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The map label for this gene is thlA [H]

Identifier: 220915201

GI number: 220915201

Start: 89592

End: 90788

Strand: Direct

Name: thlA [H]

Synonym: A2cp1_0080

Alternate gene names: 220915201

Gene position: 89592-90788 (Clockwise)

Preceding gene: 220915200

Following gene: 220915202

Centisome position: 1.78

GC content: 75.52

Gene sequence:

>1197_bases
ATGAACCGGGCCGGCCAGCAGGACGTGGTGTTCCTCGCCGCGAAGCGGACGCCCTTCGGGACGTTCGGCGGCTCCCTGAA
GGACCTCAGCGCGACCGACCTCGCGGTCCACGCCGCGCAGGCGGCGATCGAGCAGGCGGGGGCGCCGCCCGCGGACTACG
GCCACGTGGTGGTCGGGAACGTGGCCCAGACCTCGCCGGACGCGATCTACCTGGCGCGCCACGTCGGGCTGCGCGCCGGC
CTCCCCGAGGGCGTGCCCGCGGTCACCGTGAACCGTCTGTGCGGCTCCGGCTTCGAGGCGGTCATCGAGGCGGCGCGGCT
CATCCACGGCGGGGAGGCGGAGCTGGTGCTGGCCGGCGGCACCGAGTCGATGAGCCAGGCGCCGCTCGTGCTGCGCGGGA
CGCGCTTCGGCCACGCGTTCGGCAAGACGCCGCCGCTCGAGGACATGCTCTGGTCGGCGCTCACCGACACCTACGCCGGC
ATGCCCATGGCGCTCACCGCCGAGAAGCTGGCCGAGCGCCACGGGATCGGGCAGGACGCGGTGGACGAGCTCGCGGTGTC
CTCGCAGCGGCGCTGGGCCGCCGCCGACGAGGCCGGGCGCTTCCGCGAGGAGCTGGCGCCGGTGGAGCTCCGGACGCGCA
AGGGGACGGTGCGGTTCGAGAAGGACGAGCACCCGCGCCCCGGCACCACGCTGGAGGGGCTGCGGAAGCTCCCCAAGGTG
TTCAAGGCCGACGGCGTCATCCACGCCGGCGCGGCGAGCGGTATCGCCGACGGCGCCGGGATGCTGGTGGTGGCCTCGCG
CGCCTACGCGGAGCGGCGCGGGCTGCGGCCGCTGGGACGCCTGGTGAACTGGGGCCACGCCGGCGTGGACCCCACCGTGA
TGGGCATCGGGCCGGTGCCGGCGGTCCGGAACGCGCTGGCCCGCGCCGGCGCCACGCTCGCCGATCTCGACCTGATCGAG
GTGAACGAGGCGTTCGCGCCGCAGGTCCTGGCGGTGGAGCAGGAGCTGGGGCTCCCACGCGACCGCACCAACGTGGACGG
CGGCGCCATCGCGCTCGGCCATCCGCTCGCCGCGAGCGGGGCCCGGATCACCATGCACCTGCTGTACGAGCTCCGTCGCC
GGAAGGCCCGGGCCGGCCTCGGCTCGGCCTGCATCGGCGGCGGCCAGGGCGTGGCGGTGATCCTGGAGCCGCTGTAG

Upstream 100 bases:

>100_bases
GTGCCGGCCCTGAACGTGGCGCTGGCGCTGGCCATCCTGGTCGTGGCCGTGAAAGGCCTGGCGGGCGCGCTTATCTCGAG
GTGACGCACCAGGAGGCGTC

Downstream 100 bases:

>100_bases
CGGCGACGTTCTTGCCCCCGCCCGGAGGGGACGATAGGGTCCCCGAGGGTCCGCGGCGAGGAGGGGCGACGATGACGAGC
TTCACCGGCGTGAAGGTCTT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNRAGQQDVVFLAAKRTPFGTFGGSLKDLSATDLAVHAAQAAIEQAGAPPADYGHVVVGNVAQTSPDAIYLARHVGLRAG
LPEGVPAVTVNRLCGSGFEAVIEAARLIHGGEAELVLAGGTESMSQAPLVLRGTRFGHAFGKTPPLEDMLWSALTDTYAG
MPMALTAEKLAERHGIGQDAVDELAVSSQRRWAAADEAGRFREELAPVELRTRKGTVRFEKDEHPRPGTTLEGLRKLPKV
FKADGVIHAGAASGIADGAGMLVVASRAYAERRGLRPLGRLVNWGHAGVDPTVMGIGPVPAVRNALARAGATLADLDLIE
VNEAFAPQVLAVEQELGLPRDRTNVDGGAIALGHPLAASGARITMHLLYELRRRKARAGLGSACIGGGQGVAVILEPL

Sequences:

>Translated_398_residues
MNRAGQQDVVFLAAKRTPFGTFGGSLKDLSATDLAVHAAQAAIEQAGAPPADYGHVVVGNVAQTSPDAIYLARHVGLRAG
LPEGVPAVTVNRLCGSGFEAVIEAARLIHGGEAELVLAGGTESMSQAPLVLRGTRFGHAFGKTPPLEDMLWSALTDTYAG
MPMALTAEKLAERHGIGQDAVDELAVSSQRRWAAADEAGRFREELAPVELRTRKGTVRFEKDEHPRPGTTLEGLRKLPKV
FKADGVIHAGAASGIADGAGMLVVASRAYAERRGLRPLGRLVNWGHAGVDPTVMGIGPVPAVRNALARAGATLADLDLIE
VNEAFAPQVLAVEQELGLPRDRTNVDGGAIALGHPLAASGARITMHLLYELRRRKARAGLGSACIGGGQGVAVILEPL
>Mature_398_residues
MNRAGQQDVVFLAAKRTPFGTFGGSLKDLSATDLAVHAAQAAIEQAGAPPADYGHVVVGNVAQTSPDAIYLARHVGLRAG
LPEGVPAVTVNRLCGSGFEAVIEAARLIHGGEAELVLAGGTESMSQAPLVLRGTRFGHAFGKTPPLEDMLWSALTDTYAG
MPMALTAEKLAERHGIGQDAVDELAVSSQRRWAAADEAGRFREELAPVELRTRKGTVRFEKDEHPRPGTTLEGLRKLPKV
FKADGVIHAGAASGIADGAGMLVVASRAYAERRGLRPLGRLVNWGHAGVDPTVMGIGPVPAVRNALARAGATLADLDLIE
VNEAFAPQVLAVEQELGLPRDRTNVDGGAIALGHPLAASGARITMHLLYELRRRKARAGLGSACIGGGQGVAVILEPL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=388, Percent_Identity=55.9278350515464, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI148539872, Length=403, Percent_Identity=39.9503722084367, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI4501853, Length=403, Percent_Identity=39.7022332506204, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4557237, Length=405, Percent_Identity=37.5308641975309, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI4504327, Length=433, Percent_Identity=28.175519630485, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI194353979, Length=396, Percent_Identity=28.7878787878788, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1788554, Length=397, Percent_Identity=44.5843828715365, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI87082165, Length=390, Percent_Identity=42.8205128205128, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1787663, Length=391, Percent_Identity=44.5012787723785, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=39.2947103274559, Blast_Score=247, Evalue=8e-67,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=30.7328605200946, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=53.8461538461538, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=34.020618556701, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17535921, Length=396, Percent_Identity=35.6060606060606, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=429, Percent_Identity=28.6713286713287, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=27.8606965174129, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=34.3283582089552, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=34.0852130325814, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=51.7766497461929, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=43.7340153452685, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24640423, Length=398, Percent_Identity=35.929648241206, Blast_Score=224, Evalue=8e-59,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=29.0697674418605, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41559; Mature: 41559

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRAGQQDVVFLAAKRTPFGTFGGSLKDLSATDLAVHAAQAAIEQAGAPPADYGHVVVGN
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
VAQTSPDAIYLARHVGLRAGLPEGVPAVTVNRLCGSGFEAVIEAARLIHGGEAELVLAGG
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECC
TESMSQAPLVLRGTRFGHAFGKTPPLEDMLWSALTDTYAGMPMALTAEKLAERHGIGQDA
CCCHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
VDELAVSSQRRWAAADEAGRFREELAPVELRTRKGTVRFEKDEHPRPGTTLEGLRKLPKV
HHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHH
FKADGVIHAGAASGIADGAGMLVVASRAYAERRGLRPLGRLVNWGHAGVDPTVMGIGPVP
HHCCCEEECCCCCCCCCCCCEEEEEHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCCHH
AVRNALARAGATLADLDLIEVNEAFAPQVLAVEQELGLPRDRTNVDGGAIALGHPLAASG
HHHHHHHHCCCCHHCCEEEECCHHHCCHHEEEHHHHCCCCCCCCCCCCEEEECCCCCCCC
ARITMHLLYELRRRKARAGLGSACIGGGQGVAVILEPL
CHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEECC
>Mature Secondary Structure
MNRAGQQDVVFLAAKRTPFGTFGGSLKDLSATDLAVHAAQAAIEQAGAPPADYGHVVVGN
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
VAQTSPDAIYLARHVGLRAGLPEGVPAVTVNRLCGSGFEAVIEAARLIHGGEAELVLAGG
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECC
TESMSQAPLVLRGTRFGHAFGKTPPLEDMLWSALTDTYAGMPMALTAEKLAERHGIGQDA
CCCHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
VDELAVSSQRRWAAADEAGRFREELAPVELRTRKGTVRFEKDEHPRPGTTLEGLRKLPKV
HHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHH
FKADGVIHAGAASGIADGAGMLVVASRAYAERRGLRPLGRLVNWGHAGVDPTVMGIGPVP
HHCCCEEECCCCCCCCCCCCEEEEEHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCCHH
AVRNALARAGATLADLDLIEVNEAFAPQVLAVEQELGLPRDRTNVDGGAIALGHPLAASG
HHHHHHHHCCCCHHCCEEEECCHHHCCHHEEEHHHHCCCCCCCCCCCCEEEECCCCCCCC
ARITMHLLYELRRRKARAGLGSACIGGGQGVAVILEPL
CHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]