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The map label for this gene is mocB [H]

Identifier: 220914697

GI number: 220914697

Start: 35816

End: 36739

Strand: Reverse

Name: mocB [H]

Synonym: Mnod_7805

Alternate gene names: 220914697

Gene position: 36739-35816 (Counterclockwise)

Preceding gene: 220914698

Following gene: 220914696

Centisome position: 8.02

GC content: 65.04

Gene sequence:

>924_bases
ATGAAGCGACTTTTGCTTGCCGCGGCTCTGACTGCCTGCGCGCTCCCCGCCTATGCGGGGGGCCGGATCGGGGTCAGCAT
GACGTCACTCGACAACCCGTTCCTCACCATCCTGTTGAACGGAATGAAGGGTGAGGCCGCGCGAACCAAAGGCGTCGAGC
TGATGCTGGAGGACGCTCAGCGCGACGTGTCCCGCCAGTTGAGCCAAGTCCAGAACTTCGTCGCGAACCGCGTCGATGCC
ATTGTGGTGAACGCGGTCGATGGTGACTCCACCGCCGCGATCACCCGGGCTGCCAAGGCCGCCGGCATTCCGTTGGTTTA
TGTCAACCATCCGCCGGCGGAGCTGGGCCGTGGAATGCCGAAAGGCACCGCCTTCGTCGGCTCGAACGAGCTCGATTCAG
GCACGATGCAGGCCAGGGCCGTCTGCAAGATGCTCGCGGGCAAGGGCCGGGCCGTGATCCTGATGGGCCCGCTCGAAAAT
CATTCGGCGCTGGTGCGCACGAAGGATGTCGTAGATGTCTTTCGAACGCCGGACTGCCCGATCCACATCCTGGACAAGCA
GACCGCGAACTGGATGCGTGTCGAGGCGCAAGATCTGGTGGCCTCGTGGCTGACGGCGGGCATGAGGTTCGACGCGGTCA
TCGCCAACAGCGACGAGATGGCGATCGGCGCCGCGCAGGCGCTGAAGAGCGCGGGTGTGGCGATGAAGGACGTGGTGATT
GCGGGCATCGACGCAACGCCTGACGGACTCGCCGCCATGGCCACGGGCGACCTCGACGTCACCGTGTTCCAGAACGCCAC
CAGGCAGGGCGAGGTCGCGGTCCAGACCGCCGTCGCCATGGCGGCGGGCGAAAAGGCTGAGGACGCGATCTGGGTGCCGT
TCGAACTCGTCACCAAGGACAACCTGGCCGAGTACCGCAAGTAA

Upstream 100 bases:

>100_bases
CGTGTCCGCTTCTCTCTGAAAGCAGCGCTCCGGGACCAACAAAAAGAAGGCGTCGGCCCATGCCTGGGCCGGCAGCTGGT
CTGACGAAATGGAACTGGCC

Downstream 100 bases:

>100_bases
CCGGCCGAGGCGCGCGCGAGAGCCGCTGCTCTGTTTGCGCTGCGGCAGCAACCGACCGCCCCGGATTGTCGCACCAATGC
TGAGCCGGGATCACCGACAG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; beta-D-galactose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKRLLLAAALTACALPAYAGGRIGVSMTSLDNPFLTILLNGMKGEAARTKGVELMLEDAQRDVSRQLSQVQNFVANRVDA
IVVNAVDGDSTAAITRAAKAAGIPLVYVNHPPAELGRGMPKGTAFVGSNELDSGTMQARAVCKMLAGKGRAVILMGPLEN
HSALVRTKDVVDVFRTPDCPIHILDKQTANWMRVEAQDLVASWLTAGMRFDAVIANSDEMAIGAAQALKSAGVAMKDVVI
AGIDATPDGLAAMATGDLDVTVFQNATRQGEVAVQTAVAMAAGEKAEDAIWVPFELVTKDNLAEYRK

Sequences:

>Translated_307_residues
MKRLLLAAALTACALPAYAGGRIGVSMTSLDNPFLTILLNGMKGEAARTKGVELMLEDAQRDVSRQLSQVQNFVANRVDA
IVVNAVDGDSTAAITRAAKAAGIPLVYVNHPPAELGRGMPKGTAFVGSNELDSGTMQARAVCKMLAGKGRAVILMGPLEN
HSALVRTKDVVDVFRTPDCPIHILDKQTANWMRVEAQDLVASWLTAGMRFDAVIANSDEMAIGAAQALKSAGVAMKDVVI
AGIDATPDGLAAMATGDLDVTVFQNATRQGEVAVQTAVAMAAGEKAEDAIWVPFELVTKDNLAEYRK
>Mature_307_residues
MKRLLLAAALTACALPAYAGGRIGVSMTSLDNPFLTILLNGMKGEAARTKGVELMLEDAQRDVSRQLSQVQNFVANRVDA
IVVNAVDGDSTAAITRAAKAAGIPLVYVNHPPAELGRGMPKGTAFVGSNELDSGTMQARAVCKMLAGKGRAVILMGPLEN
HSALVRTKDVVDVFRTPDCPIHILDKQTANWMRVEAQDLVASWLTAGMRFDAVIANSDEMAIGAAQALKSAGVAMKDVVI
AGIDATPDGLAAMATGDLDVTVFQNATRQGEVAVQTAVAMAAGEKAEDAIWVPFELVTKDNLAEYRK

Specific function: Involved in rhizopine (L-3-O-methyl-scyllo-inosamine) catabolism. Could be involved in its high affinity transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788473, Length=331, Percent_Identity=29.0030211480363, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1790192, Length=301, Percent_Identity=29.5681063122924, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790674, Length=269, Percent_Identity=27.1375464684015, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789990, Length=249, Percent_Identity=25.7028112449799, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790526, Length=268, Percent_Identity=25.7462686567164, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 4720 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32333; Mature: 32333

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLLAAALTACALPAYAGGRIGVSMTSLDNPFLTILLNGMKGEAARTKGVELMLEDAQ
CCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHCCHHHHHHHHH
RDVSRQLSQVQNFVANRVDAIVVNAVDGDSTAAITRAAKAAGIPLVYVNHPPAELGRGMP
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHCCCC
KGTAFVGSNELDSGTMQARAVCKMLAGKGRAVILMGPLENHSALVRTKDVVDVFRTPDCP
CCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHCCCCCC
IHILDKQTANWMRVEAQDLVASWLTAGMRFDAVIANSDEMAIGAAQALKSAGVAMKDVVI
EEEECCCCCCEEEEHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEHEEE
AGIDATPDGLAAMATGDLDVTVFQNATRQGEVAVQTAVAMAAGEKAEDAIWVPFELVTKD
ECCCCCCCCCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCC
NLAEYRK
CHHHHCC
>Mature Secondary Structure
MKRLLLAAALTACALPAYAGGRIGVSMTSLDNPFLTILLNGMKGEAARTKGVELMLEDAQ
CCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHCCHHHHHHHHH
RDVSRQLSQVQNFVANRVDAIVVNAVDGDSTAAITRAAKAAGIPLVYVNHPPAELGRGMP
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHCCCC
KGTAFVGSNELDSGTMQARAVCKMLAGKGRAVILMGPLENHSALVRTKDVVDVFRTPDCP
CCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHCCCCCC
IHILDKQTANWMRVEAQDLVASWLTAGMRFDAVIANSDEMAIGAAQALKSAGVAMKDVVI
EEEECCCCCCEEEEHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEHEEE
AGIDATPDGLAAMATGDLDVTVFQNATRQGEVAVQTAVAMAAGEKAEDAIWVPFELVTKD
ECCCCCCCCCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEECCC
NLAEYRK
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; beta-D-galactose [Periplasm]; H2O [C]

Specific reaction: ATP + beta-D-galactose [Periplasm] + H2O = ADP + phosphate + beta-D-galactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7845353 [H]