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The map label for this gene is trmJ [H]

Identifier: 220916347

GI number: 220916347

Start: 1393119

End: 1393928

Strand: Direct

Name: trmJ [H]

Synonym: A2cp1_1241

Alternate gene names: 220916347

Gene position: 1393119-1393928 (Clockwise)

Preceding gene: 220916344

Following gene: 220916359

Centisome position: 27.7

GC content: 78.02

Gene sequence:

>810_bases
ATGCACCCGGGAAAAATCCGCCTCGTGCTCCACCGGCCGCAGAGCGCCGACAACGTCGGCGCCGCCGCCCGGGTGATGAA
GAACTTCGGCCTGTCCCGCCTGGTGGTGGTGGCCCCGGCGGCCTGGGGCGGGCCGCCGCGCACCCCCGGAACGGGCGCGG
TCACCGCCCGCGACGACGTGCTCGCCCGCGCGCGACGGATGGCCCGGAAGGCCTCGGACCTGCTCGACGGGGCCGAGATC
CACCCCGACCTCCGCTCGGCCATCGGCCCGGCGACCTGGGTGTGCGGCACGACCTCCCGCGCGGTGGAGGGGCGGCCCTG
GCTCGAGCCGCGCGGCCTGGCGGCCGAGCTGGTCCGGCGCTCCGAGCAGGGCGAGGTGGCGGTGGTGTTCGGCGAGGAGC
GCCGCGGGCTGTCCGACGCCGAGCTGGAGCTGTGCCAGGCCGTCTGCACCATCCCCACGGCCGCCGCCTACGACTCGATG
AACCTGGCGCAGGCGGTGGCAGTGGTCGCGTACGAGGTGGCGCGCGCGGCGCCGCCGGCCGGCGCGGCGCCCGCGGAGGC
GCCGCCCGGACGCGGCGGCGATGCGCCGGCCCGCATCGAGACCGTGGAGGCGCTGTGGGCGCGCGCGCAGGCGGTCCTCG
GCGCCGCGGGGTACCTGAACCCGCAGAACCCGGAGCACATCCTGGCGGACTTCCGGCGGCTGCTCGCGCGCGCCGACCCG
ACGCAGCGCGAGGTCGAGCTGCTCCTCGGGGCGCTGCGATCGCTGGAGCGTGCGCTGCGGCTGCCGCCGCGCGGCGACGG
AACGGCCTAG

Upstream 100 bases:

>100_bases
CCGAGACCGGGGAGGTCGAGCTGCTGGAGGGTGTGCGGGAGCTGGGCCCGCAGCTTGTCGTCGGGGATCATGGGCGGGAT
GTTATACGTGGCGGGCGCAC

Downstream 100 bases:

>100_bases
GCCGCGGGAGCCGCCTCGGCGCCCTGGTTCTTCTTGCGCGACTTCTTCCACGCGGTGAAGTGCTCCTCGCACAGGCCGTG
CTGGAACACCTTCTTCTTGC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MHPGKIRLVLHRPQSADNVGAAARVMKNFGLSRLVVVAPAAWGGPPRTPGTGAVTARDDVLARARRMARKASDLLDGAEI
HPDLRSAIGPATWVCGTTSRAVEGRPWLEPRGLAAELVRRSEQGEVAVVFGEERRGLSDAELELCQAVCTIPTAAAYDSM
NLAQAVAVVAYEVARAAPPAGAAPAEAPPGRGGDAPARIETVEALWARAQAVLGAAGYLNPQNPEHILADFRRLLARADP
TQREVELLLGALRSLERALRLPPRGDGTA

Sequences:

>Translated_269_residues
MHPGKIRLVLHRPQSADNVGAAARVMKNFGLSRLVVVAPAAWGGPPRTPGTGAVTARDDVLARARRMARKASDLLDGAEI
HPDLRSAIGPATWVCGTTSRAVEGRPWLEPRGLAAELVRRSEQGEVAVVFGEERRGLSDAELELCQAVCTIPTAAAYDSM
NLAQAVAVVAYEVARAAPPAGAAPAEAPPGRGGDAPARIETVEALWARAQAVLGAAGYLNPQNPEHILADFRRLLARADP
TQREVELLLGALRSLERALRLPPRGDGTA
>Mature_269_residues
MHPGKIRLVLHRPQSADNVGAAARVMKNFGLSRLVVVAPAAWGGPPRTPGTGAVTARDDVLARARRMARKASDLLDGAEI
HPDLRSAIGPATWVCGTTSRAVEGRPWLEPRGLAAELVRRSEQGEVAVVFGEERRGLSDAELELCQAVCTIPTAAAYDSM
NLAQAVAVVAYEVARAAPPAGAAPAEAPPGRGGDAPARIETVEALWARAQAVLGAAGYLNPQNPEHILADFRRLLARADP
TQREVELLLGALRSLERALRLPPRGDGTA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=255, Percent_Identity=32.156862745098, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790865, Length=219, Percent_Identity=29.6803652968037, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28375; Mature: 28375

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPGKIRLVLHRPQSADNVGAAARVMKNFGLSRLVVVAPAAWGGPPRTPGTGAVTARDDV
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHH
LARARRMARKASDLLDGAEIHPDLRSAIGPATWVCGTTSRAVEGRPWLEPRGLAAELVRR
HHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHC
SEQGEVAVVFGEERRGLSDAELELCQAVCTIPTAAAYDSMNLAQAVAVVAYEVARAAPPA
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAAPAEAPPGRGGDAPARIETVEALWARAQAVLGAAGYLNPQNPEHILADFRRLLARADP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
TQREVELLLGALRSLERALRLPPRGDGTA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHPGKIRLVLHRPQSADNVGAAARVMKNFGLSRLVVVAPAAWGGPPRTPGTGAVTARDDV
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHH
LARARRMARKASDLLDGAEIHPDLRSAIGPATWVCGTTSRAVEGRPWLEPRGLAAELVRR
HHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHC
SEQGEVAVVFGEERRGLSDAELELCQAVCTIPTAAAYDSMNLAQAVAVVAYEVARAAPPA
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAAPAEAPPGRGGDAPARIETVEALWARAQAVLGAAGYLNPQNPEHILADFRRLLARADP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
TQREVELLLGALRSLERALRLPPRGDGTA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA