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The map label for this gene is aegA [H]

Identifier: 220905246

GI number: 220905246

Start: 2389134

End: 2390555

Strand: Direct

Name: aegA [H]

Synonym: Ddes_1984

Alternate gene names: 220905246

Gene position: 2389134-2390555 (Clockwise)

Preceding gene: 220905245

Following gene: 220905247

Centisome position: 83.15

GC content: 61.67

Gene sequence:

>1422_bases
ATGGAAACTAAAACCCAAAAAAAGAAACCCATCGCTCCCAGGGTGGACATGCCCTGCCAGCCCGCCGAGGTGCGCCGCGC
CAACTTCGATGAAGTGGCCCTGGGCTACACCAAGGAAATGGCCATTGAAGAAGCCCGGCGCTGCCTGCAGTGCAAAAAGC
CCTTCTGCGTCAGCGGCTGCCCTGTGGAAGTGCCCATCCGCGACTTTATCCGTGAAGTGGCCCTGGGCAATATGGATGCG
GCCTACCGCATCATCAAGAGCACCAACAGCCTGCCCGCCGTGTGCGGCCGCGTTTGCCCGCAGGAACATCAGTGCGAAGG
CAAGTGCGTCCTCAAGGCCAAGGGCCAGCCTGTAGCTATCGGCCGCCTTGAGCGCTTTGTGGCCGACACCTATATAGCGA
CCACAGCCTGCGAGCAGGTTACCGGAACCAACGCCTGCGCCCTGCCCAGAGGCGACCTCAAGGTGGCCTGCATCGGCTCC
GGCCCCTCATCCCTCACCTGTGCGGGCGTCTGCGCCGCCGCAGGCATCAAGGTAGACGTATACGAAGCCCTGCACGAGGC
CGGTGGGGTGCTCATCTATGGCATTCCGGCGTTCCGCCTGCCCAAGAACGTGGTCGCCACAGAAATTGACGGCCTGCGCC
AGTCTGGTGTGGACTTTCACCTGAACGCCGTTGGCGGCCGTACCGTGGACGTGGACGAACTGCTTGAAAGCCATCATGCC
GTCTTTATCGGCGTGGGTGCTGGCCTGCCCGTATTCCTGGGCGTGCCGGGAGAAAATCTTGTGGGCGTATTCTCGGCCAA
TGAGTATCTTACCCGCGTAAACCTCGGCCGGGCCTACGACTTTCCCGGTCAGGACACCCCGGCCTTTCCCGGTAAAAACG
TCACCGTGTTCGGCGCGGGCAACGTAGCTATGGACGCGGCGCGCACAGCCCTGCGCATGGGCGCGGAAAGCGTGCACATT
GTCTACCGCCGCACCAGGGACGAAATGCCCGCCCGCCTTGAAGAAATAGAACATGCCGAAGAAGAGGGCGTACAGTTTGC
CATGCTTTCCGCGCCGGTGTGCTTTAACGGCGACAGCGAAATGCGCCTCACCTCCGTTACCCTGCAAAAAATGGAACTGG
GCGAACCAGACGCCTCCGGCCGCCGCCGCCCCGTTGCCATTGAAGGCGAAGTGTACGACCTGCCCACCGACCTCGCCATT
GTGGCTCTGGGCACACGCTCCAATCCCATACTTCTGGAAGCCACACCCAAGCTCAAGCTGAACAAGTGGGGCTATATCGA
GGCCAATGAAGAAACAGGCGAAACATCCATACCCAACGTCTTTGCCGGCGGCGACATCGTTACCGGCGCGGCCACCGTCA
TCCTTGCCATGGGCGCGGGGCGCAAGGCCGGACAGGAAATCGTAAGGCGCCTGCTGAAGTAG

Upstream 100 bases:

>100_bases
GACGGGCATGAAGTGGACTTTCCCGAACTGCGCCGCCGCCTGGCTGCCTACCGCGAACAGGAAAAGGTCTCCATCGCCGA
TTATCAGCTGAGGATTGCTC

Downstream 100 bases:

>100_bases
CCCGAGACACTGCGCTCAAACATCCGCGGGGGAGAAAGCTTCCACTGTCTGCGCGCGCCTCCGGGTTGCGGCAAGACAGG
AAAAGGCTTTCTCCCCCGGT

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
METKTQKKKPIAPRVDMPCQPAEVRRANFDEVALGYTKEMAIEEARRCLQCKKPFCVSGCPVEVPIRDFIREVALGNMDA
AYRIIKSTNSLPAVCGRVCPQEHQCEGKCVLKAKGQPVAIGRLERFVADTYIATTACEQVTGTNACALPRGDLKVACIGS
GPSSLTCAGVCAAAGIKVDVYEALHEAGGVLIYGIPAFRLPKNVVATEIDGLRQSGVDFHLNAVGGRTVDVDELLESHHA
VFIGVGAGLPVFLGVPGENLVGVFSANEYLTRVNLGRAYDFPGQDTPAFPGKNVTVFGAGNVAMDAARTALRMGAESVHI
VYRRTRDEMPARLEEIEHAEEEGVQFAMLSAPVCFNGDSEMRLTSVTLQKMELGEPDASGRRRPVAIEGEVYDLPTDLAI
VALGTRSNPILLEATPKLKLNKWGYIEANEETGETSIPNVFAGGDIVTGAATVILAMGAGRKAGQEIVRRLLK

Sequences:

>Translated_473_residues
METKTQKKKPIAPRVDMPCQPAEVRRANFDEVALGYTKEMAIEEARRCLQCKKPFCVSGCPVEVPIRDFIREVALGNMDA
AYRIIKSTNSLPAVCGRVCPQEHQCEGKCVLKAKGQPVAIGRLERFVADTYIATTACEQVTGTNACALPRGDLKVACIGS
GPSSLTCAGVCAAAGIKVDVYEALHEAGGVLIYGIPAFRLPKNVVATEIDGLRQSGVDFHLNAVGGRTVDVDELLESHHA
VFIGVGAGLPVFLGVPGENLVGVFSANEYLTRVNLGRAYDFPGQDTPAFPGKNVTVFGAGNVAMDAARTALRMGAESVHI
VYRRTRDEMPARLEEIEHAEEEGVQFAMLSAPVCFNGDSEMRLTSVTLQKMELGEPDASGRRRPVAIEGEVYDLPTDLAI
VALGTRSNPILLEATPKLKLNKWGYIEANEETGETSIPNVFAGGDIVTGAATVILAMGAGRKAGQEIVRRLLK
>Mature_473_residues
METKTQKKKPIAPRVDMPCQPAEVRRANFDEVALGYTKEMAIEEARRCLQCKKPFCVSGCPVEVPIRDFIREVALGNMDA
AYRIIKSTNSLPAVCGRVCPQEHQCEGKCVLKAKGQPVAIGRLERFVADTYIATTACEQVTGTNACALPRGDLKVACIGS
GPSSLTCAGVCAAAGIKVDVYEALHEAGGVLIYGIPAFRLPKNVVATEIDGLRQSGVDFHLNAVGGRTVDVDELLESHHA
VFIGVGAGLPVFLGVPGENLVGVFSANEYLTRVNLGRAYDFPGQDTPAFPGKNVTVFGAGNVAMDAARTALRMGAESVHI
VYRRTRDEMPARLEEIEHAEEEGVQFAMLSAPVCFNGDSEMRLTSVTLQKMELGEPDASGRRRPVAIEGEVYDLPTDLAI
VALGTRSNPILLEATPKLKLNKWGYIEANEETGETSIPNVFAGGDIVTGAATVILAMGAGRKAGQEIVRRLLK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=492, Percent_Identity=31.0975609756098, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI237757300, Length=134, Percent_Identity=35.0746268656716, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788811, Length=456, Percent_Identity=37.5, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI87082180, Length=456, Percent_Identity=38.1578947368421, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1789606, Length=464, Percent_Identity=36.2068965517241, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788468, Length=448, Percent_Identity=32.3660714285714, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789243, Length=462, Percent_Identity=32.034632034632, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71984108, Length=447, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=458, Percent_Identity=28.82096069869, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17543860, Length=163, Percent_Identity=30.6748466257669, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=473, Percent_Identity=28.5412262156448, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24640763, Length=478, Percent_Identity=29.2887029288703, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18858217, Length=478, Percent_Identity=29.2887029288703, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24665547, Length=479, Percent_Identity=29.2275574112735, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24665543, Length=479, Percent_Identity=29.2275574112735, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28574881, Length=479, Percent_Identity=29.2275574112735, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24665539, Length=479, Percent_Identity=29.2275574112735, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 50669; Mature: 50669

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKTQKKKPIAPRVDMPCQPAEVRRANFDEVALGYTKEMAIEEARRCLQCKKPFCVSGC
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCC
PVEVPIRDFIREVALGNMDAAYRIIKSTNSLPAVCGRVCPQEHQCEGKCVLKAKGQPVAI
CEECCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEH
GRLERFVADTYIATTACEQVTGTNACALPRGDLKVACIGSGPSSLTCAGVCAAAGIKVDV
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCEEHH
YEALHEAGGVLIYGIPAFRLPKNVVATEIDGLRQSGVDFHLNAVGGRTVDVDELLESHHA
HHHHHHHCCEEEECCCCHHCCCHHHHHHHHHHHHCCCCEEEEECCCCEECHHHHHHCCCE
VFIGVGAGLPVFLGVPGENLVGVFSANEYLTRVNLGRAYDFPGQDTPAFPGKNVTVFGAG
EEEECCCCCEEEEECCCCCEEEEEECCCEEEEEECCCEECCCCCCCCCCCCCCEEEEECC
NVAMDAARTALRMGAESVHIVYRRTRDEMPARLEEIEHAEEEGVQFAMLSAPVCFNGDSE
CHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCC
MRLTSVTLQKMELGEPDASGRRRPVAIEGEVYDLPTDLAIVALGTRSNPILLEATPKLKL
EEEEEEEHHHHHCCCCCCCCCCCCEEECCEEEECCCCEEEEEECCCCCCEEEEECCCEEE
NKWGYIEANEETGETSIPNVFAGGDIVTGAATVILAMGAGRKAGQEIVRRLLK
CCCEEEECCCCCCCCCCCCEEECCCCHHHHHEEEEEECCCCHHHHHHHHHHHC
>Mature Secondary Structure
METKTQKKKPIAPRVDMPCQPAEVRRANFDEVALGYTKEMAIEEARRCLQCKKPFCVSGC
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCC
PVEVPIRDFIREVALGNMDAAYRIIKSTNSLPAVCGRVCPQEHQCEGKCVLKAKGQPVAI
CEECCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEH
GRLERFVADTYIATTACEQVTGTNACALPRGDLKVACIGSGPSSLTCAGVCAAAGIKVDV
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHCCCEEHH
YEALHEAGGVLIYGIPAFRLPKNVVATEIDGLRQSGVDFHLNAVGGRTVDVDELLESHHA
HHHHHHHCCEEEECCCCHHCCCHHHHHHHHHHHHCCCCEEEEECCCCEECHHHHHHCCCE
VFIGVGAGLPVFLGVPGENLVGVFSANEYLTRVNLGRAYDFPGQDTPAFPGKNVTVFGAG
EEEECCCCCEEEEECCCCCEEEEEECCCEEEEEECCCEECCCCCCCCCCCCCCEEEEECC
NVAMDAARTALRMGAESVHIVYRRTRDEMPARLEEIEHAEEEGVQFAMLSAPVCFNGDSE
CHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCC
MRLTSVTLQKMELGEPDASGRRRPVAIEGEVYDLPTDLAIVALGTRSNPILLEATPKLKL
EEEEEEEHHHHHCCCCCCCCCCCCEEECCEEEECCCCEEEEEECCCCCCEEEEECCCEEE
NKWGYIEANEETGETSIPNVFAGGDIVTGAATVILAMGAGRKAGQEIVRRLLK
CCCEEEECCCCCCCCCCCCEEECCCCHHHHHEEEEEECCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]