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The map label for this gene is pilJ [H]

Identifier: 220905157

GI number: 220905157

Start: 2284306

End: 2285985

Strand: Direct

Name: pilJ [H]

Synonym: Ddes_1895

Alternate gene names: 220905157

Gene position: 2284306-2285985 (Clockwise)

Preceding gene: 220905155

Following gene: 220905161

Centisome position: 79.5

GC content: 58.15

Gene sequence:

>1680_bases
ATGGGCATTATACTGCAACTCTTTATATTGTGTTCTCTCTGCGGCATGGTCTTTATGGAAAAACCGCAAGGCCTGCAATG
GCTGCTGCCTGCCTTTGCCGCAGCCTGTGCCGTGGCGGATGCGCTCCTTTGGTTAAAACGCAGGGCCGGGGACAAAAAAC
TGCGGGAGCAGAGCGACGCTCTTTTTGCCGAAGGCGCCCCCGCCCGTGAAGGCGACGTGGCGGAACGGGCCGAATACTGC
TTCAGCCACCTGCACGATGCCGAACAGGTTGTGGCGGAAGACGCCGAGGCCATCAAGGATATGCGCGCCATGGCCGAAGA
GTTCAAAACGCGTCTGGCCAGTGTTGAAGAAGAAAAAAAGAAGCTGCACAACAAATCCGAGCGCGGGGCCATGGTCCTGC
ACAAGGCGCATACCGTGTGCAGCAATCTTTCCACCGAGGTACGCGATCTGGCGAGCCTGGTCGTCGAGGTAAGCAAGGGC
GTTGAGTCGCAGCGCGCCAGCCTTACTGACACCAGCCGGGCCATGGAAGAGGTTTCACAGGCGGCTTTCGAATCTTCAAC
CAGGGTACAGGAACTTTCAGAAAGCGCCCAGCGCTCCAGCGCCAGTGCCGCCACAGGTGAACGCGAAGTGGAAGGCGCAC
TGTCTTCCATCGAAAAGCTCAAAGACATTATCGTGCAGCTCAAAACCGCCATGGCCGGACTGGGAGAAAAAGCCAGCAAC
ATCGGTCATGTCATGGCAGTCATCAACGAAGTGGCCGACCAGACCAACCTGTTGGCCCTCAACGCCGCCATTGAAGCCGC
CCGTGCCGGAGATGCCGGACGGGGTTTTGCCGTGGTGGCCGACGAGGTGCGCAAACTGGCCGAAAAAACCATGGGGGCCA
CCAAGGAAGTGGAAGAAGCCGTGCACGCCATACAGGCTGAAACACAGATCAATGTGCAGACTGTGGACAGGGCCGCCCGC
CTGAGCGTGGACGGCGCCGAACAGGCCGCGCGGGCCGGGGCCTTCATGCGTGATATTCTCCATGCCATGAACGAAACCGC
CGATCATCTCCAGACCATAGCCAAAAGCGTCAAGGAGCAGTCCGAGCAAAGCGTCGGCACCAGCGGCACCATGGGCGAAA
TCCGCCACATATCGGAACAGACGGCCGCCAGCATGGAAACATTCATCGCGGCCCTGCTGAGTTTCCAGAGCGGCATGGAA
GAACTGGACATGACCATCAACGCACTGGTTACCGGCGACTACGAGCAGGCCACCTCGGACAAGTTCGTCCAGTGGACGCC
CAAGCTCGACCTGCATGTGGCCATGGTGGACAGGCAGCACCGGCTGTTGGTTGACTATATCAACGAGCTGTACACGGCCA
TGGCCCACAACCGCACTTCAGAAGACCTTCAGGCCATCCTGCGCAAGCTGCGCGACTATACGGCCACACACTTCGCAGAT
GAAGAAAAACTGTTTACCGCCACCGCCTATCTGGGCGCGGCGGAGCATATGAAAATACACAGAAAATTTGTGGCAAAACT
GGACGAAGTGGAAGAGCAGCTCAAATCCGGCACTGCCACCGTGAGCATGGACCTGCTGACCTTCCTCAAGGACTGGCTCG
TACAGCACATTATGGGTACAGACCCCACATACCTGCCCTACCTCAAAAAAGAAGACTTCGAGCAGGATCATGGCCGCTAG

Upstream 100 bases:

>100_bases
AATATCAACCTCTTTGCTGTTTTGCGGTTTTATTTACATTTCCTGCTGTTAAGAGTAATTATACTATTGCCGATAAGCAA
GCAAACCAAGGCGGAAATAT

Downstream 100 bases:

>100_bases
GCCGTTTACAGAGCATCCGATGCCATGACGCGGCGTGTTTTCCACAACAGCAGGAAACCCCGGCATGGTAATGCCGGGAC
TCACGCGCAAACCGGCCTGA

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MGIILQLFILCSLCGMVFMEKPQGLQWLLPAFAAACAVADALLWLKRRAGDKKLREQSDALFAEGAPAREGDVAERAEYC
FSHLHDAEQVVAEDAEAIKDMRAMAEEFKTRLASVEEEKKKLHNKSERGAMVLHKAHTVCSNLSTEVRDLASLVVEVSKG
VESQRASLTDTSRAMEEVSQAAFESSTRVQELSESAQRSSASAATGEREVEGALSSIEKLKDIIVQLKTAMAGLGEKASN
IGHVMAVINEVADQTNLLALNAAIEAARAGDAGRGFAVVADEVRKLAEKTMGATKEVEEAVHAIQAETQINVQTVDRAAR
LSVDGAEQAARAGAFMRDILHAMNETADHLQTIAKSVKEQSEQSVGTSGTMGEIRHISEQTAASMETFIAALLSFQSGME
ELDMTINALVTGDYEQATSDKFVQWTPKLDLHVAMVDRQHRLLVDYINELYTAMAHNRTSEDLQAILRKLRDYTATHFAD
EEKLFTATAYLGAAEHMKIHRKFVAKLDEVEEQLKSGTATVSMDLLTFLKDWLVQHIMGTDPTYLPYLKKEDFEQDHGR

Sequences:

>Translated_559_residues
MGIILQLFILCSLCGMVFMEKPQGLQWLLPAFAAACAVADALLWLKRRAGDKKLREQSDALFAEGAPAREGDVAERAEYC
FSHLHDAEQVVAEDAEAIKDMRAMAEEFKTRLASVEEEKKKLHNKSERGAMVLHKAHTVCSNLSTEVRDLASLVVEVSKG
VESQRASLTDTSRAMEEVSQAAFESSTRVQELSESAQRSSASAATGEREVEGALSSIEKLKDIIVQLKTAMAGLGEKASN
IGHVMAVINEVADQTNLLALNAAIEAARAGDAGRGFAVVADEVRKLAEKTMGATKEVEEAVHAIQAETQINVQTVDRAAR
LSVDGAEQAARAGAFMRDILHAMNETADHLQTIAKSVKEQSEQSVGTSGTMGEIRHISEQTAASMETFIAALLSFQSGME
ELDMTINALVTGDYEQATSDKFVQWTPKLDLHVAMVDRQHRLLVDYINELYTAMAHNRTSEDLQAILRKLRDYTATHFAD
EEKLFTATAYLGAAEHMKIHRKFVAKLDEVEEQLKSGTATVSMDLLTFLKDWLVQHIMGTDPTYLPYLKKEDFEQDHGR
>Mature_558_residues
GIILQLFILCSLCGMVFMEKPQGLQWLLPAFAAACAVADALLWLKRRAGDKKLREQSDALFAEGAPAREGDVAERAEYCF
SHLHDAEQVVAEDAEAIKDMRAMAEEFKTRLASVEEEKKKLHNKSERGAMVLHKAHTVCSNLSTEVRDLASLVVEVSKGV
ESQRASLTDTSRAMEEVSQAAFESSTRVQELSESAQRSSASAATGEREVEGALSSIEKLKDIIVQLKTAMAGLGEKASNI
GHVMAVINEVADQTNLLALNAAIEAARAGDAGRGFAVVADEVRKLAEKTMGATKEVEEAVHAIQAETQINVQTVDRAARL
SVDGAEQAARAGAFMRDILHAMNETADHLQTIAKSVKEQSEQSVGTSGTMGEIRHISEQTAASMETFIAALLSFQSGMEE
LDMTINALVTGDYEQATSDKFVQWTPKLDLHVAMVDRQHRLLVDYINELYTAMAHNRTSEDLQAILRKLRDYTATHFADE
EKLFTATAYLGAAEHMKIHRKFVAKLDEVEEQLKSGTATVSMDLLTFLKDWLVQHIMGTDPTYLPYLKKEDFEQDHGR

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=241, Percent_Identity=31.9502074688797, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788195, Length=236, Percent_Identity=29.6610169491525, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788194, Length=208, Percent_Identity=32.2115384615385, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789453, Length=215, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI2367378, Length=206, Percent_Identity=31.5533980582524, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 61474; Mature: 61343

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00550 HEMERYTHRINS ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIILQLFILCSLCGMVFMEKPQGLQWLLPAFAAACAVADALLWLKRRAGDKKLREQSDA
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LFAEGAPAREGDVAERAEYCFSHLHDAEQVVAEDAEAIKDMRAMAEEFKTRLASVEEEKK
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLHNKSERGAMVLHKAHTVCSNLSTEVRDLASLVVEVSKGVESQRASLTDTSRAMEEVSQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
AAFESSTRVQELSESAQRSSASAATGEREVEGALSSIEKLKDIIVQLKTAMAGLGEKASN
HHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IGHVMAVINEVADQTNLLALNAAIEAARAGDAGRGFAVVADEVRKLAEKTMGATKEVEEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VHAIQAETQINVQTVDRAARLSVDGAEQAARAGAFMRDILHAMNETADHLQTIAKSVKEQ
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEQSVGTSGTMGEIRHISEQTAASMETFIAALLSFQSGMEELDMTINALVTGDYEQATSD
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCCC
KFVQWTPKLDLHVAMVDRQHRLLVDYINELYTAMAHNRTSEDLQAILRKLRDYTATHFAD
CCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
EEKLFTATAYLGAAEHMKIHRKFVAKLDEVEEQLKSGTATVSMDLLTFLKDWLVQHIMGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
DPTYLPYLKKEDFEQDHGR
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GIILQLFILCSLCGMVFMEKPQGLQWLLPAFAAACAVADALLWLKRRAGDKKLREQSDA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LFAEGAPAREGDVAERAEYCFSHLHDAEQVVAEDAEAIKDMRAMAEEFKTRLASVEEEKK
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLHNKSERGAMVLHKAHTVCSNLSTEVRDLASLVVEVSKGVESQRASLTDTSRAMEEVSQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
AAFESSTRVQELSESAQRSSASAATGEREVEGALSSIEKLKDIIVQLKTAMAGLGEKASN
HHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IGHVMAVINEVADQTNLLALNAAIEAARAGDAGRGFAVVADEVRKLAEKTMGATKEVEEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
VHAIQAETQINVQTVDRAARLSVDGAEQAARAGAFMRDILHAMNETADHLQTIAKSVKEQ
HHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEQSVGTSGTMGEIRHISEQTAASMETFIAALLSFQSGMEELDMTINALVTGDYEQATSD
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCCC
KFVQWTPKLDLHVAMVDRQHRLLVDYINELYTAMAHNRTSEDLQAILRKLRDYTATHFAD
CCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
EEKLFTATAYLGAAEHMKIHRKFVAKLDEVEEQLKSGTATVSMDLLTFLKDWLVQHIMGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
DPTYLPYLKKEDFEQDHGR
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]