Click here to switch to the map view.

The map label for this gene is hycG [H]

Identifier: 220905144

GI number: 220905144

Start: 2271865

End: 2272314

Strand: Direct

Name: hycG [H]

Synonym: Ddes_1882

Alternate gene names: 220905144

Gene position: 2271865-2272314 (Clockwise)

Preceding gene: 220905143

Following gene: 220905145

Centisome position: 79.06

GC content: 60.67

Gene sequence:

>450_bases
GTGTCGCTTGACAATATGCTTAAAAAACTTTCCGTGCGTTCGCCGTGGCTGTTCCGCATCAATGCCGGTTCCTGCAACGG
CTGTGACGTGGAACTGGCCACCACGGCCTGTATTCCGCGCTATGACGTGGAACGCCTCGGTTGCCGCTACTGCGGCAGCC
CGCGCCATGCCGATATTGTGCTTATTACCGGCCCCCTTACCACAAGGGTGCGCGACCGTGTGTTGCGCGTGTGGGATGAA
ATTCCCGAACCCAAGGTCACGGTGGCCGTGGGCATATGCCCCATATCCGGCGGGGTCTTCCGCGAAGGGTACTCCATTGA
AGGCCCCATTGACCGCTACCTGCCCGTGGATGTGAACGTACCCGGCTGCCCCCCGCGCCCCCAGGCCATACTTGAAGGCG
TAGTGCTGGCCCGGTCCATCTGGCTGAAAAAACTGGGCGTGGAGGAATAA

Upstream 100 bases:

>100_bases
CGTTTCCGCATTGACCAGGCGTTTCGCTTTTATGTGACTGTGCCCACAGCCCTTGCGCTGTGCAGCCTGATACTGGTCTG
GGTGATGTAAGGAGGCCGCC

Downstream 100 bases:

>100_bases
TATGGCGGGCTTTCTTAAAGTTTTATTCCGCAACCTGCTTGAAGGCCCCAGCACCGATCCTTTTCCTCTGGGCGAAACCT
TCACCCCCGAGCGCCTGCGC

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSLDNMLKKLSVRSPWLFRINAGSCNGCDVELATTACIPRYDVERLGCRYCGSPRHADIVLITGPLTTRVRDRVLRVWDE
IPEPKVTVAVGICPISGGVFREGYSIEGPIDRYLPVDVNVPGCPPRPQAILEGVVLARSIWLKKLGVEE

Sequences:

>Translated_149_residues
MSLDNMLKKLSVRSPWLFRINAGSCNGCDVELATTACIPRYDVERLGCRYCGSPRHADIVLITGPLTTRVRDRVLRVWDE
IPEPKVTVAVGICPISGGVFREGYSIEGPIDRYLPVDVNVPGCPPRPQAILEGVVLARSIWLKKLGVEE
>Mature_148_residues
SLDNMLKKLSVRSPWLFRINAGSCNGCDVELATTACIPRYDVERLGCRYCGSPRHADIVLITGPLTTRVRDRVLRVWDEI
PEPKVTVAVGICPISGGVFREGYSIEGPIDRYLPVDVNVPGCPPRPQAILEGVVLARSIWLKKLGVEE

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=114, Percent_Identity=39.4736842105263, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1789074, Length=141, Percent_Identity=42.5531914893617, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI1788834, Length=152, Percent_Identity=36.8421052631579, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1788624, Length=139, Percent_Identity=33.8129496402878, Blast_Score=85, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=108, Percent_Identity=39.8148148148148, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18859983, Length=108, Percent_Identity=39.8148148148148, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24642371, Length=108, Percent_Identity=39.8148148148148, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24651058, Length=108, Percent_Identity=37.962962962963, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 16459; Mature: 16328

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDNMLKKLSVRSPWLFRINAGSCNGCDVELATTACIPRYDVERLGCRYCGSPRHADIV
CCHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEE
LITGPLTTRVRDRVLRVWDEIPEPKVTVAVGICPISGGVFREGYSIEGPIDRYLPVDVNV
EEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCCCCCCEEEEECCC
PGCPPRPQAILEGVVLARSIWLKKLGVEE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLDNMLKKLSVRSPWLFRINAGSCNGCDVELATTACIPRYDVERLGCRYCGSPRHADIV
CHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEE
LITGPLTTRVRDRVLRVWDEIPEPKVTVAVGICPISGGVFREGYSIEGPIDRYLPVDVNV
EEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCCCCCCEEEEECCC
PGCPPRPQAILEGVVLARSIWLKKLGVEE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]