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The map label for this gene is pcrA [H]

Identifier: 220915236

GI number: 220915236

Start: 128355

End: 130748

Strand: Direct

Name: pcrA [H]

Synonym: A2cp1_0115

Alternate gene names: 220915236

Gene position: 128355-130748 (Clockwise)

Preceding gene: 220915235

Following gene: 220915237

Centisome position: 2.55

GC content: 76.19

Gene sequence:

>2394_bases
TTGGGCTATCTTCCGGCCCCCGTGGCCGAGGACCTGCTCCAGGATCTCAACGACGCCCAGCGCGAGGCGGTGCTGCACGA
CGACGGCCCGCTGCTCGTGCTCGCCGGCGCGGGCAGCGGCAAGACGCGCGTCATCGTGCACCGCATCGCCCGGCTGGTGC
GCGATCGCGGGGTGGTGCCCTGGCACGTGCTCGCGGTCACGTTCACCAACAAGGCCGCCGGCGAGATGCGCGAGCGGCTC
GAGCGCCTGCTCGGCCCGCTGGCGCGCGAGCTGTGGGTCCAGACCTTCCACGCGTTCGGCGCCCGCTTCCTGCGCCGGGA
GGCCGCCCGGGCCGGCCTCTCGCCCTCGTTCGCGATCTACGACGACGACGACCAGCTCCGGCTGGTGAAGGGGCTCCTCT
CCGAGCTGGGCCTCGACGAGGGCGAGGGGCTCACCCCGCGCCAGGTCCTCTCGCAGATCGACCGCTGGAAGAACCAGGCG
CTCCGCGCCGCCGAGGTGAAGGTCGCCGGGCTCGATCCGGAGGCCGCGCGCACGAAGGAGGTCTACCACCGCTACGAGCA
GGCGCTGGCGCGCGCGGGCGCGGTGGACTTCGGCGACCTGCTGCTCCGGCCGGTGCGGCTGCTGGAGGAGGACGCGGCGC
TGCGCGCCCGGTGGGCCGGCCGCTTCCGCTACCTGCTGGTGGACGAGTTCCAGGACACGAACCCGGTCCAGTACCGGCTG
CTGCAGCTCCTCACCGGCCCGCGCCGCAACGTCTGCGTGGTGGGCGACGACGACCAGGCCATCTACCGCTGGCGCGGCGC
CGACGTCTCCAACATCCTCGGCTTCGACGGCGACTTCCCGGGCACGAAGGTGGTGAAGCTGGAGCAGAACTACCGCTCCA
CCCGCACCATCCTCGACGCGGCGCACGCGGTGATCTCGCGGGCCCGGCGCCGGCGCGACAAGCGGCTCTGGACCGAGTCC
GGGGCGGGCGCGCCGCTGGCGCTGCTGGTGGGAGAGGACGAGCACGCCGAGGCCGAGCGGATCGCGCGGGCGGTCGCGGC
GGAGCGCGGCCGCGGCACCTCCGGCGACGAGATCGCGGTGCTGTACCGGACGAACGCGCAATCGCGGCCGCTCGAGGCGG
CCATGCGGGCGGCGCGCATCCCGTACGTCATCGTCCGCGGCACGAGCTTCTACGAGCGGGCCGAGGTGCGGGACGCGGCC
GCGTACCTGCGCCTCGCGCTCTCGCCCGCGTCCGACCTCGACCTCGAGCGGGTGGTGAACCGGCCGCCGCGCGGCATCGG
GGACAAGACGGTGGAGCGGCTGCGGGCCCACGCCGCCGCCCGGGGCCTGCCGCTCGTCGAGGCGGTGGCGGAGCGCGACG
CGGTGGCGGAGCTGAAGCCGGCCGCGCGCCGGGCGCTGGCCGAGTTCCACGCGCTCGTCACCGGCCTCGCCGCCGACGTC
CCGGCGCTCGACGCCGGCGTGGCGGTGCAGGAGGTGCTGAAGCGCTCCGGCATGCTGGCGCGCCTCGAGGAGGAGCACAC
CGACGAGGCGGTGGAGCGGGCCGAGAACCTGGCCGAGCTGGTGGCCGCCGCGCGCGAGTTCGACGAGTCCATCGCCGGCG
AGCCGCCGCCCGGCGATCCGGAGGAGGTGCGCCCGCCGCCGCTGGCCCGCTTCCTCGAGCAGATCGCGCTGCTCGGCGAG
GCCGACGCCGCCACGCCGGAGGGGCGCGTCGCGCTCATGACGCTGCACGCGGCGAAGGGGCTCGAGTTCGAGGCGGTGTT
CCTGGCCGGCATGGAGGACGGGACGCTGCCCTACGAGCGGCCCTGGGCCGACCAGACCGCGTCGGAGCGGGACGAGTCGC
TCGACGAGGAGCGGCGCCTCTGCTACGTCGGCATGACCCGCGCCAAGCGGCGCCTCACGATCTCGCTGGCGCGGCGGCGC
ATCGGCTACGGCGAGAACGGCCCCTCCTACCGCAACATGGAGCCCTCGCGCTTCCTGCGCGACCTGCCGCCGGAGCTGTT
CGGCGACGCGGTGGCGCGCGAGGTGCGCGCGCGCGAGGCGGCCCGGGCGGCGCCGGTGGCGCGCCCGCCCACCATCCGGC
GGCTCCCCGGCGCGCTCGAGGGCGAGCCGCACATCGAGCTCGACGGCGACGAGCTCTCGCAGCCGGCCGCGCCCGCGCCG
CGCGCCCGCCCGGCGGCGCCGCGCGGCGAGCCCACGGTGGACTACTCGTTCGACCAGCGCGGCGACGCCGGCGCGGCCGC
CTTCTCGCGGGGCGAGCGGGTGGCGCACGCCTCGCTCGGCGAGGGCGTGGTGCTCGCCTGCGACGCGGGCGGCGCGGACG
CGAAGGCCACCGTGCGGTTCGCCTCGGCCGGCGACAAGCGCGTCCTGGCGCGGTTCCTCCGGAGAGCCTTCTAG

Upstream 100 bases:

>100_bases
CGCCGCCGCGTCCCGGGCACGCCGCTCGAGCCCGCCCCGGTGCCAGCCCCGGTGCCCGTGCAGTTGCCGGCGGCGTAGAC
CCCCTGCGGCCGCCGCCGCG

Downstream 100 bases:

>100_bases
CGCGCCTGCCCCGTTCGCCGGGCGCGGGCCCCACCTCGCCGGGCGGCGCCCCCACCCGCCCCTCCCTCCGAGGCGGCGGC
GGCGTATGATCCGGGCATGG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 797; Mature: 796

Protein sequence:

>797_residues
MGYLPAPVAEDLLQDLNDAQREAVLHDDGPLLVLAGAGSGKTRVIVHRIARLVRDRGVVPWHVLAVTFTNKAAGEMRERL
ERLLGPLARELWVQTFHAFGARFLRREAARAGLSPSFAIYDDDDQLRLVKGLLSELGLDEGEGLTPRQVLSQIDRWKNQA
LRAAEVKVAGLDPEAARTKEVYHRYEQALARAGAVDFGDLLLRPVRLLEEDAALRARWAGRFRYLLVDEFQDTNPVQYRL
LQLLTGPRRNVCVVGDDDQAIYRWRGADVSNILGFDGDFPGTKVVKLEQNYRSTRTILDAAHAVISRARRRRDKRLWTES
GAGAPLALLVGEDEHAEAERIARAVAAERGRGTSGDEIAVLYRTNAQSRPLEAAMRAARIPYVIVRGTSFYERAEVRDAA
AYLRLALSPASDLDLERVVNRPPRGIGDKTVERLRAHAAARGLPLVEAVAERDAVAELKPAARRALAEFHALVTGLAADV
PALDAGVAVQEVLKRSGMLARLEEEHTDEAVERAENLAELVAAAREFDESIAGEPPPGDPEEVRPPPLARFLEQIALLGE
ADAATPEGRVALMTLHAAKGLEFEAVFLAGMEDGTLPYERPWADQTASERDESLDEERRLCYVGMTRAKRRLTISLARRR
IGYGENGPSYRNMEPSRFLRDLPPELFGDAVAREVRAREAARAAPVARPPTIRRLPGALEGEPHIELDGDELSQPAAPAP
RARPAAPRGEPTVDYSFDQRGDAGAAAFSRGERVAHASLGEGVVLACDAGGADAKATVRFASAGDKRVLARFLRRAF

Sequences:

>Translated_797_residues
MGYLPAPVAEDLLQDLNDAQREAVLHDDGPLLVLAGAGSGKTRVIVHRIARLVRDRGVVPWHVLAVTFTNKAAGEMRERL
ERLLGPLARELWVQTFHAFGARFLRREAARAGLSPSFAIYDDDDQLRLVKGLLSELGLDEGEGLTPRQVLSQIDRWKNQA
LRAAEVKVAGLDPEAARTKEVYHRYEQALARAGAVDFGDLLLRPVRLLEEDAALRARWAGRFRYLLVDEFQDTNPVQYRL
LQLLTGPRRNVCVVGDDDQAIYRWRGADVSNILGFDGDFPGTKVVKLEQNYRSTRTILDAAHAVISRARRRRDKRLWTES
GAGAPLALLVGEDEHAEAERIARAVAAERGRGTSGDEIAVLYRTNAQSRPLEAAMRAARIPYVIVRGTSFYERAEVRDAA
AYLRLALSPASDLDLERVVNRPPRGIGDKTVERLRAHAAARGLPLVEAVAERDAVAELKPAARRALAEFHALVTGLAADV
PALDAGVAVQEVLKRSGMLARLEEEHTDEAVERAENLAELVAAAREFDESIAGEPPPGDPEEVRPPPLARFLEQIALLGE
ADAATPEGRVALMTLHAAKGLEFEAVFLAGMEDGTLPYERPWADQTASERDESLDEERRLCYVGMTRAKRRLTISLARRR
IGYGENGPSYRNMEPSRFLRDLPPELFGDAVAREVRAREAARAAPVARPPTIRRLPGALEGEPHIELDGDELSQPAAPAP
RARPAAPRGEPTVDYSFDQRGDAGAAAFSRGERVAHASLGEGVVLACDAGGADAKATVRFASAGDKRVLARFLRRAF
>Mature_796_residues
GYLPAPVAEDLLQDLNDAQREAVLHDDGPLLVLAGAGSGKTRVIVHRIARLVRDRGVVPWHVLAVTFTNKAAGEMRERLE
RLLGPLARELWVQTFHAFGARFLRREAARAGLSPSFAIYDDDDQLRLVKGLLSELGLDEGEGLTPRQVLSQIDRWKNQAL
RAAEVKVAGLDPEAARTKEVYHRYEQALARAGAVDFGDLLLRPVRLLEEDAALRARWAGRFRYLLVDEFQDTNPVQYRLL
QLLTGPRRNVCVVGDDDQAIYRWRGADVSNILGFDGDFPGTKVVKLEQNYRSTRTILDAAHAVISRARRRRDKRLWTESG
AGAPLALLVGEDEHAEAERIARAVAAERGRGTSGDEIAVLYRTNAQSRPLEAAMRAARIPYVIVRGTSFYERAEVRDAAA
YLRLALSPASDLDLERVVNRPPRGIGDKTVERLRAHAAARGLPLVEAVAERDAVAELKPAARRALAEFHALVTGLAADVP
ALDAGVAVQEVLKRSGMLARLEEEHTDEAVERAENLAELVAAAREFDESIAGEPPPGDPEEVRPPPLARFLEQIALLGEA
DAATPEGRVALMTLHAAKGLEFEAVFLAGMEDGTLPYERPWADQTASERDESLDEERRLCYVGMTRAKRRLTISLARRRI
GYGENGPSYRNMEPSRFLRDLPPELFGDAVAREVRAREAARAAPVARPPTIRRLPGALEGEPHIELDGDELSQPAAPAPR
ARPAAPRGEPTVDYSFDQRGDAGAAAFSRGERVAHASLGEGVVLACDAGGADAKATVRFASAGDKRVLARFLRRAF

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=784, Percent_Identity=39.9234693877551, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI48994965, Length=657, Percent_Identity=40.0304414003044, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1787196, Length=333, Percent_Identity=29.7297297297297, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=730, Percent_Identity=26.986301369863, Blast_Score=205, Evalue=2e-53,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 87414; Mature: 87283

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYLPAPVAEDLLQDLNDAQREAVLHDDGPLLVLAGAGSGKTRVIVHRIARLVRDRGVVP
CCCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
WHVLAVTFTNKAAGEMRERLERLLGPLARELWVQTFHAFGARFLRREAARAGLSPSFAIY
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
DDDDQLRLVKGLLSELGLDEGEGLTPRQVLSQIDRWKNQALRAAEVKVAGLDPEAARTKE
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHH
VYHRYEQALARAGAVDFGDLLLRPVRLLEEDAALRARWAGRFRYLLVDEFQDTNPVQYRL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
LQLLTGPRRNVCVVGDDDQAIYRWRGADVSNILGFDGDFPGTKVVKLEQNYRSTRTILDA
HHHHCCCCCCEEEECCCCCEEEEECCCCHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHH
AHAVISRARRRRDKRLWTESGAGAPLALLVGEDEHAEAERIARAVAAERGRGTSGDEIAV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEE
LYRTNAQSRPLEAAMRAARIPYVIVRGTSFYERAEVRDAAAYLRLALSPASDLDLERVVN
EEECCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
RPPRGIGDKTVERLRAHAAARGLPLVEAVAERDAVAELKPAARRALAEFHALVTGLAADV
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALDAGVAVQEVLKRSGMLARLEEEHTDEAVERAENLAELVAAAREFDESIAGEPPPGDP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EEVRPPPLARFLEQIALLGEADAATPEGRVALMTLHAAKGLEFEAVFLAGMEDGTLPYER
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHCCCCEEEEEEECCCCCCCCCCC
PWADQTASERDESLDEERRLCYVGMTRAKRRLTISLARRRIGYGENGPSYRNMEPSRFLR
CCCCCCHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
DLPPELFGDAVAREVRAREAARAAPVARPPTIRRLPGALEGEPHIELDGDELSQPAAPAP
HCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEECCHHCCCCCCCCC
RARPAAPRGEPTVDYSFDQRGDAGAAAFSRGERVAHASLGEGVVLACDAGGADAKATVRF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCEEEEECCCCCCCEEEEEE
ASAGDKRVLARFLRRAF
ECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GYLPAPVAEDLLQDLNDAQREAVLHDDGPLLVLAGAGSGKTRVIVHRIARLVRDRGVVP
CCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
WHVLAVTFTNKAAGEMRERLERLLGPLARELWVQTFHAFGARFLRREAARAGLSPSFAIY
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
DDDDQLRLVKGLLSELGLDEGEGLTPRQVLSQIDRWKNQALRAAEVKVAGLDPEAARTKE
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHH
VYHRYEQALARAGAVDFGDLLLRPVRLLEEDAALRARWAGRFRYLLVDEFQDTNPVQYRL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
LQLLTGPRRNVCVVGDDDQAIYRWRGADVSNILGFDGDFPGTKVVKLEQNYRSTRTILDA
HHHHCCCCCCEEEECCCCCEEEEECCCCHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHH
AHAVISRARRRRDKRLWTESGAGAPLALLVGEDEHAEAERIARAVAAERGRGTSGDEIAV
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEE
LYRTNAQSRPLEAAMRAARIPYVIVRGTSFYERAEVRDAAAYLRLALSPASDLDLERVVN
EEECCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
RPPRGIGDKTVERLRAHAAARGLPLVEAVAERDAVAELKPAARRALAEFHALVTGLAADV
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALDAGVAVQEVLKRSGMLARLEEEHTDEAVERAENLAELVAAAREFDESIAGEPPPGDP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EEVRPPPLARFLEQIALLGEADAATPEGRVALMTLHAAKGLEFEAVFLAGMEDGTLPYER
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHCCCCEEEEEEECCCCCCCCCCC
PWADQTASERDESLDEERRLCYVGMTRAKRRLTISLARRRIGYGENGPSYRNMEPSRFLR
CCCCCCHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
DLPPELFGDAVAREVRAREAARAAPVARPPTIRRLPGALEGEPHIELDGDELSQPAAPAP
HCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEECCHHCCCCCCCCC
RARPAAPRGEPTVDYSFDQRGDAGAAAFSRGERVAHASLGEGVVLACDAGGADAKATVRF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCEEEEECCCCCCCEEEEEE
ASAGDKRVLARFLRRAF
ECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]