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The map label for this gene is cca [H]

Identifier: 220904822

GI number: 220904822

Start: 1868288

End: 1869352

Strand: Direct

Name: cca [H]

Synonym: Ddes_1555

Alternate gene names: 220904822

Gene position: 1868288-1869352 (Clockwise)

Preceding gene: 220904817

Following gene: 220904826

Centisome position: 65.02

GC content: 63.85

Gene sequence:

>1065_bases
ATGCTGCTCGGGCGTACGCCCGTGGAACTGGACTTCGCCTTTACCGGCAGCATGAAAAATTTTCTGGCCCGCCATCCGGA
TGCCAGGCTCGTGGGCAAAAGTGTGCATGTCTGCCTGTGGCGCGGCAGGGAATGTATGCCCCTGCGCGGCGGAGACATCG
AAGAAGATCTTGCGGCCCGCGACCTTACCATCAATGCCCTGGCGCTCGACAACGTGGGCAGGCTGCACATGCACCCGCAG
GCCCTTGAGGACCTGCGGCAGCAGATACTGCGCCCCGCCTCGCCTGCGGCCTTTGCGGCAGACCCCACTCGCATATTCCG
GCTGGCGCGCTTTGCCGCGGCATGGCCCGGCTGGCGCATTGACCGGAGTGCTTTTGAGCAGATGCGCGCCCTTTCTGCTC
AAACACTTGCCGCACTACCCGCGGAACGTGTGGGACGCGAAACCCTCAAGGCTCTTGCAGCGCCAGCTCCTGCGCGTTTT
TTCCGGGTGCTGGCCCAGGGCCATGCCCTGACCCCCTGGTTCACGGAGCTGGCAACCGCTGCAGGCATACCCGCCGGACC
AAGGCGCTGGCACAGCAATTCTGTTCTGGGACACAGTCTGCGCATTATGGATCTTGTGACCGGAGATCCCGTAGCAGCCT
GGATGGCCCTTTGCCACGATCTCGGCAAAATCCGGACAGATCCTGCGCTGCTGCCGCACCACTACGGCCATGAGCAGCGC
GGCGTTGCCCTGGCTGCGGGGCTGGCCCGCCGCCTACGTATGCCCGCGCGTTTTGCCAGGGCCGGAACACTGGCCGCCGC
CGAGCATATGAAAGGCGGCATGTATGCGGATCTGCGGCCCGGCACGAGACGCGACCTGCTCTGGCGCGTTCATGCGACAG
ACCTTGATTCCTCTTTCTGGCGGCTGGTGGATGCGGATCGGGGCAGCCCCGTCAGCACCGTTGCCCTGGCACACCTGGCC
GTTCTGCTTACTGTAAAACTGCCCGAAGACTGGCGCGACAAGGGAAAGCTTTCAGCAAAAAAACTGCGCGACCTGCATTG
CCAGGCCCTGGCAGAACAGCGTTAG

Upstream 100 bases:

>100_bases
AAAAAGCAGTGGCGAAGCAGGGTTGGCGCTTCCCTCACATGTCTGGCTATGGCATACTTCAGCCATGGAACTCTACCTCG
TTGGCGGCGCTGTGCGCGAC

Downstream 100 bases:

>100_bases
GGTTCTCTGCGTTCGCGAATATATGCTCTCAAAAAACCTGGGCGTTCACTGCGGCACATGACCACAAAACAGACAGTGAG
ACTACCTGTTTCACCCCCAG

Product: polynucleotide adenylyltransferase protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLLGRTPVELDFAFTGSMKNFLARHPDARLVGKSVHVCLWRGRECMPLRGGDIEEDLAARDLTINALALDNVGRLHMHPQ
ALEDLRQQILRPASPAAFAADPTRIFRLARFAAAWPGWRIDRSAFEQMRALSAQTLAALPAERVGRETLKALAAPAPARF
FRVLAQGHALTPWFTELATAAGIPAGPRRWHSNSVLGHSLRIMDLVTGDPVAAWMALCHDLGKIRTDPALLPHHYGHEQR
GVALAAGLARRLRMPARFARAGTLAAAEHMKGGMYADLRPGTRRDLLWRVHATDLDSSFWRLVDADRGSPVSTVALAHLA
VLLTVKLPEDWRDKGKLSAKKLRDLHCQALAEQR

Sequences:

>Translated_354_residues
MLLGRTPVELDFAFTGSMKNFLARHPDARLVGKSVHVCLWRGRECMPLRGGDIEEDLAARDLTINALALDNVGRLHMHPQ
ALEDLRQQILRPASPAAFAADPTRIFRLARFAAAWPGWRIDRSAFEQMRALSAQTLAALPAERVGRETLKALAAPAPARF
FRVLAQGHALTPWFTELATAAGIPAGPRRWHSNSVLGHSLRIMDLVTGDPVAAWMALCHDLGKIRTDPALLPHHYGHEQR
GVALAAGLARRLRMPARFARAGTLAAAEHMKGGMYADLRPGTRRDLLWRVHATDLDSSFWRLVDADRGSPVSTVALAHLA
VLLTVKLPEDWRDKGKLSAKKLRDLHCQALAEQR
>Mature_354_residues
MLLGRTPVELDFAFTGSMKNFLARHPDARLVGKSVHVCLWRGRECMPLRGGDIEEDLAARDLTINALALDNVGRLHMHPQ
ALEDLRQQILRPASPAAFAADPTRIFRLARFAAAWPGWRIDRSAFEQMRALSAQTLAALPAERVGRETLKALAAPAPARF
FRVLAQGHALTPWFTELATAAGIPAGPRRWHSNSVLGHSLRIMDLVTGDPVAAWMALCHDLGKIRTDPALLPHHYGHEQR
GVALAAGLARRLRMPARFARAGTLAAAEHMKGGMYADLRPGTRRDLLWRVHATDLDSSFWRLVDADRGSPVSTVALAHLA
VLLTVKLPEDWRDKGKLSAKKLRDLHCQALAEQR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=298, Percent_Identity=36.241610738255, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 39107; Mature: 39107

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGRTPVELDFAFTGSMKNFLARHPDARLVGKSVHVCLWRGRECMPLRGGDIEEDLAAR
CCCCCCCCEEEEEECCCHHHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHC
DLTINALALDNVGRLHMHPQALEDLRQQILRPASPAAFAADPTRIFRLARFAAAWPGWRI
CEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
DRSAFEQMRALSAQTLAALPAERVGRETLKALAAPAPARFFRVLAQGHALTPWFTELATA
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHH
AGIPAGPRRWHSNSVLGHSLRIMDLVTGDPVAAWMALCHDLGKIRTDPALLPHHYGHEQR
CCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GVALAAGLARRLRMPARFARAGTLAAAEHMKGGMYADLRPGTRRDLLWRVHATDLDSSFW
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCHHHH
RLVDADRGSPVSTVALAHLAVLLTVKLPEDWRDKGKLSAKKLRDLHCQALAEQR
HHHCCCCCCCHHHHHHHHHHHHHHEECCHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLGRTPVELDFAFTGSMKNFLARHPDARLVGKSVHVCLWRGRECMPLRGGDIEEDLAAR
CCCCCCCCEEEEEECCCHHHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHC
DLTINALALDNVGRLHMHPQALEDLRQQILRPASPAAFAADPTRIFRLARFAAAWPGWRI
CEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
DRSAFEQMRALSAQTLAALPAERVGRETLKALAAPAPARFFRVLAQGHALTPWFTELATA
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHH
AGIPAGPRRWHSNSVLGHSLRIMDLVTGDPVAAWMALCHDLGKIRTDPALLPHHYGHEQR
CCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GVALAAGLARRLRMPARFARAGTLAAAEHMKGGMYADLRPGTRRDLLWRVHATDLDSSFW
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCHHHH
RLVDADRGSPVSTVALAHLAVLLTVKLPEDWRDKGKLSAKKLRDLHCQALAEQR
HHHCCCCCCCHHHHHHHHHHHHHHEECCHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA