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The map label for this gene is mchE [H]

Identifier: 220915002

GI number: 220915002

Start: 377607

End: 378938

Strand: Reverse

Name: mchE [H]

Synonym: Mnod_8164

Alternate gene names: 220915002

Gene position: 378938-377607 (Counterclockwise)

Preceding gene: 220915003

Following gene: 220915001

Centisome position: 82.72

GC content: 67.34

Gene sequence:

>1332_bases
ATGAGCCAGGATGGATCCGACTCCGAACTCGCTGCCGGCCAGAAACAGCTTCCCATCCACCGCGAGTCGCTGTTCCGGGC
GGAGGTCCTGGCCGAAGGGCAGGCGCAATGGCTCGGCACCGTGCTCCTGGAGCCGCGGCTCACCCATTGGATGTTCGCGC
TCTTTGCATCGGCGGCAGTGATGGCGATTGTCCTGCTGCTCGTCTTCGGCTCCTACACGCGCAAGGCGCGCATCAACGGC
TGGCTCGTGCCGGAGCAGGGCGTTGTCCGGAGCTTCTCCCCGCAGGCCGGCGTGATCACCGAGGTCCTCGTGCAGGAAGG
CATGGCGGTTCGGAAGGGCAGCCCGGTGCTGGTCATCTCGACGGAGATGCTGAGCAGTGCGGCCGGCGCCACCCGGCAGG
AGGTCGTCCGGCAGCTGAAACGGCGCCGTGACAGCATGATGCAGGAGAAGAGCGTTCAGGTGAGAGTGTTCGAGAACCAG
ATCAACGATGCCCGCAAGAAGCTGGACATCCTGAGCGCCGAGCGGAAGCATCTGGAAGTGGAGGTCGGCCTTCAGAAGAC
CAAGATCGAGATGGCCGACAGGACGGCGGTTCGCCTCCGCGAGTTGCGCAAGCGGGATATCGTCACCGCCCCCCGGCTGG
AGGAGGCGGAGAAGGAACGCCTCGACGAGGCCGCCAAGCTCCAGGCGCTGGAACGCAGCCTCGCAACTCTGGAGCGCGAA
CAGGCCGAGGCGTGGGCGACCCTGCAGGCCGTGCCGCTCCGCCGGCAGATCCAGGAGAACGAGAGCGAGCGCAGCGTCGC
GGCGCTGGAGCAGGACCTGATCGAGGCCGAGGCGCGGCGGGAGATCGTCATCACGGCTCCTCAGGATGGCACGATCACCG
GCCTTCAGGCCGAGCCCGGCGGCAACGCCACCACCTCGATTCCTCTCTTCGGCATCGTGCCGTCCCGGTCCAAGCTGCAG
GCGCAGCTCTTCAGCCCGAGCCGCGGCATCGGCTTCCTGCGCGCCGGGCAGCGGGTGCTGCTGCGCTATCAGGCCTTCCC
GTACCAGAAGTTCGGCTTCTACGGCGGGGTGGTGACCACCGTGTCCCGCTCCGCCCTGAGCCCGGCGGAGCTCAACCAGA
CCCTGGCCGGACTGACGGCGTTGTTCGGCAGCAACGAGCCCATCTATCGCGTCACCGTCGAGCTCGATCGCCAGACCGCC
GTCGCCTATGGGCAGGCCGTGGCGCTCCAGCCCGGCATGCAGCTGGAGGCGGACGTGCTGATCGAGAGCCGCCGCCTGAT
CGAGTGGGTGCTCGAACCTCTCTACACCTTGAGCGGATCATGGCACGCGTAA

Upstream 100 bases:

>100_bases
GCAAAGTGCTGCAGGTCCGCAGAACCTCGCAGGTCATCGAGCTGAGCTTCGAGGTGAAGGACTATTCCGTCAGGTCTCTC
GACGACGCATAGGCTGATCC

Downstream 100 bases:

>100_bases
GCGCCTTGGAGGGCCTGAACCTCGGCTGGGGCCGCCGCGTCCCGATGCTGCTCCAGACCGAGGCGGCCGAGTGCGGCCTG
GCCAGCCTGGCCATGGTGGC

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSQDGSDSELAAGQKQLPIHRESLFRAEVLAEGQAQWLGTVLLEPRLTHWMFALFASAAVMAIVLLLVFGSYTRKARING
WLVPEQGVVRSFSPQAGVITEVLVQEGMAVRKGSPVLVISTEMLSSAAGATRQEVVRQLKRRRDSMMQEKSVQVRVFENQ
INDARKKLDILSAERKHLEVEVGLQKTKIEMADRTAVRLRELRKRDIVTAPRLEEAEKERLDEAAKLQALERSLATLERE
QAEAWATLQAVPLRRQIQENESERSVAALEQDLIEAEARREIVITAPQDGTITGLQAEPGGNATTSIPLFGIVPSRSKLQ
AQLFSPSRGIGFLRAGQRVLLRYQAFPYQKFGFYGGVVTTVSRSALSPAELNQTLAGLTALFGSNEPIYRVTVELDRQTA
VAYGQAVALQPGMQLEADVLIESRRLIEWVLEPLYTLSGSWHA

Sequences:

>Translated_443_residues
MSQDGSDSELAAGQKQLPIHRESLFRAEVLAEGQAQWLGTVLLEPRLTHWMFALFASAAVMAIVLLLVFGSYTRKARING
WLVPEQGVVRSFSPQAGVITEVLVQEGMAVRKGSPVLVISTEMLSSAAGATRQEVVRQLKRRRDSMMQEKSVQVRVFENQ
INDARKKLDILSAERKHLEVEVGLQKTKIEMADRTAVRLRELRKRDIVTAPRLEEAEKERLDEAAKLQALERSLATLERE
QAEAWATLQAVPLRRQIQENESERSVAALEQDLIEAEARREIVITAPQDGTITGLQAEPGGNATTSIPLFGIVPSRSKLQ
AQLFSPSRGIGFLRAGQRVLLRYQAFPYQKFGFYGGVVTTVSRSALSPAELNQTLAGLTALFGSNEPIYRVTVELDRQTA
VAYGQAVALQPGMQLEADVLIESRRLIEWVLEPLYTLSGSWHA
>Mature_442_residues
SQDGSDSELAAGQKQLPIHRESLFRAEVLAEGQAQWLGTVLLEPRLTHWMFALFASAAVMAIVLLLVFGSYTRKARINGW
LVPEQGVVRSFSPQAGVITEVLVQEGMAVRKGSPVLVISTEMLSSAAGATRQEVVRQLKRRRDSMMQEKSVQVRVFENQI
NDARKKLDILSAERKHLEVEVGLQKTKIEMADRTAVRLRELRKRDIVTAPRLEEAEKERLDEAAKLQALERSLATLEREQ
AEAWATLQAVPLRRQIQENESERSVAALEQDLIEAEARREIVITAPQDGTITGLQAEPGGNATTSIPLFGIVPSRSKLQA
QLFSPSRGIGFLRAGQRVLLRYQAFPYQKFGFYGGVVTTVSRSALSPAELNQTLAGLTALFGSNEPIYRVTVELDRQTAV
AYGQAVALQPGMQLEADVLIESRRLIEWVLEPLYTLSGSWHA

Specific function: Probably involved, in conjunction with mchF, in the secretion of microcin H47 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 49177; Mature: 49046

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00543 HLYD_FAMILY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDGSDSELAAGQKQLPIHRESLFRAEVLAEGQAQWLGTVLLEPRLTHWMFALFASAAV
CCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
MAIVLLLVFGSYTRKARINGWLVPEQGVVRSFSPQAGVITEVLVQEGMAVRKGSPVLVIS
HHHHHHHHHCCCCCCEECCCEECCCCCCHHCCCCCCHHHHHHHHHCCCEEECCCCEEEEE
TEMLSSAAGATRQEVVRQLKRRRDSMMQEKSVQVRVFENQINDARKKLDILSAERKHLEV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHCEEE
EVGLQKTKIEMADRTAVRLRELRKRDIVTAPRLEEAEKERLDEAAKLQALERSLATLERE
EECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEAWATLQAVPLRRQIQENESERSVAALEQDLIEAEARREIVITAPQDGTITGLQAEPG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCC
GNATTSIPLFGIVPSRSKLQAQLFSPSRGIGFLRAGQRVLLRYQAFPYQKFGFYGGVVTT
CCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHCHHHHHHHHCCCCHHHHHCCHHHHH
VSRSALSPAELNQTLAGLTALFGSNEPIYRVTVELDRQTAVAYGQAVALQPGMQLEADVL
HHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCCEEEECCCCCCHHHHH
IESRRLIEWVLEPLYTLSGSWHA
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQDGSDSELAAGQKQLPIHRESLFRAEVLAEGQAQWLGTVLLEPRLTHWMFALFASAAV
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
MAIVLLLVFGSYTRKARINGWLVPEQGVVRSFSPQAGVITEVLVQEGMAVRKGSPVLVIS
HHHHHHHHHCCCCCCEECCCEECCCCCCHHCCCCCCHHHHHHHHHCCCEEECCCCEEEEE
TEMLSSAAGATRQEVVRQLKRRRDSMMQEKSVQVRVFENQINDARKKLDILSAERKHLEV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHCEEE
EVGLQKTKIEMADRTAVRLRELRKRDIVTAPRLEEAEKERLDEAAKLQALERSLATLERE
EECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAEAWATLQAVPLRRQIQENESERSVAALEQDLIEAEARREIVITAPQDGTITGLQAEPG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCC
GNATTSIPLFGIVPSRSKLQAQLFSPSRGIGFLRAGQRVLLRYQAFPYQKFGFYGGVVTT
CCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHCHHHHHHHHCCCCHHHHHCCHHHHH
VSRSALSPAELNQTLAGLTALFGSNEPIYRVTVELDRQTAVAYGQAVALQPGMQLEADVL
HHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCCEEEECCCCCCHHHHH
IESRRLIEWVLEPLYTLSGSWHA
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11181394 [H]