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The map label for this gene is hybA [C]

Identifier: 220905327

GI number: 220905327

Start: 2485967

End: 2486965

Strand: Direct

Name: hybA [C]

Synonym: Ddes_2065

Alternate gene names: 220905327

Gene position: 2485967-2486965 (Clockwise)

Preceding gene: 220905326

Following gene: 220905328

Centisome position: 86.52

GC content: 61.56

Gene sequence:

>999_bases
ATGGCAAAGGATTTTCTACCAGAACGCAGGCGCCTGCTCAAAGGGCTGACATCCGCCGCGGGAGTCGCCGGTCTCGGACT
AGCAGGCTTTCAGCCCGCCCAGGCCATGGCCCTGCGGCTGTCTACCGATGATGTGGACTGCTGTACCATTATTGATGTGG
ACCGTTGCACCGGCTGCGGTGCGTGTGTCGCTGCCTGCCGGGAGCGCAACCTTGCCGGCCTGCCGCTACCGCAGAAACCC
ATCCCCCGCCCGGTTCCCTCCTGGAAACCTGTCATCGACTGGTCTGAACGGCAGGATGTGACAAACCGCCTTACCCCGTA
CAACTGGCTGTACATCCAGTCCTGCACCCTTTCCGGCCGCCGGGAGGGACACAAGGTGTTCATGCCGCGACGCTGCCTGC
ATTGCCTCAATCCTCAATGTGTGAGCCTGTGCCCTTCCGGTGCGGCCCGGCAAAGCCCACAGGGGGCTGCATACATTGAT
GAATCCATGTGCCTGGGAGATGGCCCCTGCCATCGGGCCTGCCCCTGGGGCATTCCCCAACGCCAGTCAGGCGTTGGCCC
TTATCTCAAACTGGCACCCCGCTATATCGGTTATGGACTTGTATTCAAGTGCGATTATTGCCGGGACTTGCTGGCACAGG
GGCAGCCGCCCGTTTGTGTGGCTGCCTGCCCGTCCGGAGCACAAAGCATCGGCCCGCGCAACAAAATGGTGAGCCGTGCA
AAAGAAATGGCGAGCCTGCGGCGTGGAGAGCTCTTCGGCCTTACGGAAAACGGCGGAACCAACACCATCTACGTTTCTTC
CATCCCTTTCAGGGACATTGAAGCCGCCATGCTGCAGCAGGAACAGGTAGACTTTGGGCAGCCGAGCCTGCGCCCGGCCA
GGGCCAGTATGGAGAAAGAAAACAGCCTGCTTGATATCGTGCTGGCGGCCCCCCTGGCCGGAGCCGCCCTTGCTTCATTG
CGCCTGTGGCGCGACCGGAAAAAGGAGCGCAAGCCATGA

Upstream 100 bases:

>100_bases
CATTTCTGCCGGGCCGCGCGTTGACGCGAACATCTCGCTACGCGTACTATGTGTATACAATCCATAAAAAGACATGGCAA
AGAATCTCTCAAGGGGCAGC

Downstream 100 bases:

>100_bases
TGCGGTCGCAGCACCGGAACCGGAAGCTTTTTTCCGGCCTGTTTCATATCCTGTGGCCTGCAAGCCTGATGGCCGCAGTG
CTTTCCGGCCTGGCCCATTT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAKDFLPERRRLLKGLTSAAGVAGLGLAGFQPAQAMALRLSTDDVDCCTIIDVDRCTGCGACVAACRERNLAGLPLPQKP
IPRPVPSWKPVIDWSERQDVTNRLTPYNWLYIQSCTLSGRREGHKVFMPRRCLHCLNPQCVSLCPSGAARQSPQGAAYID
ESMCLGDGPCHRACPWGIPQRQSGVGPYLKLAPRYIGYGLVFKCDYCRDLLAQGQPPVCVAACPSGAQSIGPRNKMVSRA
KEMASLRRGELFGLTENGGTNTIYVSSIPFRDIEAAMLQQEQVDFGQPSLRPARASMEKENSLLDIVLAAPLAGAALASL
RLWRDRKKERKP

Sequences:

>Translated_332_residues
MAKDFLPERRRLLKGLTSAAGVAGLGLAGFQPAQAMALRLSTDDVDCCTIIDVDRCTGCGACVAACRERNLAGLPLPQKP
IPRPVPSWKPVIDWSERQDVTNRLTPYNWLYIQSCTLSGRREGHKVFMPRRCLHCLNPQCVSLCPSGAARQSPQGAAYID
ESMCLGDGPCHRACPWGIPQRQSGVGPYLKLAPRYIGYGLVFKCDYCRDLLAQGQPPVCVAACPSGAQSIGPRNKMVSRA
KEMASLRRGELFGLTENGGTNTIYVSSIPFRDIEAAMLQQEQVDFGQPSLRPARASMEKENSLLDIVLAAPLAGAALASL
RLWRDRKKERKP
>Mature_331_residues
AKDFLPERRRLLKGLTSAAGVAGLGLAGFQPAQAMALRLSTDDVDCCTIIDVDRCTGCGACVAACRERNLAGLPLPQKPI
PRPVPSWKPVIDWSERQDVTNRLTPYNWLYIQSCTLSGRREGHKVFMPRRCLHCLNPQCVSLCPSGAARQSPQGAAYIDE
SMCLGDGPCHRACPWGIPQRQSGVGPYLKLAPRYIGYGLVFKCDYCRDLLAQGQPPVCVAACPSGAQSIGPRNKMVSRAK
EMASLRRGELFGLTENGGTNTIYVSSIPFRDIEAAMLQQEQVDFGQPSLRPARASMEKENSLLDIVLAAPLAGAALASLR
LWRDRKKERKP

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=265, Percent_Identity=26.7924528301887, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787122, Length=203, Percent_Identity=29.064039408867, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787872, Length=197, Percent_Identity=28.9340101522843, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787749, Length=229, Percent_Identity=31.8777292576419, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790326, Length=229, Percent_Identity=31.8777292576419, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI226510944, Length=221, Percent_Identity=28.9592760180996, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=226, Percent_Identity=28.7610619469027, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787740, Length=124, Percent_Identity=33.0645161290323, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787478, Length=125, Percent_Identity=32, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36255; Mature: 36124

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDFLPERRRLLKGLTSAAGVAGLGLAGFQPAQAMALRLSTDDVDCCTIIDVDRCTGCG
CCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHEEEEECCCCCCEEEEEECHHCCCHH
ACVAACRERNLAGLPLPQKPIPRPVPSWKPVIDWSERQDVTNRLTPYNWLYIQSCTLSGR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCC
REGHKVFMPRRCLHCLNPQCVSLCPSGAARQSPQGAAYIDESMCLGDGPCHRACPWGIPQ
CCCCEEECHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
RQSGVGPYLKLAPRYIGYGLVFKCDYCRDLLAQGQPPVCVAACPSGAQSIGPRNKMVSRA
CCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCEEEEECCCCHHHCCCHHHHHHHH
KEMASLRRGELFGLTENGGTNTIYVSSIPFRDIEAAMLQQEQVDFGQPSLRPARASMEKE
HHHHHHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHC
NSLLDIVLAAPLAGAALASLRLWRDRKKERKP
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKDFLPERRRLLKGLTSAAGVAGLGLAGFQPAQAMALRLSTDDVDCCTIIDVDRCTGCG
CCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHEEEEECCCCCCEEEEEECHHCCCHH
ACVAACRERNLAGLPLPQKPIPRPVPSWKPVIDWSERQDVTNRLTPYNWLYIQSCTLSGR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCC
REGHKVFMPRRCLHCLNPQCVSLCPSGAARQSPQGAAYIDESMCLGDGPCHRACPWGIPQ
CCCCEEECHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
RQSGVGPYLKLAPRYIGYGLVFKCDYCRDLLAQGQPPVCVAACPSGAQSIGPRNKMVSRA
CCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCEEEEECCCCHHHCCCHHHHHHHH
KEMASLRRGELFGLTENGGTNTIYVSSIPFRDIEAAMLQQEQVDFGQPSLRPARASMEKE
HHHHHHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHC
NSLLDIVLAAPLAGAALASLRLWRDRKKERKP
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]