand: Reverse

Name: prpB [C]

Synonym: A2cp1_1688

Alternate gene names: 220916793

Gene position: 1880084-1879245 (Counterclockwise)

Preceding gene: 220916796

Following gene: 220916792

Centisome position: 37.38

GC content: 78.1

Gene sequence:

>840_bases
ATGACCTCGATCGACGAGCGGCGCACCGCCTTCCGCGCGCTCCACCAGCAGGGCTGCTTCGTGCTGCCCAACCCGTGGGA
CGCCGGCACCGCCCGCGCGCTCGAGCGCGCCGGCTTCCGGGCGCTCGCCACCACCAGCGCCGGCTTCGCGTTCTCGCGCG
GCCTGCCCGACGGCGCGGTGGCCGGCGCGGACATGCTCGCGCACGTCCGCGAGCTCGTCGGCGCCACCTCGCTGCCCGTG
AACGCCGACCTGGAGGACGGATACGGCGCCACCGCGGCCGAGGTCGCGGCGAACGTGCGGGCGTGCGTGGACGCCAGGGT
GGCCGGGCTCTCGATCGAGGACGCCACCGGCGATCCGGCGGCGCCGCTGTACCCGGCCGACGAGGCCGCGGCGCGCCTGC
GCGCGGCGCGAGGCGCCATCGACGCGGCGGGCGGCGGGGTGGTGCTCACCTGCCGGACCGAGGGGTTCGTGGTGGGGCGG
CCCGACCTCGCCGAGACCATCCGGCGGCTGGTGGCGTACGCGGAGGCGGGTGCCGACTGCCTCTACGCGCCGGGCCTCAC
CACGCGCGAGCAGGTCGCAGCGGTGGTGAAGGCGGTGGCGCCACGACCGGTGAACGTCCTCGCCGGCGGCCCGGCGTTCA
CCGTCGCCGAGCTGGCCGCGCTCGGCGTCCGCCGCGTCAGCCTCGGCTCCGCGCTGGCGCGCGCGGCGTGGGGCGCGTTC
CTCGAGGCCTCGCGCGAGATCGCCGAGCGCGGCACGTTCGAGCGGCTGGGCGAGGCGGCGGGGTACGCCGCGCTGAACGG
GCTGTTCCGGCCGGACGGTGGGTACGGGCACGCGCCGTGA

Upstream 100 bases:

>100_bases
GCTCCCTCCGAGTCGGTCTCGCAGTAGCGAAGCGCGGGAGGGCCGCGCAAGGCTCCGCCGGGGCCGGGCCGCCGCCCGCG
ACGCCCGCTACGCTGGCGGC

Downstream 100 bases:

>100_bases
CCATCAGCTCGCTTCGCGCCGCACGATCCAGTCCATAATCTCCGCTGATGGCACATCCGTTCTTGCGCGTTCCGCGGTCC
CGGGTTCGCGGGCATGTTGC

Product: PEP phosphonomutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTSIDERRTAFRALHQQGCFVLPNPWDAGTARALERAGFRALATTSAGFAFSRGLPDGAVAGADMLAHVRELVGATSLPV
NADLEDGYGATAAEVAANVRACVDARVAGLSIEDATGDPAAPLYPADEAAARLRAARGAIDAAGGGVVLTCRTEGFVVGR
PDLAETIRRLVAYAEAGADCLYAPGLTTREQVAAVVKAVAPRPVNVLAGGPAFTVAELAALGVRRVSLGSALARAAWGAF
LEASREIAERGTFERLGEAAGYAALNGLFRPDGGYGHAP

Sequences:

>Translated_279_residues
MTSIDERRTAFRALHQQGCFVLPNPWDAGTARALERAGFRALATTSAGFAFSRGLPDGAVAGADMLAHVRELVGATSLPV
NADLEDGYGATAAEVAANVRACVDARVAGLSIEDATGDPAAPLYPADEAAARLRAARGAIDAAGGGVVLTCRTEGFVVGR
PDLAETIRRLVAYAEAGADCLYAPGLTTREQVAAVVKAVAPRPVNVLAGGPAFTVAELAALGVRRVSLGSALARAAWGAF
LEASREIAERGTFERLGEAAGYAALNGLFRPDGGYGHAP
>Mature_278_residues
TSIDERRTAFRALHQQGCFVLPNPWDAGTARALERAGFRALATTSAGFAFSRGLPDGAVAGADMLAHVRELVGATSLPVN
ADLEDGYGATAAEVAANVRACVDARVAGLSIEDATGDPAAPLYPADEAAARLRAARGAIDAAGGGVVLTCRTEGFVVGRP
DLAETIRRLVAYAEAGADCLYAPGLTTREQVAAVVKAVAPRPVNVLAGGPAFTVAELAALGVRRVSLGSALARAAWGAFL
EASREIAERGTFERLGEAAGYAALNGLFRPDGGYGHAP

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=264, Percent_Identity=30.3030303030303, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28542; Mature: 28411

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIDERRTAFRALHQQGCFVLPNPWDAGTARALERAGFRALATTSAGFAFSRGLPDGAV
CCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCHHHHHCCCCCHHHCCCCCHHH
AGADMLAHVRELVGATSLPVNADLEDGYGATAAEVAANVRACVDARVAGLSIEDATGDPA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCC
APLYPADEAAARLRAARGAIDAAGGGVVLTCRTEGFVVGRPDLAETIRRLVAYAEAGADC
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCE
LYAPGLTTREQVAAVVKAVAPRPVNVLAGGPAFTVAELAALGVRRVSLGSALARAAWGAF
EECCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEASREIAERGTFERLGEAAGYAALNGLFRPDGGYGHAP
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSIDERRTAFRALHQQGCFVLPNPWDAGTARALERAGFRALATTSAGFAFSRGLPDGAV
CCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCHHHHHCCCCCHHHCCCCCHHH
AGADMLAHVRELVGATSLPVNADLEDGYGATAAEVAANVRACVDARVAGLSIEDATGDPA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCC
APLYPADEAAARLRAARGAIDAAGGGVVLTCRTEGFVVGRPDLAETIRRLVAYAEAGADC
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCE
LYAPGLTTREQVAAVVKAVAPRPVNVLAGGPAFTVAELAALGVRRVSLGSALARAAWGAF
EECCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEASREIAERGTFERLGEAAGYAALNGLFRPDGGYGHAP
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]