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The map label for this gene is pcpB [H]

Identifier: 220915043

GI number: 220915043

Start: 418212

End: 420158

Strand: Reverse

Name: pcpB [H]

Synonym: Mnod_8211

Alternate gene names: 220915043

Gene position: 420158-418212 (Counterclockwise)

Preceding gene: 220915044

Following gene: 220915042

Centisome position: 91.72

GC content: 64.82

Gene sequence:

>1947_bases
ATGCAATTCCACCTCAACGGCTACAAGCCCGGAGATCCAGGCATTTCCGAGCCGGCGCGTCAGGATAATGCGATGCAACC
AGGGGGCGGTCTGCCCGAGCACGTGGACGTTCTCATCATAGGCTGCGGACCGACAGGTCTGACGCTCGCGACGCAACTCG
CCGCCTTTCCCGACATCACGACCCGGATCGTTGACCAAAAGCCGGGCAGGCTCCTCGTCGGCCAGGCCGACGGGATCGCG
TGCCGGACAATGGAGATGTTCGAGGCGTTCGGCTTCAGCGAGCGCGTCCTTAAGGAAGCCTACTGGGTTAACGAGACGTG
CTTCTGGAACCCCGACGACAGTCAACGGACCACGATCGTCCGCACCGGCCGGATCCAGGACACCGAGGATGGCTTGTCCG
AATTCCCGCACGTCATCATGAACCAGGCGCGGGTGCATGATTTTTATCTTGACGTCATGCGCAACTCTCCCAGCCGACTG
GAGCCGAGCTACGCGCGCAGCTTCTTGGATCTCAGCATTGCGCCCCTTGGGCTGGGTTGCTCGGGTGAACCGAATGGCTC
TTCCCGTTACCCTGTAACGGTCAGGCTGGAGCGGCTCGACGACGCGCACAGGGGCGAGGTGGAAACTGTACGCGCGCGGT
GCGTGGTCGGGTGCGACGGAGCCCACAACAGCGTGCGTAAGGCGCTTGGACGTGCGGTTCACGGCGACTCCGCCAATCAA
GCCTGGGGCGTCATGGAGGTCCTGGCGCTTACGGACTTCCCCGACATCCGCCTCAAGACGGCCATCCACTCAGCGAACGA
AGGCAGCCTTCTCATCATCCCTCGCGAGGGCGGCTACCTGGTTCGGATGTACATCGAACTCGACAAGCTCAATGCGAACG
AGCGCGTGGCGGATCGCGCCATCACGGTCGACCATCTGATCGCGGCGGCGCAGCGCATCCTGCACCCCTACACCCTCGAG
GTGAAGGAAGTCGCGTGGTGGTCAGTCTACGAGATCGGCCAGCGGCCCTGTGACAAGTTCGACGACGTGCCGGAGGAGGA
CATCGATCGGCGCTGTCCCAGCGTGTTCATCGCCGGCGATGCGTGTCACACCCACAGCCCCAAGGCCGGACAGGGCATGA
ACGTCTCGATGCAGGATGCGTTCAACCTTGGCTGGAAGCTCGCCGCCGTCATCCGCGGGCACTGCTCGGCAGGCATCCTC
CACACCTACTCGGCGGAACGCCGCGCGGTCGCCCAGGAACTCATCGACTTCGACCGCAAGTTCGCGCGAATGTTCGGCGC
CGCGCCTAAGCGCCCCGGTGAGGCTGGGGGCGACGGCGTGGATCCGGCCGAGTTCCAGAGATACTTCCTCAAGCAAAGCC
GCTTCACGGCGGGGACGGAGACCTGCTACGCCCCCTCGCTCATCTCCGCCGAGCCTACCTACCAGCGCCTGGCCGAGGGT
CTGGTGATCGGCATGCGCTTCCACTCGGCGCCGGTGATCCGTCTGGCGGATGCCAGGCCGCTCCAGCTGGGTCATACAGT
CAAGGCCGACGGTCGCTGGCGTCTGTTTCTCTTCGCCGACGCGGAGGAGCCCGATGCCCCGTCATCGAAGTTGCGTGCGC
TGTGCACGTTCCTGGCCGAGGCCCCGGGCGCTCCCGTCCGGCGCTGCACGCCGGCCGGCGCCGACATCGATTCCGTCATT
GACGTGCGGCGATCTTCCAGCAGGGCCATCGCCAACTGGCCCCTGGTGCGATGCCGCCCCTTCTCCGGCCCCGGAAGGGC
CACTACGGCCTCGTCGATTACGAGAAGGTGTTCTGTCCCGACTTCAAAGCTGGCGACATTTTCGAGACGCGCGGCATCGT
GCGGGCGACCGGCTGCATCGTCGTCGTGCGACCGGACCAGTATGTCGCGCATGTCCTGCCGCTCGATGCTCATACCGAGC
TGCCGGCTTTCTTCGACAGGTTCATGA

Upstream 100 bases:

>100_bases
ATTTGTTGATTAGCATAGCCAGCAGCAACGATCAGCGTATTGGACCTGACGCATCGCACAGCAGATTCCCGGCCTAGCCA
CAAACCGAGAGATTGCGCCC

Downstream 100 bases:

>100_bases
CGTGAGCGACGAGGCAGTGCGATGACGGTTTCCGAAATCGATGCCATTCGGCACCTTCTGCTCGCGCAGCCGCGGCCGAC
GGACCCCGCCGCACGACGGG

Product: phenol 2-monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MQFHLNGYKPGDPGISEPARQDNAMQPGGGLPEHVDVLIIGCGPTGLTLATQLAAFPDITTRIVDQKPGRLLVGQADGIA
CRTMEMFEAFGFSERVLKEAYWVNETCFWNPDDSQRTTIVRTGRIQDTEDGLSEFPHVIMNQARVHDFYLDVMRNSPSRL
EPSYARSFLDLSIAPLGLGCSGEPNGSSRYPVTVRLERLDDAHRGEVETVRARCVVGCDGAHNSVRKALGRAVHGDSANQ
AWGVMEVLALTDFPDIRLKTAIHSANEGSLLIIPREGGYLVRMYIELDKLNANERVADRAITVDHLIAAAQRILHPYTLE
VKEVAWWSVYEIGQRPCDKFDDVPEEDIDRRCPSVFIAGDACHTHSPKAGQGMNVSMQDAFNLGWKLAAVIRGHCSAGIL
HTYSAERRAVAQELIDFDRKFARMFGAAPKRPGEAGGDGVDPAEFQRYFLKQSRFTAGTETCYAPSLISAEPTYQRLAEG
LVIGMRFHSAPVIRLADARPLQLGHTVKADGRWRLFLFADAEEPDAPSSKLRALCTFLAEAPGAPVRRCTPAGADIDSVI
DVRRSSSRAIANWPLVRCRPFSGPGRATTASSITRRCSVPTSKLATFSRRAASCGRPAASSSCDRTSMSRMSCRSMLIPS
CRLSSTGS

Sequences:

>Translated_648_residues
MQFHLNGYKPGDPGISEPARQDNAMQPGGGLPEHVDVLIIGCGPTGLTLATQLAAFPDITTRIVDQKPGRLLVGQADGIA
CRTMEMFEAFGFSERVLKEAYWVNETCFWNPDDSQRTTIVRTGRIQDTEDGLSEFPHVIMNQARVHDFYLDVMRNSPSRL
EPSYARSFLDLSIAPLGLGCSGEPNGSSRYPVTVRLERLDDAHRGEVETVRARCVVGCDGAHNSVRKALGRAVHGDSANQ
AWGVMEVLALTDFPDIRLKTAIHSANEGSLLIIPREGGYLVRMYIELDKLNANERVADRAITVDHLIAAAQRILHPYTLE
VKEVAWWSVYEIGQRPCDKFDDVPEEDIDRRCPSVFIAGDACHTHSPKAGQGMNVSMQDAFNLGWKLAAVIRGHCSAGIL
HTYSAERRAVAQELIDFDRKFARMFGAAPKRPGEAGGDGVDPAEFQRYFLKQSRFTAGTETCYAPSLISAEPTYQRLAEG
LVIGMRFHSAPVIRLADARPLQLGHTVKADGRWRLFLFADAEEPDAPSSKLRALCTFLAEAPGAPVRRCTPAGADIDSVI
DVRRSSSRAIANWPLVRCRPFSGPGRATTASSITRRCSVPTSKLATFSRRAASCGRPAASSSCDRTSMSRMSCRSMLIPS
CRLSSTGS
>Mature_648_residues
MQFHLNGYKPGDPGISEPARQDNAMQPGGGLPEHVDVLIIGCGPTGLTLATQLAAFPDITTRIVDQKPGRLLVGQADGIA
CRTMEMFEAFGFSERVLKEAYWVNETCFWNPDDSQRTTIVRTGRIQDTEDGLSEFPHVIMNQARVHDFYLDVMRNSPSRL
EPSYARSFLDLSIAPLGLGCSGEPNGSSRYPVTVRLERLDDAHRGEVETVRARCVVGCDGAHNSVRKALGRAVHGDSANQ
AWGVMEVLALTDFPDIRLKTAIHSANEGSLLIIPREGGYLVRMYIELDKLNANERVADRAITVDHLIAAAQRILHPYTLE
VKEVAWWSVYEIGQRPCDKFDDVPEEDIDRRCPSVFIAGDACHTHSPKAGQGMNVSMQDAFNLGWKLAAVIRGHCSAGIL
HTYSAERRAVAQELIDFDRKFARMFGAAPKRPGEAGGDGVDPAEFQRYFLKQSRFTAGTETCYAPSLISAEPTYQRLAEG
LVIGMRFHSAPVIRLADARPLQLGHTVKADGRWRLFLFADAEEPDAPSSKLRALCTFLAEAPGAPVRRCTPAGADIDSVI
DVRRSSSRAIANWPLVRCRPFSGPGRATTASSITRRCSVPTSKLATFSRRAASCGRPAASSSCDRTSMSRMSCRSMLIPS
CRLSSTGS

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=263, Percent_Identity=28.8973384030418, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 70968; Mature: 70968

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFHLNGYKPGDPGISEPARQDNAMQPGGGLPEHVDVLIIGCGPTGLTLATQLAAFPDIT
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHH
TRIVDQKPGRLLVGQADGIACRTMEMFEAFGFSERVLKEAYWVNETCFWNPDDSQRTTIV
HHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCEEEE
RTGRIQDTEDGLSEFPHVIMNQARVHDFYLDVMRNSPSRLEPSYARSFLDLSIAPLGLGC
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCEEEEECCCC
SGEPNGSSRYPVTVRLERLDDAHRGEVETVRARCVVGCDGAHNSVRKALGRAVHGDSANQ
CCCCCCCCCCCEEEEEECCCCCCCCCHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
AWGVMEVLALTDFPDIRLKTAIHSANEGSLLIIPREGGYLVRMYIELDKLNANERVADRA
HHHHHHHHHHCCCCCCEEEEEHCCCCCCCEEEEECCCCEEEEEEEEEECCCCCHHHHHHH
ITVDHLIAAAQRILHPYTLEVKEVAWWSVYEIGQRPCDKFDDVPEEDIDRRCPSVFIAGD
HHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHCCCCHHHHHCCCCEEEEECC
ACHTHSPKAGQGMNVSMQDAFNLGWKLAAVIRGHCSAGILHTYSAERRAVAQELIDFDRK
CCCCCCCCCCCCCCEEHHHHHCCCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHH
FARMFGAAPKRPGEAGGDGVDPAEFQRYFLKQSRFTAGTETCYAPSLISAEPTYQRLAEG
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
LVIGMRFHSAPVIRLADARPLQLGHTVKADGRWRLFLFADAEEPDAPSSKLRALCTFLAE
EEEEEEECCCCEEEECCCCCEECCCEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHH
APGAPVRRCTPAGADIDSVIDVRRSSSRAIANWPLVRCRPFSGPGRATTASSITRRCSVP
CCCCCCHHCCCCCCCHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCHHHHHHHHCCCC
TSKLATFSRRAASCGRPAASSSCDRTSMSRMSCRSMLIPSCRLSSTGS
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQFHLNGYKPGDPGISEPARQDNAMQPGGGLPEHVDVLIIGCGPTGLTLATQLAAFPDIT
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHH
TRIVDQKPGRLLVGQADGIACRTMEMFEAFGFSERVLKEAYWVNETCFWNPDDSQRTTIV
HHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCEEEE
RTGRIQDTEDGLSEFPHVIMNQARVHDFYLDVMRNSPSRLEPSYARSFLDLSIAPLGLGC
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCEEEEECCCC
SGEPNGSSRYPVTVRLERLDDAHRGEVETVRARCVVGCDGAHNSVRKALGRAVHGDSANQ
CCCCCCCCCCCEEEEEECCCCCCCCCHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
AWGVMEVLALTDFPDIRLKTAIHSANEGSLLIIPREGGYLVRMYIELDKLNANERVADRA
HHHHHHHHHHCCCCCCEEEEEHCCCCCCCEEEEECCCCEEEEEEEEEECCCCCHHHHHHH
ITVDHLIAAAQRILHPYTLEVKEVAWWSVYEIGQRPCDKFDDVPEEDIDRRCPSVFIAGD
HHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHCCCCHHHHHCCCCEEEEECC
ACHTHSPKAGQGMNVSMQDAFNLGWKLAAVIRGHCSAGILHTYSAERRAVAQELIDFDRK
CCCCCCCCCCCCCCEEHHHHHCCCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHH
FARMFGAAPKRPGEAGGDGVDPAEFQRYFLKQSRFTAGTETCYAPSLISAEPTYQRLAEG
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
LVIGMRFHSAPVIRLADARPLQLGHTVKADGRWRLFLFADAEEPDAPSSKLRALCTFLAE
EEEEEEECCCCEEEECCCCCEECCCEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHH
APGAPVRRCTPAGADIDSVIDVRRSSSRAIANWPLVRCRPFSGPGRATTASSITRRCSVP
CCCCCCHHCCCCCCCHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCHHHHHHHHCCCC
TSKLATFSRRAASCGRPAASSSCDRTSMSRMSCRSMLIPSCRLSSTGS
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]