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The map label for this gene is ilvG [H]

Identifier: 220916188

GI number: 220916188

Start: 1203341

End: 1205101

Strand: Reverse

Name: ilvG [H]

Synonym: A2cp1_1076

Alternate gene names: 220916188

Gene position: 1205101-1203341 (Counterclockwise)

Preceding gene: 220916189

Following gene: 220916187

Centisome position: 23.96

GC content: 76.72

Gene sequence:

>1761_bases
ATGGCGGAGGCCACGAACGGAGGCGCGGTGATCGCGGCGGCGCTGCAGCGCGCCGGTGTGGGCCACCTCTTCACGCTGTG
CGGCGGCCACATCTCGCCCATCCTCGTGGAGTGCAAGCGGCGCGGGCTTCGCGTGGTGGACGCGCGCGACGAGGCGAACG
CGGTGTTCGCGGCCGACGCCATGGCGCGCCTCACCGGCCGGCCCGGCGTGGCCGCGGTCACGGCGGGCCCCGGCGTCACG
AACGCCGTCACCGCGCTCGAGAACGCGCGGCTCGCGCAGTCGCCGGTGGTGCTCCTCGGCGGCGCGACCGCGACGCTGCT
CCGCGGCCGCGGCGCGCTGCAGGACATCGACCAGCTCGCCCTCATGCGCCCCATCGCCAAGTGGGCCACGCGCGTCACCA
CCGTCGGCGGGCTGCGCCCCACGCTCGAGCGCGCGCTCGCGCTGGCGCGCGGCGGCGTGCCGGGGCCGGTGTTCGTCGAG
GTCCCGGTGGACCTGCTCTACGACGAGGCGCTCGTCCGCGACCTGTACCGGCGCGAGTCGGGCGCCGACCGGGTGAGCGG
CGTCGCCGGCACGGCGCTGCGGCTCTACCTGGAGGCGCACCTCGCGCGGCAGTTCCGGGCGCCCGCGCTGCCCTCGCTCG
AGGACCTCCCGGCGCGGATCGCGGAGCGGATCGACCTGCGGCGAGGCACGTCGGGACGCGTCGAGCGGATCGCCGAGCGG
CTCTCGCGCGCGGAGCGTCCGGTGCTGATCCTCGGCAGCCAGGCGCTGGCGGGCTGCGAGGACCCTGAACGCCTCGCCGC
CGCGGTGCGCGCGCTCGGCGTGCCGGTCTACCTCGGCGGCATGGCGCGCGGCCTGCTCGGCCGGCGCGACGCCCTCCAGT
TCCGCCACGCGCGCGGGCGCGCGCTCAAGGAGGCGGACGTCGTCGTCGTGGCCGGGTTCCCGTTCGACTTCCGCCTCGGC
TACGGCCGCGGCTTCGGGCGCGGCGCGTTCATCGCGGCGGCGAACCTCTCCGCGGCCGAGCTCCGCAAGAACCGCAGCCC
CGACGTCGCGGTGGAGATGCACCCCGGGGAGCTCCTGGTGGCGCTGGCCGCGAAGGCGGGCAAGCCGCAGGCACGGGACG
CCTGGTTCGGCGCGCTCCGCGAGCGCGAGGCCGCGCGGGACCGCGAGATCGCCGCCGGCGCGGAGGCGACCGGCGAGCTC
GTCAACCCGCTGCGCTTCCTGCTCCGGCTCGAGGAGAAGATGGCCGAGGACGCGGCGCTGGTGGTGGACGGCGGTGACTT
CGTCGCGACGGCCGCCTACACGCTCCGCCCGCGCGCGCCGCTCGCCTGGCTCGACCCGGGCGTCTTCGGGACGCTCGGCG
TCGGCGGCGGGTTCGCGACCGCGGCCGCGCTCGCCCGGCCCGGGCGCGAGGTGTGGCTGCTCTACGGCGACGGCTCGTGC
GCCTACAGCCTCGCCGAGTTCGACACGTTCGCGCGCCACGGCCTCGCGCCCATCGCCGTGATCGGCAACGACGGCTCGTG
GCAGCAGATCGCGCGGGAGCAGGTGGACATGCTGGGCGACGACGTCGGGACGGTGCTCTCGCGCTGCGACTACCACCGCG
TCGCCGAGGGCTACGGCGGCGTCGGGCTGCTGCTGACCGAGGACGCCGGGATCGACGAGACCCTCGACCGCGCGCGGGAG
GCGGCGCGCGCCGGCCGGCCGGTCTGCATCAACGTCCACCTGCGCCGGAGCGAGTTCCGGAAGGGCTCGATCTCGATGTG
A

Upstream 100 bases:

>100_bases
CGACCCCCGGCCCCGTCGCGGGCCCACCGCTCGTCCCGCCCGGATGGTAGCGTCCGCAGCGAGAAGGTGGACGCCGCGGC
GCCCGCGGCCCGGAGGATCC

Downstream 100 bases:

>100_bases
GGGGGGCCGGGGGCGTCCTCACGCACCCCTGCCGCACCCGTCGCGCCCGCTGTTGCGCCTGTTCGAGGAGGCACGCTCCA
CCTGGGTTCACGGGCAGTGT

Product: thiamine pyrophosphate TPP-binding domain-containing protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MAEATNGGAVIAAALQRAGVGHLFTLCGGHISPILVECKRRGLRVVDARDEANAVFAADAMARLTGRPGVAAVTAGPGVT
NAVTALENARLAQSPVVLLGGATATLLRGRGALQDIDQLALMRPIAKWATRVTTVGGLRPTLERALALARGGVPGPVFVE
VPVDLLYDEALVRDLYRRESGADRVSGVAGTALRLYLEAHLARQFRAPALPSLEDLPARIAERIDLRRGTSGRVERIAER
LSRAERPVLILGSQALAGCEDPERLAAAVRALGVPVYLGGMARGLLGRRDALQFRHARGRALKEADVVVVAGFPFDFRLG
YGRGFGRGAFIAAANLSAAELRKNRSPDVAVEMHPGELLVALAAKAGKPQARDAWFGALREREAARDREIAAGAEATGEL
VNPLRFLLRLEEKMAEDAALVVDGGDFVATAAYTLRPRAPLAWLDPGVFGTLGVGGGFATAAALARPGREVWLLYGDGSC
AYSLAEFDTFARHGLAPIAVIGNDGSWQQIAREQVDMLGDDVGTVLSRCDYHRVAEGYGGVGLLLTEDAGIDETLDRARE
AARAGRPVCINVHLRRSEFRKGSISM

Sequences:

>Translated_586_residues
MAEATNGGAVIAAALQRAGVGHLFTLCGGHISPILVECKRRGLRVVDARDEANAVFAADAMARLTGRPGVAAVTAGPGVT
NAVTALENARLAQSPVVLLGGATATLLRGRGALQDIDQLALMRPIAKWATRVTTVGGLRPTLERALALARGGVPGPVFVE
VPVDLLYDEALVRDLYRRESGADRVSGVAGTALRLYLEAHLARQFRAPALPSLEDLPARIAERIDLRRGTSGRVERIAER
LSRAERPVLILGSQALAGCEDPERLAAAVRALGVPVYLGGMARGLLGRRDALQFRHARGRALKEADVVVVAGFPFDFRLG
YGRGFGRGAFIAAANLSAAELRKNRSPDVAVEMHPGELLVALAAKAGKPQARDAWFGALREREAARDREIAAGAEATGEL
VNPLRFLLRLEEKMAEDAALVVDGGDFVATAAYTLRPRAPLAWLDPGVFGTLGVGGGFATAAALARPGREVWLLYGDGSC
AYSLAEFDTFARHGLAPIAVIGNDGSWQQIAREQVDMLGDDVGTVLSRCDYHRVAEGYGGVGLLLTEDAGIDETLDRARE
AARAGRPVCINVHLRRSEFRKGSISM
>Mature_585_residues
AEATNGGAVIAAALQRAGVGHLFTLCGGHISPILVECKRRGLRVVDARDEANAVFAADAMARLTGRPGVAAVTAGPGVTN
AVTALENARLAQSPVVLLGGATATLLRGRGALQDIDQLALMRPIAKWATRVTTVGGLRPTLERALALARGGVPGPVFVEV
PVDLLYDEALVRDLYRRESGADRVSGVAGTALRLYLEAHLARQFRAPALPSLEDLPARIAERIDLRRGTSGRVERIAERL
SRAERPVLILGSQALAGCEDPERLAAAVRALGVPVYLGGMARGLLGRRDALQFRHARGRALKEADVVVVAGFPFDFRLGY
GRGFGRGAFIAAANLSAAELRKNRSPDVAVEMHPGELLVALAAKAGKPQARDAWFGALREREAARDREIAAGAEATGELV
NPLRFLLRLEEKMAEDAALVVDGGDFVATAAYTLRPRAPLAWLDPGVFGTLGVGGGFATAAALARPGREVWLLYGDGSCA
YSLAEFDTFARHGLAPIAVIGNDGSWQQIAREQVDMLGDDVGTVLSRCDYHRVAEGYGGVGLLLTEDAGIDETLDRAREA
ARAGRPVCINVHLRRSEFRKGSISM

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=594, Percent_Identity=47.3063973063973, Blast_Score=500, Evalue=1e-141,
Organism=Homo sapiens, GI93004078, Length=602, Percent_Identity=25.0830564784053, Blast_Score=172, Evalue=7e-43,
Organism=Escherichia coli, GI87081685, Length=604, Percent_Identity=26.6556291390728, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1790104, Length=538, Percent_Identity=24.5353159851301, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788716, Length=590, Percent_Identity=25.0847457627119, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1786717, Length=592, Percent_Identity=23.3108108108108, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17542570, Length=594, Percent_Identity=45.7912457912458, Blast_Score=492, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17531301, Length=599, Percent_Identity=26.8781302170284, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=599, Percent_Identity=26.8781302170284, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320816, Length=585, Percent_Identity=23.7606837606838, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=601, Percent_Identity=24.6256239600666, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922626, Length=594, Percent_Identity=25.9259259259259, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62103; Mature: 61972

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEATNGGAVIAAALQRAGVGHLFTLCGGHISPILVECKRRGLRVVDARDEANAVFAADA
CCCCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCEEEHHHH
MARLTGRPGVAAVTAGPGVTNAVTALENARLAQSPVVLLGGATATLLRGRGALQDIDQLA
HHHHCCCCCEEEEECCCCCHHHHHHHHHCHHCCCCEEEECCCHHHHHHCCCCHHHHHHHH
LMRPIAKWATRVTTVGGLRPTLERALALARGGVPGPVFVEVPVDLLYDEALVRDLYRRES
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCC
GADRVSGVAGTALRLYLEAHLARQFRAPALPSLEDLPARIAERIDLRRGTSGRVERIAER
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LSRAERPVLILGSQALAGCEDPERLAAAVRALGVPVYLGGMARGLLGRRDALQFRHARGR
HHHCCCCEEEECCHHHCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHHHHHCCC
ALKEADVVVVAGFPFDFRLGYGRGFGRGAFIAAANLSAAELRKNRSPDVAVEMHPGELLV
CCCCCCEEEEECCCEEEEECCCCCCCCCEEEEECCCCHHHHHCCCCCCEEEEECCCCEEE
ALAAKAGKPQARDAWFGALREREAARDREIAAGAEATGELVNPLRFLLRLEEKMAEDAAL
EEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VVDGGDFVATAAYTLRPRAPLAWLDPGVFGTLGVGGGFATAAALARPGREVWLLYGDGSC
EEECCCEEEEEEEEECCCCCEEEECCCCEEECCCCCHHHHHHHHHCCCCEEEEEEECCCC
AYSLAEFDTFARHGLAPIAVIGNDGSWQQIAREQVDMLGDDVGTVLSRCDYHRVAEGYGG
EEHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCCC
VGLLLTEDAGIDETLDRAREAARAGRPVCINVHLRRSEFRKGSISM
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHCCCCCCCC
>Mature Secondary Structure 
AEATNGGAVIAAALQRAGVGHLFTLCGGHISPILVECKRRGLRVVDARDEANAVFAADA
CCCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCEEEHHHH
MARLTGRPGVAAVTAGPGVTNAVTALENARLAQSPVVLLGGATATLLRGRGALQDIDQLA
HHHHCCCCCEEEEECCCCCHHHHHHHHHCHHCCCCEEEECCCHHHHHHCCCCHHHHHHHH
LMRPIAKWATRVTTVGGLRPTLERALALARGGVPGPVFVEVPVDLLYDEALVRDLYRRES
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCC
GADRVSGVAGTALRLYLEAHLARQFRAPALPSLEDLPARIAERIDLRRGTSGRVERIAER
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LSRAERPVLILGSQALAGCEDPERLAAAVRALGVPVYLGGMARGLLGRRDALQFRHARGR
HHHCCCCEEEECCHHHCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHHHHHCCC
ALKEADVVVVAGFPFDFRLGYGRGFGRGAFIAAANLSAAELRKNRSPDVAVEMHPGELLV
CCCCCCEEEEECCCEEEEECCCCCCCCCEEEEECCCCHHHHHCCCCCCEEEEECCCCEEE
ALAAKAGKPQARDAWFGALREREAARDREIAAGAEATGELVNPLRFLLRLEEKMAEDAAL
EEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VVDGGDFVATAAYTLRPRAPLAWLDPGVFGTLGVGGGFATAAALARPGREVWLLYGDGSC
EEECCCEEEEEEEEECCCCCEEEECCCCEEECCCCCHHHHHHHHHCCCCEEEEEEECCCC
AYSLAEFDTFARHGLAPIAVIGNDGSWQQIAREQVDMLGDDVGTVLSRCDYHRVAEGYGG
EEHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCCC
VGLLLTEDAGIDETLDRAREAARAGRPVCINVHLRRSEFRKGSISM
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]