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The map label for this gene is fliM [H]

Identifier: 220905099

GI number: 220905099

Start: 2216848

End: 2217828

Strand: Direct

Name: fliM [H]

Synonym: Ddes_1836

Alternate gene names: 220905099

Gene position: 2216848-2217828 (Clockwise)

Preceding gene: 220905092

Following gene: 220905103

Centisome position: 77.15

GC content: 55.25

Gene sequence:

>981_bases
ATGAACAAAGTTTTGGCGCAAGATGAAGTGGATGCCCTGCTGCGCGGGCTTTCAGGCGGCGAAATCGAAAGCGAGGTTGA
AGTGCCGGAGGACGATTCCGGCATAGTGGCCTTTGACCTTGCCAATCAGGACCGCATCATACGCGGGCGCATGCCCGTGC
TCGAAATCGTCAATGACCGTTTTGCCCGGCTGTGCACCAACGCGCTTTCCAACTCTGTGCGCAAACGCGTGGAACTGAAC
CCCATATCCATTGATATGACAAAGTTCGGCGAATTCATGCGTTCCCTGCCCGTTCCCACTTCCATCAACATCTTCAAGAT
GGATCCGCTACGCGGCAATGCCATCATGGTGGTGGACTCACGCCTGGTCTTCGCCCTTGTGGAAAGCGTCTTCGGCGGCG
CAGGCTCCCAGCCCAAGGTGGAAGGACGCGAATTTACCCGCATTGAACAGGCCGTGGTCGACAAGATCGTCAAAATTGCC
CTGGACAATATGGAAGAATCCTGGCGACCCGTGCACGATGTCAAGCTGGAGCTGGTACGCAGCGAGATCAATCCGCAGTT
TGCGGCCATTGTGCCACCCAGCGATGTTGTGGTGGTCATCACCTTTGAAGTTGAGCTGGACACGGCCCTCGGCTCCATGA
TCATCTGCCTGCCCTACGCCACCATTGAACCCATCCGCTCCAAACTGCACGCCAGCTTTCAGACAGAACGGCTTGAGGTG
GACCATGCCTGGGTGGCGCGCCTTAAAGAGCGCCTTATGGAAACTCCGGTAGAACTCAAAGTATGCTTCGGCAAGACCAC
GATTACGGGCAACCAGCTTCTGCGCATGCAGGTAGGAGATATTCTGGTGCTGGACAAGGATGAGGAAGACATGCTCGACT
GCACTGTGGCGGGCGTGTGCAAGTATCAGGGCATTGCCGGAACAATCAAGGCCATGAAATCATTTCAGATTATCAAAGAA
AACGAGCCACAGTACACCTGA

Upstream 100 bases:

>100_bases
GACGCATTCCACAGCTTGTCAAAATGGCCTTTTTCTTGCATGGTTGCTCTTGCTGACGAAGAATACCAAGAACCATCACA
GGATGTTTGTCGAGGCTGGC

Downstream 100 bases:

>100_bases
TCATACAAGCACGGCAACCTGCACAGATGGGCTGACACAGACGGGCTGTACATGAACCGCCCTTGCGCTCTGGTGGCTTT
GCCGCCAGCCCGGCAACTGC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNKVLAQDEVDALLRGLSGGEIESEVEVPEDDSGIVAFDLANQDRIIRGRMPVLEIVNDRFARLCTNALSNSVRKRVELN
PISIDMTKFGEFMRSLPVPTSINIFKMDPLRGNAIMVVDSRLVFALVESVFGGAGSQPKVEGREFTRIEQAVVDKIVKIA
LDNMEESWRPVHDVKLELVRSEINPQFAAIVPPSDVVVVITFEVELDTALGSMIICLPYATIEPIRSKLHASFQTERLEV
DHAWVARLKERLMETPVELKVCFGKTTITGNQLLRMQVGDILVLDKDEEDMLDCTVAGVCKYQGIAGTIKAMKSFQIIKE
NEPQYT

Sequences:

>Translated_326_residues
MNKVLAQDEVDALLRGLSGGEIESEVEVPEDDSGIVAFDLANQDRIIRGRMPVLEIVNDRFARLCTNALSNSVRKRVELN
PISIDMTKFGEFMRSLPVPTSINIFKMDPLRGNAIMVVDSRLVFALVESVFGGAGSQPKVEGREFTRIEQAVVDKIVKIA
LDNMEESWRPVHDVKLELVRSEINPQFAAIVPPSDVVVVITFEVELDTALGSMIICLPYATIEPIRSKLHASFQTERLEV
DHAWVARLKERLMETPVELKVCFGKTTITGNQLLRMQVGDILVLDKDEEDMLDCTVAGVCKYQGIAGTIKAMKSFQIIKE
NEPQYT
>Mature_326_residues
MNKVLAQDEVDALLRGLSGGEIESEVEVPEDDSGIVAFDLANQDRIIRGRMPVLEIVNDRFARLCTNALSNSVRKRVELN
PISIDMTKFGEFMRSLPVPTSINIFKMDPLRGNAIMVVDSRLVFALVESVFGGAGSQPKVEGREFTRIEQAVVDKIVKIA
LDNMEESWRPVHDVKLELVRSEINPQFAAIVPPSDVVVVITFEVELDTALGSMIICLPYATIEPIRSKLHASFQTERLEV
DHAWVARLKERLMETPVELKVCFGKTTITGNQLLRMQVGDILVLDKDEEDMLDCTVAGVCKYQGIAGTIKAMKSFQIIKE
NEPQYT

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=319, Percent_Identity=35.423197492163, Blast_Score=194, Evalue=9e-51,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36391; Mature: 36391

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVLAQDEVDALLRGLSGGEIESEVEVPEDDSGIVAFDLANQDRIIRGRMPVLEIVNDR
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHH
FARLCTNALSNSVRKRVELNPISIDMTKFGEFMRSLPVPTSINIFKMDPLRGNAIMVVDS
HHHHHHHHHHHHHHHHEECCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECC
RLVFALVESVFGGAGSQPKVEGREFTRIEQAVVDKIVKIALDNMEESWRPVHDVKLELVR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SEINPQFAAIVPPSDVVVVITFEVELDTALGSMIICLPYATIEPIRSKLHASFQTERLEV
HHCCCCEEEEECCCCEEEEEEEEEEECHHHCCEEEEECCHHHHHHHHHHHCCHHHHHEEH
DHAWVARLKERLMETPVELKVCFGKTTITGNQLLRMQVGDILVLDKDEEDMLDCTVAGVC
HHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEECCEEEEECCCHHHHHHHHHHHH
KYQGIAGTIKAMKSFQIIKENEPQYT
HHCCHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MNKVLAQDEVDALLRGLSGGEIESEVEVPEDDSGIVAFDLANQDRIIRGRMPVLEIVNDR
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHH
FARLCTNALSNSVRKRVELNPISIDMTKFGEFMRSLPVPTSINIFKMDPLRGNAIMVVDS
HHHHHHHHHHHHHHHHEECCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECC
RLVFALVESVFGGAGSQPKVEGREFTRIEQAVVDKIVKIALDNMEESWRPVHDVKLELVR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SEINPQFAAIVPPSDVVVVITFEVELDTALGSMIICLPYATIEPIRSKLHASFQTERLEV
HHCCCCEEEEECCCCEEEEEEEEEEECHHHCCEEEEECCHHHHHHHHHHHCCHHHHHEEH
DHAWVARLKERLMETPVELKVCFGKTTITGNQLLRMQVGDILVLDKDEEDMLDCTVAGVC
HHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEEEECCEEEEECCCHHHHHHHHHHHH
KYQGIAGTIKAMKSFQIIKENEPQYT
HHCCHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]