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The map label for this gene is tgt [H]

Identifier: 220916514

GI number: 220916514

Start: 1573600

End: 1574763

Strand: Direct

Name: tgt [H]

Synonym: A2cp1_1408

Alternate gene names: 220916514

Gene position: 1573600-1574763 (Clockwise)

Preceding gene: 220916513

Following gene: 220916515

Centisome position: 31.29

GC content: 75.26

Gene sequence:

>1164_bases
GTGATCCGCTTCCAGCTCCTCGCCGAGGACGCCGCCGCCCGCCGCGGCCGGCTCGAGACCGCGCACGGCACCATCGAGAC
GCCGGTGTTCATGCCGGTGGGCACCGCCGCCACGGTGAAGACGCTCGAGCCGCGCGACCTCCAGGCGCTGGGCGCCGGCA
TCGTGCTCGCGAACACGTACCACCTGTACCTGCGCCCCGGGCACGAGCGGATCCAGCGCCTCGGCCGGCTGCACCGCTTC
ATGTCCTGGCCGGGCGCGGTGCTCACCGACTCGGGCGGCTTCCAGGTCTTCAGCCTGGGCGAGAAGGGCGCCGGCCGCGA
GCGCGGCGGGCCCGGCCTGGTCGAGATCGCGGAGGAGGGCGTCACCTTCCGCAGCCACCTCGACGGCTCGCGCCACTTCC
TCTCGCCGGAGCGGGCGATCGAGGTCCAGGAGGCGCTCGGCGCCGACGTGATCATGGCGTTCGACGAGTGCCCGCCGGCG
CTCGCCGACCGCGCCTACCACGAGCTGTCGCTGGCGCGGACGCAGCGCTGGCTGGTGCGGTGCCGCGAGGCCTGGCGCGA
GATCGAGGCGCGCGAGGGGCCGCGCTGCGCGCTGTTCGGCATCGCGCAGGGCGGGCTGTTCGGAGACCTGCGGGCGCGGG
CCATCGAGGAGGCGGCCGCGCTGGACCTGCCCGGCTACGCGCTGGGCGGCTACGCGGTGGGCGAGACGCCGGCCGAGATG
TGGGACGGCGTGGCCCGGGACGCGCCGCGGCTGCCGCGCGAGAAGCCGCGCTACCTCATGGGCGTGGGCACGCCGGAGGA
CCTGCTGGCGGGGATCGCCGCCGGCGTGGACCTGTTCGACTGCGTGCTCCCCACCCGCACCGCGCGCAACGGCCTGCTGT
TCACGAGCCGCGGCAAGCTCGTGATCCGCAACGCGCGCTACGCGGACGACGAGCGGCCGGCCGATCCGGAGTGCGGGTGC
TACGCCTGCCGGACGTTCACCCGCGCCTACCTCCGCCACCTGTTCAAGGCCGGCGAGATCCTGGCGCTCCGCGCGAACAC
CCTGCACAACCTGCACTTCTACCTGTCGCTCATGGCCGACGCCCGGCGCGCCATCGAGGAGGGCCGGTTCGCGGCGTTCC
GCGCCGAGCGGCTGGCCGCCTGGCGCGCGGACGGCGACGGCTGA

Upstream 100 bases:

>100_bases
GGGCGCGCGGGCCGGGCCTGGCGCGGCGTGGTGGCCGGGGTGGCGCGCGTGGGGCGGCGCCCGGCTTGACAGGCCCGGCG
GCGCGGGCGACACCGGCCCG

Downstream 100 bases:

>100_bases
CGGGGCCCGCCGGCCGGGTGCGCGCGGCCGCGCACGCCCGGGGGCCCCGGCGGGGAACAAAGGGGTTGCGGCCCTCGTCG
AAATCACGCTAGAACACAGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MIRFQLLAEDAAARRGRLETAHGTIETPVFMPVGTAATVKTLEPRDLQALGAGIVLANTYHLYLRPGHERIQRLGRLHRF
MSWPGAVLTDSGGFQVFSLGEKGAGRERGGPGLVEIAEEGVTFRSHLDGSRHFLSPERAIEVQEALGADVIMAFDECPPA
LADRAYHELSLARTQRWLVRCREAWREIEAREGPRCALFGIAQGGLFGDLRARAIEEAAALDLPGYALGGYAVGETPAEM
WDGVARDAPRLPREKPRYLMGVGTPEDLLAGIAAGVDLFDCVLPTRTARNGLLFTSRGKLVIRNARYADDERPADPECGC
YACRTFTRAYLRHLFKAGEILALRANTLHNLHFYLSLMADARRAIEEGRFAAFRAERLAAWRADGDG

Sequences:

>Translated_387_residues
MIRFQLLAEDAAARRGRLETAHGTIETPVFMPVGTAATVKTLEPRDLQALGAGIVLANTYHLYLRPGHERIQRLGRLHRF
MSWPGAVLTDSGGFQVFSLGEKGAGRERGGPGLVEIAEEGVTFRSHLDGSRHFLSPERAIEVQEALGADVIMAFDECPPA
LADRAYHELSLARTQRWLVRCREAWREIEAREGPRCALFGIAQGGLFGDLRARAIEEAAALDLPGYALGGYAVGETPAEM
WDGVARDAPRLPREKPRYLMGVGTPEDLLAGIAAGVDLFDCVLPTRTARNGLLFTSRGKLVIRNARYADDERPADPECGC
YACRTFTRAYLRHLFKAGEILALRANTLHNLHFYLSLMADARRAIEEGRFAAFRAERLAAWRADGDG
>Mature_387_residues
MIRFQLLAEDAAARRGRLETAHGTIETPVFMPVGTAATVKTLEPRDLQALGAGIVLANTYHLYLRPGHERIQRLGRLHRF
MSWPGAVLTDSGGFQVFSLGEKGAGRERGGPGLVEIAEEGVTFRSHLDGSRHFLSPERAIEVQEALGADVIMAFDECPPA
LADRAYHELSLARTQRWLVRCREAWREIEAREGPRCALFGIAQGGLFGDLRARAIEEAAALDLPGYALGGYAVGETPAEM
WDGVARDAPRLPREKPRYLMGVGTPEDLLAGIAAGVDLFDCVLPTRTARNGLLFTSRGKLVIRNARYADDERPADPECGC
YACRTFTRAYLRHLFKAGEILALRANTLHNLHFYLSLMADARRAIEEGRFAAFRAERLAAWRADGDG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=372, Percent_Identity=44.8924731182796, Blast_Score=314, Evalue=7e-86,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=49.1935483870968, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17544680, Length=381, Percent_Identity=39.8950131233596, Blast_Score=270, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI25146502, Length=184, Percent_Identity=27.1739130434783, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920510, Length=380, Percent_Identity=41.0526315789474, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42609; Mature: 42609

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFQLLAEDAAARRGRLETAHGTIETPVFMPVGTAATVKTLEPRDLQALGAGIVLANTY
CEEEEEEHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEECCCCCHHHHHCCCEEEEEEE
HLYLRPGHERIQRLGRLHRFMSWPGAVLTDSGGFQVFSLGEKGAGRERGGPGLVEIAEEG
EEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCC
VTFRSHLDGSRHFLSPERAIEVQEALGADVIMAFDECPPALADRAYHELSLARTQRWLVR
CEEHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
CREAWREIEAREGPRCALFGIAQGGLFGDLRARAIEEAAALDLPGYALGGYAVGETPAEM
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECCEECCCCHHHH
WDGVARDAPRLPREKPRYLMGVGTPEDLLAGIAAGVDLFDCVLPTRTARNGLLFTSRGKL
HCCHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCHHHHHHHCCCCCCCCCEEEECCCCE
VIRNARYADDERPADPECGCYACRTFTRAYLRHLFKAGEILALRANTLHNLHFYLSLMAD
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
ARRAIEEGRFAAFRAERLAAWRADGDG
HHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIRFQLLAEDAAARRGRLETAHGTIETPVFMPVGTAATVKTLEPRDLQALGAGIVLANTY
CEEEEEEHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEECCCCCHHHHHCCCEEEEEEE
HLYLRPGHERIQRLGRLHRFMSWPGAVLTDSGGFQVFSLGEKGAGRERGGPGLVEIAEEG
EEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCC
VTFRSHLDGSRHFLSPERAIEVQEALGADVIMAFDECPPALADRAYHELSLARTQRWLVR
CEEHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
CREAWREIEAREGPRCALFGIAQGGLFGDLRARAIEEAAALDLPGYALGGYAVGETPAEM
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEECCEECCCCHHHH
WDGVARDAPRLPREKPRYLMGVGTPEDLLAGIAAGVDLFDCVLPTRTARNGLLFTSRGKL
HCCHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCHHHHHHHCCCCCCCCCEEEECCCCE
VIRNARYADDERPADPECGCYACRTFTRAYLRHLFKAGEILALRANTLHNLHFYLSLMAD
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
ARRAIEEGRFAAFRAERLAAWRADGDG
HHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA