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The map label for this gene is acnB [H]

Identifier: 220916447

GI number: 220916447

Start: 1492304

End: 1494853

Strand: Direct

Name: acnB [H]

Synonym: A2cp1_1341

Alternate gene names: 220916447

Gene position: 1492304-1494853 (Clockwise)

Preceding gene: 220916446

Following gene: 220916449

Centisome position: 29.67

GC content: 69.84

Gene sequence:

>2550_bases
GTGATCGAGGCGTACCTGAAGCACGAGGCCGAGCGGAAGGCCCAGGGGATCCCGCCGAAGCCCCTCGACCCCGAGCAGAC
GAAGGGGCTGGTCAAGCTCCTCGAGGCGCCGCCGAAGGGCCAGGAGGCGTTCCTCCTCGGGCTGCTGCGCGACCGCGTGT
CGCCCGGCGTGGATCCGGCCGCCGAGGTGAAGGCGAAGTTCCTCGCCGACGTGGTCTCCGGCGCGAAGACGTGCCCGCTC
GTCTCGCGCGCCCAGGCGGTGGAGCTGCTCGGCACGATGATGGGCGGCTACAACGTGCCGCCGCTGGTCGCCGCGCTGAA
GGACCCGGAGCTGGCCGCCGCGGCCGCGAAGGCGCTCTCGCACACCACCTACGTCTACGGCGCGTTCGACGACGTGCTGG
CGCTCTCGAAGACGAACGCCGCCGCGAAGTCGGTGGTGGACGCGTGGGCCAAGGCCGAGTGGTTCTCGTCGCGCCCCGGC
GTGCCCGAGACCATCAAGGTCAAGGTCTTCAAGGTGGACGGCGAGATCAACACCGACGACTTCTCGCCCGCCGGCGACGC
CTCCACCCGTCCGGACATCCCGCTCCACGCGCTGGCCATGGGCAAGACCCGCTTCCCGGGCGGCCTGAAGACCATCGCGG
AGTTCCGCGCCCAGGGCTTCCAGGTCGCGTTCGTGGGCGACGTGGTGGGCACCGGCTCCTCCCGCAAGTCGGCCTGCAAC
TCGGTGCTCTGGGCCATCGGCGAGGACATCCCGTTCGTGCCGAACAAGCGCCGCGGCGGCGTGATCATCGGCGGCGTCAT
CGCCCCGATCTTCTTCAACACCGCGCAGGACTCCGGCGCGCTGCCCATCCGCACCGACGTCACCAAGCTGAAGACCGGCG
ACGTGGTGGTGATCGACACGAAGAAGGGCGAGGTCCGCACCGAGCAGGGCGAGGTGCTCTCCACCTTCAAGCTCGAGCCG
GTCACCGTGCCGGACGAGTTCCGCGCCGGCGGCCGCATCCCGCTCATCATCGGGCGCGCGCTCACCGACAAGGCGCGCAA
GGCGCTCGGGCTGAAGGAGATCGACCTGTTCGCCGTCGCGAAGAACCCGCAGCCGAAGGCCGGCCAGGGCTACTCGCTCG
CGCAGAAGATGGTCGGCAAGGCGTGCGGCATCGCCGGCGCGCTGCCCGGCGGCTACTACGAGCCGAGGATGACCACGGTC
GGCTCGCAGGACACCACCGGCCCCATGACCGCCGACGAGCTGAAGGAGCTCGCCTGCCTGAAGTTCCAGGCGCCCATGGT
GATGCAGTCGTTCTGCCACACCGCGGCGTACCCGAAGCCGGCCGACGTGAAGATGCACGGCTCGCTGCCGTCCTTCATCC
AGCAGCGCGGCGGCGTGGCGCTCCGCCCCGGCGACGGCGTGATCCACTCGTGGCTGAACCGCCTGCTGCTCCCCGACACG
GTGGGCACCGGCGGCGACTCGCACACCCGCTTCCCCATCGGCATCTCCTTCCCGGCCGGCTCCGGCCTGGTGGCGTTCGG
CGCGGCGCTCGGCTTCATGCCGCTCGACATGCCGGAGAGCGTGCTGGTGCGGTTCAAGGGCAAGCTCAACCCGGGCATCA
CGCTCCGTGACGTGGTGAACGCGATCCCGTACTGGGCCATCAAGCAGGGCCTGCTGACCGTCCCGAAGAAGAACAAGAAG
AACGTGTTCAACGGCCGGATCCTGGAGATGGAGGGCCTGCCCGACCTGACCGTGGAGCAGGCGTTCGAGCTGACCGACGC
GGCGGCGGAGCGCAGCGCCGCGGCGGCCGCGGTGGACCTCTCGGAGAAGAGCGTCGCGACGTACCTCCGCTCGAACGTGG
CGCTGATGCGGAAGATGATCGCCGACGGGTACGGCGACAAGGCGGCGCTGGAGGCGCGCATCGCGGCGGTGGAGGCCTGG
CTCGCGAAGCCGGAGCTGCTCCGCGCCGACAGGAACGCCGACTACGCCGCGGTGATCGAGATCGACTTGGCCGAGATCAC
CGAGCCGATCCTGGCGTGCCCGAACGACCCGGACGACGTGAAGCTGCTGTCGGACGTCCAGAACACGAAGATCGACGACG
TGTTCCTGGGCTCCTGCATGACCAACATCGGCCACTTCCGCGCGGCCGGCGAGATCTGGAAGGGCCAGAAGAACACCGGC
CTGGTCCGCACCTGGCTCTGCCCGCCCACGCGGATGGACCAGCAGGAGCTGCGCGACGAGGCGTACTTCTCCGTCTACAG
CCAGATCGGCGCCCGCATCGAGATCGCCGGCTGCTCGCTGTGCATGGGCAACCAGGCGCGCGTGCCGGACGGCGTGAACG
TGTACTCGACGTCCACCCGCAACTTCGACGACCGCATGGGCAACGGCGCCCGCGTGTTCCTCGGCTCCGCCGAGCTGGGC
GCGGTGGCGGCGAACCTGGGGCGGCTGCCGACGGTGGCCGAGTACTTCGCCGTCTACAACGAGAAGATCGCGCCCAAGGC
CGGCCAGGTGTACCGGTACCTGCAGTTCGACGAGCTCGCCGAGTACAAGGGCTTCCGCGTCGTCGCGTAG

Upstream 100 bases:

>100_bases
GTTCGGCGGGTGGGCGCGGCTGACACCCGCATCCGGTCGTGCTATACGGCATCGCTTTTTCCGATCTTTCCACCCGCACC
AGTTCCGAAGGAGGAGCCCT

Downstream 100 bases:

>100_bases
CGCACGGCCGCGCCGTCCTCGAGGCCCCTCTCCCGCGCCGCGGGGGAGGGGCCTCCTCGTTGGCGCGCGGCGGGGGCCTC
GCGCCCTCTGGGCCGCCAGG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 849; Mature: 849

Protein sequence:

>849_residues
MIEAYLKHEAERKAQGIPPKPLDPEQTKGLVKLLEAPPKGQEAFLLGLLRDRVSPGVDPAAEVKAKFLADVVSGAKTCPL
VSRAQAVELLGTMMGGYNVPPLVAALKDPELAAAAAKALSHTTYVYGAFDDVLALSKTNAAAKSVVDAWAKAEWFSSRPG
VPETIKVKVFKVDGEINTDDFSPAGDASTRPDIPLHALAMGKTRFPGGLKTIAEFRAQGFQVAFVGDVVGTGSSRKSACN
SVLWAIGEDIPFVPNKRRGGVIIGGVIAPIFFNTAQDSGALPIRTDVTKLKTGDVVVIDTKKGEVRTEQGEVLSTFKLEP
VTVPDEFRAGGRIPLIIGRALTDKARKALGLKEIDLFAVAKNPQPKAGQGYSLAQKMVGKACGIAGALPGGYYEPRMTTV
GSQDTTGPMTADELKELACLKFQAPMVMQSFCHTAAYPKPADVKMHGSLPSFIQQRGGVALRPGDGVIHSWLNRLLLPDT
VGTGGDSHTRFPIGISFPAGSGLVAFGAALGFMPLDMPESVLVRFKGKLNPGITLRDVVNAIPYWAIKQGLLTVPKKNKK
NVFNGRILEMEGLPDLTVEQAFELTDAAAERSAAAAAVDLSEKSVATYLRSNVALMRKMIADGYGDKAALEARIAAVEAW
LAKPELLRADRNADYAAVIEIDLAEITEPILACPNDPDDVKLLSDVQNTKIDDVFLGSCMTNIGHFRAAGEIWKGQKNTG
LVRTWLCPPTRMDQQELRDEAYFSVYSQIGARIEIAGCSLCMGNQARVPDGVNVYSTSTRNFDDRMGNGARVFLGSAELG
AVAANLGRLPTVAEYFAVYNEKIAPKAGQVYRYLQFDELAEYKGFRVVA

Sequences:

>Translated_849_residues
MIEAYLKHEAERKAQGIPPKPLDPEQTKGLVKLLEAPPKGQEAFLLGLLRDRVSPGVDPAAEVKAKFLADVVSGAKTCPL
VSRAQAVELLGTMMGGYNVPPLVAALKDPELAAAAAKALSHTTYVYGAFDDVLALSKTNAAAKSVVDAWAKAEWFSSRPG
VPETIKVKVFKVDGEINTDDFSPAGDASTRPDIPLHALAMGKTRFPGGLKTIAEFRAQGFQVAFVGDVVGTGSSRKSACN
SVLWAIGEDIPFVPNKRRGGVIIGGVIAPIFFNTAQDSGALPIRTDVTKLKTGDVVVIDTKKGEVRTEQGEVLSTFKLEP
VTVPDEFRAGGRIPLIIGRALTDKARKALGLKEIDLFAVAKNPQPKAGQGYSLAQKMVGKACGIAGALPGGYYEPRMTTV
GSQDTTGPMTADELKELACLKFQAPMVMQSFCHTAAYPKPADVKMHGSLPSFIQQRGGVALRPGDGVIHSWLNRLLLPDT
VGTGGDSHTRFPIGISFPAGSGLVAFGAALGFMPLDMPESVLVRFKGKLNPGITLRDVVNAIPYWAIKQGLLTVPKKNKK
NVFNGRILEMEGLPDLTVEQAFELTDAAAERSAAAAAVDLSEKSVATYLRSNVALMRKMIADGYGDKAALEARIAAVEAW
LAKPELLRADRNADYAAVIEIDLAEITEPILACPNDPDDVKLLSDVQNTKIDDVFLGSCMTNIGHFRAAGEIWKGQKNTG
LVRTWLCPPTRMDQQELRDEAYFSVYSQIGARIEIAGCSLCMGNQARVPDGVNVYSTSTRNFDDRMGNGARVFLGSAELG
AVAANLGRLPTVAEYFAVYNEKIAPKAGQVYRYLQFDELAEYKGFRVVA
>Mature_849_residues
MIEAYLKHEAERKAQGIPPKPLDPEQTKGLVKLLEAPPKGQEAFLLGLLRDRVSPGVDPAAEVKAKFLADVVSGAKTCPL
VSRAQAVELLGTMMGGYNVPPLVAALKDPELAAAAAKALSHTTYVYGAFDDVLALSKTNAAAKSVVDAWAKAEWFSSRPG
VPETIKVKVFKVDGEINTDDFSPAGDASTRPDIPLHALAMGKTRFPGGLKTIAEFRAQGFQVAFVGDVVGTGSSRKSACN
SVLWAIGEDIPFVPNKRRGGVIIGGVIAPIFFNTAQDSGALPIRTDVTKLKTGDVVVIDTKKGEVRTEQGEVLSTFKLEP
VTVPDEFRAGGRIPLIIGRALTDKARKALGLKEIDLFAVAKNPQPKAGQGYSLAQKMVGKACGIAGALPGGYYEPRMTTV
GSQDTTGPMTADELKELACLKFQAPMVMQSFCHTAAYPKPADVKMHGSLPSFIQQRGGVALRPGDGVIHSWLNRLLLPDT
VGTGGDSHTRFPIGISFPAGSGLVAFGAALGFMPLDMPESVLVRFKGKLNPGITLRDVVNAIPYWAIKQGLLTVPKKNKK
NVFNGRILEMEGLPDLTVEQAFELTDAAAERSAAAAAVDLSEKSVATYLRSNVALMRKMIADGYGDKAALEARIAAVEAW
LAKPELLRADRNADYAAVIEIDLAEITEPILACPNDPDDVKLLSDVQNTKIDDVFLGSCMTNIGHFRAAGEIWKGQKNTG
LVRTWLCPPTRMDQQELRDEAYFSVYSQIGARIEIAGCSLCMGNQARVPDGVNVYSTSTRNFDDRMGNGARVFLGSAELG
AVAANLGRLPTVAEYFAVYNEKIAPKAGQVYRYLQFDELAEYKGFRVVA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=854, Percent_Identity=63.3489461358314, Blast_Score=1040, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=374, Percent_Identity=25.4010695187166, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 90980; Mature: 90980

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEAYLKHEAERKAQGIPPKPLDPEQTKGLVKLLEAPPKGQEAFLLGLLRDRVSPGVDPA
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHH
AEVKAKFLADVVSGAKTCPLVSRAQAVELLGTMMGGYNVPPLVAALKDPELAAAAAKALS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHH
HTTYVYGAFDDVLALSKTNAAAKSVVDAWAKAEWFSSRPGVPETIKVKVFKVDGEINTDD
CCEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCC
FSPAGDASTRPDIPLHALAMGKTRFPGGLKTIAEFRAQGFQVAFVGDVVGTGSSRKSACN
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHH
SVLWAIGEDIPFVPNKRRGGVIIGGVIAPIFFNTAQDSGALPIRTDVTKLKTGDVVVIDT
HHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCEECCHHEECCCCEEEEEC
KKGEVRTEQGEVLSTFKLEPVTVPDEFRAGGRIPLIIGRALTDKARKALGLKEIDLFAVA
CCCCCCCCCCCEEEEEEECCCCCCHHHCCCCCCEEEECHHHHHHHHHHCCCCEEEEEEEE
KNPQPKAGQGYSLAQKMVGKACGIAGALPGGYYEPRMTTVGSQDTTGPMTADELKELACL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
KFQAPMVMQSFCHTAAYPKPADVKMHGSLPSFIQQRGGVALRPGDGVIHSWLNRLLLPDT
HHCCHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHCCCEEECCCCCHHHHHHHHHCCCCC
VGTGGDSHTRFPIGISFPAGSGLVAFGAALGFMPLDMPESVLVRFKGKLNPGITLRDVVN
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHEECCCCCCCEEHHHHHH
AIPYWAIKQGLLTVPKKNKKNVFNGRILEMEGLPDLTVEQAFELTDAAAERSAAAAAVDL
HCCHHHHHHCCEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEECC
SEKSVATYLRSNVALMRKMIADGYGDKAALEARIAAVEAWLAKPELLRADRNADYAAVIE
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEE
IDLAEITEPILACPNDPDDVKLLSDVQNTKIDDVFLGSCMTNIGHFRAAGEIWKGQKNTG
EEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LVRTWLCPPTRMDQQELRDEAYFSVYSQIGARIEIAGCSLCMGNQARVPDGVNVYSTSTR
EEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCEEEECCCC
NFDDRMGNGARVFLGSAELGAVAANLGRLPTVAEYFAVYNEKIAPKAGQVYRYLQFDELA
CHHHHCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
EYKGFRVVA
HCCCCEEEC
>Mature Secondary Structure
MIEAYLKHEAERKAQGIPPKPLDPEQTKGLVKLLEAPPKGQEAFLLGLLRDRVSPGVDPA
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHH
AEVKAKFLADVVSGAKTCPLVSRAQAVELLGTMMGGYNVPPLVAALKDPELAAAAAKALS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHH
HTTYVYGAFDDVLALSKTNAAAKSVVDAWAKAEWFSSRPGVPETIKVKVFKVDGEINTDD
CCEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCC
FSPAGDASTRPDIPLHALAMGKTRFPGGLKTIAEFRAQGFQVAFVGDVVGTGSSRKSACN
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHH
SVLWAIGEDIPFVPNKRRGGVIIGGVIAPIFFNTAQDSGALPIRTDVTKLKTGDVVVIDT
HHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCEECCHHEECCCCEEEEEC
KKGEVRTEQGEVLSTFKLEPVTVPDEFRAGGRIPLIIGRALTDKARKALGLKEIDLFAVA
CCCCCCCCCCCEEEEEEECCCCCCHHHCCCCCCEEEECHHHHHHHHHHCCCCEEEEEEEE
KNPQPKAGQGYSLAQKMVGKACGIAGALPGGYYEPRMTTVGSQDTTGPMTADELKELACL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
KFQAPMVMQSFCHTAAYPKPADVKMHGSLPSFIQQRGGVALRPGDGVIHSWLNRLLLPDT
HHCCHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHCCCEEECCCCCHHHHHHHHHCCCCC
VGTGGDSHTRFPIGISFPAGSGLVAFGAALGFMPLDMPESVLVRFKGKLNPGITLRDVVN
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHEECCCCCCCEEHHHHHH
AIPYWAIKQGLLTVPKKNKKNVFNGRILEMEGLPDLTVEQAFELTDAAAERSAAAAAVDL
HCCHHHHHHCCEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEECC
SEKSVATYLRSNVALMRKMIADGYGDKAALEARIAAVEAWLAKPELLRADRNADYAAVIE
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEE
IDLAEITEPILACPNDPDDVKLLSDVQNTKIDDVFLGSCMTNIGHFRAAGEIWKGQKNTG
EEHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LVRTWLCPPTRMDQQELRDEAYFSVYSQIGARIEIAGCSLCMGNQARVPDGVNVYSTSTR
EEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCEEEECCCC
NFDDRMGNGARVFLGSAELGAVAANLGRLPTVAEYFAVYNEKIAPKAGQVYRYLQFDELA
CHHHHCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
EYKGFRVVA
HCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]