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The map label for this gene is miaA

Identifier: 220905298

GI number: 220905298

Start: 2448366

End: 2449337

Strand: Direct

Name: miaA

Synonym: Ddes_2036

Alternate gene names: 220905298

Gene position: 2448366-2449337 (Clockwise)

Preceding gene: 220905297

Following gene: 220905300

Centisome position: 85.21

GC content: 66.46

Gene sequence:

>972_bases
ATGCCAAGCAACAGCATCTTCGGCGGGGCGGCACAGGCCGCTCCGCCATCCGAAGCACTGCCCGTCATCTGTCTTGCCGG
CCCCACAGGCTCAGGTAAAACAGCCGCAGCCCTTGCCGTGGCCGAGGCTCTTGGCGGTGAAGTCATCAATGCGGACTCGC
GCCAGGTATATGCCGATTTTCCCCGCATCACGGCTCAACCCTCTGCCGAGGAACTGGCCTGCTGCCCGCACCATCTGTAT
GGCTTTCTGCCCACAGCGCAAAAAATCAGTGCCGGGCGATGGGCTGCGCAGGCCCAGGCCACAGCGCGGCAGATTCTTTC
AAGAGGCAAAATCCCCCTGCTGGTGGGCGGCACGGGGCTTTACTTCCAGACCCTGCTGCACGGCACGGCCGAAATCCCCC
CGGTGGATCCGGCCCTCACCGCGGCTTTCACCGCCCGCCTGGAAGACGTCGGTTCGCAGGCCATGCACGCCGAACTGGCC
CGCGTGGACCCGGACTACGCAACCCGCATACATCCTAACGACAGGCAGCGCATCGTGCGCGCCCTTGAGGTGCATGCGGC
CACAGGCAAGCCCTTCACCTGGTGGCACAAGCACGCCATGAGCCGCCCGCCCTGCAACGGACCGCTGCTTGTGCTGGATG
CCCCCCTGCCCTGGCTGGAGCCGCGCCTTGCCCGCCGTCTGGACATGATGCTTGAATCCGGCGCTATGGATGAAGCCGCA
GCAGCACTCAAGAATTGTGATGATGACGATGCCCCCGGCTGGACGGGCATCGGCTGCGCAGAGGCCCTCGCCTTTCTGCG
GGGCCGCCTGGGTTTTGACGCATGCCGCGATCTCTGGCTGCGCAACACGCGGGCGTACGCCAAGCGCCAGCTCACCTGGT
TTCGCGCGCGGCCCGAGGCCGTGTGGCTGCCCCCCGACAATGTGGATGCCGTAGTCAAGGCCGCGGGGGTGCGCCTGCCA
GCGGGGGTGTGA

Upstream 100 bases:

>100_bases
GGCCCTTCTGGAAAAATACGAAAAAGGCGAGGTCGCCAAGGGCACCCCATGCCTTGCTCCGCCACACGGCGGATTCCGCA
CCTCCTGAAGCGATACCCCC

Downstream 100 bases:

>100_bases
CGCCCGCCGCGCAGGCGGCCCAAATAAGAAATAAAGAAAAAGCCCCCGCGCCCGCAGGGCGATAAAAATCCCCTCTCAAA
GGACAAGCCATTCCCCTGCC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPSNSIFGGAAQAAPPSEALPVICLAGPTGSGKTAAALAVAEALGGEVINADSRQVYADFPRITAQPSAEELACCPHHLY
GFLPTAQKISAGRWAAQAQATARQILSRGKIPLLVGGTGLYFQTLLHGTAEIPPVDPALTAAFTARLEDVGSQAMHAELA
RVDPDYATRIHPNDRQRIVRALEVHAATGKPFTWWHKHAMSRPPCNGPLLVLDAPLPWLEPRLARRLDMMLESGAMDEAA
AALKNCDDDDAPGWTGIGCAEALAFLRGRLGFDACRDLWLRNTRAYAKRQLTWFRARPEAVWLPPDNVDAVVKAAGVRLP
AGV

Sequences:

>Translated_323_residues
MPSNSIFGGAAQAAPPSEALPVICLAGPTGSGKTAAALAVAEALGGEVINADSRQVYADFPRITAQPSAEELACCPHHLY
GFLPTAQKISAGRWAAQAQATARQILSRGKIPLLVGGTGLYFQTLLHGTAEIPPVDPALTAAFTARLEDVGSQAMHAELA
RVDPDYATRIHPNDRQRIVRALEVHAATGKPFTWWHKHAMSRPPCNGPLLVLDAPLPWLEPRLARRLDMMLESGAMDEAA
AALKNCDDDDAPGWTGIGCAEALAFLRGRLGFDACRDLWLRNTRAYAKRQLTWFRARPEAVWLPPDNVDAVVKAAGVRLP
AGV
>Mature_322_residues
PSNSIFGGAAQAAPPSEALPVICLAGPTGSGKTAAALAVAEALGGEVINADSRQVYADFPRITAQPSAEELACCPHHLYG
FLPTAQKISAGRWAAQAQATARQILSRGKIPLLVGGTGLYFQTLLHGTAEIPPVDPALTAAFTARLEDVGSQAMHAELAR
VDPDYATRIHPNDRQRIVRALEVHAATGKPFTWWHKHAMSRPPCNGPLLVLDAPLPWLEPRLARRLDMMLESGAMDEAAA
ALKNCDDDDAPGWTGIGCAEALAFLRGRLGFDACRDLWLRNTRAYAKRQLTWFRARPEAVWLPPDNVDAVVKAAGVRLPA
GV

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=28.343949044586, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1790613, Length=300, Percent_Identity=36, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25144712, Length=303, Percent_Identity=28.0528052805281, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=32.2916666666667, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24649830, Length=335, Percent_Identity=28.3582089552239, Blast_Score=120, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_DESDA (B8J3C2)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480610.1
- GeneID:   7285752
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_2036
- HOGENOM:   HBG559459
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34569; Mature: 34438

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSNSIFGGAAQAAPPSEALPVICLAGPTGSGKTAAALAVAEALGGEVINADSRQVYADF
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCCEEEECC
PRITAQPSAEELACCPHHLYGFLPTAQKISAGRWAAQAQATARQILSRGKIPLLVGGTGL
CCCCCCCCHHHHHHCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCHH
YFQTLLHGTAEIPPVDPALTAAFTARLEDVGSQAMHAELARVDPDYATRIHPNDRQRIVR
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHH
ALEVHAATGKPFTWWHKHAMSRPPCNGPLLVLDAPLPWLEPRLARRLDMMLESGAMDEAA
HHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHH
AALKNCDDDDAPGWTGIGCAEALAFLRGRLGFDACRDLWLRNTRAYAKRQLTWFRARPEA
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCE
VWLPPDNVDAVVKAAGVRLPAGV
EECCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PSNSIFGGAAQAAPPSEALPVICLAGPTGSGKTAAALAVAEALGGEVINADSRQVYADF
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCCEEEECC
PRITAQPSAEELACCPHHLYGFLPTAQKISAGRWAAQAQATARQILSRGKIPLLVGGTGL
CCCCCCCCHHHHHHCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCHH
YFQTLLHGTAEIPPVDPALTAAFTARLEDVGSQAMHAELARVDPDYATRIHPNDRQRIVR
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHH
ALEVHAATGKPFTWWHKHAMSRPPCNGPLLVLDAPLPWLEPRLARRLDMMLESGAMDEAA
HHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHH
AALKNCDDDDAPGWTGIGCAEALAFLRGRLGFDACRDLWLRNTRAYAKRQLTWFRARPEA
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCE
VWLPPDNVDAVVKAAGVRLPAGV
EECCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA