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The map label for this gene is ligA

Identifier: 220915846

GI number: 220915846

Start: 819961

End: 822024

Strand: Direct

Name: ligA

Synonym: A2cp1_0729

Alternate gene names: 220915846

Gene position: 819961-822024 (Clockwise)

Preceding gene: 220915845

Following gene: 220915847

Centisome position: 16.3

GC content: 73.84

Gene sequence:

>2064_bases
ATGAAGAAGGCCGACGCTTCGGCGCGTGCCCGCGAGCTGCGCGACCGGATCCGCGCGGCGGACCACGCCTACTACGTCCT
CGACCAGCCGCTCCTCGCCGACGCCGAGTACGACCGGCTCATGCACGAGCTGCAGGCGCTCGAGGCCGAGCACCCGGAGC
TCGTCACCGCGGACTCGCCCACGCAGCGGGTCTCCGGCGCTCCCTCGGAGCGCTTCGAGCGGGTGGTGCACCGCGAGCCC
ATGCTCTCGCTCGGCAACGTGCAGAGCGACGACGAGCTCCACGAGTTCGACGCGCGGGTGCGCCGCCTGCTCGGGCTGCC
CGACGGAGAGCCGGTCGGCTACGTGGTCGAGCCGAAGCTCGACGGGCTCGCGGTGGAGCTGGTGTACCGCGACGGTGCGT
TCACGAGCGGTTCCACGCGAGGCGACGGCGTGAACGGCGAGGACGTCACCGCCAACCTGCGCGTGGTGGGCGGGCTGGGC
GCGAACCGCGGCGTGCCGCACGCGCTCGAGGGGAGGCCGCCGCCGCGGGTCGAGGTCCGCGGCGAGGTGCTGCTGTTCAA
GGAGCACTTCGAGGCGATGAACCGGCAGCTGGTCCGCGCCGGCGAGGAGCCGTTCGCGAACCCGCGCAACGCCGCCGCCG
GGACGCTGCGCCAGCTCGACTGGCGCGTCACCGCCCGCCGCCCGCTCTCCTTCATCGCCTACGAGGCGCTGCTGCCGGGC
GGCGATCCGTGGCGCACGCACTGGGAGAAGCTGGAGGAGCTCGCCGCCTGGGGCTTCGAGACGAACGCGGAGAACCGCCG
CTGCCGCGGCCTCGCCGAGGTGCTCGCCTACCGCGATCGCATGGCCGAGCGCCGCTTCGAGCTCCCCTACGACACCGACG
GCATCGTGGTGAAGGTGGACGACCTCGACTGGCGGCGGCGGCTCGGCGCGGCCTCGAAGTTCCCGCGGTGGGCCGTCGCC
TTCAAGTACCCGCCGCAGGAGGAGGCGACGAGGATCCGCAGGATCTGGGCCTCGGTGGGCCGCACCGGCGTGCTCACGCC
GGTGGTGGACTTCGACCCCGTCCGCCTCTCCGGCGCGATGGTCGCGCGCGCCACGCTGCACAACGAGGACGAGATGCGCC
GCAAGGACATCCTCGAGGGCGACTGGGTGCTGGTGCGGCGCGCCGGCGAGGTGATCCCGGAGGTGGTGAAGCCGCTCCCC
GAGCGCCGGACCGGCGCGGAGCAGCCGTTCCGGTTCCCGGCCGAGTGCCCGGTGTGCGGCGCGCGCGTGGTCCGCGAGGA
GGGCGAGAAGGTCTACCGCTGCACGGGCGCCGCCTGCCCGGCGCAGCTCGTCGGTCGGCTCTGCCACTTCGCGCAGCGCC
GTGCGCTCGACATCGAGGGGCTGGGCGAGAAGCTCGCCGCCGGCCTGGTGGAGCGCGGGCAGGTGAAGGACTTCGCCGAC
CTGTACGCGGTGCCGTTCGAGGTGTGGCAGCAGCTGTTCAGCCGCCCGCGCAAGGAGCAGGACGCGGGGGCGGCGCGCGA
GCTGCCGGAGAAGAGCGCGCAGAACATGGTCGCGGCGCTGGAGCGGTCGCGCAAGACGACGCTCAGGCGCTTCCTGTTCG
CGCTCGGGATCCCGCAGGTGGGCGAGGCGACCGCGGCCACGCTGGCGCGCCACTTCGGCGACCTCGCGCGCGTCATGGAC
GCGGACGAGGAGGCGCTGAAGGGCGTCCGCGACGTGGGCCCGGAGACCGCCGCGGAGATCCGCGCCTGGACGCAGGAGCC
GCAGAACCGGCGGGTGGTCGAGCGGCTCCTCGCCGCCGGGGTGACGCCCGAGGCCGAGGTGGTGGAGGCGCGCGGCCCGT
TTGCCGGCAAGACGGTGGTGCTCACCGGCGGGCTCTCGACGATGTCGCGCGACGACGCCAAGGCGGAGATCGAGCGGCGC
GGGGGCAAGGTGTCGGGCAGCGTCTCGCGCAAGACCTACCTGGTGGTGGCCGGGGAGGACGCCGGCTCCAAGCTGGAGAA
GGCGCGATCGCTGGGCGTTCGGATCGCCGGCGAGGAGGAGTTCGTGCGCCTGCTGAAGGAGTGA

Upstream 100 bases:

>100_bases
CTTCTGATCCGTCCTCGCACCTCCGCCTCGCTCCTGGCGCCCACGCCGGGGGCCGCATTCCTCGTTGCCGGCGGCCCGCC
GCCGCGCCATCCTTCGCGCC

Downstream 100 bases:

>100_bases
GGGCGATGGCCGATCGGGTCCGTGCGCGCATCGTGGTGTCCGGCCGCGTCCAGGGCGTGGCCTTCCGCCAGTCCACGGCG
GACGAGGCGCGTCGCCTGGG

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MKKADASARARELRDRIRAADHAYYVLDQPLLADAEYDRLMHELQALEAEHPELVTADSPTQRVSGAPSERFERVVHREP
MLSLGNVQSDDELHEFDARVRRLLGLPDGEPVGYVVEPKLDGLAVELVYRDGAFTSGSTRGDGVNGEDVTANLRVVGGLG
ANRGVPHALEGRPPPRVEVRGEVLLFKEHFEAMNRQLVRAGEEPFANPRNAAAGTLRQLDWRVTARRPLSFIAYEALLPG
GDPWRTHWEKLEELAAWGFETNAENRRCRGLAEVLAYRDRMAERRFELPYDTDGIVVKVDDLDWRRRLGAASKFPRWAVA
FKYPPQEEATRIRRIWASVGRTGVLTPVVDFDPVRLSGAMVARATLHNEDEMRRKDILEGDWVLVRRAGEVIPEVVKPLP
ERRTGAEQPFRFPAECPVCGARVVREEGEKVYRCTGAACPAQLVGRLCHFAQRRALDIEGLGEKLAAGLVERGQVKDFAD
LYAVPFEVWQQLFSRPRKEQDAGAARELPEKSAQNMVAALERSRKTTLRRFLFALGIPQVGEATAATLARHFGDLARVMD
ADEEALKGVRDVGPETAAEIRAWTQEPQNRRVVERLLAAGVTPEAEVVEARGPFAGKTVVLTGGLSTMSRDDAKAEIERR
GGKVSGSVSRKTYLVVAGEDAGSKLEKARSLGVRIAGEEEFVRLLKE

Sequences:

>Translated_687_residues
MKKADASARARELRDRIRAADHAYYVLDQPLLADAEYDRLMHELQALEAEHPELVTADSPTQRVSGAPSERFERVVHREP
MLSLGNVQSDDELHEFDARVRRLLGLPDGEPVGYVVEPKLDGLAVELVYRDGAFTSGSTRGDGVNGEDVTANLRVVGGLG
ANRGVPHALEGRPPPRVEVRGEVLLFKEHFEAMNRQLVRAGEEPFANPRNAAAGTLRQLDWRVTARRPLSFIAYEALLPG
GDPWRTHWEKLEELAAWGFETNAENRRCRGLAEVLAYRDRMAERRFELPYDTDGIVVKVDDLDWRRRLGAASKFPRWAVA
FKYPPQEEATRIRRIWASVGRTGVLTPVVDFDPVRLSGAMVARATLHNEDEMRRKDILEGDWVLVRRAGEVIPEVVKPLP
ERRTGAEQPFRFPAECPVCGARVVREEGEKVYRCTGAACPAQLVGRLCHFAQRRALDIEGLGEKLAAGLVERGQVKDFAD
LYAVPFEVWQQLFSRPRKEQDAGAARELPEKSAQNMVAALERSRKTTLRRFLFALGIPQVGEATAATLARHFGDLARVMD
ADEEALKGVRDVGPETAAEIRAWTQEPQNRRVVERLLAAGVTPEAEVVEARGPFAGKTVVLTGGLSTMSRDDAKAEIERR
GGKVSGSVSRKTYLVVAGEDAGSKLEKARSLGVRIAGEEEFVRLLKE
>Mature_687_residues
MKKADASARARELRDRIRAADHAYYVLDQPLLADAEYDRLMHELQALEAEHPELVTADSPTQRVSGAPSERFERVVHREP
MLSLGNVQSDDELHEFDARVRRLLGLPDGEPVGYVVEPKLDGLAVELVYRDGAFTSGSTRGDGVNGEDVTANLRVVGGLG
ANRGVPHALEGRPPPRVEVRGEVLLFKEHFEAMNRQLVRAGEEPFANPRNAAAGTLRQLDWRVTARRPLSFIAYEALLPG
GDPWRTHWEKLEELAAWGFETNAENRRCRGLAEVLAYRDRMAERRFELPYDTDGIVVKVDDLDWRRRLGAASKFPRWAVA
FKYPPQEEATRIRRIWASVGRTGVLTPVVDFDPVRLSGAMVARATLHNEDEMRRKDILEGDWVLVRRAGEVIPEVVKPLP
ERRTGAEQPFRFPAECPVCGARVVREEGEKVYRCTGAACPAQLVGRLCHFAQRRALDIEGLGEKLAAGLVERGQVKDFAD
LYAVPFEVWQQLFSRPRKEQDAGAARELPEKSAQNMVAALERSRKTTLRRFLFALGIPQVGEATAATLARHFGDLARVMD
ADEEALKGVRDVGPETAAEIRAWTQEPQNRRVVERLLAAGVTPEAEVVEARGPFAGKTVVLTGGLSTMSRDDAKAEIERR
GGKVSGSVSRKTYLVVAGEDAGSKLEKARSLGVRIAGEEEFVRLLKE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=681, Percent_Identity=47.2834067547724, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI87082305, Length=491, Percent_Identity=26.6802443991853, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ANAD2 (B8JD56)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491150.1
- ProteinModelPortal:   B8JD56
- GeneID:   7299114
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0729
- HOGENOM:   HBG620317
- ProtClustDB:   CLSK945187
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76422; Mature: 76422

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 119-119 BINDING 117-117 BINDING 140-140 BINDING 182-182 BINDING 298-298 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKADASARARELRDRIRAADHAYYVLDQPLLADAEYDRLMHELQALEAEHPELVTADSP
CCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHCCCHHHHHHHHHHHHHCCCCCEEECCCC
TQRVSGAPSERFERVVHREPMLSLGNVQSDDELHEFDARVRRLLGLPDGEPVGYVVEPKL
HHHHCCCCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
DGLAVELVYRDGAFTSGSTRGDGVNGEDVTANLRVVGGLGANRGVPHALEGRPPPRVEVR
CCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEC
GEVLLFKEHFEAMNRQLVRAGEEPFANPRNAAAGTLRQLDWRVTARRPLSFIAYEALLPG
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHCCC
GDPWRTHWEKLEELAAWGFETNAENRRCRGLAEVLAYRDRMAERRFELPYDTDGIVVKVD
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
DLDWRRRLGAASKFPRWAVAFKYPPQEEATRIRRIWASVGRTGVLTPVVDFDPVRLSGAM
CCHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHH
VARATLHNEDEMRRKDILEGDWVLVRRAGEVIPEVVKPLPERRTGAEQPFRFPAECPVCG
HHHHHHCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
ARVVREEGEKVYRCTGAACPAQLVGRLCHFAQRRALDIEGLGEKLAAGLVERGQVKDFAD
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH
LYAVPFEVWQQLFSRPRKEQDAGAARELPEKSAQNMVAALERSRKTTLRRFLFALGIPQV
HHHHHHHHHHHHHHCCCCCHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GEATAATLARHFGDLARVMDADEEALKGVRDVGPETAAEIRAWTQEPQNRRVVERLLAAG
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCC
VTPEAEVVEARGPFAGKTVVLTGGLSTMSRDDAKAEIERRGGKVSGSVSRKTYLVVAGED
CCCCHHHEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCC
AGSKLEKARSLGVRIAGEEEFVRLLKE
CCHHHHHHHHCCCEEECHHHHHHHHCC
>Mature Secondary Structure
MKKADASARARELRDRIRAADHAYYVLDQPLLADAEYDRLMHELQALEAEHPELVTADSP
CCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHCCCHHHHHHHHHHHHHCCCCCEEECCCC
TQRVSGAPSERFERVVHREPMLSLGNVQSDDELHEFDARVRRLLGLPDGEPVGYVVEPKL
HHHHCCCCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
DGLAVELVYRDGAFTSGSTRGDGVNGEDVTANLRVVGGLGANRGVPHALEGRPPPRVEVR
CCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEC
GEVLLFKEHFEAMNRQLVRAGEEPFANPRNAAAGTLRQLDWRVTARRPLSFIAYEALLPG
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHCCC
GDPWRTHWEKLEELAAWGFETNAENRRCRGLAEVLAYRDRMAERRFELPYDTDGIVVKVD
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
DLDWRRRLGAASKFPRWAVAFKYPPQEEATRIRRIWASVGRTGVLTPVVDFDPVRLSGAM
CCHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHH
VARATLHNEDEMRRKDILEGDWVLVRRAGEVIPEVVKPLPERRTGAEQPFRFPAECPVCG
HHHHHHCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
ARVVREEGEKVYRCTGAACPAQLVGRLCHFAQRRALDIEGLGEKLAAGLVERGQVKDFAD
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHH
LYAVPFEVWQQLFSRPRKEQDAGAARELPEKSAQNMVAALERSRKTTLRRFLFALGIPQV
HHHHHHHHHHHHHHCCCCCHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GEATAATLARHFGDLARVMDADEEALKGVRDVGPETAAEIRAWTQEPQNRRVVERLLAAG
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCC
VTPEAEVVEARGPFAGKTVVLTGGLSTMSRDDAKAEIERRGGKVSGSVSRKTYLVVAGED
CCCCHHHEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCC
AGSKLEKARSLGVRIAGEEEFVRLLKE
CCHHHHHHHHCCCEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA