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The map label for this gene is cbiF [H]

Identifier: 220905242

GI number: 220905242

Start: 2383804

End: 2384667

Strand: Direct

Name: cbiF [H]

Synonym: Ddes_1980

Alternate gene names: 220905242

Gene position: 2383804-2384667 (Clockwise)

Preceding gene: 220905241

Following gene: 220905243

Centisome position: 82.96

GC content: 65.86

Gene sequence:

>864_bases
ATGACTACCTGCAAACATGACGAAAAGAATACGGCCCATATGACGGACGCAGCCGCAGGGCATGCTCCCGGCCAGACGCC
AGCCATAAACTCCGGCAGCACTCCCGGCCTGGTCAGCTTTGTCGGGGCCGGCCCCGGCGATCCGGAACTTTTGACGCTCA
AGGGGCGCAAGGCCATTGAAGAGGCCGCCCTGGTGCTTTATGCAGGTTCGCTGGTTCCGCCGCAGGTGGTGGCCTGCGCG
GCCCCCGATGTGCCTGTGGTGGACTCCGCCCCCCTGAGCCTTGAAGAATGCCACGCGCTGGTGCGCCGCACAGCCCTTGA
AGGCGGTACGGTGGCGCGTGTGCACACGGGCGATCCTGCCCTGTACGGCACGTTGCGCGAACAGACGCGCCTGCTGGATG
CAGACGGCATCCCCTGGCGCGTCATACCGGGCGTCACCGCGGCCTGTGCAGCGGCGGCCGCCGCAGGCGTAACCTTTACC
GTACCTGAAGTCACCCAGAGCCTCGTCATCACCCGGATGGAAGGACGCACCCCCGTGCCGGAACGCGAAGCCGTACGCCG
CCTTGCCGCTCACGGCACGTCCATGGCCATATACCTGTCGGCAGCATCCAGCGAGGCGTTGCAGGCCGACCTGCTGTGCC
ACATGCCGCCTGAAACACCGGTGCTGTGCGCTTGCCGCGTGGGCTGGCCGGACCAGCGCCTCATATGGGCCACCGCCGGA
ACCCTGGCCCGATGCGTTGCGGAGCACGGCCTGACCAGACAGACGGTTTTTCTGATACTGCCGGGGCAGAATGCGCCGGA
TACATGCTCGCAGCTCTACGCTTCCGGCTTCAGTCACGGCTACAGGCAGGCCAGAAAAAACTGA

Upstream 100 bases:

>100_bases
CCCCGGAGAGCACCGGCCGCGCCGGTCAGGGGGCGCGCTCATGAGACGCATCCCGAGCAGCGGCAACAGCACACCCGGCA
GACAACAGACAGCAGGCGCC

Downstream 100 bases:

>100_bases
ACCTTGCAGTCCGGCCCCGACAGACAGGTAAATGCGGGCGATACGTTCAGCCGCCCGCCGTGCAGACGGCCCGCCCGAAC
ACACTGAAACATTTCATATG

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTTCKHDEKNTAHMTDAAAGHAPGQTPAINSGSTPGLVSFVGAGPGDPELLTLKGRKAIEEAALVLYAGSLVPPQVVACA
APDVPVVDSAPLSLEECHALVRRTALEGGTVARVHTGDPALYGTLREQTRLLDADGIPWRVIPGVTAACAAAAAAGVTFT
VPEVTQSLVITRMEGRTPVPEREAVRRLAAHGTSMAIYLSAASSEALQADLLCHMPPETPVLCACRVGWPDQRLIWATAG
TLARCVAEHGLTRQTVFLILPGQNAPDTCSQLYASGFSHGYRQARKN

Sequences:

>Translated_287_residues
MTTCKHDEKNTAHMTDAAAGHAPGQTPAINSGSTPGLVSFVGAGPGDPELLTLKGRKAIEEAALVLYAGSLVPPQVVACA
APDVPVVDSAPLSLEECHALVRRTALEGGTVARVHTGDPALYGTLREQTRLLDADGIPWRVIPGVTAACAAAAAAGVTFT
VPEVTQSLVITRMEGRTPVPEREAVRRLAAHGTSMAIYLSAASSEALQADLLCHMPPETPVLCACRVGWPDQRLIWATAG
TLARCVAEHGLTRQTVFLILPGQNAPDTCSQLYASGFSHGYRQARKN
>Mature_286_residues
TTCKHDEKNTAHMTDAAAGHAPGQTPAINSGSTPGLVSFVGAGPGDPELLTLKGRKAIEEAALVLYAGSLVPPQVVACAA
PDVPVVDSAPLSLEECHALVRRTALEGGTVARVHTGDPALYGTLREQTRLLDADGIPWRVIPGVTAACAAAAAAGVTFTV
PEVTQSLVITRMEGRTPVPEREAVRRLAAHGTSMAIYLSAASSEALQADLLCHMPPETPVLCACRVGWPDQRLIWATAGT
LARCVAEHGLTRQTVFLILPGQNAPDTCSQLYASGFSHGYRQARKN

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=193, Percent_Identity=32.6424870466321, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 30026; Mature: 29894

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTCKHDEKNTAHMTDAAAGHAPGQTPAINSGSTPGLVSFVGAGPGDPELLTLKGRKAIE
CCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCHHHHH
EAALVLYAGSLVPPQVVACAAPDVPVVDSAPLSLEECHALVRRTALEGGTVARVHTGDPA
HHHEEEEECCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCH
LYGTLREQTRLLDADGIPWRVIPGVTAACAAAAAAGVTFTVPEVTQSLVITRMEGRTPVP
HHHHHHHHHHEECCCCCCEEECCCHHHHHHHHHHCCCEEEHHHHHHHHHEEECCCCCCCC
EREAVRRLAAHGTSMAIYLSAASSEALQADLLCHMPPETPVLCACRVGWPDQRLIWATAG
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHEEECCCCCCEEEEEECCCCCCEEEEEHHH
TLARCVAEHGLTRQTVFLILPGQNAPDTCSQLYASGFSHGYRQARKN
HHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTCKHDEKNTAHMTDAAAGHAPGQTPAINSGSTPGLVSFVGAGPGDPELLTLKGRKAIE
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCHHHHH
EAALVLYAGSLVPPQVVACAAPDVPVVDSAPLSLEECHALVRRTALEGGTVARVHTGDPA
HHHEEEEECCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCH
LYGTLREQTRLLDADGIPWRVIPGVTAACAAAAAAGVTFTVPEVTQSLVITRMEGRTPVP
HHHHHHHHHHEECCCCCCEEECCCHHHHHHHHHHCCCEEEHHHHHHHHHEEECCCCCCCC
EREAVRRLAAHGTSMAIYLSAASSEALQADLLCHMPPETPVLCACRVGWPDQRLIWATAG
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHEEECCCCCCEEEEEECCCCCCEEEEEHHH
TLARCVAEHGLTRQTVFLILPGQNAPDTCSQLYASGFSHGYRQARKN
HHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]