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The map label for this gene is mrdA [H]

Identifier: 220916560

GI number: 220916560

Start: 1627447

End: 1629501

Strand: Direct

Name: mrdA [H]

Synonym: A2cp1_1454

Alternate gene names: 220916560

Gene position: 1627447-1629501 (Clockwise)

Preceding gene: 220916559

Following gene: 220916561

Centisome position: 32.36

GC content: 73.09

Gene sequence:

>2055_bases
ATGTCGCTCATCCCGCCCAGCGCGCCCGGTGCGGCGCAGAAGGAGTTCAGGCCGCGGCTGGCCTGGGCCACGGGCCTGGT
GGTGCTCGTGTTCCTGACGCTGCTCGGGCGTCTGTACCAGCTCCAGATCCTCCGCGGCGACGAGTACAAGGAGCGCGCCG
ACGAGAACTTCGTGAAGGAGCTGCGCATCCCCGCCGACCGCGGCCTGATGCTCGACCGCAACCGGCGCGTGCTGGTGGAC
TCGCGGCCCTCGTACGACGTGACGCTCACGCCGTACTTCTGCGGGAAGCAGTGCGACGAGATCCTGGCGCGCGTGGCGGC
GCTGCTGCAGATGTCGCCCGAGGAGGTGGCCCGCGCGCAGGGGCAGCTCCGCTCGGCGCGCAAGCTCGAGCGCTTCCGCC
CGTTCACCGTGAAGGTGGACATCACGCGCGAGGAGCTGGACCGGTACCTGGCGCGCCAGATGGACCTGCCCGGCGTGGAC
GTCCAGCCCATCCCGCACCGCAACTACCGCTTCGGCGGGCTGGGCGGGCACCTCATCGGCTACATGAACGAGGTCGGGGG
CGAGGAGCTGAAGCGCCTCAACGAGGAGCTGCAGCGCACCGCCTCGCCGCAGGGCCCCTACTACATGGGCGACTACGTCG
GCCGCCGCGGCCTGGAGCGCCGCTTCGAGCGCGACCTGCGCGGCGTGGACGGCAAGGAGCGCGTGCCGGTGGACGCGAAG
GGGCGCCGCAAGGAGGACGCGGACGACCTCATCCCGCCCGACCAGCGCGTGGTCCCGAGCGTGCCCGGGCGCAACCTGGT
GCTGTCCATCGACTGGCGCCTGCAGGAGTTCGCCGAGCAGACGTTCCCCGCCACCGCCGGCGTGGTGCTGGCGATGGACG
CGAAGACCGGCTTCCTGCTCGCGCTGGTGGACCGCCCCGCGCCGGATCCGAACAAGATGTCCGGACGCATCACCGCGGCC
GAGCTGGCGGCGATCCACTCGGACCCGCTGCAGCCCGAGCTGTTCCGCGCCATCCAGCAGCACTACCACCCCGGCTCCAC
CTTCAAGGCGCTCACCAGCATCGCGGCGCTGGAGGAGGGGGTCTACAAGCCGAACACCTCGGTGTACTGCCCGGGGCACT
TCAGCATGGGCCGCGCGCGCTGGCGCTGCGACAAGGACAGCGGCCACGGCTACGTGGACTTCGAGCACGCGCTGGGCGCG
AGCTGCGACGTGTTCTTCTACGCGGCCGGCGCGCAGCTCGGCGCCGACGCCATCGCGAAGTGGGGCCACCTCTCCGGCCT
GGGCGTGCCCACCGGGTTCGACATCGCCGGCGAGGTGCCGGGCGTGGTGCCCGACGCGGCCTGGCACGACAAGCACCAGG
CCGGCGGCTACCAGCGCGGCATGGCGGTGAACCTGTCCATCGGCCAGGGCGACGTGAACGTCACGCCCATGCAGCAGCTC
GTGTTCTACGGCGCGCTCGCCACCGGCAAGATCTGGAAGCCGCAGGTGGTGCTGCGCGTGGAGGACCCGGACGGCCAGGT
GCTGCAGGCGTTCACGCCGGAGACGCGCGGCGAGCTGCCGGTCAAGCCGTCCACCCGCGACACCGTGCTGAAGGGGCTGC
TCGCGGCGGTGAACCAGCCGTTCGGCACCGCCTACTCGCAGCGCTCCGCCGACTACGTCATGGCGGGCAAGACCGGGACG
GCGCAGGTGGTGAAGCTGGGCGCGCGGCGCCTCAAGGCGTCGCAGGTGTCGTACTTCGAGCGCGACCACGCGTGGTTCGC
GGCGTTCGCGCCGGCGGAGGATCCGGAGATCGTGGTGGTGGTCCTGAACGAGCACTCGGGCTTCGGCTCGTCGAACGCGG
CGCCGACCGCGTCGGCGCTGGTGAAGCGGTACTTCCAGCTGAAGGCGGAGGACGCGGCCGAGCGGGCCGGCCCGGCGCAG
GGCCCGGCGCCCGAGCGCGCCCCGGCGCCTCCCGCCGCGCCGCCCGCGGCGCCGCCGCCCGGCGCGGTCGAGCCGGCCAG
GCTGGGCACGGGCGGCGCGTCCGCCGCGGTGCCGGGGGTGGACCGTGGCGCCTGA

Upstream 100 bases:

>100_bases
GAGGCGCTCCTCACCGCCGCCGTGGCCCCGCTGATCGGGGCGGGCATGCGCCGCATCGACGGCCTGTTCCACCGCGAGGA
GCCGGGGCTCCTGCGCTGAC

Downstream 100 bases:

>100_bases
CGTCTCGCTCGCGGCCACGCCGCACCGGCGCGTGTTCGCGCACTTCCCCTGGCACCTCGCGCTGATCGTCCTGTCGATCT
CGGCGATCGGGATCTGGAAC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MSLIPPSAPGAAQKEFRPRLAWATGLVVLVFLTLLGRLYQLQILRGDEYKERADENFVKELRIPADRGLMLDRNRRVLVD
SRPSYDVTLTPYFCGKQCDEILARVAALLQMSPEEVARAQGQLRSARKLERFRPFTVKVDITREELDRYLARQMDLPGVD
VQPIPHRNYRFGGLGGHLIGYMNEVGGEELKRLNEELQRTASPQGPYYMGDYVGRRGLERRFERDLRGVDGKERVPVDAK
GRRKEDADDLIPPDQRVVPSVPGRNLVLSIDWRLQEFAEQTFPATAGVVLAMDAKTGFLLALVDRPAPDPNKMSGRITAA
ELAAIHSDPLQPELFRAIQQHYHPGSTFKALTSIAALEEGVYKPNTSVYCPGHFSMGRARWRCDKDSGHGYVDFEHALGA
SCDVFFYAAGAQLGADAIAKWGHLSGLGVPTGFDIAGEVPGVVPDAAWHDKHQAGGYQRGMAVNLSIGQGDVNVTPMQQL
VFYGALATGKIWKPQVVLRVEDPDGQVLQAFTPETRGELPVKPSTRDTVLKGLLAAVNQPFGTAYSQRSADYVMAGKTGT
AQVVKLGARRLKASQVSYFERDHAWFAAFAPAEDPEIVVVVLNEHSGFGSSNAAPTASALVKRYFQLKAEDAAERAGPAQ
GPAPERAPAPPAAPPAAPPPGAVEPARLGTGGASAAVPGVDRGA

Sequences:

>Translated_684_residues
MSLIPPSAPGAAQKEFRPRLAWATGLVVLVFLTLLGRLYQLQILRGDEYKERADENFVKELRIPADRGLMLDRNRRVLVD
SRPSYDVTLTPYFCGKQCDEILARVAALLQMSPEEVARAQGQLRSARKLERFRPFTVKVDITREELDRYLARQMDLPGVD
VQPIPHRNYRFGGLGGHLIGYMNEVGGEELKRLNEELQRTASPQGPYYMGDYVGRRGLERRFERDLRGVDGKERVPVDAK
GRRKEDADDLIPPDQRVVPSVPGRNLVLSIDWRLQEFAEQTFPATAGVVLAMDAKTGFLLALVDRPAPDPNKMSGRITAA
ELAAIHSDPLQPELFRAIQQHYHPGSTFKALTSIAALEEGVYKPNTSVYCPGHFSMGRARWRCDKDSGHGYVDFEHALGA
SCDVFFYAAGAQLGADAIAKWGHLSGLGVPTGFDIAGEVPGVVPDAAWHDKHQAGGYQRGMAVNLSIGQGDVNVTPMQQL
VFYGALATGKIWKPQVVLRVEDPDGQVLQAFTPETRGELPVKPSTRDTVLKGLLAAVNQPFGTAYSQRSADYVMAGKTGT
AQVVKLGARRLKASQVSYFERDHAWFAAFAPAEDPEIVVVVLNEHSGFGSSNAAPTASALVKRYFQLKAEDAAERAGPAQ
GPAPERAPAPPAAPPAAPPPGAVEPARLGTGGASAAVPGVDRGA
>Mature_683_residues
SLIPPSAPGAAQKEFRPRLAWATGLVVLVFLTLLGRLYQLQILRGDEYKERADENFVKELRIPADRGLMLDRNRRVLVDS
RPSYDVTLTPYFCGKQCDEILARVAALLQMSPEEVARAQGQLRSARKLERFRPFTVKVDITREELDRYLARQMDLPGVDV
QPIPHRNYRFGGLGGHLIGYMNEVGGEELKRLNEELQRTASPQGPYYMGDYVGRRGLERRFERDLRGVDGKERVPVDAKG
RRKEDADDLIPPDQRVVPSVPGRNLVLSIDWRLQEFAEQTFPATAGVVLAMDAKTGFLLALVDRPAPDPNKMSGRITAAE
LAAIHSDPLQPELFRAIQQHYHPGSTFKALTSIAALEEGVYKPNTSVYCPGHFSMGRARWRCDKDSGHGYVDFEHALGAS
CDVFFYAAGAQLGADAIAKWGHLSGLGVPTGFDIAGEVPGVVPDAAWHDKHQAGGYQRGMAVNLSIGQGDVNVTPMQQLV
FYGALATGKIWKPQVVLRVEDPDGQVLQAFTPETRGELPVKPSTRDTVLKGLLAAVNQPFGTAYSQRSADYVMAGKTGTA
QVVKLGARRLKASQVSYFERDHAWFAAFAPAEDPEIVVVVLNEHSGFGSSNAAPTASALVKRYFQLKAEDAAERAGPAQG
PAPERAPAPPAAPPAAPPPGAVEPARLGTGGASAAVPGVDRGA

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=622, Percent_Identity=29.0996784565916, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1786272, Length=642, Percent_Identity=24.4548286604361, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 74390; Mature: 74259

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIPPSAPGAAQKEFRPRLAWATGLVVLVFLTLLGRLYQLQILRGDEYKERADENFVKE
CCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHH
LRIPADRGLMLDRNRRVLVDSRPSYDVTLTPYFCGKQCDEILARVAALLQMSPEEVARAQ
HCCCCCCCEEEECCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GQLRSARKLERFRPFTVKVDITREELDRYLARQMDLPGVDVQPIPHRNYRFGGLGGHLIG
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCHHHHH
YMNEVGGEELKRLNEELQRTASPQGPYYMGDYVGRRGLERRFERDLRGVDGKERVPVDAK
HHHHCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GRRKEDADDLIPPDQRVVPSVPGRNLVLSIDWRLQEFAEQTFPATAGVVLAMDAKTGFLL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCEEE
ALVDRPAPDPNKMSGRITAAELAAIHSDPLQPELFRAIQQHYHPGSTFKALTSIAALEEG
EEECCCCCCCCCCCCCEEHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
VYKPNTSVYCPGHFSMGRARWRCDKDSGHGYVDFEHALGASCDVFFYAAGAQLGADAIAK
CCCCCCCEEECCCCCCCCCEEEECCCCCCCEEEEHHHCCCCCEEEEEECCCHHCHHHHHH
WGHLSGLGVPTGFDIAGEVPGVVPDAAWHDKHQAGGYQRGMAVNLSIGQGDVNVTPMQQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEEECCCCCCCCHHHHH
VFYGALATGKIWKPQVVLRVEDPDGQVLQAFTPETRGELPVKPSTRDTVLKGLLAAVNQP
HHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
FGTAYSQRSADYVMAGKTGTAQVVKLGARRLKASQVSYFERDHAWFAAFAPAEDPEIVVV
CCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEE
VLNEHSGFGSSNAAPTASALVKRYFQLKAEDAAERAGPAQGPAPERAPAPPAAPPAAPPP
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GAVEPARLGTGGASAAVPGVDRGA
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLIPPSAPGAAQKEFRPRLAWATGLVVLVFLTLLGRLYQLQILRGDEYKERADENFVKE
CCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHH
LRIPADRGLMLDRNRRVLVDSRPSYDVTLTPYFCGKQCDEILARVAALLQMSPEEVARAQ
HCCCCCCCEEEECCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GQLRSARKLERFRPFTVKVDITREELDRYLARQMDLPGVDVQPIPHRNYRFGGLGGHLIG
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCHHHHH
YMNEVGGEELKRLNEELQRTASPQGPYYMGDYVGRRGLERRFERDLRGVDGKERVPVDAK
HHHHCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GRRKEDADDLIPPDQRVVPSVPGRNLVLSIDWRLQEFAEQTFPATAGVVLAMDAKTGFLL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCEEE
ALVDRPAPDPNKMSGRITAAELAAIHSDPLQPELFRAIQQHYHPGSTFKALTSIAALEEG
EEECCCCCCCCCCCCCEEHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
VYKPNTSVYCPGHFSMGRARWRCDKDSGHGYVDFEHALGASCDVFFYAAGAQLGADAIAK
CCCCCCCEEECCCCCCCCCEEEECCCCCCCEEEEHHHCCCCCEEEEEECCCHHCHHHHHH
WGHLSGLGVPTGFDIAGEVPGVVPDAAWHDKHQAGGYQRGMAVNLSIGQGDVNVTPMQQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEEECCCCCCCCHHHHH
VFYGALATGKIWKPQVVLRVEDPDGQVLQAFTPETRGELPVKPSTRDTVLKGLLAAVNQP
HHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
FGTAYSQRSADYVMAGKTGTAQVVKLGARRLKASQVSYFERDHAWFAAFAPAEDPEIVVV
CCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEE
VLNEHSGFGSSNAAPTASALVKRYFQLKAEDAAERAGPAQGPAPERAPAPPAAPPAAPPP
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
GAVEPARLGTGGASAAVPGVDRGA
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]