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The map label for this gene is manX [H]

Identifier: 220915288

GI number: 220915288

Start: 184617

End: 185111

Strand: Direct

Name: manX [H]

Synonym: A2cp1_0167

Alternate gene names: 220915288

Gene position: 184617-185111 (Clockwise)

Preceding gene: 220915287

Following gene: 220915289

Centisome position: 3.67

GC content: 73.94

Gene sequence:

>495_bases
GTGATCCCCCTGGTCCGCGTCGACAACCGCCTGCTGCACGGCCAGATCCTCGAGACCTGGGTGCCCCGCCTGCGCATCGA
CCAGGTGATGGTCGCGGACGACGAGGCGGCCGCCAGCTCGCTCGCGCAGGCGGCCATGACCCTGTGCGTCCCGCCGGAGC
TGCCGGTGCGCATCCGCCGGGTGGCGGAGGTCGACTACGCGGGGCTGGCCGCGGGCGGCGCCAAGGTGCTCCTGCTGGTG
CGCGACGTGCCCGGGCTGGCGGCGGCGCGCGCCGCCGGCCTCGGCGCCGCGCTCGCACCGCGGGTGAATCTGGGCAACCT
CCACTTCGGGCCGGGGCGGCGCCTGGTCACGCCCTCGGTGTTCGTGTCGGAGGAGGACCTGTCGGTCCTGCGCCGGCTTG
CCGCGGAAGGCTTCGAGGTGGAAGCTCGCGCGATTCCTTCCGAATCCCCCACGGGTTTGGTCGAGATCGAACGCCGGTAC
GCCGCGGGCCATTAG

Upstream 100 bases:

>100_bases
AAGAACATCACCCTCGCCTCCGAGCTGCTGCGCACGCGCGCGCGGAGCCGGACGTAGCCGACCGCTGAATGGCCGGCCGG
GAGCGACCGTCGAGTCTCAC

Downstream 100 bases:

>100_bases
GTTACACTCGTCCGGCTTGGGTCTTCTCTTCCTAGGGCTCGTGGCGGGCCTCGCCGCCGTGGAGCGCAAGGGGTTCCTGC
AGGCCATGCTGTCGCGCCCC

Product: PTS system sorbose subfamily IIB component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MIPLVRVDNRLLHGQILETWVPRLRIDQVMVADDEAAASSLAQAAMTLCVPPELPVRIRRVAEVDYAGLAAGGAKVLLLV
RDVPGLAAARAAGLGAALAPRVNLGNLHFGPGRRLVTPSVFVSEEDLSVLRRLAAEGFEVEARAIPSESPTGLVEIERRY
AAGH

Sequences:

>Translated_164_residues
MIPLVRVDNRLLHGQILETWVPRLRIDQVMVADDEAAASSLAQAAMTLCVPPELPVRIRRVAEVDYAGLAAGGAKVLLLV
RDVPGLAAARAAGLGAALAPRVNLGNLHFGPGRRLVTPSVFVSEEDLSVLRRLAAEGFEVEARAIPSESPTGLVEIERRY
AAGH
>Mature_164_residues
MIPLVRVDNRLLHGQILETWVPRLRIDQVMVADDEAAASSLAQAAMTLCVPPELPVRIRRVAEVDYAGLAAGGAKVLLLV
RDVPGLAAARAAGLGAALAPRVNLGNLHFGPGRRLVTPSVFVSEEDLSVLRRLAAEGFEVEARAIPSESPTGLVEIERRY
AAGH

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=161, Percent_Identity=27.9503105590062, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI87082217, Length=156, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789527, Length=150, Percent_Identity=29.3333333333333, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17470; Mature: 17470

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLVRVDNRLLHGQILETWVPRLRIDQVMVADDEAAASSLAQAAMTLCVPPELPVRIRR
CCCEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHH
VAEVDYAGLAAGGAKVLLLVRDVPGLAAARAAGLGAALAPRVNLGNLHFGPGRRLVTPSV
HHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHCCCCHHCCCCCCCCEEECCCCEEECCEE
FVSEEDLSVLRRLAAEGFEVEARAIPSESPTGLVEIERRYAAGH
EECHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEHHHHCCCC
>Mature Secondary Structure
MIPLVRVDNRLLHGQILETWVPRLRIDQVMVADDEAAASSLAQAAMTLCVPPELPVRIRR
CCCEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHH
VAEVDYAGLAAGGAKVLLLVRDVPGLAAARAAGLGAALAPRVNLGNLHFGPGRRLVTPSV
HHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHCCCCHHCCCCCCCCEEECCCCEEECCEE
FVSEEDLSVLRRLAAEGFEVEARAIPSESPTGLVEIERRYAAGH
EECHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]