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The map label for this gene is fadD25 [H]

Identifier: 220915537

GI number: 220915537

Start: 472303

End: 474063

Strand: Direct

Name: fadD25 [H]

Synonym: A2cp1_0418

Alternate gene names: 220915537

Gene position: 472303-474063 (Clockwise)

Preceding gene: 220915536

Following gene: 220915538

Centisome position: 9.39

GC content: 79.27

Gene sequence:

>1761_bases
GTGAGGCTGCGCGGGCCGCCCCAGCCGCCGCCGCGCCACACCACGCTGGTGCACGCGCTGGCGGCCGCGGCCGCGCACCC
CTCCGGCGCGACCTTCCTCGACCTGCACGAGCGCGAGACGTCCCTGCCCTGGTCGGAGGTCCGCGCCCGGGCCGAGCGCG
CCGCCGCCAACCTCGTCCACCTCGGCATCCGGCGCGGCGATCGGGTCGCCATCGTGCTCCGGACCGAGCCGGCCTTCCTG
GACGCGTTCTTCGGCGCCTGGCTGGCCGGCGCGGTGCCGGTGCCGCTCTACCCGCCGGTGCGGCTGGGCCGGCTCGACGC
GTACGCGGTGGACACCGGCCGGATGATCCAGGTGGCCGGGGCGCGCCTGGTCGTCTCCGCGGGCGGCACGCGGCGGCTGC
TCGGCGGCGCCGTCGAGCGGGGCGGGCCGGAGCTCGGGTGCGTGGACGTGTCGGCGCTCTCGGCGCTGCCCGCCCGCATC
GCGCGCGAGCCGGAGCCGGACGCGCTCGGCCTGGTGCAGTTCTCCTCCGGGACGACGGTGGACCCGAAGCCGGTCGCGCT
CACCCACCGCGCGCTCGCGGCCATGACCGACGCGCTGGTCGCGGCGACCTCGGCGGGCCCGGACGACGTGCTCGTGTCGT
GGCTGCCGCTCTACCACGACATGGGCCTCATCGGCTGCCTGCTCGCCGCGATGAGCTACCCGGGTCCGCTCGTGCTCATC
CCGCCCGAGCACTTCCTCACCCGCCCCGCGCTCTGGCTGCGCGCGCTGGCCCGGCGACGCGGCACCATCTCGGCCGCGCC
CAGCTTCGCGTACGCGTACGCGGCCGACCGGGTGCAGGACGCCGACCTCGCCGGCCTCTCGCTCGCGCCGGTGCGGCTGC
TGCTCGACGGCGCCGAGCCGGTCTCGGCGGTGGCGCTGCGCCGGTTCGCCGAGCGCTTCGCGCCGCACGGGCTCGACCCG
CGGGCGCTCGTGCCCGTGTACGGCCTCAGCGAGGCGGCGCTGGCGGTGACGTTCGCGCGGCCCGGCGGACCGCTCGGCGG
GCTCGGCGTCGACGCGGCCCGGCTCGCGTCGGAGGGCGTGGTCGCGCCCGGCGGGCGGGAGGTGATGCCGGTCGGGACCC
CGGTCCCGGGCGTCGAGGTCGAGCTGCGCACCGAGGCCGGGGCGCCGGTGGGGGAGGGGCGGATCGGGCGCGTGTTCGTG
CGCGGCCCGGCGCTCATGCGCGAGTACCTGGGCGACCCCGAGGCGACCGCGCGCGCGCTCCGGGGTGGATGGCTCGACAC
CGGCGACCTCGGCTTCGTGGTGGACGGCGCGCTCCACCTCCACGGCCGCGCCCGGGACCTCGTGATCGTGCGCGGCGCGA
ACCACGCGCCGGAGGAGTTCGAGGCGCCGCTCGCGGCGCTCCCCGGCCTGCGCCCGGGCTGCGCGGTGGCGCTCGGCTTC
GAGCCGGAGGGCGGCGCCGGCGAGGCGCTGCTCGTGCTCGCGGAGCGCGCGCGAGACGAGGAGACCCCGGATGGCGAGCT
GGAGGCGCGGGTCCGGCGCGCGGTGCTGGCGGCGACCGGCATCGCGCCGCACACCGTGCGGCTGCTCGCGCCCGGCACGC
TCCCGCGGACGTCCTCGGGCAAGCTGCGGCGCGCCGAGGCGCTGCGCCGGTTCCAGGCGGGGACGCTCACGCCGCCGCAG
CCGGTGAGCGCGATCCGCCTCGCCCTCGCCGCCGCCCGCTCGCAGCTCGCCTTCGGGCGGTCGCGTCGCCGCGGGGGCTG
A

Upstream 100 bases:

>100_bases
GCTTCCGGATCGCGCTCGACGACGAGGCCGGCCTGGCGGTCCGGACGCTCGGCGACCTCGCGCGGCTGGTGGTGGCGCGC
GCCGCGCCGGAGCTGCTGCC

Downstream 100 bases:

>100_bases
GCGGTGGTGCGGCTCCGCGACGCCGTGGTGGTGGGCGGCGGACCGGCGGGGCTCGCCTTCGCCGCCGCCGCGGCCGCGCG
CGGGCTCGACGTGCTCGTGC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MRLRGPPQPPPRHTTLVHALAAAAAHPSGATFLDLHERETSLPWSEVRARAERAAANLVHLGIRRGDRVAIVLRTEPAFL
DAFFGAWLAGAVPVPLYPPVRLGRLDAYAVDTGRMIQVAGARLVVSAGGTRRLLGGAVERGGPELGCVDVSALSALPARI
AREPEPDALGLVQFSSGTTVDPKPVALTHRALAAMTDALVAATSAGPDDVLVSWLPLYHDMGLIGCLLAAMSYPGPLVLI
PPEHFLTRPALWLRALARRRGTISAAPSFAYAYAADRVQDADLAGLSLAPVRLLLDGAEPVSAVALRRFAERFAPHGLDP
RALVPVYGLSEAALAVTFARPGGPLGGLGVDAARLASEGVVAPGGREVMPVGTPVPGVEVELRTEAGAPVGEGRIGRVFV
RGPALMREYLGDPEATARALRGGWLDTGDLGFVVDGALHLHGRARDLVIVRGANHAPEEFEAPLAALPGLRPGCAVALGF
EPEGGAGEALLVLAERARDEETPDGELEARVRRAVLAATGIAPHTVRLLAPGTLPRTSSGKLRRAEALRRFQAGTLTPPQ
PVSAIRLALAAARSQLAFGRSRRRGG

Sequences:

>Translated_586_residues
MRLRGPPQPPPRHTTLVHALAAAAAHPSGATFLDLHERETSLPWSEVRARAERAAANLVHLGIRRGDRVAIVLRTEPAFL
DAFFGAWLAGAVPVPLYPPVRLGRLDAYAVDTGRMIQVAGARLVVSAGGTRRLLGGAVERGGPELGCVDVSALSALPARI
AREPEPDALGLVQFSSGTTVDPKPVALTHRALAAMTDALVAATSAGPDDVLVSWLPLYHDMGLIGCLLAAMSYPGPLVLI
PPEHFLTRPALWLRALARRRGTISAAPSFAYAYAADRVQDADLAGLSLAPVRLLLDGAEPVSAVALRRFAERFAPHGLDP
RALVPVYGLSEAALAVTFARPGGPLGGLGVDAARLASEGVVAPGGREVMPVGTPVPGVEVELRTEAGAPVGEGRIGRVFV
RGPALMREYLGDPEATARALRGGWLDTGDLGFVVDGALHLHGRARDLVIVRGANHAPEEFEAPLAALPGLRPGCAVALGF
EPEGGAGEALLVLAERARDEETPDGELEARVRRAVLAATGIAPHTVRLLAPGTLPRTSSGKLRRAEALRRFQAGTLTPPQ
PVSAIRLALAAARSQLAFGRSRRRGG
>Mature_586_residues
MRLRGPPQPPPRHTTLVHALAAAAAHPSGATFLDLHERETSLPWSEVRARAERAAANLVHLGIRRGDRVAIVLRTEPAFL
DAFFGAWLAGAVPVPLYPPVRLGRLDAYAVDTGRMIQVAGARLVVSAGGTRRLLGGAVERGGPELGCVDVSALSALPARI
AREPEPDALGLVQFSSGTTVDPKPVALTHRALAAMTDALVAATSAGPDDVLVSWLPLYHDMGLIGCLLAAMSYPGPLVLI
PPEHFLTRPALWLRALARRRGTISAAPSFAYAYAADRVQDADLAGLSLAPVRLLLDGAEPVSAVALRRFAERFAPHGLDP
RALVPVYGLSEAALAVTFARPGGPLGGLGVDAARLASEGVVAPGGREVMPVGTPVPGVEVELRTEAGAPVGEGRIGRVFV
RGPALMREYLGDPEATARALRGGWLDTGDLGFVVDGALHLHGRARDLVIVRGANHAPEEFEAPLAALPGLRPGCAVALGF
EPEGGAGEALLVLAERARDEETPDGELEARVRRAVLAATGIAPHTVRLLAPGTLPRTSSGKLRRAEALRRFQAGTLTPPQ
PVSAIRLALAAARSQLAFGRSRRRGG

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=563, Percent_Identity=23.9786856127886, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI187761343, Length=563, Percent_Identity=23.9786856127886, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI44888818, Length=504, Percent_Identity=25.9920634920635, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI225735629, Length=502, Percent_Identity=26.4940239043825, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI45827692, Length=502, Percent_Identity=26.4940239043825, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI55749758, Length=607, Percent_Identity=24.7116968698517, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI225735625, Length=552, Percent_Identity=23.9130434782609, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI45827698, Length=386, Percent_Identity=24.6113989637306, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI45827694, Length=386, Percent_Identity=24.6113989637306, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI45827696, Length=386, Percent_Identity=24.6113989637306, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI225735627, Length=386, Percent_Identity=24.6113989637306, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788107, Length=580, Percent_Identity=26.0344827586207, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=24.8565965583174, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI145693145, Length=551, Percent_Identity=26.1343012704174, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=425, Percent_Identity=27.7647058823529, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=503, Percent_Identity=23.8568588469185, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI193202679, Length=621, Percent_Identity=23.1884057971014, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=533, Percent_Identity=24.015009380863, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18859661, Length=519, Percent_Identity=24.8554913294798, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21355181, Length=477, Percent_Identity=22.8511530398323, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922652, Length=531, Percent_Identity=25.4237288135593, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20130357, Length=565, Percent_Identity=24.4247787610619, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648260, Length=194, Percent_Identity=32.4742268041237, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24653035, Length=139, Percent_Identity=30.2158273381295, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356947, Length=141, Percent_Identity=29.7872340425532, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24654656, Length=598, Percent_Identity=22.2408026755853, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 61290; Mature: 61290

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRGPPQPPPRHTTLVHALAAAAAHPSGATFLDLHERETSLPWSEVRARAERAAANLVH
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LGIRRGDRVAIVLRTEPAFLDAFFGAWLAGAVPVPLYPPVRLGRLDAYAVDTGRMIQVAG
HHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEEECCCCEEEEEC
ARLVVSAGGTRRLLGGAVERGGPELGCVDVSALSALPARIAREPEPDALGLVQFSSGTTV
CEEEEECCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
DPKPVALTHRALAAMTDALVAATSAGPDDVLVSWLPLYHDMGLIGCLLAAMSYPGPLVLI
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
PPEHFLTRPALWLRALARRRGTISAAPSFAYAYAADRVQDADLAGLSLAPVRLLLDGAEP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHCCCEEECCCCH
VSAVALRRFAERFAPHGLDPRALVPVYGLSEAALAVTFARPGGPLGGLGVDAARLASEGV
HHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCC
VAPGGREVMPVGTPVPGVEVELRTEAGAPVGEGRIGRVFVRGPALMREYLGDPEATARAL
CCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECHHHHHHHCCCHHHHHHHH
RGGWLDTGDLGFVVDGALHLHGRARDLVIVRGANHAPEEFEAPLAALPGLRPGCAVALGF
HCCCCCCCCCCEEEECEEEECCCCCEEEEEECCCCCCHHHCCCHHHCCCCCCCCEEEEEC
EPEGGAGEALLVLAERARDEETPDGELEARVRRAVLAATGIAPHTVRLLAPGTLPRTSSG
CCCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCC
KLRRAEALRRFQAGTLTPPQPVSAIRLALAAARSQLAFGRSRRRGG
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLRGPPQPPPRHTTLVHALAAAAAHPSGATFLDLHERETSLPWSEVRARAERAAANLVH
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LGIRRGDRVAIVLRTEPAFLDAFFGAWLAGAVPVPLYPPVRLGRLDAYAVDTGRMIQVAG
HHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEECCCEEEEECCCCEEEEEC
ARLVVSAGGTRRLLGGAVERGGPELGCVDVSALSALPARIAREPEPDALGLVQFSSGTTV
CEEEEECCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
DPKPVALTHRALAAMTDALVAATSAGPDDVLVSWLPLYHDMGLIGCLLAAMSYPGPLVLI
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
PPEHFLTRPALWLRALARRRGTISAAPSFAYAYAADRVQDADLAGLSLAPVRLLLDGAEP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHCCCEEECCCCH
VSAVALRRFAERFAPHGLDPRALVPVYGLSEAALAVTFARPGGPLGGLGVDAARLASEGV
HHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCC
VAPGGREVMPVGTPVPGVEVELRTEAGAPVGEGRIGRVFVRGPALMREYLGDPEATARAL
CCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEECHHHHHHHCCCHHHHHHHH
RGGWLDTGDLGFVVDGALHLHGRARDLVIVRGANHAPEEFEAPLAALPGLRPGCAVALGF
HCCCCCCCCCCEEEECEEEECCCCCEEEEEECCCCCCHHHCCCHHHCCCCCCCCEEEEEC
EPEGGAGEALLVLAERARDEETPDGELEARVRRAVLAATGIAPHTVRLLAPGTLPRTSSG
CCCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCC
KLRRAEALRRFQAGTLTPPQPVSAIRLALAAARSQLAFGRSRRRGG
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]