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The map label for this gene is ddlA [C]

Identifier: 220916612

GI number: 220916612

Start: 1685397

End: 1687583

Strand: Direct

Name: ddlA [C]

Synonym: A2cp1_1506

Alternate gene names: 220916612

Gene position: 1685397-1687583 (Clockwise)

Preceding gene: 220916611

Following gene: 220916613

Centisome position: 33.51

GC content: 72.34

Gene sequence:

>2187_bases
ATGCGCATCGCCCTGACCCACAACCTCCGGATCTCGGACTCCGAGGAGGAAGCCGAGTTCGACACCCGCGAGACGGTGGA
CGCGCTCGCCGGCGCCATCGAGCGGCTCGGCCACCGCGTCGAGCGCATCGAGGTCTCCGGCCCGGCGTCGCGCACGGTGA
CGCGCCTCGAGGCCTTCGGGCCGGACCTCATCTTCAACACCGCCGAGGGCCGGCGCGGCCGCTTCCGCGAGGCGTTCTTC
CCGGCGCTGTTCGACGAGCTGGGCATGCCGTACACCGGCTCGGACGCGTACGCGCTCTCGGTGACCCTCGACAAGCAGCT
CACCAAGCTGGTGCTGGCCCAGAAGGGCGTCCGGACGCCGCGCTGGCAGTACGTCGAGGAGCCCTGGCAGCTGCAGGTGA
ACGCGCTCCGCTACCCGGTCATCGTGAAGCCCAACTTCGAGGGCAGCTCGAAGGGCATCACCCAGGACTCGGTGGTGGAC
GATCCCATGCGCCTGCACGAGGTGGTGCAGGACGGGCTGGCGCGCTACCCGGCGGGCCTGCTGGTGGAGGAGTTCGTGGT
CGGGCGCGACCTGACCGTCCCGTTCCTGGAGGCGGCCGCGCCGGAGCGGCACGGCGTGCTCCAGCCGGTCGAGTACGTCA
TCGACCCCGCCGTCACCGCCGGCCGCAAGTACGCGATCTACGACTACGAGCTGAAGACGAAGCTCGACCAGGCGGTGTCG
GTGCGCGCGCCCGCGAAGGTGAAGCGCGCCCAGGCAGAGCGGATCCAGCAGCTCTGCGAGACCGTCTACCGCGAGCTCGG
CATCCGCGACCTGGGGCGCATCGACCTGCGCCTCGGCGACGACGGCGAGCTCTACTTCCTCGAGATCAACGCGCTCCCCT
CGCTGGAGCCCGGGGCCGGCATCTACGCGGCCGCCGCGCTGGAGGGGCTGCACGAGGACGCGGTGCTCGGGGCCGTCATC
CAGAGCGCGGTGCAGCGCTGGAACATCGTGGACAAGAGCGCCCGGCGAGGGCGGCCCCGCCGCACCGAGCGGCTCAAGGT
CGGCTTCAGCTTCAACGTGAAGCGCGTCACCCCCGATCCCGGCGGCGAGCAGGACGAGGAGGCGGAGTACGACTCGCCGA
AGACGCTCCAGGCCATCCGCGAGGCCATCGCGAGCTGGGGCCACGAGGTGGTGGACCTCGAGGCGACCCAGGACCTGCCG
CTGCAGCTCGTGTCCACGCCGGTCGACGTGGTCTTCAACATCGCCGAGGGCTTCAAGGGGCGCAGCCGCGAGTCGCAGGT
GCCGTCCCTGCTCGAGCTGCTCGACATCCCCTACACCGGCTCGGATCCGGCGGCGCTGTCGGTCTCGCTCGACAAGGCGC
TCGCGAAGCGCATGGTGCGCACCCACGGGATCCTCACCCCCGACTACCTGGTGCTGAACACCGGCAACGAGCGGCTGCCG
CGCGAGCTGACCTTCCCGCTCATCGTGAAGCCGGTGGCGGAGGGCACGTCGAAGGGCGTCACGCGGAAGTCGGTGGTCCG
GGACGAGCCCGAGCTGCGCGAGGTGGCCCGGGAGCTCATCGCGAAGTACCGCCAGCCGGCGCTGGCGGAGGGCTACGTCT
CCGGCCGCGAGTTCACCGTCGGGCTGCTGGGCGAGCGGCGGCCGCGGGTGCTCCCGCCCATGGAGATCGTGTTCCTCGAC
GCGAAGGACCCCACGCCCATCTACTCGTTCGAGATGAAGCAGGACTGGAACGAGCGGATCCGCTACGAGGTGCCGGCGAA
GCTCACGCCGCGCGAGCTGGAGCGCCTGGAGAAGGCGGCGCGCGAGTGCTTCACCGCGCTCGGCTGCCGCGACGTGGCGC
GGCTCGACTTCCGCATGGACGCCGAGGGGCGGATCCACTTCATCGAGTGCAACCCGCTCCCCGGCCTGGCCCCCGGCTGG
TCGGACCTCGTGCTCATCGCGCAGGCCGCCGGCATGGACTACCGCGCGCTCATCGGCGAGATCCTCTCGTTCGCGATCCG
GCGGTACCAGGAGCGCGAGCGCGAGCGCGAGCGGGCCCGCCGCGCGCAGGCCGCGGCCGAGCGCGAGGCGCTGGCGACGG
CGGCGCAGGGGAACGGCGCGCCCGGCGCGGAGGCGCCCGCCGGCGGGAACGGCGGCTCGGGCGGCGAGGCTGGCGAGCGG
CGGCCGCAGGCGCCGCACCGGCACTGA

Upstream 100 bases:

>100_bases
GCGAGCGCCACGCGCCAGCGCGCGGCCGCATGAAACCCCCACCAGCCAGCGTCGTCCCGCCTCGAGCCCCGCGTCCCCAC
GGGCCGGGAGAGGTCTCGCC

Downstream 100 bases:

>100_bases
TCTCGCGGCGCCGCGCCCCGGCGGGAGCCCCGCGGCAGGACCGCCCATCCGCGCCCCGGCGGGATTTCCGCTTCGCCCGG
GGCCGCGGGTGGCGTAAGTC

Product: D-alanine--D-alanine ligase

Products: ADP; phosphate; D-alanyl-D-alanine

Alternate protein names: NA

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MRIALTHNLRISDSEEEAEFDTRETVDALAGAIERLGHRVERIEVSGPASRTVTRLEAFGPDLIFNTAEGRRGRFREAFF
PALFDELGMPYTGSDAYALSVTLDKQLTKLVLAQKGVRTPRWQYVEEPWQLQVNALRYPVIVKPNFEGSSKGITQDSVVD
DPMRLHEVVQDGLARYPAGLLVEEFVVGRDLTVPFLEAAAPERHGVLQPVEYVIDPAVTAGRKYAIYDYELKTKLDQAVS
VRAPAKVKRAQAERIQQLCETVYRELGIRDLGRIDLRLGDDGELYFLEINALPSLEPGAGIYAAAALEGLHEDAVLGAVI
QSAVQRWNIVDKSARRGRPRRTERLKVGFSFNVKRVTPDPGGEQDEEAEYDSPKTLQAIREAIASWGHEVVDLEATQDLP
LQLVSTPVDVVFNIAEGFKGRSRESQVPSLLELLDIPYTGSDPAALSVSLDKALAKRMVRTHGILTPDYLVLNTGNERLP
RELTFPLIVKPVAEGTSKGVTRKSVVRDEPELREVARELIAKYRQPALAEGYVSGREFTVGLLGERRPRVLPPMEIVFLD
AKDPTPIYSFEMKQDWNERIRYEVPAKLTPRELERLEKAARECFTALGCRDVARLDFRMDAEGRIHFIECNPLPGLAPGW
SDLVLIAQAAGMDYRALIGEILSFAIRRYQERERERERARRAQAAAEREALATAAQGNGAPGAEAPAGGNGGSGGEAGER
RPQAPHRH

Sequences:

>Translated_728_residues
MRIALTHNLRISDSEEEAEFDTRETVDALAGAIERLGHRVERIEVSGPASRTVTRLEAFGPDLIFNTAEGRRGRFREAFF
PALFDELGMPYTGSDAYALSVTLDKQLTKLVLAQKGVRTPRWQYVEEPWQLQVNALRYPVIVKPNFEGSSKGITQDSVVD
DPMRLHEVVQDGLARYPAGLLVEEFVVGRDLTVPFLEAAAPERHGVLQPVEYVIDPAVTAGRKYAIYDYELKTKLDQAVS
VRAPAKVKRAQAERIQQLCETVYRELGIRDLGRIDLRLGDDGELYFLEINALPSLEPGAGIYAAAALEGLHEDAVLGAVI
QSAVQRWNIVDKSARRGRPRRTERLKVGFSFNVKRVTPDPGGEQDEEAEYDSPKTLQAIREAIASWGHEVVDLEATQDLP
LQLVSTPVDVVFNIAEGFKGRSRESQVPSLLELLDIPYTGSDPAALSVSLDKALAKRMVRTHGILTPDYLVLNTGNERLP
RELTFPLIVKPVAEGTSKGVTRKSVVRDEPELREVARELIAKYRQPALAEGYVSGREFTVGLLGERRPRVLPPMEIVFLD
AKDPTPIYSFEMKQDWNERIRYEVPAKLTPRELERLEKAARECFTALGCRDVARLDFRMDAEGRIHFIECNPLPGLAPGW
SDLVLIAQAAGMDYRALIGEILSFAIRRYQERERERERARRAQAAAEREALATAAQGNGAPGAEAPAGGNGGSGGEAGER
RPQAPHRH
>Mature_728_residues
MRIALTHNLRISDSEEEAEFDTRETVDALAGAIERLGHRVERIEVSGPASRTVTRLEAFGPDLIFNTAEGRRGRFREAFF
PALFDELGMPYTGSDAYALSVTLDKQLTKLVLAQKGVRTPRWQYVEEPWQLQVNALRYPVIVKPNFEGSSKGITQDSVVD
DPMRLHEVVQDGLARYPAGLLVEEFVVGRDLTVPFLEAAAPERHGVLQPVEYVIDPAVTAGRKYAIYDYELKTKLDQAVS
VRAPAKVKRAQAERIQQLCETVYRELGIRDLGRIDLRLGDDGELYFLEINALPSLEPGAGIYAAAALEGLHEDAVLGAVI
QSAVQRWNIVDKSARRGRPRRTERLKVGFSFNVKRVTPDPGGEQDEEAEYDSPKTLQAIREAIASWGHEVVDLEATQDLP
LQLVSTPVDVVFNIAEGFKGRSRESQVPSLLELLDIPYTGSDPAALSVSLDKALAKRMVRTHGILTPDYLVLNTGNERLP
RELTFPLIVKPVAEGTSKGVTRKSVVRDEPELREVARELIAKYRQPALAEGYVSGREFTVGLLGERRPRVLPPMEIVFLD
AKDPTPIYSFEMKQDWNERIRYEVPAKLTPRELERLEKAARECFTALGCRDVARLDFRMDAEGRIHFIECNPLPGLAPGW
SDLVLIAQAAGMDYRALIGEILSFAIRRYQERERERERARRAQAAAEREALATAAQGNGAPGAEAPAGGNGGSGGEAGER
RPQAPHRH

Specific function: Could be involved in the biosynthesis of a cell wall component [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=288, Percent_Identity=27.0833333333333, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1786280, Length=273, Percent_Identity=29.6703296703297, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: 6.3.2.4

Molecular weight: Translated: 80929; Mature: 80929

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALTHNLRISDSEEEAEFDTRETVDALAGAIERLGHRVERIEVSGPASRTVTRLEAFG
CEEEEEECEEECCCHHHHHCCHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHCC
PDLIFNTAEGRRGRFREAFFPALFDELGMPYTGSDAYALSVTLDKQLTKLVLAQKGVRTP
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCC
RWQYVEEPWQLQVNALRYPVIVKPNFEGSSKGITQDSVVDDPMRLHEVVQDGLARYPAGL
CHHHCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
LVEEFVVGRDLTVPFLEAAAPERHGVLQPVEYVIDPAVTAGRKYAIYDYELKTKLDQAVS
HHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHCCHHHCCCEEEEEEEHHHHHHHHHHC
VRAPAKVKRAQAERIQQLCETVYRELGIRDLGRIDLRLGDDGELYFLEINALPSLEPGAG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCC
IYAAAALEGLHEDAVLGAVIQSAVQRWNIVDKSARRGRPRRTERLKVGFSFNVKRVTPDP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEEEEEEEECCCC
GGEQDEEAEYDSPKTLQAIREAIASWGHEVVDLEATQDLPLQLVSTPVDVVFNIAEGFKG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHHHHHHCCCC
RSRESQVPSLLELLDIPYTGSDPAALSVSLDKALAKRMVRTHGILTPDYLVLNTGNERLP
CCCHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
RELTFPLIVKPVAEGTSKGVTRKSVVRDEPELREVARELIAKYRQPALAEGYVSGREFTV
CCCCCHHEEEEHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEE
GLLGERRPRVLPPMEIVFLDAKDPTPIYSFEMKQDWNERIRYEVPAKLTPRELERLEKAA
EECCCCCCCCCCCEEEEEEECCCCCCEEEEEHHHCHHCCCEEECCCCCCHHHHHHHHHHH
RECFTALGCRDVARLDFRMDAEGRIHFIECNPLPGLAPGWSDLVLIAQAAGMDYRALIGE
HHHHHHHCHHHHHHCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
ILSFAIRRYQERERERERARRAQAAAEREALATAAQGNGAPGAEAPAGGNGGSGGEAGER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
RPQAPHRH
CCCCCCCC
>Mature Secondary Structure
MRIALTHNLRISDSEEEAEFDTRETVDALAGAIERLGHRVERIEVSGPASRTVTRLEAFG
CEEEEEECEEECCCHHHHHCCHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHCC
PDLIFNTAEGRRGRFREAFFPALFDELGMPYTGSDAYALSVTLDKQLTKLVLAQKGVRTP
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCC
RWQYVEEPWQLQVNALRYPVIVKPNFEGSSKGITQDSVVDDPMRLHEVVQDGLARYPAGL
CHHHCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
LVEEFVVGRDLTVPFLEAAAPERHGVLQPVEYVIDPAVTAGRKYAIYDYELKTKLDQAVS
HHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHCCHHHCCCEEEEEEEHHHHHHHHHHC
VRAPAKVKRAQAERIQQLCETVYRELGIRDLGRIDLRLGDDGELYFLEINALPSLEPGAG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCC
IYAAAALEGLHEDAVLGAVIQSAVQRWNIVDKSARRGRPRRTERLKVGFSFNVKRVTPDP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEEEEEEEECCCC
GGEQDEEAEYDSPKTLQAIREAIASWGHEVVDLEATQDLPLQLVSTPVDVVFNIAEGFKG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHHHHHHCCCC
RSRESQVPSLLELLDIPYTGSDPAALSVSLDKALAKRMVRTHGILTPDYLVLNTGNERLP
CCCHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
RELTFPLIVKPVAEGTSKGVTRKSVVRDEPELREVARELIAKYRQPALAEGYVSGREFTV
CCCCCHHEEEEHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEE
GLLGERRPRVLPPMEIVFLDAKDPTPIYSFEMKQDWNERIRYEVPAKLTPRELERLEKAA
EECCCCCCCCCCCEEEEEEECCCCCCEEEEEHHHCHHCCCEEECCCCCCHHHHHHHHHHH
RECFTALGCRDVARLDFRMDAEGRIHFIECNPLPGLAPGWSDLVLIAQAAGMDYRALIGE
HHHHHHHCHHHHHHCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
ILSFAIRRYQERERERERARRAQAAAEREALATAAQGNGAPGAEAPAGGNGGSGGEAGER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
RPQAPHRH
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-alanine

Specific reaction: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: (3-Amino-2-oxoalkyl) phosphonic acids; alpha-Aminophosphonamidic acid; D-(1-Aminoethyl) phosphonic acid in presence of ATP; D-Cycloserine; Tabtoxinine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]