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The map label for this gene is hycG [H]

Identifier: 220904936

GI number: 220904936

Start: 2007772

End: 2008329

Strand: Reverse

Name: hycG [H]

Synonym: Ddes_1671

Alternate gene names: 220904936

Gene position: 2008329-2007772 (Counterclockwise)

Preceding gene: 220904937

Following gene: 220904935

Centisome position: 69.89

GC content: 57.35

Gene sequence:

>558_bases
ATGGGTTTTGTAGACAAAATGATAAAGCGGAGCCGGCTCAAGTCGCCATGGATTGTCCATTTTGACTGCGGCTCCTGCAA
CGGTTGCGATATCGAGGTTTTGGCCTGCCTGACGCCCATGTATGATGTTGAGCGGTTCGGCGTGGTCAATGCGGGCAACC
CCAAGCATGCGGACGTATTGCTGGTGACCGGCACGGTAAATCACCGCAACCGCCACGTGCTCAAGCAGATCTACGAGCAG
ATGCCTTCTCCCAAGGCCGTCATTTCGCTCGGTGCCTGCAACCTTTCCGGCGGCGTTTTCAAAGATACTTACAATGTGCT
TAACGGCGCACATAACGTCATCCCTGTTGACGTGTTCGTGCCGGGCTGCCCGCCCAAACCCGAAGCCATCATTGATGGCG
TTGTGGAAGCTCTTGGGGTGCTCAAGGCCAAAATGGGCATGGGACCTGTGCCCCAGGCCACCTTCATGCCCGGCGACGAA
GATGGAACGCCCGCTGGCGCCAGGGACGAGGAAGCACCCGCTTCTGAAGGCGACAAGTCATTGCAAAACGCGGGTTAG

Upstream 100 bases:

>100_bases
TACCTGGCAGTGGATGGTGCAGAAGAAATCCCTGTTGCTCGGCATGGGGCTGGCCCTGGTTAATCTTTTATGGCTGTACG
TGGCGTAAGGAGGCAGGCAT

Downstream 100 bases:

>100_bases
CCCGGAAAGGATAAAGCCATGTTTTTTGAAGCCAAAGACGTGACGCCCGATACGCTGCTTGCTGAAGTGCAGCGCCTGGC
TAACGCCAAATGCCGGTTTG

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MGFVDKMIKRSRLKSPWIVHFDCGSCNGCDIEVLACLTPMYDVERFGVVNAGNPKHADVLLVTGTVNHRNRHVLKQIYEQ
MPSPKAVISLGACNLSGGVFKDTYNVLNGAHNVIPVDVFVPGCPPKPEAIIDGVVEALGVLKAKMGMGPVPQATFMPGDE
DGTPAGARDEEAPASEGDKSLQNAG

Sequences:

>Translated_185_residues
MGFVDKMIKRSRLKSPWIVHFDCGSCNGCDIEVLACLTPMYDVERFGVVNAGNPKHADVLLVTGTVNHRNRHVLKQIYEQ
MPSPKAVISLGACNLSGGVFKDTYNVLNGAHNVIPVDVFVPGCPPKPEAIIDGVVEALGVLKAKMGMGPVPQATFMPGDE
DGTPAGARDEEAPASEGDKSLQNAG
>Mature_184_residues
GFVDKMIKRSRLKSPWIVHFDCGSCNGCDIEVLACLTPMYDVERFGVVNAGNPKHADVLLVTGTVNHRNRHVLKQIYEQM
PSPKAVISLGACNLSGGVFKDTYNVLNGAHNVIPVDVFVPGCPPKPEAIIDGVVEALGVLKAKMGMGPVPQATFMPGDED
GTPAGARDEEAPASEGDKSLQNAG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=133, Percent_Identity=35.3383458646617, Blast_Score=117, Evalue=5e-27,
Organism=Escherichia coli, GI1789074, Length=160, Percent_Identity=43.75, Blast_Score=140, Evalue=4e-35,
Organism=Escherichia coli, GI1788834, Length=132, Percent_Identity=44.6969696969697, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1788624, Length=172, Percent_Identity=37.7906976744186, Blast_Score=110, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17509685, Length=144, Percent_Identity=38.1944444444444, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI18859983, Length=136, Percent_Identity=37.5, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24642371, Length=136, Percent_Identity=37.5, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24651058, Length=133, Percent_Identity=37.593984962406, Blast_Score=116, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 19656; Mature: 19525

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFVDKMIKRSRLKSPWIVHFDCGSCNGCDIEVLACLTPMYDVERFGVVNAGNPKHADVL
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHCCEEECCCCCCCEEE
LVTGTVNHRNRHVLKQIYEQMPSPKAVISLGACNLSGGVFKDTYNVLNGAHNVIPVDVFV
EEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEEE
PGCPPKPEAIIDGVVEALGVLKAKMGMGPVPQATFMPGDEDGTPAGARDEEAPASEGDKS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
LQNAG
HHCCC
>Mature Secondary Structure 
GFVDKMIKRSRLKSPWIVHFDCGSCNGCDIEVLACLTPMYDVERFGVVNAGNPKHADVL
CHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHCCEEECCCCCCCEEE
LVTGTVNHRNRHVLKQIYEQMPSPKAVISLGACNLSGGVFKDTYNVLNGAHNVIPVDVFV
EEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEEE
PGCPPKPEAIIDGVVEALGVLKAKMGMGPVPQATFMPGDEDGTPAGARDEEAPASEGDKS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
LQNAG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]