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The map label for this gene is algU [H]

Identifier: 220915373

GI number: 220915373

Start: 272900

End: 273508

Strand: Direct

Name: algU [H]

Synonym: A2cp1_0252

Alternate gene names: 220915373

Gene position: 272900-273508 (Clockwise)

Preceding gene: 220915371

Following gene: 220915374

Centisome position: 5.43

GC content: 76.85

Gene sequence:

>609_bases
GTGCCGGACGAGAGCGACGAGCGGCTGATGCTCCGGTTCCGGGACGGCGACGCCCGGGCGTTCGAGGCCCTCGTGCGCCG
CCACCGGACGCCGATCTTCTCCTTCCTGCTCCGCCTGACCCGCGATCGCGGCCGGGCGGAGGACCTCTGCCAGGACACGT
TCCTGCGGGTGGTGAAGGCTTCCGGCGCCTGGGAGCCGCGCGCCCGCTTCCGCACCTGGCTCTACGCGATGGCCCGCAAC
CTGGCGGTGGACGAGTCGCGGCGCATGGCGTTCCGCCGCGCCAGCTCGCTCGAGGCGGACCCCGCCGCCTCCGCCCTGGC
CGCGGACGATCCGGCGCCCGACCGCGCGGCCGACGCGGCGCTCGTCCGCCCGCTCCTCGAGGCGGCGCTCGCGTCGCTGC
CCGAGGAGCAGCGCGAGGTGTTCCTGCTGCGCGAGCACGCGGGGCTCAGGTTCCCGGAGATCGCCGAGGTCACCGGCGCG
CCGGAGCCGACCGTGAAGAGCCGGATGCGCTACGCGCTCGAGGCGCTGCGCGAGCGGCTCGCGGCGATGGGCGTGGAGCC
GGAGGTGGCCGCGGGCGGCCCCGCCTCGACCCGGAGCGCGGCACCATGA

Upstream 100 bases:

>100_bases
GGGTCACCCGACCGGAGCGGCCCGTTTCGACTACACTCCGGCGCCCGAGATCCTTTTCCCGCCTCGCGCGTTCCCCCGGC
GAGGCCCAGGCGACACGCGC

Downstream 100 bases:

>100_bases
GCGACGAACGCTTCCACGAGCGCCTCGGGGCGCGGCTGCACGACGCCATCCTGGAGCACGCGTACGGCGAGCTGCCGGCG
GGCGAGGGGCGGGCGCTGGA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MPDESDERLMLRFRDGDARAFEALVRRHRTPIFSFLLRLTRDRGRAEDLCQDTFLRVVKASGAWEPRARFRTWLYAMARN
LAVDESRRMAFRRASSLEADPAASALAADDPAPDRAADAALVRPLLEAALASLPEEQREVFLLREHAGLRFPEIAEVTGA
PEPTVKSRMRYALEALRERLAAMGVEPEVAAGGPASTRSAAP

Sequences:

>Translated_202_residues
MPDESDERLMLRFRDGDARAFEALVRRHRTPIFSFLLRLTRDRGRAEDLCQDTFLRVVKASGAWEPRARFRTWLYAMARN
LAVDESRRMAFRRASSLEADPAASALAADDPAPDRAADAALVRPLLEAALASLPEEQREVFLLREHAGLRFPEIAEVTGA
PEPTVKSRMRYALEALRERLAAMGVEPEVAAGGPASTRSAAP
>Mature_201_residues
PDESDERLMLRFRDGDARAFEALVRRHRTPIFSFLLRLTRDRGRAEDLCQDTFLRVVKASGAWEPRARFRTWLYAMARNL
AVDESRRMAFRRASSLEADPAASALAADDPAPDRAADAALVRPLLEAALASLPEEQREVFLLREHAGLRFPEIAEVTGAP
EPTVKSRMRYALEALRERLAAMGVEPEVAAGGPASTRSAAP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=181, Percent_Identity=29.8342541436464, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22471; Mature: 22340

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDESDERLMLRFRDGDARAFEALVRRHRTPIFSFLLRLTRDRGRAEDLCQDTFLRVVKA
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SGAWEPRARFRTWLYAMARNLAVDESRRMAFRRASSLEADPAASALAADDPAPDRAADAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHH
LVRPLLEAALASLPEEQREVFLLREHAGLRFPEIAEVTGAPEPTVKSRMRYALEALRERL
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAMGVEPEVAAGGPASTRSAAP
HHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDESDERLMLRFRDGDARAFEALVRRHRTPIFSFLLRLTRDRGRAEDLCQDTFLRVVKA
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SGAWEPRARFRTWLYAMARNLAVDESRRMAFRRASSLEADPAASALAADDPAPDRAADAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHH
LVRPLLEAALASLPEEQREVFLLREHAGLRFPEIAEVTGAPEPTVKSRMRYALEALRERL
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAMGVEPEVAAGGPASTRSAAP
HHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]