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The map label for this gene is queA

Identifier: 220916512

GI number: 220916512

Start: 1571837

End: 1572874

Strand: Direct

Name: queA

Synonym: A2cp1_1406

Alternate gene names: 220916512

Gene position: 1571837-1572874 (Clockwise)

Preceding gene: 220916511

Following gene: 220916513

Centisome position: 31.25

GC content: 77.26

Gene sequence:

>1038_bases
GTGCGCCTCTCCGACTTCGACTTCGCCCTGCCCGAGGGGCTGGTGGCCCAGGCGCCGGTCACGCCGCGCGACGCCTCGCG
CCTCATGGTGCTCGCGCCGGAGGAGGGCTCGCCCGCGCACCGCGGCTTCGCCGACCTGCCGGAGCTGCTCGCGCCGGGCG
ACCTGCTCGTCTTCAACGACACGCGCGTCATCCCGGCGCGGCTGCTCGGCCACAAGGCGTCGGGCGGCAAGGTGGAGCTG
CTCCTGTGCGAGCCGCTGGAGGGCGGGCTGGGCCGGCGCTGGCGGGCCATGGGGCAGGCCTCGAAGCCCATCCGCGAGGG
CGCGGTGCTCACCTTCGACGGCCTCGAGGCGCGCGTCGACGACGTGGAGGGCGAGGGCTTCTACCGGGTCACGCTGGACC
GCCAGGGCCCCGAGCTGGAGGCGGCGCTGGGCCGCGCCGGGCGCATCCCGCTGCCGCCGTACATCCGCCGCGCGCCGGAC
GCCGAGGACGCGGCGCGCTACCAGACCATCTGGGCGCGGGCGCCCGGCTCCGCCGCCGCGCCGACCGCCGGCCTGCACTT
CACCGAGCCGCTGCTGGCGCGCCTCGCGGCGCGCGGGATCCGGCGCACCGCGGTCACGCTGCACGTCGGCCCGGGCACGT
TCCTGCCCATCCGCGGCGACGACCTCGACCTGCACCGCATGCACGGCGAGCGCTACGAGGTGTCGCCCGCCGCCGCGGCG
GAGCTCGCCGCGACCCGCGCGCGCGGCGGACGCATCGTGGCGGTGGGCACCACCTCGGTGCGCACGCTGGAGAGCGCCTG
GCGCGACGGCGCCGTCGCCGCCGGCCCGGGCCGCACCGAGCTGTTCATCCGCCCCGGCCACCCGTTCCACGCGGTGGACG
CGATGGTCACGAACTTCCACCTCCCCCGTTCCACGCTGCTGGTGCTGGTCTGCGCGTTCGGCGGCCAGGGCCGGGTGCTG
GCCGCCTACCGCGAGGCGGTCGCGCGCGGCTACCGCTTCTTCAGCTACGGGGACGCGATGCTCCTGCTCCGCCGCTGA

Upstream 100 bases:

>100_bases
TGGGGCGCGGCCGGGATGGCGCGCGAGGGGAGGGACTACCGCGAGATCCTCCGCCACTACTACCCGGGCACCGACGTGCT
GCGCATGTACTGACCCGACC

Downstream 100 bases:

>100_bases
GCCCGGCGCGTCCCGTCCGGCGCGCCGTGAGGTGCGCCGGTGTGTGCTATACGGGCGGCGAGGAGCGGGGGGTTTGCACA
GCGTCTCGGTCAACGTGGAG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRLSDFDFALPEGLVAQAPVTPRDASRLMVLAPEEGSPAHRGFADLPELLAPGDLLVFNDTRVIPARLLGHKASGGKVEL
LLCEPLEGGLGRRWRAMGQASKPIREGAVLTFDGLEARVDDVEGEGFYRVTLDRQGPELEAALGRAGRIPLPPYIRRAPD
AEDAARYQTIWARAPGSAAAPTAGLHFTEPLLARLAARGIRRTAVTLHVGPGTFLPIRGDDLDLHRMHGERYEVSPAAAA
ELAATRARGGRIVAVGTTSVRTLESAWRDGAVAAGPGRTELFIRPGHPFHAVDAMVTNFHLPRSTLLVLVCAFGGQGRVL
AAYREAVARGYRFFSYGDAMLLLRR

Sequences:

>Translated_345_residues
MRLSDFDFALPEGLVAQAPVTPRDASRLMVLAPEEGSPAHRGFADLPELLAPGDLLVFNDTRVIPARLLGHKASGGKVEL
LLCEPLEGGLGRRWRAMGQASKPIREGAVLTFDGLEARVDDVEGEGFYRVTLDRQGPELEAALGRAGRIPLPPYIRRAPD
AEDAARYQTIWARAPGSAAAPTAGLHFTEPLLARLAARGIRRTAVTLHVGPGTFLPIRGDDLDLHRMHGERYEVSPAAAA
ELAATRARGGRIVAVGTTSVRTLESAWRDGAVAAGPGRTELFIRPGHPFHAVDAMVTNFHLPRSTLLVLVCAFGGQGRVL
AAYREAVARGYRFFSYGDAMLLLRR
>Mature_345_residues
MRLSDFDFALPEGLVAQAPVTPRDASRLMVLAPEEGSPAHRGFADLPELLAPGDLLVFNDTRVIPARLLGHKASGGKVEL
LLCEPLEGGLGRRWRAMGQASKPIREGAVLTFDGLEARVDDVEGEGFYRVTLDRQGPELEAALGRAGRIPLPPYIRRAPD
AEDAARYQTIWARAPGSAAAPTAGLHFTEPLLARLAARGIRRTAVTLHVGPGTFLPIRGDDLDLHRMHGERYEVSPAAAA
ELAATRARGGRIVAVGTTSVRTLESAWRDGAVAAGPGRTELFIRPGHPFHAVDAMVTNFHLPRSTLLVLVCAFGGQGRVL
AAYREAVARGYRFFSYGDAMLLLRR

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=348, Percent_Identity=46.551724137931, Blast_Score=291, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_ANAD2 (B8JH48)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491816.1
- ProteinModelPortal:   B8JH48
- SMR:   B8JH48
- GeneID:   7299927
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1406
- HOGENOM:   HBG286076
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37183; Mature: 37183

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSDFDFALPEGLVAQAPVTPRDASRLMVLAPEEGSPAHRGFADLPELLAPGDLLVFND
CCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEECC
TRVIPARLLGHKASGGKVELLLCEPLEGGLGRRWRAMGQASKPIREGAVLTFDGLEARVD
CCEEHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCHHCCCEEEECCCCEEEE
DVEGEGFYRVTLDRQGPELEAALGRAGRIPLPPYIRRAPDAEDAARYQTIWARAPGSAAA
CCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHEEEEECCCCCCC
PTAGLHFTEPLLARLAARGIRRTAVTLHVGPGTFLPIRGDDLDLHRMHGERYEVSPAAAA
CCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCEEEEECCCCCHHHHHCCCEEECCHHHHH
ELAATRARGGRIVAVGTTSVRTLESAWRDGAVAAGPGRTELFIRPGHPFHAVDAMVTNFH
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHHHHHCC
LPRSTLLVLVCAFGGQGRVLAAYREAVARGYRFFSYGDAMLLLRR
CCHHHEEEEEEECCCCCCCHHHHHHHHHHCCEEEEECCEEEEEEC
>Mature Secondary Structure
MRLSDFDFALPEGLVAQAPVTPRDASRLMVLAPEEGSPAHRGFADLPELLAPGDLLVFND
CCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEECC
TRVIPARLLGHKASGGKVELLLCEPLEGGLGRRWRAMGQASKPIREGAVLTFDGLEARVD
CCEEHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCHHCCCEEEECCCCEEEE
DVEGEGFYRVTLDRQGPELEAALGRAGRIPLPPYIRRAPDAEDAARYQTIWARAPGSAAA
CCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHEEEEECCCCCCC
PTAGLHFTEPLLARLAARGIRRTAVTLHVGPGTFLPIRGDDLDLHRMHGERYEVSPAAAA
CCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCEEEEECCCCCHHHHHCCCEEECCHHHHH
ELAATRARGGRIVAVGTTSVRTLESAWRDGAVAAGPGRTELFIRPGHPFHAVDAMVTNFH
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHHHHHCC
LPRSTLLVLVCAFGGQGRVLAAYREAVARGYRFFSYGDAMLLLRR
CCHHHEEEEEEECCCCCCCHHHHHHHHHHCCEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA