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The map label for this gene is braE [H]

Identifier: 220904748

GI number: 220904748

Start: 1771089

End: 1772312

Strand: Direct

Name: braE [H]

Synonym: Ddes_1480

Alternate gene names: 220904748

Gene position: 1771089-1772312 (Clockwise)

Preceding gene: 220904747

Following gene: 220904749

Centisome position: 61.64

GC content: 57.43

Gene sequence:

>1224_bases
ATGCAGAGAGTTATCAAGGCCGCCATAGCGGCACTCTGGTTCATGGTGCTGACCCTGCCCGTACTGGGCATCAAGCTGAA
CACCATCGAGAGCACTGTTACCTGGCGTCTGGACCGTATCGTTCTGCTGGGCGTGGGCATATTTGCGCTGGCCCTTATCT
GGGACTGGTGCTTCAGCCGAAAGGCCAGAGGCCTGTCCATCATCAGGCTGCCGCACAACTTCAGCCTCGGCTCGGGCATG
GCCGCCCTTTCCGAACGGCCCGCCCTCATGGCCGGAAGCCTGACTATTCTCGCAGTCATCATGATTGCCATGCCGCTGGT
GGTTTCATTTTACCAGACCAATATCATGATTTCGGCGCTGCTTTACATCATGCTGGCGCTGGGCCTGAACATCGTGGTGG
GCCTGGCCGGCCAGCTTGTGCTCGGCTATGTGGCTTTTTATGCCGTGGGCGCTTACGCCTACGGGCTGCTGCACCAGTTT
TTCGGCTGGGGTTTCTGGGTGTGCCTGCCGGTAGGCGGCTTTGTGGCCGTCATCTTCGGCCTGGCCCTAGGATTTCCCGT
GCTGCGGCTGCGTGGTGACTATCTGGCTATTGTTACCCTGGGTTTTGGCGAAATCGTGCGCCTGGCCCTGCAAAACTGGA
CCAGCCTTACCGGTGGCCCGCGCGGCGTGGGAGACATTCCCCGTCCCGGCTTCTTCGGCATGGACATGGATATCAGCACC
AGCACAACCTACGTGTATTACCTTGTGCTGGCAGCAGTGGCTATCACCATCATCGTCATCAGCCGCCTGAAAAACTCCAG
GGTGGGCCTTGCGCTACAGGCCTTGAGGGAAGATGAAATCGCCTGTGAAGCCATGGGAATAGATATCACACGGGTCAAAC
TTTCCGCATTTGCCCTTGGCTCGTGCTGGGCTGGTTTTGCCGGGGTTATCTTTGCGGCCAAAACGACCTACATCAATCCA
TCGAGCTTCACCTTTATGGAATCAGCCATGATTCTGTCCATGGTAGTGCTGGGCGGCATGGGTTCCATCACCGGCGTGGT
CATAGCCGCTCTTATTCTCATACTTGCGCCGGAATATCTGCGTGCCTTCTCTGAATACCGCATGCTCATCTTTGGAGCCA
TCATGGTAATCATGATGATTTTCCGGCCTCAGGGCCTTATCAGTGGCGAACGGCGCCGCTACCGCATCAGCGGCCTTCAT
GAGTCCAAAGGAGGCCGCCAGTGA

Upstream 100 bases:

>100_bases
GCAACTATGAAGACATGCTGGCCTTTGCCATCCTGATCCTCATTCTGATCTTCCGCCCCGACGGCATCCTGGGCAAAGCC
AAAGTGCAGAAGGTGTAGCC

Downstream 100 bases:

>100_bases
ACCCCGTTCTTGAAGTACAAGACCTTTCCCAGGACTTTGGCGGTCTGCGCGCTCTTAACGAGCTGTCGCTTACAGTCAAC
AGCGGCGAGATTGTGGCTCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MQRVIKAAIAALWFMVLTLPVLGIKLNTIESTVTWRLDRIVLLGVGIFALALIWDWCFSRKARGLSIIRLPHNFSLGSGM
AALSERPALMAGSLTILAVIMIAMPLVVSFYQTNIMISALLYIMLALGLNIVVGLAGQLVLGYVAFYAVGAYAYGLLHQF
FGWGFWVCLPVGGFVAVIFGLALGFPVLRLRGDYLAIVTLGFGEIVRLALQNWTSLTGGPRGVGDIPRPGFFGMDMDIST
STTYVYYLVLAAVAITIIVISRLKNSRVGLALQALREDEIACEAMGIDITRVKLSAFALGSCWAGFAGVIFAAKTTYINP
SSFTFMESAMILSMVVLGGMGSITGVVIAALILILAPEYLRAFSEYRMLIFGAIMVIMMIFRPQGLISGERRRYRISGLH
ESKGGRQ

Sequences:

>Translated_407_residues
MQRVIKAAIAALWFMVLTLPVLGIKLNTIESTVTWRLDRIVLLGVGIFALALIWDWCFSRKARGLSIIRLPHNFSLGSGM
AALSERPALMAGSLTILAVIMIAMPLVVSFYQTNIMISALLYIMLALGLNIVVGLAGQLVLGYVAFYAVGAYAYGLLHQF
FGWGFWVCLPVGGFVAVIFGLALGFPVLRLRGDYLAIVTLGFGEIVRLALQNWTSLTGGPRGVGDIPRPGFFGMDMDIST
STTYVYYLVLAAVAITIIVISRLKNSRVGLALQALREDEIACEAMGIDITRVKLSAFALGSCWAGFAGVIFAAKTTYINP
SSFTFMESAMILSMVVLGGMGSITGVVIAALILILAPEYLRAFSEYRMLIFGAIMVIMMIFRPQGLISGERRRYRISGLH
ESKGGRQ
>Mature_407_residues
MQRVIKAAIAALWFMVLTLPVLGIKLNTIESTVTWRLDRIVLLGVGIFALALIWDWCFSRKARGLSIIRLPHNFSLGSGM
AALSERPALMAGSLTILAVIMIAMPLVVSFYQTNIMISALLYIMLALGLNIVVGLAGQLVLGYVAFYAVGAYAYGLLHQF
FGWGFWVCLPVGGFVAVIFGLALGFPVLRLRGDYLAIVTLGFGEIVRLALQNWTSLTGGPRGVGDIPRPGFFGMDMDIST
STTYVYYLVLAAVAITIIVISRLKNSRVGLALQALREDEIACEAMGIDITRVKLSAFALGSCWAGFAGVIFAAKTTYINP
SSFTFMESAMILSMVVLGGMGSITGVVIAALILILAPEYLRAFSEYRMLIFGAIMVIMMIFRPQGLISGERRRYRISGLH
ESKGGRQ

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=313, Percent_Identity=48.5623003194888, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1789866, Length=289, Percent_Identity=27.3356401384083, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 44211; Mature: 44211

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRVIKAAIAALWFMVLTLPVLGIKLNTIESTVTWRLDRIVLLGVGIFALALIWDWCFSR
CHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KARGLSIIRLPHNFSLGSGMAALSERPALMAGSLTILAVIMIAMPLVVSFYQTNIMISAL
CCCCCEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYIMLALGLNIVVGLAGQLVLGYVAFYAVGAYAYGLLHQFFGWGFWVCLPVGGFVAVIFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALGFPVLRLRGDYLAIVTLGFGEIVRLALQNWTSLTGGPRGVGDIPRPGFFGMDMDIST
HHHCCHHHHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
STTYVYYLVLAAVAITIIVISRLKNSRVGLALQALREDEIACEAMGIDITRVKLSAFALG
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
SCWAGFAGVIFAAKTTYINPSSFTFMESAMILSMVVLGGMGSITGVVIAALILILAPEYL
HHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHH
RAFSEYRMLIFGAIMVIMMIFRPQGLISGERRRYRISGLHESKGGRQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MQRVIKAAIAALWFMVLTLPVLGIKLNTIESTVTWRLDRIVLLGVGIFALALIWDWCFSR
CHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KARGLSIIRLPHNFSLGSGMAALSERPALMAGSLTILAVIMIAMPLVVSFYQTNIMISAL
CCCCCEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYIMLALGLNIVVGLAGQLVLGYVAFYAVGAYAYGLLHQFFGWGFWVCLPVGGFVAVIFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALGFPVLRLRGDYLAIVTLGFGEIVRLALQNWTSLTGGPRGVGDIPRPGFFGMDMDIST
HHHCCHHHHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
STTYVYYLVLAAVAITIIVISRLKNSRVGLALQALREDEIACEAMGIDITRVKLSAFALG
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
SCWAGFAGVIFAAKTTYINPSSFTFMESAMILSMVVLGGMGSITGVVIAALILILAPEYL
HHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHH
RAFSEYRMLIFGAIMVIMMIFRPQGLISGERRRYRISGLHESKGGRQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]