Click here to switch to the map view.

The map label for this gene is rlmH

Identifier: 220915376

GI number: 220915376

Start: 276148

End: 276609

Strand: Direct

Name: rlmH

Synonym: A2cp1_0255

Alternate gene names: 220915376

Gene position: 276148-276609 (Clockwise)

Preceding gene: 220915375

Following gene: 220915377

Centisome position: 5.49

GC content: 75.32

Gene sequence:

>462_bases
GTGAAGGTCCGGGTGGTGGCGGTGGGCCGGGATCGCTCGGGCCTGTACGCGCCGGCGGTGGACGAGTACGCGAAGCGGCT
CGGCCGCTACCTGCGCTTCGAGCTGGTGGAGGTGCCGGAGGCGCGCAAGCTCGCCGGCACCGCGGGCGCGAAGGGCGAGG
AGGGCGCGACGCTCCTCGCGAAGGTCGGGCCGCGCGAGCGGGTGGTGGTGCTCGACGAGCGCGGCGACGAGCTCACCAGC
GTGGCCTTCGCCGAGCGCGTGCGCCGCTGGATGGAGCGCGGCCAGGACGTGGCGCTCCTCATCGGCGGGTCGGACGGGCT
CGCGCCGGAGGTGCTGGCCCGGGCCGACGAGCGGCTGGCGGTGTCGCGCTTCACGCTGGCGCACCGGCTGGCGCGGCTGG
TGCTCGTCGAGCAGCTCTACCGCGCCATGACCATCCTCCGCGGCGAGCCCTACCACAAGTGA

Upstream 100 bases:

>100_bases
TCTCGCGCTACCGCCAGGACGGGAACCGCGAGCTGCTCGCGAAGCTCGACGACGTGCTGGCGCGCGCGCTCGACGGCGGC
GCGCCCGGGGAGCCGCGGGC

Downstream 100 bases:

>100_bases
AACCGGCGCGCTGCCCCGCGCCCGCGGGCCCCGCGCACCCATGGGTCACGGCGGTGGGCTTGCGCGCCGCCGCCCGCGCT
GCGACAACCAGCCGACCACC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MKVRVVAVGRDRSGLYAPAVDEYAKRLGRYLRFELVEVPEARKLAGTAGAKGEEGATLLAKVGPRERVVVLDERGDELTS
VAFAERVRRWMERGQDVALLIGGSDGLAPEVLARADERLAVSRFTLAHRLARLVLVEQLYRAMTILRGEPYHK

Sequences:

>Translated_153_residues
MKVRVVAVGRDRSGLYAPAVDEYAKRLGRYLRFELVEVPEARKLAGTAGAKGEEGATLLAKVGPRERVVVLDERGDELTS
VAFAERVRRWMERGQDVALLIGGSDGLAPEVLARADERLAVSRFTLAHRLARLVLVEQLYRAMTILRGEPYHK
>Mature_153_residues
MKVRVVAVGRDRSGLYAPAVDEYAKRLGRYLRFELVEVPEARKLAGTAGAKGEEGATLLAKVGPRERVVVLDERGDELTS
VAFAERVRRWMERGQDVALLIGGSDGLAPEVLARADERLAVSRFTLAHRLARLVLVEQLYRAMTILRGEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=36.7741935483871, Blast_Score=102, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_ANAD2 (B8J916)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002490680.1
- ProteinModelPortal:   B8J916
- SMR:   B8J916
- GeneID:   7298957
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0255
- HOGENOM:   HBG622807
- ProtClustDB:   CLSK944912
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17011; Mature: 17011

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRVVAVGRDRSGLYAPAVDEYAKRLGRYLRFELVEVPEARKLAGTAGAKGEEGATLLA
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCEEEE
KVGPRERVVVLDERGDELTSVAFAERVRRWMERGQDVALLIGGSDGLAPEVLARADERLA
ECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
VSRFTLAHRLARLVLVEQLYRAMTILRGEPYHK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVRVVAVGRDRSGLYAPAVDEYAKRLGRYLRFELVEVPEARKLAGTAGAKGEEGATLLA
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCEEEE
KVGPRERVVVLDERGDELTSVAFAERVRRWMERGQDVALLIGGSDGLAPEVLARADERLA
ECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
VSRFTLAHRLARLVLVEQLYRAMTILRGEPYHK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA