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The map label for this gene is nfo [H]

Identifier: 220916284

GI number: 220916284

Start: 1315759

End: 1316586

Strand: Reverse

Name: nfo [H]

Synonym: A2cp1_1177

Alternate gene names: 220916284

Gene position: 1316586-1315759 (Counterclockwise)

Preceding gene: 220916285

Following gene: 220916283

Centisome position: 26.18

GC content: 71.62

Gene sequence:

>828_bases
ATGCTCCTGGGAGCACACGAAGGCATCGCCGGTGGGGTCTCGACCGCGTTCGCGCGCGCCGAGGCCGACGGCGCCGACTG
CCTGCAGATCTTCACCCGCAACGCCCGCGGCTGGGCGGCGAAGCCGCTCGAGCCCGACGAGGTGAAGCGCTTCCAGGGCG
AGGCCCGCCGTACCCGCAAGCCGGTGGCGGCCCACTCCTCCTACCTCATCAACAGCGCCGCGGCCGACCGCGACCTGCGG
AAGAAGAGCTGGGACGCGCTCGCCGACGAGCTGGACCGCTGCGAGCGGCTCGGCATCCCGGGGCTGATCTTCCACCCGGG
GAGCCACGAGAACGCGGCCCAGGGCCTCGAGCTCGTGGCCGAGGGGATGCAGCGGGCCATCGAGAAGGTCCCCGGCAAGG
CGAAGCTCCTCGTCGAGACCACCGCCGGCCAGGGATCGAGCCTGGGCTGGCGGTTCGAGGAGATCGCCGCCATCCGCCAG
GCGATCCCCGGCGCGGCCCGCCGCCGCACCGGCGTCTGCGTGGACACCTGCCACCTGTTCGCGGCGGGCTACGATCTCAC
CACCGAGGAGGGATACCACCGCACCTTCCAGGAGCTCGACCGCGTGGTCGGCCTCTCGAACGTGCGCGCGTTCCACCTCA
ACGACTCGAAGAAGCCGCTCGGGTGCCGGGTGGACCGGCACGAGCACATCGGGCAGGGCGCGATGGGGCTGGACCCCTTC
CGCCGCCTGGTGAACGACCCCCGCTTCGCGGAGATCCCGGGGTTCGTGGAGACCGAGTCGCGATTCAAGGAGAACATCGA
GGTCCTCCGCGGCCTCGTACGCCGATGA

Upstream 100 bases:

>100_bases
ACGCAAGCTGCCGACACGACTGGATTTTGGGGCTGTCCCCGCCCGCGGCCGACCGAGCATGGCGGGGCGGGGGCCGGCTA
TTGTATAGCTACGCCCCCCT

Downstream 100 bases:

>100_bases
AGGTCCACCTTCCCATGCGCCAGCCGCCCGACGAGCCGCAGAAGCCGCGGATCCGCCCGGAGGTCCGCGCCGCCCGGATG
ACGCTCTATCCCCGCGGCGG

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLLGAHEGIAGGVSTAFARAEADGADCLQIFTRNARGWAAKPLEPDEVKRFQGEARRTRKPVAAHSSYLINSAAADRDLR
KKSWDALADELDRCERLGIPGLIFHPGSHENAAQGLELVAEGMQRAIEKVPGKAKLLVETTAGQGSSLGWRFEEIAAIRQ
AIPGAARRRTGVCVDTCHLFAAGYDLTTEEGYHRTFQELDRVVGLSNVRAFHLNDSKKPLGCRVDRHEHIGQGAMGLDPF
RRLVNDPRFAEIPGFVETESRFKENIEVLRGLVRR

Sequences:

>Translated_275_residues
MLLGAHEGIAGGVSTAFARAEADGADCLQIFTRNARGWAAKPLEPDEVKRFQGEARRTRKPVAAHSSYLINSAAADRDLR
KKSWDALADELDRCERLGIPGLIFHPGSHENAAQGLELVAEGMQRAIEKVPGKAKLLVETTAGQGSSLGWRFEEIAAIRQ
AIPGAARRRTGVCVDTCHLFAAGYDLTTEEGYHRTFQELDRVVGLSNVRAFHLNDSKKPLGCRVDRHEHIGQGAMGLDPF
RRLVNDPRFAEIPGFVETESRFKENIEVLRGLVRR
>Mature_275_residues
MLLGAHEGIAGGVSTAFARAEADGADCLQIFTRNARGWAAKPLEPDEVKRFQGEARRTRKPVAAHSSYLINSAAADRDLR
KKSWDALADELDRCERLGIPGLIFHPGSHENAAQGLELVAEGMQRAIEKVPGKAKLLVETTAGQGSSLGWRFEEIAAIRQ
AIPGAARRRTGVCVDTCHLFAAGYDLTTEEGYHRTFQELDRVVGLSNVRAFHLNDSKKPLGCRVDRHEHIGQGAMGLDPF
RRLVNDPRFAEIPGFVETESRFKENIEVLRGLVRR

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=262, Percent_Identity=41.9847328244275, Blast_Score=201, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17531193, Length=281, Percent_Identity=37.7224199288256, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322735, Length=262, Percent_Identity=35.4961832061069, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 30304; Mature: 30304

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGAHEGIAGGVSTAFARAEADGADCLQIFTRNARGWAAKPLEPDEVKRFQGEARRTRK
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
PVAAHSSYLINSAAADRDLRKKSWDALADELDRCERLGIPGLIFHPGSHENAAQGLELVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
EGMQRAIEKVPGKAKLLVETTAGQGSSLGWRFEEIAAIRQAIPGAARRRTGVCVDTCHLF
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHH
AAGYDLTTEEGYHRTFQELDRVVGLSNVRAFHLNDSKKPLGCRVDRHEHIGQGAMGLDPF
HHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHH
RRLVNDPRFAEIPGFVETESRFKENIEVLRGLVRR
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLGAHEGIAGGVSTAFARAEADGADCLQIFTRNARGWAAKPLEPDEVKRFQGEARRTRK
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
PVAAHSSYLINSAAADRDLRKKSWDALADELDRCERLGIPGLIFHPGSHENAAQGLELVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
EGMQRAIEKVPGKAKLLVETTAGQGSSLGWRFEEIAAIRQAIPGAARRRTGVCVDTCHLF
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHH
AAGYDLTTEEGYHRTFQELDRVVGLSNVRAFHLNDSKKPLGCRVDRHEHIGQGAMGLDPF
HHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHH
RRLVNDPRFAEIPGFVETESRFKENIEVLRGLVRR
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA