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The map label for this gene is iscS [H]

Identifier: 220916282

GI number: 220916282

Start: 1313461

End: 1314606

Strand: Reverse

Name: iscS [H]

Synonym: A2cp1_1175

Alternate gene names: 220916282

Gene position: 1314606-1313461 (Counterclockwise)

Preceding gene: 220916283

Following gene: 220916280

Centisome position: 26.14

GC content: 79.76

Gene sequence:

>1146_bases
GTGATCTACCTCGACCACAACGCCATCACGCCCATGCGGCCCGACGCCAGGGCCGCCGTGCGCGACGCGCTGGAGGTGTT
CGGCAACCCCAGCTCGGTCCACGCGGCCGGCCGCGCCGCGCGCGACCTGCTCGACTGCGCCCGGGACCGGGTGGCCCGCG
CGGTCGGGGCGGCCCCCGCCGAGATCGTGTTCACCTCCGGCGCCACCGAGTCGGCCGCGCTCGCGATCCGCGGCGTGCTC
GGGGCGGCGCCCGCCGGGCGTCGAAGGCTGGTGGTCACCGCGGTCGAGCACCCCTGCGTCCTCTCGCTCGCGCGCGCGCT
CGAGCGGGCCGGCACGCCGCTCACGGTGGTCCCGGTGGACCGCCGCGGCCAGGTGGACCCGGAGGCGTTCCGGGCCGCGC
TCGGGCCCGACGTCGCGCTCGCCTGCGCCATGCGCGCGAACAACGAGACCGGGGTGGTGCTGCCCGTGCCCGCGCTCGCC
GCGGCGGCGCGCGACGCGGGCGCGCCGTTCTTCTGCGACGCCGTGCAGGCGGCCGGGAAGATCCCGCTCGACGTGCGCGG
GCTGGGCGCGGACCTGGTGGCCCTCACCGGGCAGAAGTTCGGCGGGCCGCGCGGGGCCGGCGTGCTGTGGGTCGCGCCCG
GCCTGCGGCTCGCGCCGGTGCTGGGCGGCGAGCAGGAGCGCGGCCGGCGCGCCGGCACCGAGAACCTGCCCGGCATCGCC
GGCCTGGCCACCGCGCTGGAGGCGGCCGTCGCCGCCGTGGCGGAGGAGGGTCCGCGGCTGGCGGGGCTGCGCGACCGGCT
CGAGGCCGGGCTCCTCGCCGCGGTGCCGCGCGCCCGCGTGAACGGCGCCGGCGCCCCCCGCCTGCCCGGCACGCTCTCCA
TCACGTTCGAGGGCTGCGACGCCGAGGCGCTGCTCATGGCCATGGACCTCGAGGGCGTGTGCGCGAGCGCCGGGTCGGCC
TGCCACTCCGGCTCGACCCGGCCGTCGGGCGTGCTGCTCGCGCTCGGCCTCTCCGACGCGGACGCGCGCGCGACGCTGCG
CCTCTCGCTCGGCTGGACCACCACCGCCGAGGACGTGGACGCGGCGCTCCGGATCGTGCCGCCGCTCGTGGCGCGCGTGC
GCGGCGCCATCGCGGGCTCCGCGTAG

Upstream 100 bases:

>100_bases
GAGGAGGTCGTGAAGGACTTCCTCGACGCGTTCGGCGTGCGCGCCCGCAAGCCGAAGAAGCTCGTCTAGCCCTGCCCTGC
GGGCCGGCCGGAGCGCGCCG

Downstream 100 bases:

>100_bases
CGCGCCGCTACGCGGCCCCGAACAGCCCCCGCACCACCGCCGCGATCGCGAGCGGCGTGCCCACCGCCGGATCGTACAGC
GCTGCGATCTCGTCCGCGGC

Product: class V aminotransferase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MIYLDHNAITPMRPDARAAVRDALEVFGNPSSVHAAGRAARDLLDCARDRVARAVGAAPAEIVFTSGATESAALAIRGVL
GAAPAGRRRLVVTAVEHPCVLSLARALERAGTPLTVVPVDRRGQVDPEAFRAALGPDVALACAMRANNETGVVLPVPALA
AAARDAGAPFFCDAVQAAGKIPLDVRGLGADLVALTGQKFGGPRGAGVLWVAPGLRLAPVLGGEQERGRRAGTENLPGIA
GLATALEAAVAAVAEEGPRLAGLRDRLEAGLLAAVPRARVNGAGAPRLPGTLSITFEGCDAEALLMAMDLEGVCASAGSA
CHSGSTRPSGVLLALGLSDADARATLRLSLGWTTTAEDVDAALRIVPPLVARVRGAIAGSA

Sequences:

>Translated_381_residues
MIYLDHNAITPMRPDARAAVRDALEVFGNPSSVHAAGRAARDLLDCARDRVARAVGAAPAEIVFTSGATESAALAIRGVL
GAAPAGRRRLVVTAVEHPCVLSLARALERAGTPLTVVPVDRRGQVDPEAFRAALGPDVALACAMRANNETGVVLPVPALA
AAARDAGAPFFCDAVQAAGKIPLDVRGLGADLVALTGQKFGGPRGAGVLWVAPGLRLAPVLGGEQERGRRAGTENLPGIA
GLATALEAAVAAVAEEGPRLAGLRDRLEAGLLAAVPRARVNGAGAPRLPGTLSITFEGCDAEALLMAMDLEGVCASAGSA
CHSGSTRPSGVLLALGLSDADARATLRLSLGWTTTAEDVDAALRIVPPLVARVRGAIAGSA
>Mature_381_residues
MIYLDHNAITPMRPDARAAVRDALEVFGNPSSVHAAGRAARDLLDCARDRVARAVGAAPAEIVFTSGATESAALAIRGVL
GAAPAGRRRLVVTAVEHPCVLSLARALERAGTPLTVVPVDRRGQVDPEAFRAALGPDVALACAMRANNETGVVLPVPALA
AAARDAGAPFFCDAVQAAGKIPLDVRGLGADLVALTGQKFGGPRGAGVLWVAPGLRLAPVLGGEQERGRRAGTENLPGIA
GLATALEAAVAAVAEEGPRLAGLRDRLEAGLLAAVPRARVNGAGAPRLPGTLSITFEGCDAEALLMAMDLEGVCASAGSA
CHSGSTRPSGVLLALGLSDADARATLRLSLGWTTTAEDVDAALRIVPPLVARVRGAIAGSA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=378, Percent_Identity=37.5661375661376, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=35.5932203389831, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI48994898, Length=380, Percent_Identity=38.6842105263158, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1787970, Length=239, Percent_Identity=25.9414225941423, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789175, Length=395, Percent_Identity=26.8354430379747, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25143064, Length=379, Percent_Identity=37.2031662269129, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17533177, Length=317, Percent_Identity=35.0157728706625, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=378, Percent_Identity=37.3015873015873, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI20129463, Length=378, Percent_Identity=38.0952380952381, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 38469; Mature: 38469

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDHNAITPMRPDARAAVRDALEVFGNPSSVHAAGRAARDLLDCARDRVARAVGAAPA
CEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EIVFTSGATESAALAIRGVLGAAPAGRRRLVVTAVEHPCVLSLARALERAGTPLTVVPVD
EEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECC
RRGQVDPEAFRAALGPDVALACAMRANNETGVVLPVPALAAAARDAGAPFFCDAVQAAGK
CCCCCCHHHHHHHCCCCCEEEEEEECCCCCCEEEECHHHHHHHHCCCCCHHHHHHHHCCC
IPLDVRGLGADLVALTGQKFGGPRGAGVLWVAPGLRLAPVLGGEQERGRRAGTENLPGIA
CCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEEECCCCCHHHHCCCCCCCCCCHH
GLATALEAAVAAVAEEGPRLAGLRDRLEAGLLAAVPRARVNGAGAPRLPGTLSITFEGCD
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEEEEEECCC
AEALLMAMDLEGVCASAGSACHSGSTRPSGVLLALGLSDADARATLRLSLGWTTTAEDVD
CCEEEEEECCCHHHHHCCCHHCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHH
AALRIVPPLVARVRGAIAGSA
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIYLDHNAITPMRPDARAAVRDALEVFGNPSSVHAAGRAARDLLDCARDRVARAVGAAPA
CEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EIVFTSGATESAALAIRGVLGAAPAGRRRLVVTAVEHPCVLSLARALERAGTPLTVVPVD
EEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECC
RRGQVDPEAFRAALGPDVALACAMRANNETGVVLPVPALAAAARDAGAPFFCDAVQAAGK
CCCCCCHHHHHHHCCCCCEEEEEEECCCCCCEEEECHHHHHHHHCCCCCHHHHHHHHCCC
IPLDVRGLGADLVALTGQKFGGPRGAGVLWVAPGLRLAPVLGGEQERGRRAGTENLPGIA
CCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEEECCCCCHHHHCCCCCCCCCCHH
GLATALEAAVAAVAEEGPRLAGLRDRLEAGLLAAVPRARVNGAGAPRLPGTLSITFEGCD
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEEEEEECCC
AEALLMAMDLEGVCASAGSACHSGSTRPSGVLLALGLSDADARATLRLSLGWTTTAEDVD
CCEEEEEECCCHHHHHCCCHHCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCHHHHH
AALRIVPPLVARVRGAIAGSA
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]