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The map label for this gene is nolF [H]

Identifier: 220915368

GI number: 220915368

Start: 265374

End: 266516

Strand: Direct

Name: nolF [H]

Synonym: A2cp1_0247

Alternate gene names: 220915368

Gene position: 265374-266516 (Clockwise)

Preceding gene: 220915366

Following gene: 220915369

Centisome position: 5.28

GC content: 77.43

Gene sequence:

>1143_bases
ATGCCGAGAGCCACCGGACCTCGACACGGATGCAGGCTGCTCGGGCTGGCGCTCGCCGCGGCGGTCGGCCTGGCCGGCTG
CAAGCGAGACGGCGGCAGGGCGGCCGAGGCCGCGCAGGAGCAGCCTCCGGTCGCGCTCGCGCCGGAGAACGTCGCGGTGG
TGTCGTCCCGCCGTCTGCAGTCCGGCCCGGAGGTGGCCGGCACGCTGCACGCCCGCCGCACGGCCACGCTGCGCGCCGAG
GTGGGCGGCGCCGTCGCCGAGGTCCGCGTCGACGCGGGCGAGCGGGTGAAGTCGGGCCAGCTCCTCGCCCGGATCGACGC
CTCGGCGCTGCAGGACGCGGTGCGGGCGGCCCACTCCGCGGTGACCTCGGCGCAGAACGCGCTGCGCGTGGCGGAGTCCG
GCGCCCGGCGCGCACACACGCTGGCGCGCGAGGGCGCCATGGCCGAGCAGGACGCGGAGCGCGCCGACGCGCAGCTCGAG
GCGGCGCGCGCGCAGGTCGAGGACGCGAAGGCCCGGCTCGCCGCGGCCGAGCAGCAGGCCGGCAAGACCGCCGTGCGCGC
GCCGTTCGCCGGCGTGGTCTCGGAGCGGAGCGTCTCGTCCGGCGACGTGGTGGCGTCCGGCGCGGCGCTCTACACGGTGA
TCGATCCCTCGCGGCTCCAGTTCGAGGGCTCGGTGCCCGCGGCGAGCGTGGGCGCGCTCGTGCCGGGCGCGCCGGTGGAC
TTCTCGGTGACCGGCTTCGGCGGCCGCCGCTTCGACGGCCACATCGAGCGCGTGAACCCCGCCGTCGATCCCAGCACCGG
CCAGGTGCGCGTCTACGTGGACGTGCCGAACGCCGACGGCCGGCTCCTCGCCGGCCTCTACGCGCAGGGGCGGGTGGCGA
GCCAGGCGAGCGAGGCCCTGTCGGCGCCGGCGACCGCGGTCGACGCGACCTCCACCCCGCCCACCGTCATGAAGGTGCAG
GACGGGAAGGTGCAGAAGGTGGCGGTCGAGATCGCGCTCCGCGACGACGTGGCCGGCGCGGTGGGCTTCCGCTCCGGCGT
GAAGCAGGGCGACGTGCTCGTGCTCGGCTCGGCCCGCGGCGCGCTCGCCGACGGCACCCCGGTCCGCGTGGTCGAGGACC
CGCGCGCGCAGAAGTCGAACTAG

Upstream 100 bases:

>100_bases
CCGCGCGCGGGAGCCCAAGCTGGGCCCAGCGCCTGTGAAGACCGCAGCGTAACTCGTTCCGGGCCGAGATCGCCCGGCAG
GTCGTCGGAAGGGAAACGCG

Downstream 100 bases:

>100_bases
CCGCCCCGGGCGCTCCGCATGTTCATCTCCGACTTCGCCATCAAGCGGCCCATCGTCACCATCACGGTGATGCTGGCGCT
CGTGATCTTCGGGCTCCTGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPRATGPRHGCRLLGLALAAAVGLAGCKRDGGRAAEAAQEQPPVALAPENVAVVSSRRLQSGPEVAGTLHARRTATLRAE
VGGAVAEVRVDAGERVKSGQLLARIDASALQDAVRAAHSAVTSAQNALRVAESGARRAHTLAREGAMAEQDAERADAQLE
AARAQVEDAKARLAAAEQQAGKTAVRAPFAGVVSERSVSSGDVVASGAALYTVIDPSRLQFEGSVPAASVGALVPGAPVD
FSVTGFGGRRFDGHIERVNPAVDPSTGQVRVYVDVPNADGRLLAGLYAQGRVASQASEALSAPATAVDATSTPPTVMKVQ
DGKVQKVAVEIALRDDVAGAVGFRSGVKQGDVLVLGSARGALADGTPVRVVEDPRAQKSN

Sequences:

>Translated_380_residues
MPRATGPRHGCRLLGLALAAAVGLAGCKRDGGRAAEAAQEQPPVALAPENVAVVSSRRLQSGPEVAGTLHARRTATLRAE
VGGAVAEVRVDAGERVKSGQLLARIDASALQDAVRAAHSAVTSAQNALRVAESGARRAHTLAREGAMAEQDAERADAQLE
AARAQVEDAKARLAAAEQQAGKTAVRAPFAGVVSERSVSSGDVVASGAALYTVIDPSRLQFEGSVPAASVGALVPGAPVD
FSVTGFGGRRFDGHIERVNPAVDPSTGQVRVYVDVPNADGRLLAGLYAQGRVASQASEALSAPATAVDATSTPPTVMKVQ
DGKVQKVAVEIALRDDVAGAVGFRSGVKQGDVLVLGSARGALADGTPVRVVEDPRAQKSN
>Mature_379_residues
PRATGPRHGCRLLGLALAAAVGLAGCKRDGGRAAEAAQEQPPVALAPENVAVVSSRRLQSGPEVAGTLHARRTATLRAEV
GGAVAEVRVDAGERVKSGQLLARIDASALQDAVRAAHSAVTSAQNALRVAESGARRAHTLAREGAMAEQDAERADAQLEA
ARAQVEDAKARLAAAEQQAGKTAVRAPFAGVVSERSVSSGDVVASGAALYTVIDPSRLQFEGSVPAASVGALVPGAPVDF
SVTGFGGRRFDGHIERVNPAVDPSTGQVRVYVDVPNADGRLLAGLYAQGRVASQASEALSAPATAVDATSTPPTVMKVQD
GKVQKVAVEIALRDDVAGAVGFRSGVKQGDVLVLGSARGALADGTPVRVVEDPRAQKSN

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=323, Percent_Identity=26.625386996904, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789929, Length=295, Percent_Identity=27.7966101694915, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081951, Length=183, Percent_Identity=26.2295081967213, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082036, Length=319, Percent_Identity=25.0783699059561, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38676; Mature: 38545

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRATGPRHGCRLLGLALAAAVGLAGCKRDGGRAAEAAQEQPPVALAPENVAVVSSRRLQ
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCEEEEEHHHHC
SGPEVAGTLHARRTATLRAEVGGAVAEVRVDAGERVKSGQLLARIDASALQDAVRAAHSA
CCCCHHHHHHHHHHEEEHHHHCCEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHH
VTSAQNALRVAESGARRAHTLAREGAMAEQDAERADAQLEAARAQVEDAKARLAAAEQQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKTAVRAPFAGVVSERSVSSGDVVASGAALYTVIDPSRLQFEGSVPAASVGALVPGAPVD
CCHHHHCCHHHHHHCCCCCCCCEEECCEEEEEEECCCCEEECCCCCCHHHCCCCCCCCCC
FSVTGFGGRRFDGHIERVNPAVDPSTGQVRVYVDVPNADGRLLAGLYAQGRVASQASEAL
EEEECCCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEHHHCCCHHHHHHHHH
SAPATAVDATSTPPTVMKVQDGKVQKVAVEIALRDDVAGAVGFRSGVKQGDVLVLGSARG
CCCCCEECCCCCCCEEEEECCCCEEEEEEEEEECCCCHHHHHHHCCCCCCCEEEEECCCC
ALADGTPVRVVEDPRAQKSN
CCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
PRATGPRHGCRLLGLALAAAVGLAGCKRDGGRAAEAAQEQPPVALAPENVAVVSSRRLQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCEEEEEHHHHC
SGPEVAGTLHARRTATLRAEVGGAVAEVRVDAGERVKSGQLLARIDASALQDAVRAAHSA
CCCCHHHHHHHHHHEEEHHHHCCEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHH
VTSAQNALRVAESGARRAHTLAREGAMAEQDAERADAQLEAARAQVEDAKARLAAAEQQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKTAVRAPFAGVVSERSVSSGDVVASGAALYTVIDPSRLQFEGSVPAASVGALVPGAPVD
CCHHHHCCHHHHHHCCCCCCCCEEECCEEEEEEECCCCEEECCCCCCHHHCCCCCCCCCC
FSVTGFGGRRFDGHIERVNPAVDPSTGQVRVYVDVPNADGRLLAGLYAQGRVASQASEAL
EEEECCCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEHHHCCCHHHHHHHHH
SAPATAVDATSTPPTVMKVQDGKVQKVAVEIALRDDVAGAVGFRSGVKQGDVLVLGSARG
CCCCCEECCCCCCCEEEEECCCCEEEEEEEEEECCCCHHHHHHHCCCCCCCEEEEECCCC
ALADGTPVRVVEDPRAQKSN
CCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]