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The map label for this gene is napA [H]

Identifier: 220916074

GI number: 220916074

Start: 1080171

End: 1082429

Strand: Reverse

Name: napA [H]

Synonym: A2cp1_0961

Alternate gene names: 220916074

Gene position: 1082429-1080171 (Counterclockwise)

Preceding gene: 220916075

Following gene: 220916073

Centisome position: 21.52

GC content: 72.24

Gene sequence:

>2259_bases
ATGCCTCTCACGCGCCGTGACTTCCTGAAGGCCTCCGCCGCCGCCTCCGCCCTGGCCGGGCTGGGGCTCCCCGTGGACGC
GCTCGCCGCCGAGGGCGGTGAGCGCTGGGTGAAGTCGGTCTGCCGCTACTGCGGCACCGGCTGCGGCCTGTACGTCGGCG
TCCGCGACGGCAAGGTGTTCGCCGTCAAGGGCGACCGGGACAACCACAACCGCGGCCTGCTCTGCCTCAAGGGGTTCCTG
CTGCCGCAGATCATGTCGGCGCCGGACCGCTGCCTGCACCCGCTGGTGCGCAAAGGCGGCAAGCTGGTGCGCGCGAGCTG
GGACGAGGCGATGTCGCTCGTCGCGCGGCGGTTCCGCGAGGCCGTCGACGCGCACGGGCCGGGCGCGGTGGGCTTCTACG
GGTCCGGCCAGGGGCTCACCGAGGAGACCTACGCGGCCAACAAGCTGTTCAAGGCGGGCCTGCGCACCAACCACGTCGAG
GGCAACCCGCGCCTGTGCATGGCCTCGGCGGTGGGCGGATACCTGAGCACCTACGGCAAGGACGAGCCGATGGGCTGCTA
CGAGGACCTCGACCACGCCGACGTCATGCTGCTGGTCGGCTCGAACGCCGCGGAGGCCCACCCGATCCTGTTCGAGCGGA
TGGCGCGCCGGAAGCAGACCGGCAAGGACGTGAAGGTGGTCGTGCTCGATCCGCGGCGCACCCCGACCGCGCGCATCGCC
GACCTGCACCTCTCGTTCACCCCGGGCACCGACCTCGCCATCCTGAACGCGATGGCGAACGTGCTCGTCGCCGAGGGGCT
GGTGGACGAGGCGTTCGTGAAGGCGCACGTCGCGTTCGGCGAGGGCAAGGACGTGAACAGGGCCTGGGAGGACTACCGGG
CCTTCCTCGCCCCGTACACCCCGGTGCGCGCCGCCGAGCTCTCCGGCGCGCGCGCCGGGGACATCGTCACCGCCGCGCGC
TGGTTCGGCGAGAAGGGACGCACCGCCACGTCCATGTGGTGCATGGGACTCAACCAGCGGACCCGCGGCGTGTGGGCGAA
CAACCTCGTCCACAACCTGCACCTGCTGACCGGCAAGATCGGCCTCCCCGGCTCGACCCCGCTGTCGCTCACCGGCCAGC
CCAATGCCTGCGGCGGCGTCCGGGACGGCGGGGCGCTCTCGCACCTGCTGCCCTACGGCCGGCTGATCGCGAACGAGAAG
CACCGCGCCGAGATGGAGAAGCTCTGGAACGTGCCGCCCGGCACGATCCAGCCCGAGAACGGCAAGCCCACCATGGACCT
GTTCCAGGCGCTGGAGCAGGGCGACCTGAAGGCGCTGTACGTGATGACCACGAACCCGGCGCAGTCGCTGCCGAACGTGG
ACCGGTACCGGAAGGCGCTCGAGGCGCGCAAGGCGTTCCTGGTGGTCACCGAGGCGTTCCACCCGACGCGCACCTCCGAG
CTCGCAGACGTCGTGCTGCCGGCGGCGCTGTGGGCCGAGAAGGAGGGCGTGTACGGCTGCACCGAGCGGCGCTACCAGCT
GCTCGAGCAGGCGGTCCGCCCCGCCGGCGAGGCCCGGCCGGACTTCGCCATCCTGTGCGACCTGGCGGCGCGGCTCGGGC
ACGGCGCGCTCCTGCCGTGGAAGGGGCCGGCGGAGGCGTGGGACGAGATCCTGACGCTGAGCAAGGGCACCGCGTACGAC
TTCACCGGCATGACCCGGGCCCGCCTGGCCGCGTCGCACGGGCTGCTCTGGCCGCTGCCGGCCGAGGGCCATCCGGGCAC
GAAGCGCCGCTTCGTGAAGGGCGAGGACCCGCTCGTCCCGGCCGACGCGCCCGACCGGATCCGCTTCTACGGCCGGCCCG
ACGCCCGCGCGGTGATCTGGCTCCGGCCGCAGCAGCCGCCCGCCGAGGTGACCGACGCCGCGTACCCGCTGGTCCTCACC
ACCGGCCGCGTCATCGAGCACTGGCACACCGGGACCATGACCCGGAACTGCAAGGAGCTGCGCCACGCGAACGCCGAGTC
GGTGGTGGAGCTGCACCCGGACGACGGCAAGCCGCTCGGGATCCGGACCGGCGACGAGGTGCGCGTCAGCTCGCGCCGCG
GCGCGGAGACGTTCAAGGCGAAGCTGGTGGACGGGGCGCGCCCGGGCACGGTGTTCGTGCACATGCACGACCCGCAGCGC
ATGTGCAACCGGCTCACCATCGACGCGGTGGACCCGGTCTCGAAGCAGCCCGAGTTCAAGATCTGCGCGGTGAAGATCGA
GCCGATCCGCAAGGCCTGA

Upstream 100 bases:

>100_bases
CGGGCCCGGCGGCTGCTTCACCTGCCACGAGCCGCACGCCCTCACCGCGACGGGCGAGCCCCCGCATTCCAAGTGACCCG
CGCAGCCCGAGGAGAACATC

Downstream 100 bases:

>100_bases
GCCGGACGGAGGTCGACATGCTGGTCGCTGGGGTGGTGATCGAGACCGTGCCGGGGGCGGCACCCCGGGTCGCCGCGCGG
CTGCTCTCCGAGCCCGCGCT

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MPLTRRDFLKASAAASALAGLGLPVDALAAEGGERWVKSVCRYCGTGCGLYVGVRDGKVFAVKGDRDNHNRGLLCLKGFL
LPQIMSAPDRCLHPLVRKGGKLVRASWDEAMSLVARRFREAVDAHGPGAVGFYGSGQGLTEETYAANKLFKAGLRTNHVE
GNPRLCMASAVGGYLSTYGKDEPMGCYEDLDHADVMLLVGSNAAEAHPILFERMARRKQTGKDVKVVVLDPRRTPTARIA
DLHLSFTPGTDLAILNAMANVLVAEGLVDEAFVKAHVAFGEGKDVNRAWEDYRAFLAPYTPVRAAELSGARAGDIVTAAR
WFGEKGRTATSMWCMGLNQRTRGVWANNLVHNLHLLTGKIGLPGSTPLSLTGQPNACGGVRDGGALSHLLPYGRLIANEK
HRAEMEKLWNVPPGTIQPENGKPTMDLFQALEQGDLKALYVMTTNPAQSLPNVDRYRKALEARKAFLVVTEAFHPTRTSE
LADVVLPAALWAEKEGVYGCTERRYQLLEQAVRPAGEARPDFAILCDLAARLGHGALLPWKGPAEAWDEILTLSKGTAYD
FTGMTRARLAASHGLLWPLPAEGHPGTKRRFVKGEDPLVPADAPDRIRFYGRPDARAVIWLRPQQPPAEVTDAAYPLVLT
TGRVIEHWHTGTMTRNCKELRHANAESVVELHPDDGKPLGIRTGDEVRVSSRRGAETFKAKLVDGARPGTVFVHMHDPQR
MCNRLTIDAVDPVSKQPEFKICAVKIEPIRKA

Sequences:

>Translated_752_residues
MPLTRRDFLKASAAASALAGLGLPVDALAAEGGERWVKSVCRYCGTGCGLYVGVRDGKVFAVKGDRDNHNRGLLCLKGFL
LPQIMSAPDRCLHPLVRKGGKLVRASWDEAMSLVARRFREAVDAHGPGAVGFYGSGQGLTEETYAANKLFKAGLRTNHVE
GNPRLCMASAVGGYLSTYGKDEPMGCYEDLDHADVMLLVGSNAAEAHPILFERMARRKQTGKDVKVVVLDPRRTPTARIA
DLHLSFTPGTDLAILNAMANVLVAEGLVDEAFVKAHVAFGEGKDVNRAWEDYRAFLAPYTPVRAAELSGARAGDIVTAAR
WFGEKGRTATSMWCMGLNQRTRGVWANNLVHNLHLLTGKIGLPGSTPLSLTGQPNACGGVRDGGALSHLLPYGRLIANEK
HRAEMEKLWNVPPGTIQPENGKPTMDLFQALEQGDLKALYVMTTNPAQSLPNVDRYRKALEARKAFLVVTEAFHPTRTSE
LADVVLPAALWAEKEGVYGCTERRYQLLEQAVRPAGEARPDFAILCDLAARLGHGALLPWKGPAEAWDEILTLSKGTAYD
FTGMTRARLAASHGLLWPLPAEGHPGTKRRFVKGEDPLVPADAPDRIRFYGRPDARAVIWLRPQQPPAEVTDAAYPLVLT
TGRVIEHWHTGTMTRNCKELRHANAESVVELHPDDGKPLGIRTGDEVRVSSRRGAETFKAKLVDGARPGTVFVHMHDPQR
MCNRLTIDAVDPVSKQPEFKICAVKIEPIRKA
>Mature_751_residues
PLTRRDFLKASAAASALAGLGLPVDALAAEGGERWVKSVCRYCGTGCGLYVGVRDGKVFAVKGDRDNHNRGLLCLKGFLL
PQIMSAPDRCLHPLVRKGGKLVRASWDEAMSLVARRFREAVDAHGPGAVGFYGSGQGLTEETYAANKLFKAGLRTNHVEG
NPRLCMASAVGGYLSTYGKDEPMGCYEDLDHADVMLLVGSNAAEAHPILFERMARRKQTGKDVKVVVLDPRRTPTARIAD
LHLSFTPGTDLAILNAMANVLVAEGLVDEAFVKAHVAFGEGKDVNRAWEDYRAFLAPYTPVRAAELSGARAGDIVTAARW
FGEKGRTATSMWCMGLNQRTRGVWANNLVHNLHLLTGKIGLPGSTPLSLTGQPNACGGVRDGGALSHLLPYGRLIANEKH
RAEMEKLWNVPPGTIQPENGKPTMDLFQALEQGDLKALYVMTTNPAQSLPNVDRYRKALEARKAFLVVTEAFHPTRTSEL
ADVVLPAALWAEKEGVYGCTERRYQLLEQAVRPAGEARPDFAILCDLAARLGHGALLPWKGPAEAWDEILTLSKGTAYDF
TGMTRARLAASHGLLWPLPAEGHPGTKRRFVKGEDPLVPADAPDRIRFYGRPDARAVIWLRPQQPPAEVTDAAYPLVLTT
GRVIEHWHTGTMTRNCKELRHANAESVVELHPDDGKPLGIRTGDEVRVSSRRGAETFKAKLVDGARPGTVFVHMHDPQRM
CNRLTIDAVDPVSKQPEFKICAVKIEPIRKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=833, Percent_Identity=39.015606242497, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI3868721, Length=723, Percent_Identity=30.4287690179806, Blast_Score=342, Evalue=7e-95,
Organism=Escherichia coli, GI3868719, Length=663, Percent_Identity=24.132730015083, Blast_Score=143, Evalue=5e-35,
Organism=Escherichia coli, GI87081797, Length=796, Percent_Identity=23.7437185929648, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI3868720, Length=665, Percent_Identity=23.4586466165414, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI171474008, Length=790, Percent_Identity=24.5569620253165, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693196, Length=711, Percent_Identity=23.6286919831224, Blast_Score=104, Evalue=3e-23,
Organism=Escherichia coli, GI1787870, Length=407, Percent_Identity=28.009828009828, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1787231, Length=720, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787778, Length=495, Percent_Identity=22.8282828282828, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 82032; Mature: 81901

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTRRDFLKASAAASALAGLGLPVDALAAEGGERWVKSVCRYCGTGCGLYVGVRDGKVF
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEE
AVKGDRDNHNRGLLCLKGFLLPQIMSAPDRCLHPLVRKGGKLVRASWDEAMSLVARRFRE
EEECCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHH
AVDAHGPGAVGFYGSGQGLTEETYAANKLFKAGLRTNHVEGNPRLCMASAVGGYLSTYGK
HHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHCCC
DEPMGCYEDLDHADVMLLVGSNAAEAHPILFERMARRKQTGKDVKVVVLDPRRTPTARIA
CCCCCHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEE
DLHLSFTPGTDLAILNAMANVLVAEGLVDEAFVKAHVAFGEGKDVNRAWEDYRAFLAPYT
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCC
PVRAAELSGARAGDIVTAARWFGEKGRTATSMWCMGLNQRTRGVWANNLVHNLHLLTGKI
CCHHHHCCCCCCCCHHHHHHHHCCCCCEEHHHHEECCCCCCCCHHHHHHHHHEEEEEECC
GLPGSTPLSLTGQPNACGGVRDGGALSHLLPYGRLIANEKHRAEMEKLWNVPPGTIQPEN
CCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
GKPTMDLFQALEQGDLKALYVMTTNPAQSLPNVDRYRKALEARKAFLVVTEAFHPTRTSE
CCCHHHHHHHHHCCCCEEEEEEECCHHHHCCCHHHHHHHHHHHHEEEEEEECCCCCCHHH
LADVVLPAALWAEKEGVYGCTERRYQLLEQAVRPAGEARPDFAILCDLAARLGHGALLPW
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCC
KGPAEAWDEILTLSKGTAYDFTGMTRARLAASHGLLWPLPAEGHPGTKRRFVKGEDPLVP
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCCEECCCCCCCC
ADAPDRIRFYGRPDARAVIWLRPQQPPAEVTDAAYPLVLTTGRVIEHWHTGTMTRNCKEL
CCCCCCEEEECCCCCCEEEEECCCCCCCHHHCCCCCEEEECCHHHHHHCCCCHHHHHHHH
RHANAESVVELHPDDGKPLGIRTGDEVRVSSRRGAETFKAKLVDGARPGTVFVHMHDPQR
HHCCCCCEEEECCCCCCEECCCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEEECCHHH
MCNRLTIDAVDPVSKQPEFKICAVKIEPIRKA
HHCEEEEECCCCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
PLTRRDFLKASAAASALAGLGLPVDALAAEGGERWVKSVCRYCGTGCGLYVGVRDGKVF
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEE
AVKGDRDNHNRGLLCLKGFLLPQIMSAPDRCLHPLVRKGGKLVRASWDEAMSLVARRFRE
EEECCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHH
AVDAHGPGAVGFYGSGQGLTEETYAANKLFKAGLRTNHVEGNPRLCMASAVGGYLSTYGK
HHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHCCC
DEPMGCYEDLDHADVMLLVGSNAAEAHPILFERMARRKQTGKDVKVVVLDPRRTPTARIA
CCCCCHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEE
DLHLSFTPGTDLAILNAMANVLVAEGLVDEAFVKAHVAFGEGKDVNRAWEDYRAFLAPYT
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCC
PVRAAELSGARAGDIVTAARWFGEKGRTATSMWCMGLNQRTRGVWANNLVHNLHLLTGKI
CCHHHHCCCCCCCCHHHHHHHHCCCCCEEHHHHEECCCCCCCCHHHHHHHHHEEEEEECC
GLPGSTPLSLTGQPNACGGVRDGGALSHLLPYGRLIANEKHRAEMEKLWNVPPGTIQPEN
CCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
GKPTMDLFQALEQGDLKALYVMTTNPAQSLPNVDRYRKALEARKAFLVVTEAFHPTRTSE
CCCHHHHHHHHHCCCCEEEEEEECCHHHHCCCHHHHHHHHHHHHEEEEEEECCCCCCHHH
LADVVLPAALWAEKEGVYGCTERRYQLLEQAVRPAGEARPDFAILCDLAARLGHGALLPW
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCC
KGPAEAWDEILTLSKGTAYDFTGMTRARLAASHGLLWPLPAEGHPGTKRRFVKGEDPLVP
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCCEECCCCCCCC
ADAPDRIRFYGRPDARAVIWLRPQQPPAEVTDAAYPLVLTTGRVIEHWHTGTMTRNCKEL
CCCCCCEEEECCCCCCEEEEECCCCCCCHHHCCCCCEEEECCHHHHHHCCCCHHHHHHHH
RHANAESVVELHPDDGKPLGIRTGDEVRVSSRRGAETFKAKLVDGARPGTVFVHMHDPQR
HHCCCCCEEEECCCCCCEECCCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEEECCHHH
MCNRLTIDAVDPVSKQPEFKICAVKIEPIRKA
HHCEEEEECCCCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368307; 9367852 [H]