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The map label for this gene is pyrB

Identifier: 220905133

GI number: 220905133

Start: 2254818

End: 2255774

Strand: Reverse

Name: pyrB

Synonym: Ddes_1871

Alternate gene names: 220905133

Gene position: 2255774-2254818 (Counterclockwise)

Preceding gene: 220905138

Following gene: 220905132

Centisome position: 78.5

GC content: 61.34

Gene sequence:

>957_bases
ATGAATACCGACAATCGCTACCATTGGCCGCACAAGGACCTGCTGGATGTAACGCAGCTGAGCAGGGCGGATACCCTGCA
TCTGCTTGATCTGGCCGCCAGCTTTCAGGAAATAAACAGCCGCCCGGTCAAAAAGGTGCCCACTCTCAAGGGCAAGACCG
TGGTGCTTTTTTTTGTGGAAAACAGCACACGCACCAAAACTTCCTTTGATGTGGCGGGCAAGCGCCTTTCGGCAGACACG
TTTGCGCTGGGCAAGAGCGGCTCAAGCCTCAACAAGGGCGAAAGCCTCAAGGATACGGCCCTGACACTTCAGGCCATGTC
GCCGGATGTCATTGTCATGCGTCATTCCAGCAGCGGCGCGGCTCGCTATGTGGCAGACCTGCTGCCCTGCGGCGTGGTGA
ACGGCGGCGATGGCTGGCATGCCCATCCCACACAGGCGCTGCTGGACTGCTTCAGCCTGCGTGAAGCGTGGGCGAATTCC
TTTGAGGGCCGTACCCTGCTTATTCTGGGCGATATCGCACACAGCCGGGTGGCGCGTTCCAATATCCACCTGCTTACCAG
CCTTGGCGTACGCATACGGGTGTGCGCTCCGCGCACCCTGCTGCCTGCCGGGGTGGACCACTGGCCGGTGGAGGTATATA
CCGATCTGAGACAGGCGGTGCGCGATGTGGACGCCGTAATGTGCCTGCGTTTGCAACTGGAGCGCCAGCAGGCCGGGCTT
TTGCCCGATCTGGCCGAATATTCGCGGCGCTTCTGCCTTGGCCTTGAACACATGGCGCTGGCCCGGCCCGGCGCCGGTGT
GCTGCACCCCGGTCCCATGAACCGGGGACTGGAAATTTCAGACGATATGGCTGATGCCCCCGCAAGCCTGGTGCTTGATC
AGGTGGCTGCGGGCGTGGCCACGCGCATGGCAGTGCTGTATCTTCTGGCCACGCGCAATGATGGAGGACGCGCATGA

Upstream 100 bases:

>100_bases
TGCCGGATTTTTTGAAGTTTCCGCCGTTCAGGGCCGCAGCCGTACCCGTCGTTCCCCGTTGAAAAGCGGGGCTTTTTTTT
ACCTTTTACACAGCACTGCC

Downstream 100 bases:

>100_bases
GGCTTTGCATCAAAAATGCCCGCCACCTGGAAGCCCCGGTGGACCTTCTGGTGACCGGCGGAAAAATTACGACCATGACC
CCTGCGGGGCATCACACGCC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNTDNRYHWPHKDLLDVTQLSRADTLHLLDLAASFQEINSRPVKKVPTLKGKTVVLFFVENSTRTKTSFDVAGKRLSADT
FALGKSGSSLNKGESLKDTALTLQAMSPDVIVMRHSSSGAARYVADLLPCGVVNGGDGWHAHPTQALLDCFSLREAWANS
FEGRTLLILGDIAHSRVARSNIHLLTSLGVRIRVCAPRTLLPAGVDHWPVEVYTDLRQAVRDVDAVMCLRLQLERQQAGL
LPDLAEYSRRFCLGLEHMALARPGAGVLHPGPMNRGLEISDDMADAPASLVLDQVAAGVATRMAVLYLLATRNDGGRA

Sequences:

>Translated_318_residues
MNTDNRYHWPHKDLLDVTQLSRADTLHLLDLAASFQEINSRPVKKVPTLKGKTVVLFFVENSTRTKTSFDVAGKRLSADT
FALGKSGSSLNKGESLKDTALTLQAMSPDVIVMRHSSSGAARYVADLLPCGVVNGGDGWHAHPTQALLDCFSLREAWANS
FEGRTLLILGDIAHSRVARSNIHLLTSLGVRIRVCAPRTLLPAGVDHWPVEVYTDLRQAVRDVDAVMCLRLQLERQQAGL
LPDLAEYSRRFCLGLEHMALARPGAGVLHPGPMNRGLEISDDMADAPASLVLDQVAAGVATRMAVLYLLATRNDGGRA
>Mature_318_residues
MNTDNRYHWPHKDLLDVTQLSRADTLHLLDLAASFQEINSRPVKKVPTLKGKTVVLFFVENSTRTKTSFDVAGKRLSADT
FALGKSGSSLNKGESLKDTALTLQAMSPDVIVMRHSSSGAARYVADLLPCGVVNGGDGWHAHPTQALLDCFSLREAWANS
FEGRTLLILGDIAHSRVARSNIHLLTSLGVRIRVCAPRTLLPAGVDHWPVEVYTDLRQAVRDVDAVMCLRLQLERQQAGL
LPDLAEYSRRFCLGLEHMALARPGAGVLHPGPMNRGLEISDDMADAPASLVLDQVAAGVATRMAVLYLLATRNDGGRA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=302, Percent_Identity=32.7814569536424, Blast_Score=136, Evalue=3e-32,
Organism=Escherichia coli, GI2367364, Length=317, Percent_Identity=30.2839116719243, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790703, Length=328, Percent_Identity=26.5243902439024, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=27.5229357798165, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=29.7546012269939, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322331, Length=322, Percent_Identity=31.3664596273292, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24642586, Length=304, Percent_Identity=33.2236842105263, Blast_Score=128, Evalue=6e-30,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_DESDA (B8J2A8)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002480445.1
- GeneID:   7285586
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1871
- HOGENOM:   HBG579429
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34606; Mature: 34606

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDNRYHWPHKDLLDVTQLSRADTLHLLDLAASFQEINSRPVKKVPTLKGKTVVLFFVE
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
NSTRTKTSFDVAGKRLSADTFALGKSGSSLNKGESLKDTALTLQAMSPDVIVMRHSSSGA
CCCCCCCCHHHCCCCCCCCCEEECCCCCCCCCCCCCHHHHEEEEECCCCEEEEEECCCCH
ARYVADLLPCGVVNGGDGWHAHPTQALLDCFSLREAWANSFEGRTLLILGDIAHSRVARS
HHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHC
NIHLLTSLGVRIRVCAPRTLLPAGVDHWPVEVYTDLRQAVRDVDAVMCLRLQLERQQAGL
CCEEEEECCEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPDLAEYSRRFCLGLEHMALARPGAGVLHPGPMNRGLEISDDMADAPASLVLDQVAAGVA
CCCHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
TRMAVLYLLATRNDGGRA
HHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MNTDNRYHWPHKDLLDVTQLSRADTLHLLDLAASFQEINSRPVKKVPTLKGKTVVLFFVE
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
NSTRTKTSFDVAGKRLSADTFALGKSGSSLNKGESLKDTALTLQAMSPDVIVMRHSSSGA
CCCCCCCCHHHCCCCCCCCCEEECCCCCCCCCCCCCHHHHEEEEECCCCEEEEEECCCCH
ARYVADLLPCGVVNGGDGWHAHPTQALLDCFSLREAWANSFEGRTLLILGDIAHSRVARS
HHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHC
NIHLLTSLGVRIRVCAPRTLLPAGVDHWPVEVYTDLRQAVRDVDAVMCLRLQLERQQAGL
CCEEEEECCEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPDLAEYSRRFCLGLEHMALARPGAGVLHPGPMNRGLEISDDMADAPASLVLDQVAAGVA
CCCHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
TRMAVLYLLATRNDGGRA
HHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA