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The map label for this gene is sppA [H]

Identifier: 220905589

GI number: 220905589

Start: 2803101

End: 2804252

Strand: Direct

Name: sppA [H]

Synonym: Ddes_2329

Alternate gene names: 220905589

Gene position: 2803101-2804252 (Clockwise)

Preceding gene: 220905588

Following gene: 220905590

Centisome position: 97.55

GC content: 66.23

Gene sequence:

>1152_bases
GTGAGCGATAAAGACTTTTCCTATGACGAACCGCTGTCAATGACAGGCTCTCCGGCCTCTGCCGCCAACGGCAAGGTCAC
GAATGAGCATGGCCCGCAGGCGGAGCCTGTCCGGGCACAAGCCCCGGTCGCAGCCCCGGCGGGCGCACCGGGCGCAACCC
CCTGCGCCACGGCCTGCCCGCTGGCGCAGATCCCCGCATCCGTGTGGAAAACACTGCTGCGCCGCCCCTTTCGCAAACGC
CATCCCGTACTGTTCTGGGCCGCCATAGTGATTGTTCTGGGCGCTCTGGGCGTTGTGGCCGGAAGCGCGGGCAAGGACGG
CGGCCTCATGGGCGGCGACCGTATTGCCCTGGTCAGTGTAACCGGCCCCATCATGAATGTGGAGCCTACCCTGGAATGGC
TGCGCACGGTGGCCCGCAATCCGTCGGTCAAGGGCGTGCTTGTGCGGGTGGATTCGCCCGGCGGCGGGGCAGCTGCCTCG
CAGGAAGTATATGACGCCCTGAAAAATCTGGCGCAAAAAATGCCCGTGGCCGTCAGCATGGGGTCCATGGCGGCCTCCGG
CGGGCTGATGGTCAGCATGGCCGGTCAGCGGGTATTCGCCAATCCTTCCACGGTTACCGGCTCCATCGGCGTACGCATGG
ACGTGCCACAACTGCAGGGCCTTATGGACAAGGTCGGCGTGGGGCAGGAAACTCTTGTGGTCGGCCAGTACAAGGACGCC
GCCTCCTACATGCGGCCCATGACGGCCGAGCAGCGCGCCTATTTTCAGGGTGTGCTTACCAACATGTATGACCAGTTTGT
GGACATTGTGGCCCAGGGGCGCGGCATGCCCCGCGACCGCGCACTCAAGCTCGCCAACGGCAAAGTGTACACCGGGCAGG
AGGCCCTGGGGCTGGGCCTTGTGGATGAACTGGGCGGACGCGGGCAGGCCCTCGCCTGGCTGGCCCAAAAGACCGGCGTG
CCTGCCGAGCGCAAACTGCTGACGCGCCCGCGTGAAGACGGCCTGCTCGGGCGCGGGCTGCGGGTCATGCTTGGCGCTGT
GCTCGGGCCTGAGTCCGATACTCTGGGCGGGCTGGCCTCTCTTGCGGGCCTTGCGCGGGGCGGCAGCGGCCATGCCGGCC
CGCAAACTCCGGCTTTTCTGTACCAGTTTTAA

Upstream 100 bases:

>100_bases
CGCCCGCCCGGCAGGGAAACGTTTTGCTGCCCACCTGCACGTGCGTTGCGCTTAACGGCAAAACAGGCAAAACGGCCATG
CCCGGTGCAAAGGATCATAC

Downstream 100 bases:

>100_bases
GATAATGCTGCGTTAACATTGCCCTGGCGGCTGCGTGAGCAGACGCTGCCTGCGGAGGCCGCAGGTCTGCACACCTGAGC
ACCAGCGTGAATGTCTTGCA

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSDKDFSYDEPLSMTGSPASAANGKVTNEHGPQAEPVRAQAPVAAPAGAPGATPCATACPLAQIPASVWKTLLRRPFRKR
HPVLFWAAIVIVLGALGVVAGSAGKDGGLMGGDRIALVSVTGPIMNVEPTLEWLRTVARNPSVKGVLVRVDSPGGGAAAS
QEVYDALKNLAQKMPVAVSMGSMAASGGLMVSMAGQRVFANPSTVTGSIGVRMDVPQLQGLMDKVGVGQETLVVGQYKDA
ASYMRPMTAEQRAYFQGVLTNMYDQFVDIVAQGRGMPRDRALKLANGKVYTGQEALGLGLVDELGGRGQALAWLAQKTGV
PAERKLLTRPREDGLLGRGLRVMLGAVLGPESDTLGGLASLAGLARGGSGHAGPQTPAFLYQF

Sequences:

>Translated_383_residues
MSDKDFSYDEPLSMTGSPASAANGKVTNEHGPQAEPVRAQAPVAAPAGAPGATPCATACPLAQIPASVWKTLLRRPFRKR
HPVLFWAAIVIVLGALGVVAGSAGKDGGLMGGDRIALVSVTGPIMNVEPTLEWLRTVARNPSVKGVLVRVDSPGGGAAAS
QEVYDALKNLAQKMPVAVSMGSMAASGGLMVSMAGQRVFANPSTVTGSIGVRMDVPQLQGLMDKVGVGQETLVVGQYKDA
ASYMRPMTAEQRAYFQGVLTNMYDQFVDIVAQGRGMPRDRALKLANGKVYTGQEALGLGLVDELGGRGQALAWLAQKTGV
PAERKLLTRPREDGLLGRGLRVMLGAVLGPESDTLGGLASLAGLARGGSGHAGPQTPAFLYQF
>Mature_382_residues
SDKDFSYDEPLSMTGSPASAANGKVTNEHGPQAEPVRAQAPVAAPAGAPGATPCATACPLAQIPASVWKTLLRRPFRKRH
PVLFWAAIVIVLGALGVVAGSAGKDGGLMGGDRIALVSVTGPIMNVEPTLEWLRTVARNPSVKGVLVRVDSPGGGAAASQ
EVYDALKNLAQKMPVAVSMGSMAASGGLMVSMAGQRVFANPSTVTGSIGVRMDVPQLQGLMDKVGVGQETLVVGQYKDAA
SYMRPMTAEQRAYFQGVLTNMYDQFVDIVAQGRGMPRDRALKLANGKVYTGQEALGLGLVDELGGRGQALAWLAQKTGVP
AERKLLTRPREDGLLGRGLRVMLGAVLGPESDTLGGLASLAGLARGGSGHAGPQTPAFLYQF

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=211, Percent_Identity=35.5450236966825, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787527, Length=172, Percent_Identity=31.3953488372093, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 39727; Mature: 39596

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKDFSYDEPLSMTGSPASAANGKVTNEHGPQAEPVRAQAPVAAPAGAPGATPCATACP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
LAQIPASVWKTLLRRPFRKRHPVLFWAAIVIVLGALGVVAGSAGKDGGLMGGDRIALVSV
HHHCCHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
TGPIMNVEPTLEWLRTVARNPSVKGVLVRVDSPGGGAAASQEVYDALKNLAQKMPVAVSM
CCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEC
GSMAASGGLMVSMAGQRVFANPSTVTGSIGVRMDVPQLQGLMDKVGVGQETLVVGQYKDA
CCHHCCCCEEEEECCCEEECCCCCEECCCCEEECCHHHHHHHHHHCCCCCEEEEECCHHH
ASYMRPMTAEQRAYFQGVLTNMYDQFVDIVAQGRGMPRDRALKLANGKVYTGQEALGLGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECCEEEECCHHHCCCC
VDELGGRGQALAWLAQKTGVPAERKLLTRPREDGLLGRGLRVMLGAVLGPESDTLGGLAS
HHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCHHCCHHHHHHHHCCCCCCHHHHHHH
LAGLARGGSGHAGPQTPAFLYQF
HHHHHCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
SDKDFSYDEPLSMTGSPASAANGKVTNEHGPQAEPVRAQAPVAAPAGAPGATPCATACP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
LAQIPASVWKTLLRRPFRKRHPVLFWAAIVIVLGALGVVAGSAGKDGGLMGGDRIALVSV
HHHCCHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
TGPIMNVEPTLEWLRTVARNPSVKGVLVRVDSPGGGAAASQEVYDALKNLAQKMPVAVSM
CCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEC
GSMAASGGLMVSMAGQRVFANPSTVTGSIGVRMDVPQLQGLMDKVGVGQETLVVGQYKDA
CCHHCCCCEEEEECCCEEECCCCCEECCCCEEECCHHHHHHHHHHCCCCCEEEEECCHHH
ASYMRPMTAEQRAYFQGVLTNMYDQFVDIVAQGRGMPRDRALKLANGKVYTGQEALGLGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECCEEEECCHHHCCCC
VDELGGRGQALAWLAQKTGVPAERKLLTRPREDGLLGRGLRVMLGAVLGPESDTLGGLAS
HHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCHHCCHHHHHHHHCCCCCCHHHHHHH
LAGLARGGSGHAGPQTPAFLYQF
HHHHHCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]