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The map label for this gene is ogt [H]

Identifier: 220915238

GI number: 220915238

Start: 131972

End: 132457

Strand: Direct

Name: ogt [H]

Synonym: A2cp1_0117

Alternate gene names: 220915238

Gene position: 131972-132457 (Clockwise)

Preceding gene: 220915237

Following gene: 220915239

Centisome position: 2.62

GC content: 78.81

Gene sequence:

>486_bases
GTGACCGCGCGCCTGCGCTACCGCTCGCCGGTGGGGATCCTCACGGTCGAGGCGAGCGACGAGGGGGTGTTCGGCCTTTC
GTTCGGCGATCGCGGGCCGTCGCGCCCCGGCCCGTCGGCGCGTGCGCGCGCCCACCTCGACGCGGCGGCGCGGGCGCTCG
ACGACTACTTCGCGGGCCGGCCGCCCTCGCTCCCGCCGCTCGTCCTCCAGGGGAGCCCGTTCCAGAAGCGTGTCTGGGAC
GCGCTCCTCGCCATCCCGTGGGGCGACACCCGCACCTACGGCGAGGTGGCCGAGGCGCTCGGCGCCGCGGGCGGCGCCCG
GGCGGTGGGCGGCGCGAACCACGAGAACCCGGTCGCGATCCTGGTGCCGTGCCACCGGGTGGTGCAGGCCCGCGGGCGGC
TGGGCGGCTACGGCGGCGGCGTGGAGGTGAAGCGCTGGCTGCTCGCGCACGAGGCGGCGCACGGCCCGGCGCTCCGGCCG
GCGTGA

Upstream 100 bases:

>100_bases
GGGTGAACCTGGAGATCGCGCAGGTGCTCGCGCGCCACGGCGCGCCGGCGCCGGTCGCGGGCGACGGCGCGGCCCGCGCG
GCGGTCGAGGCGGGGACGCC

Downstream 100 bases:

>100_bases
CCTCGCCGCGCCGCCCCCCCGACGAGGCGCGCGCGGGGGCGCAGCGCGGGCCGGGATCGCGCCGCCGCCCGCGCGCCCGC
TTCCTTGCCCTGCCCCGAGC

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTARLRYRSPVGILTVEASDEGVFGLSFGDRGPSRPGPSARARAHLDAAARALDDYFAGRPPSLPPLVLQGSPFQKRVWD
ALLAIPWGDTRTYGEVAEALGAAGGARAVGGANHENPVAILVPCHRVVQARGRLGGYGGGVEVKRWLLAHEAAHGPALRP
A

Sequences:

>Translated_161_residues
MTARLRYRSPVGILTVEASDEGVFGLSFGDRGPSRPGPSARARAHLDAAARALDDYFAGRPPSLPPLVLQGSPFQKRVWD
ALLAIPWGDTRTYGEVAEALGAAGGARAVGGANHENPVAILVPCHRVVQARGRLGGYGGGVEVKRWLLAHEAAHGPALRP
A
>Mature_160_residues
TARLRYRSPVGILTVEASDEGVFGLSFGDRGPSRPGPSARARAHLDAAARALDDYFAGRPPSLPPLVLQGSPFQKRVWDA
LLAIPWGDTRTYGEVAEALGAAGGARAVGGANHENPVAILVPCHRVVQARGRLGGYGGGVEVKRWLLAHEAAHGPALRPA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=164, Percent_Identity=34.1463414634146, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1787596, Length=101, Percent_Identity=50.4950495049505, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788542, Length=93, Percent_Identity=45.1612903225806, Blast_Score=83, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=83, Percent_Identity=40.9638554216867, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533068, Length=83, Percent_Identity=40.9638554216867, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=96, Percent_Identity=38.5416666666667, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16887; Mature: 16756

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARLRYRSPVGILTVEASDEGVFGLSFGDRGPSRPGPSARARAHLDAAARALDDYFAGR
CCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PPSLPPLVLQGSPFQKRVWDALLAIPWGDTRTYGEVAEALGAAGGARAVGGANHENPVAI
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEE
LVPCHRVVQARGRLGGYGGGVEVKRWLLAHEAAHGPALRPA
EECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TARLRYRSPVGILTVEASDEGVFGLSFGDRGPSRPGPSARARAHLDAAARALDDYFAGR
CCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
PPSLPPLVLQGSPFQKRVWDALLAIPWGDTRTYGEVAEALGAAGGARAVGGANHENPVAI
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEE
LVPCHRVVQARGRLGGYGGGVEVKRWLLAHEAAHGPALRPA
EECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA